SIGMA-ALDRICH
Material Safety Data Sheet
Date Printed: 30/JUN/2005
Date Updated: 14/MAR/2004
Version 1.2
According to 91/155/EEC
Classified as Hazardous according to the criteria of EU Annex 1 and
NOHSC.
1 - Product and Company Information
Product Name COLCHICINE CRYSTALLINE
Product Number C9754
Company Sigma-Aldrich Pty. Ltd.
12 Anella Avenue
Castle Hill NSW 2154
Technical Phone # +61 2 9841 0555 (1800 800 097)
Fax +61 2 9841 0500 (1800 800 096)
Emergency Phone # +44 8701906777 (1800 448 465)
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
COLCHICINE 64-86-8 200-598-5 614-005-00-6
Formula C22H25NO6
Molecular Weight 399.44 AMU
Synonyms Acetamide,
N-(5,6,7,9-tetrahydro-1,2,3,10-tetramethoxy-9-oxo
benzo(alpha)heptalen-7-yl)- * N-Acetyl
trimethylcolchicinic acid methylether *
Benzo(a)heptalen-9(5H)-one,
7-acetamido-6,7-dihydro-1,2,3,10-tetramethoxy- *
Colchicin (German) * Colchicina (Italian) *
7-alpha-H-Colchicine * Colchineos * Colchisol *
Colcin * Colsaloid * Condylon * NSC 757
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
Very toxic by inhalation and if swallowed.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If not breathing give
artificial respiration. If breathing is difficult, give oxygen.
AFTER SKIN CONTACT
In case of skin contact, flush with copious amounts of water for
at least 15 minutes. Remove contaminated clothing and shoes.
Call a physician.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is
conscious. Call a physician immediately.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Carbon dioxide.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing
to prevent contact with skin and eyes.
6 - Accidental Release Measures
PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid
raising dust. Ventilate area and wash spill site after material
pickup is complete.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Do not breathe dust. Do not get in
eyes, on skin, on clothing. Avoid prolonged or repeated exposure.
STORAGE
Conditions of Storage: Keep tightly closed. Store in a cool dry
place. Store in the dark.
SPECIAL REQUIREMENTS: Light sensitive.
8 - Exposure Controls / Personal Protection
ENGINEERING CONTROLS
Safety shower and eye bath. Use only in a chemical fume hood.
GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Wash thoroughly after
handling.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Government approved respirator.
Hand Protection: Compatible chemical-resistant gloves.
Eye Protection: Chemical safety goggles.
9 - Physical and Chemical Properties
Appearance Physical State: Solid
Color: Faintly beige
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Form: Powder
Property Value At Temperature or Pressure
pH 5.9 Concentration: 5 g/l
BP/BP Range N/A
MP/MP Range 150 藲C
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient N/A
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content <3%
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility Solubility in Water:Soluble.
Other Solvents: METHANOL, CHLOROFORM
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Conditions to Avoid: Light.
Materials to Avoid: Strong oxidizing agents.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide,
and nitrogen oxides.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
11 - Toxicological Information
RTECS NUMBER: GH0700000
ACUTE TOXICITY
LDLO
Oral
Woman
3 mg/kg
Remarks: Behavioral:Convulsions or effect on seizure threshold.
Cardiac:Other changes. Lungs, Thorax, or Respiration:Dyspnea.
LDLO
Oral
Woman
0.6 mg/kg
Remarks: Cardiac:Other changes. Blood:Aplastic anemia.
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Nutritional and Gross Metabolic:Changes in:Metabolic acidosis.
LDLO
Oral
Woman
8.36 mg/kg
Remarks: Cardiac:Other changes. Lungs, Thorax, or
Respiration:Other changes. Kidney, Ureter, Bladder:Changes in
tubules (including acute renal failure, acute tubular necrosis).
LDLO
Oral
Human
0.086 mg/kg
Remarks: Lungs, Thorax, or Respiration:Dyspnea.
Gastrointestinal:Other changes. Nutritional and Gross
Metabolic:Changes in:Body temperature decrease.
