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MSDS Material Safety Data Sheet
CAS

7647-14-5
6132-04-3

File Name: ambion_com---msds_9763.asp
AMBION, INC.                                                                             20X SSC Buffer pH 7.0
Catalog #: 9763, 9765, 9770
Material Safety Data Sheet
Section 1-Identification of Product
Catalog Number: 9763, 9765, For research Health: 1 Fire: 0 Stability: 0
9770 use only.
Synonyms:
Contact Information
Ambion, Inc 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800 888 8804 E-
mail: techserv@ambion.com Web address www.ambion.com

Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373
010 E-mail: eurotech@ambion.com Web address: www.ambion.com

Emergency Contact: In Europe, call 112. In USA, call 911
Section 2-Composition/Information
Hazardous Ingredients (Specific) % CAS # R-Statement S-Statement
Sodium Chloride 18 7647-14-5 R:36/37/38 S:36/37/39
Sodium Citrate 9 6132-04-3 N.A. N.A.
Section 3-Hazard Identification
Routes of Entry
Skin Contact Sodium Chloride: May irritate damaged skin; absorption can occur
with effects similar to those via ingestion.
Sodium Citrate: Possible irritation on prolonged contact with moist or
sensitive area of the skin.
Skin Absorption Sodium Chloride: May irritate damaged skin; absorption can occur
with effects similar to those via ingestion.
Sodium Citrate: Possible irritation or prolonged contact with moist or
sensitive area of the skin.
Eye Contact Sodium Chloride: Causes irritation, redness, and pain.
Sodium Citrate: No adverse effects expected but dust may cause
mechanical irritation.
Inhalation Sodium Chloride: May cause mild irritation to the respiratory tract.
Sodium Citrate: Inhalation of large amounts of dust may cause
irritation to the respiratory tract.
Ingestion Sodium Chloride: Very large doses can cause vomiting, diarrhea, and
prostration. Dehydration and congestion occur in most internal
organs. Hypertonic salt solutions can produce violent inflammatory
reactions in the gastrointestinal tract.
Sodium Citrate: Extremely large oral dosages may produce
gastrointestinal disturbances.
[Emergency Overview]
WARNING! MAY CAUSE IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT.
WHMIS Symbols
N.A.
[Potential Health Effects]
Sodium Chloride: Poison by intraperitoneal and intracervical routes. Moderately toxic by ingestion, intravenous, and
subcutaneous routes. An experimental teratogen. Human systemic effects by ingestion: blood pressure increase.
Human reproductive effects by intraplacental route: terminates pregnancy. Experimental reproductive effects. Human
mutation data reported. A skin and eye irritant. When bulk sodium chloride is heated to high temperature, a vapor is
emitted that is irritating, particularly to the eyes. Ingestion of large amounts of sodium chloride can cause irritation of
the stomach. Improper use of salt tablets may produce this effect. Potentially explosive reaction with dichloromaleic
anhydride + urea. Electrolysis of mixtures with nitrogen compounds may form the explosive nitrogen trichloride.
Reaction with burning lithium forms the dangerously reactive sodium. The molten salt at 1100掳 reacts explosively
with water. Violent reaction with BrF3. When heated to decomposition it emits toxic fumes of Cl鈭? and Na2O.

Sodium Citrate: Poison by intravenous route. Moderately toxic by intraperitoneal route. When heated to
decomposition it emits toxic fumes of Na2O.
Section 4-First Aid Measures
Skin Contact Get medical aid. Flush skin with plenty of water for at least 15 minutes while removing
contaminated clothing and shoes.
Eye Contact Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower
eyelids. Get medical aid immediately.


EHS-MSDS-20XSSCBuffer pH7.0-9763-01a Page 1 of 4 030806
AMBION, INC. 20X SSC Buffer pH 7.0
Catalog #: 9763, 9765, 9770
Material Safety Data Sheet
Inhalation Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not
breathing, give artificial respiration. If breathing is difficult, give oxygen.
Ingestion Never give anything by mouth to an unconscious person. Do NOT induce vomiting. If conscious
and alert, rinse mouth and drink 2-4 cupfuls of milk or water. Get medical aid if irritation or
symptoms occur.
Section 5-Fire Fighting Measures
Flammable Non flammable
Means of Extinction Use any means suitable for extinguishing surrounding fire.
Flashpoint (oC) and Method Not Tested
Upper Flammable Limit (% by volume) Not Tested
Lower Flammable Limit (% by volume) Not Tested
Autoignition Temperature (oC) Not Tested
Explosion Data 鈥? Sensitivity to Impact Not Tested
Explosion Data 鈥? Sensitivity to Static Discharge Not Tested
Hazardous Combustion Products Not Tested
NFPA Health: 1 Fire: 0 Stability: 0
Section 6-Accidental Release Measures
Leak and Spill Procedures
Ventilate area of leak or spill. Remove all sources of ignition. Wear appropriate personal protective equipment as
specified in Section 8. Isolate hazard area. Keep unnecessary and unprotected personnel from entering. Contain and
recover liquid when possible. Collect liquid in an appropriate container or absorb with an inert material (e. g.,
vermiculite, dry sand, earth), and place in a chemical waste container. Do not use combustible materials, such as
sawdust. Do not flush to sewer!
Section 7-Handling and Storage
Handling Procedures and Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep
Equipment container tightly closed. Avoid ingestion and inhalation.
Storage Requirements Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect
against physical damage. Isolate from incompatible substances.
Section 8-Exposure Control/Personal Protection
Exposure Limits
ACGIH TLV Not Listed
OSHA PEL Not Listed
Other (specify) N.A.
Engineering Controls (specific)
General Facilities storing or utilizing this material should be equipped with an eyewash
facility and a safety shower. Use adequate ventilation to keep airborne
concentrations low.
Local Exhaust A system of local and/or general exhaust is recommended to keep employee
exposures as low as possible. Local exhaust ventilation is generally preferred
because it can control the emissions of the contaminant at its source, preventing
dispersion of it into the general work area. Please refer to the ACGIH document,
Industrial Ventilation, A Manual of Recommended Practices, most recent
edition, for details.
Other N.A.
Personal Protective Equipment (specific)
Gloves Wear impervious gloves.
Respirator A respiratory protection program that meets OSHA's 29 CFR 1910.134 and
ANSI Z88.2 requirements or European Standard EN 149 must be followed
whenever workplace conditions warrant a respirator's use.
Eye Wear appropriate protective eyeglasses or chemical safety goggles as described
by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European
Standard EN166. Maintain eye wash fountain and quick-drench facilities in work
area.
Footwear Wear closed toe shoes.
Clothing Wear appropriate protective clothing to prevent skin exposure.
Other N.A.
Section 9-Physical and Chemical Properties
Physical State Liquid
Odor and Appearance Clear
Odor Threshold (ppm) Not Tested

