Material Safety Data Sheet
Revision: 01/23/2006
Isopropanol SDS and Guanidine Guanidine
Hazard information is provided for compliance with both the UK Chemicals
Hydrochloride Hydrochloride,
(Hazard Information and Packaging) (CHIP) Regulations and the US Hazard
Sodium Hydroxide
Communication Standard (HCS) and Isopropanol
IDENTIFICATION OF THE PRODUCT NAME: PRODUCT CODE: EEC NUMBER:
PrepEaseTM MiniSpin Plasmid Kit
SUBSTANCE/PREPARATION 78735 / 78736 / 78737 None
AND COMPANY
SUPPLIER: Emergency Contact:
USB Corporation, 26111 Miles Road, Cleveland, Ohio 44128 Phone: (216) 765-5000 Chemtrec (800) 424-9300
Please visit our website at www.usbweb.com for contact information on USB Outside USA & Canada 703 527 3887
product distributors within your area.
COMPOSITION/
HAZARDOUS COMPONENTS HAZARD CAS NO. %WT TLV CHIP R & S Phrases
Guanidine Hydrochloride 50-01-1 <45% 鈥? R:22 Harmful if swallowed.
(A3 and AW Buffer) R:36/38 Irritating to eyes and skin.
S:23 Do not breathe vapour.
Sodium Dodecyl Sulfate (SDS) 151-21-3 ~1.0% 鈥? R:22 Harmful if swallowed.
(A2 Buffer) R:36/37/38 Irritating to eyes,
respiratory system and skin.
Sodium Hydroxide 1310-73-2 <1% See S:24/25 Avoid contact with skin
(A2 Buffer) 鈥淩egulatory and eyes.
Information鈥? S:26 In case of contact with eyes,
Section rinse immediately with plenty of
water and seek medical advice.
Isopropanol 67-63-0 ~30% See R:10 Flammable.
(AW Buffer) 鈥淩egulatory R:36 Irritating to eyes.
Information鈥? R:67 Vapours may cause
Section drowsiness and dizziness.
S:7 Keep container tightly closed.
S:16 Keep away from sources of
ignition - No smoking.
S:24/25 Avoid contact with skin
and eyes.
S:26 In case of contact with eyes,
rinse immediately with plenty of
water and seek medical advice.
HAZARDS IDENTIFICATION CHIP
Flammable (Isopropanol); Harmful (Guanidine HCl, SDS); Irritant (Isopropanol, Guanidine HCl, Sodium Hydroxide)
HCS
Flammable Liquid (Isopropanol); Toxic (Guanidine HCl); Irritant (Isopropanol, SDS, Sodium Hydroxide)
FIRST-AID MEASURES EYES: Flush with water for 15 minutes. Seek medical advice if irritation persists.
SKIN: Flush with water, then wash thoroughly with soap and water. Remove contaminated clothing and wash
before reuse. Seek medical attention if irritation persists.
INHALATION: Remove the victim from exposure and move to fresh air. If breathing is difficult, give oxygen. If
not breathing, give artificial respiration. Keep victim quiet and warm. Seek immediate medical attention.
INGESTION: Drink water and seek immediate medical attention. Avoid alcoholic beverages. Never give
anything by mouth to an unconscious person.
FIRE-FIGHTING INFORMATION Use media suitable to extinguish the supporting or surrounding fire. Wear NIOSH (or equivalent) approved self
contained breathing apparatus. For small fires only: use carbon dioxide, dry powder or foam. Flammable liquid.
Vapors may travel considerable distance to a source of ignition and flash back. Vapors can spread along the ground
and collect in low or confined areas. Formation of explosive air/vapor mixtures are possible. Emits toxic fumes
under fire conditions. Flash Point = ~24掳C.
ACCIDENTAL RELEASE MEASURES Wear appropriate personal protective equipment and clothing including lab coat, safety goggles, gloves and NIOSH-
approved respirator. Eliminate all sources of ignition. Cover with dry-lime, sand or soda ash. Using non-sparking
tools, collect in a manner that does not create dust and place in a suitable waste container. Avoid contact of
material with skin or eyes. Use adequate ventilation.
HANDLING AND STORAGE Wear appropriate personal protective equipment and clothing including lab coat, safety goggles, gloves and NIOSH-
approved respirator. Avoid heat, sparks and open flame. Keep away from all sources of ignition. Ground all
equipment containing material. Avoid contact of material with skin or eyes. Use adequate ventilation. Store
ambient away from incompatible materials.
MSDS information may be continued on back of page.
PERSONAL PROTECTION Wear appropriate personal protective equipment and clothing including lab coat, safety goggles, gloves and NIOSH-
approved respirator. A qualified industrial hygienist should evaluate the need for respiratory protection. Use
respiratory protection approved by NIOSH (or equivalent) and appropriate to the hazard. Avoid contact of material
with skin or eyes. Mechanical ventilation or local exhaust as needed to control exposure to dust, vapors or mists.
Access to a safety shower and eye-wash.
PHYSICAL AND CHEMICAL Appearance: Kit containing vials of solution (SDS may precipitate) Chemical Formula: Kit
PROPERTIES Solubility (Water): Soluble Specific Gravity: No data available
STABILITY AND REACTIVITY Product is stable. May form peroxides of unknown stability. Avoid excessive heat and acidic conditions.
Hazardous decomposition products include hydrogen chloride gas and oxides of nitrogen, sulfur, sodium and
carbon. Incompatible with strong oxidizing agents, metals, acids, bases, amines, ammonia, ethylene oxide,
isocyanates, chlorine, acid anhydrides, halogens, aluminum and alkalis. Hazardous polymerization will not occur.