LDLO
Oral
Man
11 mg/kg
Remarks: Lungs, Thorax, or Respiration:Respiratory stimulation.
Kidney, Ureter, Bladder:Changes in tubules (including acute
renal failure, acute tubular necrosis). Nutritional and Gross
Metabolic:Changes in:Body temperature increase.
LDLO
Intravenous
Woman
360 UG/KG
6D
Remarks: Kidney, Ureter, Bladder:Other changes. Blood:
Hemorrhage.
LDLO
Intravenous
Man
143 UG/KG
5D-I
Remarks: Blood:Aplastic anemia. Other changes
LDLO
Intravenous
Human
129 UG/KG
Remarks: Vascular:BP lowering not charactertized in autonomic
section. Vascular:Shock. Gastrointestinal:Hypermotility,
diarrhea.
LD50
Intravenous
Rat
1600 UG/KG
Remarks: Behavioral:Somnolence (general depressed activity).
Behavioral:Food intake (animal). Gastrointestinal:Hypermotility,
diarrhea.
LD50
Oral
Mouse
5.886 mg/kg
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LD50
Intraperitoneal
Mouse
1600 UG/KG
LD50
Subcutaneous
Mouse
1200 UG/KG
LD50
Intravenous
Mouse
1700 UG/KG
LD50
Intramuscular
Mouse
1197 UG/KG
Remarks: Nutritional and Gross Metabolic:Weight loss or
decreased weight gain.
LD50
Intravenous
Cat
250 UG/KG
Remarks: Behavioral:Convulsions or effect on seizure threshold.
Gastrointestinal:Nausea or vomiting. Nutritional and Gross
Metabolic:Changes in:Body temperature decrease.
LD50
Intraperitoneal
Guinea pig
500 UG/KG
LD50
Intraperitoneal
Hamster
7300 UG/KG
LD50
Oral
Quail
42 mg/kg
LD50
Intraperitoneal
Gerbil
90 MG/KG
LD50
Oral
Bird (wild)
31.6 mg/kg
IRRITATION DATA
Eyes
Rabbit
1%
3D
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Remarks: Severe irritation effect
SIGNS AND SYMPTOMS OF EXPOSURE
Exposure can cause: Stomach pains, vomiting, diarrhea. To the
best of our knowledge, the chemical, physical, and toxicological
properties have not been thoroughly investigated.
ROUTE OF EXPOSURE
Skin Contact: May cause skin irritation.
Skin Absorption: May be harmful if absorbed through the skin.
Eye Contact: Causes severe eye irritation.
Inhalation: May be fatal if inhaled. Material may be irritating
to mucous membranes and upper respiratory tract.
Ingestion: May be fatal if swallowed.
TARGET ORGAN INFORMATION
Liver. Kidneys. Bone marrow. Nerves. Cardiovascular system.
CHRONIC EXPOSURE - MUTAGEN
Human
50 NMOL/L
Cell Type: lymphocyte
Micronucleus test
Human
20 UG/L
Skin
Micronucleus test
Human
1 MG/L
Cell Type: fibroblast
DNA damage
Human
100 UMOL/L
Cell Type: Other cell types
DNA inhibition
Human
1 MG/L
Cell Type: lymphocyte
DNA inhibition
Human
230 NMOL/L
Cell Type: HeLa cell
DNA inhibition
Human
10 UG/L
Cell Type: lymphocyte
Other mutation test systems
Human
50 NMOL/L
Cell Type: HeLa cell
Cytogenetic analysis
Hamster
100 NMOL/L
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Cell Type: leukocyte
SLN
Human
10 UG/L
Cell Type: fibroblast
Mutation in mammalian somatic cells.