EHS-MSDS-20XSSCBuffer pH7.0-9763-01a Page 2 of 4 030806
AMBION, INC. 20X SSC Buffer pH 7.0
Catalog #: 9763, 9765, 9770
Material Safety Data Sheet
Specific Gravity Not Tested
Vapor Density (Air=1) Not Tested
Vapor Pressure (mmHg) Not Tested
Evaporation Rate Not Tested
Boiling Point (oC) Not Tested
Freezing Point (oC) Not Tested
pH 7.0
Coefficient of Water/Oil Distribution Not Tested
[Solubility in Water] Soluble
Section 10-Stability and Reactivity
Chemical Stability Stable under ordinary conditions of use and storage. Hygroscopic.
Incompatible with other substances Sodium Chloride: Lithium, Bromine trifluoride.
Sodium Citrate: Strong oxidizers.
Reactivity Not Tested
Hazardous Decomposition Products Sodium Chloride: When heated to above 801C (1474F) it emits
toxic fumes of chloride and sodium oxide.
Sodium Citrate: When heated to decomposition it emits toxic
fumes of Na2O. Carton dioxide and carbon monoxide may form
when heated to decomposition.
Section 11-Toxicological Information
Acute Effects Sodium Chloride: May cause eye irritation. May cause skin irritation. May be
harmful if absorbed through the skin. May be harmful if inhaled. Material may
be irritating to mucous membranes and upper respiratory tract. May be harmful
if swallowed. Ingestion of large amounts causes vomiting and diarrhea.
Sodium Citrate: May be harmful by inhalation, ingestion, or skin absorption.
May cause eye irritation. May cause skin irritation.
Chronic Effects Not Tested
Irritancy of Product Eyes, skin, and respiratory and gastrointestinal system.
Skin Sensitization Not Tested
Respiratory Sensitization Not Tested
Sodium Chloride: LD50 Oral rat: 3000 mg/kg
Sodium Chloride: LC50 Inhalation rat: >42 gm/m3/1H
Sodium Citrate: LD50 IPR-Rat 1548mg/kg
Sodium Citrate: LC50 Not Tested
Carcinogenicity
IARC (1,2A, or 2B) Not Listed
ACGIH (A1, A2, or A3) Not Listed
Reproductive Toxicity Sodium Chloride: Human reproductive effects by intraplacental route:
terminates pregnancy. Experimental reproductive effects.
Teratogenicity Sodium Chloride: An experimental teratogen.
Embryotoxicity Sodium Chloride: Human reproductive effects by intraplacental route:
terminates pregnancy. Experimental reproductive effects.
Mutagenicity Sodium Chloride: Human mutation data reported.
Name of Synergistic Not Listed
Products/Effects
Section 12-Ecological Information
Aquatic Toxicity Not Tested
Section 13-Disposal Considerations
Waste Disposal Chemical waste generators must determine whether a discarded chemical is
classified as a hazardous waste. US EPA guidelines for the classification
determination are listed in 40 CFR Parts 261.3. Additionally, waste generators
must consult state and local hazardous waste regulations to ensure complete and
accurate classification.
Section 14-Transport Information
Special Shipping Information
PIN Not Listed
TDG Not Listed
[DOT] Not Listed
[IMO] Not Listed
[ICAO] Not Listed

EHS-MSDS-20XSSCBuffer pH7.0-9763-01a Page 3 of 4 030806
AMBION, INC. 20X SSC Buffer pH 7.0
Catalog #: 9763, 9765, 9770
Material Safety Data Sheet
Section 15-Regulatory Information
[WHMIS Classification] Not Listed
[OSHA] Not Listed
[SARA] Not Listed
[TSCA] Sodium Chloride is reported in EPA TSCA Inventory.
Sodium Citrate is reported in EPA TSCA Inventory.
R:36/37/38 Irritating to eyes, respiratory system and skin.
S:36/37/39 Wear suitable protective clothing, gloves and eye/face protection.
Section 16-Other Information
This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no
guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are
offered solely for your consideration, investigation, and verification. These suggestions should not be confused with
state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of
these data and information must be determined by the user to be in accordance with applicable federal, state, and local
regulations.




EHS-MSDS-20XSSCBuffer pH7.0-9763-01a Page 4 of 4 030806

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