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MSDS 
:
 Nitro Blue tetrazolium
CAS 
:
 298-83-9
SYNONYMS 
:
 NBT (dye); Nitro Blue tetrazolium chloride; Nitro BT; p-Nitro Blue     tetrazolium; p-Nitro Blue tetrazolium chloride.
**** SECTION 2 - COMPOSITION, INFORMATION ON INGREDIENTS ****

+----------------+--------------------------------------+----------+-----------+
| CAS# | Chemical Name | % | EINECS# |
|----------------|--------------------------------------|----------|-----------|
| 298-83-9 |Nitro BT | app. 100 | 206-067-4 |
+----------------+--------------------------------------+----------+-----------+
Hazard Symbols: XN
Risk Phrases: 21/22

**** SECTION 3 - HAZARDS IDENTIFICATION ****

EMERGENCY OVERVIEW
Harmful in contact with skin and if swallowed.The toxicological
properties of this material have not been fully investigated.

Potential Health Effects
Eye:
Causes eye irritation. Contact may cause transient eye irritation.
Skin:
Causes skin irritation.
Ingestion:
Causes gastrointestinal irritation with nausea, vomiting and
diarrhea. The toxicological properties of this substance have not
been fully investigated.
Inhalation:
Causes respiratory tract irritation. The toxicological properties of
this substance have not been fully investigated.
Chronic:
No information found.

**** SECTION 4 - FIRST AID MEASURES ****

Eyes:
Flush eyes with plenty of water for at least 15 minutes,
occasionally lifting the upper and lower eyelids. Get medical aid.
Skin:
Flush skin with plenty of water for at least 15 minutes while
removing contaminated clothing and shoes. Get medical aid if
irritation develops or persists. Wash clothing before reuse.
Ingestion:
Never give anything by mouth to an unconscious person. Get medical
aid. Do NOT induce vomiting. If conscious and alert, rinse mouth and
drink 2-4 cupfuls of milk or water.
Inhalation:
Remove from exposure and move to fresh air immediately. If not
breathing, give artificial respiration. If breathing is difficult,
give oxygen. Get medical aid if cough or other symptoms appear.
Notes to Physician:
Treat symptomatically and supportively.

**** SECTION 5 - FIRE FIGHTING MEASURES ****

General Information:
As in any fire, wear a self-contained breathing apparatus in
pressure-demand, MSHA/NIOSH (approved or equivalent), and full
protective gear. Combustion generates toxic fumes. During a fire,
irritating and highly toxic gases may be generated by thermal
decomposition or combustion. Vapors may be heavier than air. They
can spread along the ground and collect in low or confined areas.
Extinguishing Media:
Use water spray, dry chemical, or carbon dioxide. Use water spray,
dry chemical, carbon dioxide, or appropriate foam.

**** SECTION 6 - ACCIDENTAL RELEASE MEASURES ****

General Information: Use proper personal protective equipment as indicated
in Section 8.
Spills/Leaks:
Clean up spills immediately, observing precautions in the Protective
Equipment section. Sweep up, then place into a suitable container for
disposal. Avoid generating dusty conditions. Provide ventilation.

**** SECTION 7 - HANDLING and STORAGE ****

Handling:
Wash thoroughly after handling. Remove contaminated clothing and
wash before reuse. Use with adequate ventilation. Minimize dust
generation and accumulation. Avoid contact with skin and eyes. Keep
container tightly closed. Avoid ingestion and inhalation.
Storage:
Store in a tightly closed container. Keep containers tightly closed.
Store at -20C.

**** SECTION 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION ****

Engineering Controls:
Facilities storing or utilizing this material should be equipped
with an eyewash facility and a safety shower. Use adequate
ventilation to keep airborne concentrations low.
Exposure Limits
CAS# 298-83-9:


Personal Protective Equipment

Eyes:
Wear appropriate protective eyeglasses or chemical
safety goggles as described by OSHA's eye and face
protection regulations in 29 CFR 1910.133 or European
Standard EN166.
Skin:
Wear appropriate gloves to prevent skin exposure.
Clothing:
Wear appropriate clothing to prevent skin exposure.
Respirators:
A respiratory protection program that meets OSHA's 29
CFR 1910.134 and ANSI Z88.2 requirements or European
Standard EN 149 must be followed whenever workplace
conditions warrant respirator use.

**** SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES ****

Physical State: Solid
Color: yellow
Odor: none reported
pH: Not available.
Vapor Pressure: Negligible.
Viscosity: Not available.
Boiling Point: Not available.
Freezing/Melting Point: 189 deg C
Autoignition Temperature: Not applicable.
Flash Point: Not applicable.
Explosion Limits, lower: Not available.
Explosion Limits, upper: Not available.
Decomposition Temperature: > 189 deg C
Solubility in water: Not available.
Specific Gravity/Density: Not available.
Molecular Formula: C40H30N10O6Cl2
Molecular Weight: 817.70

**** SECTION 10 - STABILITY AND REACTIVITY ****

Chemical Stability:
Stable. However, may decompose if heated. Stable at room temperature
in closed containers under normal storage and handling conditions.
Conditions to Avoid:
Incompatible materials, excess heat, temperatures above 50C (122F).
Incompatibilities with Other Materials:
Strong oxidizing agents.
Hazardous Decomposition Products:
Hydrogen chloride, carbon monoxide, oxides of nitrogen, irritating
and toxic fumes and gases, carbon dioxide.
Hazardous Polymerization: Has not been reported.

**** SECTION 11 - TOXICOLOGICAL INFORMATION ****

RTECS#:
CAS# 298-83-9: XF8045000
LD50/LC50:
CAS# 298-83-9: Oral, mouse: LD50 = 2 gm/kg.
Carcinogenicity:
Nitro BT -
Not listed by ACGIH, IARC, or NTP.
Other:
See actual entry in RTECS for complete information.

**** SECTION 12 - ECOLOGICAL INFORMATION ****

Other
No information available.

**** SECTION 13 - DISPOSAL CONSIDERATIONS ****

Products which are considered hazardous for supply are classified as Special
Waste and the disposal of such chemicals is covered by regulations which may
vary according to location. Contact a specialist disposal company or the local
waste regulator for advice. Empty containers must be decontaminated before
returning for recycling.

**** SECTION 14 - TRANSPORT INFORMATION ****

IATA
Shipping Name: TOXIC SOLID, ORGANIC NOS
Hazard Class: 6.1
UN Number: 2811
Packing Group: II
IMO
Shipping Name: TOXIC SOLID, ORGANIC NOS
Hazard Class: 6.1
UN Number: 2811
Packing Group: II
RID/ADR
Shipping Name: TOXIC SOLID, ORGANIC NOS
Hazard Class: 6.1
UN Number: 2811
Packing group: II

**** SECTION 15 - REGULATORY INFORMATION ****

European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols: XN
Risk Phrases:
R 21/22 Harmful in contact with skin and if
swallowed.
Safety Phrases:
S 22 Do not breathe dust.
S 25 Avoid contact with eyes.
WGK (Water Danger/Protection)
CAS# 298-83-9: No information available.
Canada
CAS# 298-83-9 is listed on Canada's DSL List.
CAS# 298-83-9 is not listed on Canada's Ingredient Disclosure List.
US FEDERAL
TSCA
CAS# 298-83-9 is listed on the TSCA inventory.

**** SECTION 16 - ADDITIONAL INFORMATION ****

MSDS Creation Date: 8/07/1998 Revision #6 Date: 2/20/2004

The information above is believed to be accurate and represents the best
information currently available to us. However, we make no warranty of
merchantability or any other warranty, express or implied, with respect to
such information, and we assume no liability resulting from its use. Users
should make their own investigations to determine the suitability of the
information for their particular purposes. In no way shall the company be
liable for any claims, losses, or damages of any third party or for lost
profits or any special, indirect, incidental, consequential or exemplary
damages, howsoever arising, even if the company has been advised of
the possibility of such damages.
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