Rat
200 UG/KG
Intraperitoneal
Micronucleus test
Mouse
1250 UG/KG
Intraperitoneal
24H
Micronucleus test
Mouse
200 MG/M3
Inhalation
Micronucleus test
Mouse
8 MG/KG
Oral
Micronucleus test
Mouse
12500 NG/L
Cell Type: Other cell types
Micronucleus test
Mouse
500 UG/L
Cell Type: Other cell types
DNA inhibition
Mouse
350 UG/KG
Intraperitoneal
Other mutation test systems
Mouse
500 NMOL/L
Cell Type: lymphocyte
Other mutation test systems
Mouse
3500 UG/KG
Parenteral
Other mutation test systems
Mouse
10 UMOL/L
Cell Type: Ascites tumor
Other mutation test systems
Mouse
3330 UG/KG
Cell Type: Ascites tumor
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Other mutation test systems
Mouse
40 MG/KG
Intraperitoneal
Other mutation test systems
Mouse
4 UMOL/L
Cell Type: Ascites tumor
Cytogenetic analysis
Mouse
200 UG/KG
Intraperitoneal
SLN
Mouse
666 PPM
Cell Type: Embryo
Mutation in mammalian somatic cells.
Mouse
3 MG/KG
Intraperitoneal
5D
sperm
Mouse
3500 UG/KG
Parenteral
sperm
Hamster
100 UMOL/L
Cell Type: Embryo
Micronucleus test
Hamster
2500 UG/KG
Intraperitoneal
Micronucleus test
Hamster
10 UG/L
Cell Type: lung
Micronucleus test
Hamster
1600 MG/L
Cell Type: ovary
Cytogenetic analysis
Hamster
1 MG/L
10M
Cell Type: fibroblast
Cytogenetic analysis
Hamster
3 UG/KG
Intraperitoneal
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SLN
Hamster
6250 UG/L
Cell Type: Embryo
SLN
Hamster
62500 UG/L
Cell Type: ovary
SLN
Cattle, Horse
20 UG/L
Skin
Micronucleus test
Cattle, Horse
20 UG/L
Cell Type: Other cell types
Micronucleus test
Rabbit
30 UMOL/L
Cell Type: kidney
DNA inhibition
CHRONIC EXPOSURE - TERATOGEN
Result: Laboratory experiments have shown teratogenic effects.
Species: Rat
Dose: 1200 UG/KG
Route of Application: Subcutaneous
Exposure Time: (18-20D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system. Effects on Newborn: Growth statistics (e.g., reduced
weight gain). Effects on Newborn: Behavioral.
Species: Rat
Dose: 1200 UG/KG
Route of Application: Subcutaneous
Exposure Time: (18-20D PREG)
Result: Effects on Embryo or Fetus: Cytological changes
(including somatic cell genetic material).
Species: Mouse
Dose: 500 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (7D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system. Specific Developmental Abnormalities: Eye, ear. Specific
Developmental Abnormalities: Body wall.
Species: Mouse
Dose: 500 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (7D PREG)
Result: Specific Developmental Abnormalities: Urogenital system.
Species: Mouse
Dose: 1 MG/KG
Route of Application: Intraperitoneal
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Exposure Time: (8D PREG)
Result: Effects on Embryo or Fetus: Cytological changes
(including somatic cell genetic material).
Species: Mouse
Dose: 500 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (8D PREG)
Result: Specific Developmental Abnormalities: Musculoskeletal
system. Effects on Embryo or Fetus: Fetal death.
Species: Mouse
Dose: 2 MG/KG
Route of Application: Subcutaneous
Exposure Time: (7-10D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system. Specific Developmental Abnormalities: Body wall.
Specific Developmental Abnormalities: Musculoskeletal system.
Species: Mouse
Dose: 1250 UG/KG
Route of Application: Subcutaneous
Exposure Time: (9D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system. Specific Developmental Abnormalities: Eye, ear. Specific
Developmental Abnormalities: Craniofacial (including nose and
tongue).
Species: Mouse
Dose: 3750 UG/KG
Route of Application: Subcutaneous
Exposure Time: (4-6D PREG)
Result: Effects on Embryo or Fetus: Fetal death. Specific
Developmental Abnormalities: Eye, ear.
