ISO9001:2000 Certified
Material Safety Data Sheet
Giemsa Stain Solution
Section 1 - Chemical Product and Company Identification
MSDS Name:
Giemsa Stain Solution
Catalog Numbers:
Q04841
Synonyms:
Company Identification:
Qorpak
1195 Washington Pike
Bridgeville, PA 15017
Company Phone Number:
(412) 257-3100
Emergency Phone Number:
(800) 424-9300
CHEMTREC Phone Number:
(800) 424-9300
Section 2 - Composition, Information on Ingredients
CAS# Chemical Name: Percent
51811-82-6 Giemsa Stain 1
67-56-1 Methanol 49.5
56-81-5 Glycerin 49.5
Section 3 - Hazards Identification
Emergency Overview
Appearance: Dark purple solution
Caution! Combustible liquid. May be fatal or cause blindness if swallowed or inhaled. Cannot be
made nontoxic. Causes eye and skin irritation. May cause kidney and liver damage. Contains a
central nervous system depressant.
Target Organs: Eyes, nervous system, optic nerve.
Potential Health Effects
Eye:
Eye contact may result in burning sensation, lachrymation, corneal lesions, vision loss or
impairment, pain or blindness. 5ppm and 40mg of methanol reported to cause moderate irritation.
Skin:
Skin contact can result in defatting, mild dermatitis, and staining of the skin.
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Material Safety Data Sheet
Giemsa Stain Solution
Ingestion:
Inhalation or ingestion causes narcotic intoxication leading to headache, cramps, severe pain,
nausea, diarrhea, fever, and blindness. Kidney and liver damage may occur with possible heart or
respiratory failure to coma and death. Kidney failure can occur in non-fatal cases. Some symptoms
may be delayed for days. Large doses may cause hemolysis, hemoglobinuria, hyperglycemia,
glycosuria, renal failure, convulsions, narcosis, and paralysis.
Inhalation:
Same as for Ingestion above.
Chronic:
Causes blurred or painful vision, conjunctivitis, and possible mutagenic or reproductive effects. May
cause dermatitis.
Section 4 - First Aid Measures
Eyes:
Immediately wash with large amounts of water, lifting upper/lower lids occasionally (15-20min.)
until chemical is gone. Get medical aid at once.
Skin:
Remove contaminated clothing, wash area with soap and water. Flush with large amounts of water
(15-20min.) until chemical is gone. Get medical aid at once.
Ingestion:
GET MEDICAL ATTENTION IMMEDIATELY. Induce vomiting in conscious victim. Give
sodium bicarbonate (baking soda), 2 teaspoons in glass of water.
Inhalation:
Move victim to fresh air, give artificial respiration if necessary. Get medical aid at once.
Section 5 - Fire Fighting Measures
General Information:
As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH
(approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases
may be generated by thermal decomposition or combustion. Use water spray to keep fire-exposed
containers cool. Water may be ineffective. Material is lighter than water and a fire may be spread by
the use of water. Vapors are heavier than air and may travel to a source of ignition and flash back.
Vapors can spread along the ground and collect in low or confined areas.
Extinguishing Media:
For small fires, use dry chemical, carbon dioxide, water spray or alcohol-resistant foam. Water may
be ineffective. For large fires, use water spray, fog or alcohol-resistant foam. Do NOT use straight
streams of water.
Autoignition Temperature:
400掳 (752掳
C F)
Flash Point:
85掳 (29.4掳
F C)
NFPA Rating:
CAS # 51811-82-6: Not published.
CAS # 67-56-1: H- 1, F- 3, I- 0
CAS # 56-81-5: H-1, F- 1, I- 0
Explosion Limits:
Lower: 1.1% Upper: n/a
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Material Safety Data Sheet
Giemsa Stain Solution
Section 6 - Accidental Release Measures
General Information:
Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks:
Contain if possible, and prevent run-off into drains and sewers. Shut off ignition sources, provide
ventilation, and wear respiratory protection. Absorb with sand or other non-combustible material,
scoop into suitable container for disposal.