TOXICOLOGICAL INFORMATION EFFECTS OF OVEREXPOSURE:
FOR GUANIDINE HYDROCHLORIDE: TARGET ORGAN(S): Bone Marrow and Nerves.
EYES: Vapors may cause irritation or burning upon contact.
SKIN: May be harmful if absorbed through the skin. Causes severe irritation to skin and hypersensitive individuals
may experience an allergic reaction.
INHALATION: May be harmful if inhaled. May cause irritation to mucous membranes and upper respiratory tract.
INGESTION: Harmful if swallowed. Chronic ingestion or excessive dosage may cause gastrointestinal tract
irritation with nausea, vomiting and diarrhea. May cause central nervous system disorders.
FOR ISOPROPANOL: TARGET ORGAN(S): Central Nervous System, Respiratory System, Kidneys, Eyes and Skin.
EYES: Causes irritation characterized by burning, redness, tearing, inflammation and possible corneal injury.
SKIN: May be harmful if absorbed through the skin. Contact causes irritation. Prolonged or repeated contact causes
defatting of skin with irritation, dryness and cracking.
INHALATION: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory
tract. Breathing of vapors may cause drowsiness and dizziness. Inhalation of high concentrations may cause central
nervous system effects characterized by nausea, headache, dizziness, unconsciousness and coma.
INGESTION: May be harmful if swallowed. Aspiration hazard. Aspiration of material into lungs may cause chemical
pneumonitis, which may be fatal. Causes gastrointestinal irritation with nausea, vomiting and diarrhea. May cause
kidney damage. May cause central nervous system depression.
FOR SDS and SODIUM HYDROXIDE:
EYES: Contact may cause severe irritation.
SKIN: Contact may cause irritation. May cause skin sensitization.
INHALATION: May be harmful if inhaled. May cause allergic respiratory reaction. May cause irritation to mucous
membranes and upper respiratory tract.
INGESTION: Harmful if swallowed. Chronic ingestion or excessive dosage may cause irritation of the
gastrointestinal tract with nausea, vomiting and diarrhea.
ADDITIONAL INFORMATION:
Only select RTECS information is provided here. Please see actual RTECS entries for complete information.
Irritation, mutation and toxicity data listed for Guanidine Hydrochloride in RTECS under MF4300000.
Irritation data: Skin Rabbit 500 mg/24H = Severe. Eye Rabbit 81400 ug = Moderate.
Toxicity data: Oral Rat LD50 = 475 mg/kg.
Irritation, reproductive effects, mutation and toxicity data listed for Isopropanol in RTECS under NT8050000.
Irritation data: Skin Rabbit 500 mg = Mild. Eye Rabbit 100 mg/24H= Moderate (1986).
Toxicity data: Oral Rat LD50 5045 mg/kg (1978); Inhalation Rat 16000 ppm/8H (1974). Skin Rabbit
12800 mg/kg (1974).
Carcinogenicity data: ACGIH TLV-Not classifiable as a human carcinogen (2005).
IARC Cancer Review: Animal Inadequate Evidence (1999). Human Inadequate evidence (1999).
Group 3 - Agent is not classifiable as to its carcinogenicity to humans (1999).
Reproductive effects, irritation, mutation and toxicity data for SDS listed in RTECS under WT1050000.
Irritation data: Skin Rabbit 50 mg/24H = Severe (1971). Eye Rabbit 100 mg/24H = Moderate (1972).
Toxicity data: Oral Rat LD50 = 1288 mg/kg (1967). Inhalation Rat LC50 = >3900 mg/m3/1H (1971).
Irritation, mutation and toxicity data for Sodium Hydroxide listed in RTECS under WB4900000.
Irritation data: Skin Rabbit 500 mg/24H = Severe (1972). Eye Rabbit 1 mg/24H = Severe (1964).
Toxicity data: Intraperitoneal Mouse LD50 = 40 mg/kg (1963).
ECOLOGICAL INFORMATION No information available.
DISPOSAL CONSIDERATIONS Dispose of material in accordance with applicable local, state, and federal regulations.
TRANSPORTATION INFORMATION US DOT / IATA: Alcohols, n.o.s. (Isopropanol Solution), UN1987, class 3, PG III, Label: Flammable liquid.
REGULATORY INFORMATION RCRA - No applicable information.
SARA 302 - This material does not have a RQ or TPQ.
SARA 313 - This material contains isopropanol (CAS# 67-63-0, 30%), which is subject to the reporting
requirements of Section 313 of SARA Title III and 40 CFR Part 373.
EPA TSCA Section 8(b) - All listed components: Chemical Inventory.
Exposure Limits - For Isopropanol: ACGIH TLV-TWA 200 ppm; STEL 400 ppm (2005).
OSHA PEL (Gen Indu): 8H TWA 400 ppm (980 mg/cm3) (1994).
NIOSH REL to Isopropyl alcohol-air:10H TWA 400 ppm; STEL 500 ppm.
For Sodium Hydroxide: ACGIH TLV-CL 2 mg/m3. OSHA PEL (Gen Indu): 8H TWA 2 mg/m3.
California Proposition 65 - No applicable information.
This data sheet is based upon information believed to be reliable. The Company makes no statement or warranty as to the accuracy or completeness of the
information contained herein which is offered for your consideration, investigation and verification. Any use of the information contained in this data sheet
must be determined by the user to be in accordance with appropriate applicable regulations.
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