Species: Mouse
Dose: 1 MG/KG
Route of Application: Subcutaneous
Exposure Time: (9D PREG)
Result: Effects on Embryo or Fetus: Fetal death.
Species: Hamster
Dose: 10 MG/KG
Route of Application: Intravenous
Exposure Time: (8D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system. Specific Developmental Abnormalities: Eye, ear. Specific
Developmental Abnormalities: Body wall.
Species: Hamster
Dose: 10 MG/KG
Route of Application: Intravenous
Exposure Time: (8D PREG)
Result: Effects on Embryo or Fetus: Fetal death. Specific
Developmental Abnormalities: Musculoskeletal system.
CHRONIC EXPOSURE - REPRODUCTIVE HAZARD
Species: Rat
Dose: 250 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (1D MALE)
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Result: Paternal Effects: Spermatogenesis (including genetic
material, sperm morphology,motility, and count).
Species: Rat
Dose: 5 MG/KG
Route of Application: Subcutaneous
Exposure Time: (8D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Species: Mouse
Dose: 80 MG/KG
Route of Application: Oral
Exposure Time: (8-12D PREG)
Result: Effects on Newborn: Live birth index (# fetuses per
litter; measured after birth). Effects on Newborn: Viability
index (e.g., # alive at day 4 per # born alive).
Species: Mouse
Dose: 2500 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (1D MALE)
Result: Effects on Fertility: Pre-implantation mortality (e.g.,
reduction in number of implants per female; total number of
implants per corpora lutea).
Species: Mouse
Dose: 320 MG/KG
Route of Application: Intratesticular
Exposure Time: (2D MALE)
Result: Paternal Effects: Spermatogenesis (including genetic
material, sperm morphology,motility, and count).
Species: Rabbit
Dose: 22500 UG/KG
Route of Application: Subcutaneous
Exposure Time: (30D MALE)
Result: Paternal Effects: Testes, epididymis, sperm duct.
Species: Rabbit
Dose: 4 MG/KG
Route of Application: Intravenous
Exposure Time: (1D PRE)
Result: Effects on Fertility: Other measures of fertility
Species: Domestic Animals
Dose: 1 MG/KG
Route of Application: Intravenous
Exposure Time: (1D PRE)
Result: Maternal Effects: Other effects.
Species: Cattle, Horse
Dose: 2 UG/KG
Route of Application: Intrauterine
Exposure Time: (42D PREG)
Result: Maternal Effects: Other effects. Effects on Fertility:
Post-implantation mortality (e.g., dead and/or resorbed implants
per total number of implants).
12 - Ecological Information
No data available.
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13 - Disposal Considerations
SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose
of this material. Dissolve or mix the material with a combustible
solvent and burn in a chemical incinerator equipped with an
afterburner and scrubber. Observe all federal, state, and local
environmental regulations.
14 - Transport Information
RID/ADR
UN#: 1544
Class: 6.1
PG: I
Proper Shipping Name: Alkaloids, solid, n.o.s.
IMDG
UN#: 1544
Class: 6.1
PG: I
Proper Shipping Name: Alkaloids, solid, n.o.s.
Marine Pollutant: No
Severe Marine Pollutant: No
Technical Name: Required
IATA
UN#: 1544
Class: 6.1
PG: I
Proper Shipping Name: Alkaloids, solid, n.o.s.
Inhalation Packing Group I: No
Technical Name: Required
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
ANNEX I INDEX NUMBER: 614-005-00-6
INDICATION OF DANGER: T+
Very toxic.
R-PHRASES: 26/28
Very toxic by inhalation and if swallowed.
S-PHRASES: 13 45
Keep away from food, drink, and animal feedingstuffs. In case
of accident or if you feel unwell, seek medical advice
immediately (show the label where possible).
COUNTRY SPECIFIC INFORMATION
Germany
WGK: 3
SWITZERLAND
SWISS POISON CLASS: 1*
16 - Other Information
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
SIGMA - C9754 www.sigma-aldrich.com Page 12
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2005 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
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