Section 7 - Handling and Storage
Handling:
Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Ground and
bond containers when transferring material. Use spark-proof tools and explosion proof equipment.
Avoid contact with eyes, skin, and clothing. Empty containers retain product residue, (liquid and/or
vapor), and can be dangerous. Keep container tightly closed. Do not ingest or inhale. Do not
pressurize, cut, weld, braze, solder, drill, grind, or expose empty containers to heat, sparks or open
flames. Use only with adequate ventilation. Keep away from heat, sparks and flame. Avoid use in
confined spaces.
Storage:
Store as heat/ignition protected flammable, away from oxidizers and other incompatibles.
Electrically ground drums when transferring liquid. Do not store in unmarked containers.
Section 8 - Exposure Controls, Personal Protection
Engineering Controls:
Use explosion-proof ventilation equipment. Use adequate general or local exhaust ventilation to
keep airborne concentrations below the permissible exposure limits. An eyewash should be located
in the area where this product is used.
Exposure Limits:
OSHA
NIOSH
ACGIH
Chemical Name:
Giemsa Stain None listed None listed None listed
Methanol 200 ppm TWA; 250 200 ppm TWA; 260 200 ppm TWA; 260
ppm STEL; Skin - mg/m3 TWA 6000 ppm mg/m3 TWA
potential significant IDLH
contribution to overall
exposure by the
cutaneous route
Glycerin 10 mg/m3 TWA None listed 15 mg/m3 TWA (total
dust); 5 mg/m3 TWA
(respirable fraction)
OSHA Vacated PELs:
Methanol: 200 ppm TWA; 260 mg/m3 TWA
Glycerin: 10 mg/m3 TWA (total dust); 5 mg/m3 TWA (respirable fraction)
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Material Safety Data Sheet
Giemsa Stain Solution
Personal Protective Equipment
Eyes:
Wear chemical splash goggles or other approved eyewear.
Skin:
Wear appropriate gloves to prevent skin exposure.
Clothing:
Wear appropriate clothing to prevent skin exposure.
Respirators:
Follow the OSHA respirator regulations found in 29 CFR 1910.134. Always use an NIOSH-
approved respirator when necessary.
Section 9 - Physical and Chemical Properties
Physical State: Liquid
Color: Dark purple
Odor: Slight alcoholic odor
pH: Not available.
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate: Not available.
Viscosity: Not available.
Boiling Point: Not available.
Freezing/Melting Point: Not available.
Decomposition Temperature: Not applicable.
Solubility in water: Soluble
Specific Gravity/Density: 1.05
Molecular Formula: Not applicable.
Molecular Weight: Not applicable.
Section 10 - Stability and Reactivity
Chemical Stability:
Stable under normal temperatures and pressures.
Conditions to Avoid:
High temperatures, ignition sources, confined spaces.
Incompatibilities with Other Materials:
Oxidizing agents, reducing agents, acids, alkali metals, potassium, sodium, metals as powders (e.g.
hafnium, Raney nickel), acid anhydrides, acid chlorides, powdered aluminum, powdered
magnesium, potassium permanganate, caustics (e.g. ammonia, ammonium hydroxide, calcium
hydroxide, potassium hydroxide, sodium hydroxide), isocyanates, aliphatic amines.
Hazardous Decomposition Products:
Carbon monoxide, irritating and toxic fumes and gases, carbon dioxide, formaldehyde.
Hazardous Polymerization:
Will not occur.
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Material Safety Data Sheet
Giemsa Stain Solution
Section 11 - Toxicological Information
RTECS:
CAS# 51811-82-6: unlisted
CAS #67-56-1: PC1400000
CAS# 56-81-5: MA8050000
LD50/LC50:
CAS# 51811-82-6: Not available.
CAS# 67-56-1:
Draize test, rabbit, eye: 40 mg Moderate;
Draize test, rabbit, eye: 100 mg/24H Moderate;
Draize test, rabbit, skin: 20 mg/24H Moderate;
Inhalation, rabbit: LC50 = 81000 mg/m3/14H;
Inhalation, rat: LC50 = 64000 ppm/4H;
Oral, mouse: LD50 = 7300 mg/kg;
Oral, rabbit: LD50 = 14200 mg/kg;
Oral, rat: LD50 = 5600 mg/kg;
Skin, rabbit: LD50 = 15800 mg/kg
CAS# 56-81-5:
Draize test, rabbit, eye: 126 mg Mild;
Draize test, rabbit, eye: 500 mg/24H Mild;
Draize test, rabbit, skin: 500 mg/24H Mild;
Inhalation, rat: LC50 = >570 mg/m3/1H;
Oral, mouse: LD50 = 4090 mg/kg;
Oral, rabbit: LD50 = 27 gm/kg;
Oral, rat: LD50 = 12600 mg/kg;
Skin, rabbit: LD50 = >10 gm/kg
Carcinogenicity:
CAS# 51811-82-6: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 67-56-1: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 56-81-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
Epidemiology:
No information found.
Teratogenicity:
Methanol is considered to be a potential developmental hazard based on animal data. In animal
experiments, methanol has caused fetotoxic or teratogenic effects without maternal toxicity. There is
no human information available.
Reproductive:
See RTECS of individual components.
Mutagenicity:
See RTECS of individual components.
Neurotoxicity:
CAS # 67-56-1: ACGIH cites neuropathy, vision and CNS under TLV basis.
Section 12 - Ecological Information
No information is available for this solution. Based on data from methanol, this product can reasonably
be assumed to be dangerous to aquatic life in high concentrations.
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Material Safety Data Sheet
Giemsa Stain Solution
Section 13 - Disposal Considerations
Dispose of in accordance with Federal, State, and local regulations.
Section 14 - Transport Information
US DOT
Shipping Name: Flammable liquid, n.o.s.
Hazard Class: 3
UN Number: UN1993
Packing Group: PG III
Section 15 - Regulatory Information
US Federal
TSCA:
CAS# 51811-82-6 is listed on the TSCA inventory.
CAS# 67-56-1 is listed on the TSCA inventory.
CAS# 56-81-5 is listed on the TSCA inventory.
SARA Reportable Quantities (RQ):
CAS# 67-56-1: 5000 lb. final RQ; 2270 kg final RQ
CERCLA/SARA Section 313:
This material contains Methanol (CAS# 67-56-1, > 99%), which is subject to the reporting
requirements of Section 313 of SARA Title III and 40 CFR Part 373.
OSHA - Highly Hazardous:
None of the components are on this list.
US State
State Right to Know:
CAS# 51811-82-6 is not present on state lists from California, Pennsylvania, Minnesota,
Massachusetts, Florida, or New Jersey.
CAS# 67-56-1 can be found on the following state right to know lists: California, New Jersey,
Pennsylvania, Minnesota, Massachusetts.
CAS# 56-81-5 can be found on the following state right to know lists: Pennsylvania, Minnesota,
Massachusetts.
California Regulations: None of the chemicals in this product are listed.
European/International Regulations
Canadian DSL/NDSL:
CAS# 51811-82-6 is listed on Canada's DSL List.
CAS# 67-56-1 is listed on Canada's DSL List.
CAS# 56-81-5 is listed on Canada's DSL List.
Canada Ingredient Disclosure List:
CAS# 51811-82-6 is not listed on the Canadian Ingredient Disclosure List.
CAS# 67-56-1 is listed on the Canadian Ingredient Disclosure List.
CAS# 56-81-5 is not listed on the Canadian Ingredient Disclosure List.
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Material Safety Data Sheet
Giemsa Stain Solution
Section 16 - Other Information
MSDS Creation Date: October 25, 2006
Revision Date: None
Information in this MSDS is from available published sources and is believed to be accurate. No warranty,
express or implied, is made and Qorpak assumes no liability resulting from the use of this MSDS. The user
must determine suitability of this information for his application.
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