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MSDS 
:
 Ethidium bromide solution
CAS 
:
 1239-45-8
SYNONYMS 
:
 None
**** SECTION 2 - COMPOSITION, INFORMATION ON INGREDIENTS ****

+----------------+--------------------------------------+----------+-----------+
| CAS# | Chemical Name | % | EINECS# |
|----------------|--------------------------------------|----------|-----------|
|----------------|--------------------------------------|----------|-----------|
| 1239-45-8 |Ethidium bromide | 1 | 214-984-6 |
|----------------|--------------------------------------|----------|-----------|
| 7732-18-5 |Water | 99 | 231-791-2 |
+----------------+--------------------------------------+----------+-----------+
Text for R-phrases: see Section 16
Hazard Symbols: XN
Risk Phrases: 20

**** SECTION 3 - HAZARDS IDENTIFICATION ****

EMERGENCY OVERVIEW
Harmful by inhalation.

Potential Health Effects
Eye:
May cause eye irritation.
Skin:
May cause skin irritation.
Ingestion:
May cause gastrointestinal irritation with nausea, vomiting and
diarrhea.
Inhalation:
May cause respiratory tract irritation. Methemoglobinemia is
characterized by dizziness, drowsiness, headache, shortness of
breath, cyanosis (bluish discoloration of skin due to deficient
oxygenation of the blood), rapid heart rate and chocolate-brown
blood. Harmful if inhaled. May cause methemoglobinemia.
Chronic:
May cause methemoglobinemia, which is characterized by
chocolate-brown colored blood, headache, weakness, dizziness, breath
shortness, cyanosis (bluish skin due to deficient oxygenation of
blood), rapid heart rate, unconsciousness and possible death. May
alter genetic material.

**** SECTION 4 - FIRST AID MEASURES ****

Eyes:
In case of contact, immediately flush eyes with plenty of water for
at least 15 minutes. Get medical aid.
Skin:
In case of contact, immediately flush skin with plenty of water.
Remove contaminated clothing and shoes. Get medical aid. Wash
clothing before reuse.
Ingestion:
If swallowed, do not induce vomiting unless directed to do so by
medical personnel. Never give anything by mouth to an unconscious
person. Get medical aid.
Inhalation:
If inhaled, remove to fresh air. If not breathing, give artificial
respiration. If breathing is difficult, give oxygen. Get medical aid.
Notes to Physician:
Treat symptomatically and supportively.

**** SECTION 5 - FIRE FIGHTING MEASURES ****

General Information:
As in any fire, wear a self-contained breathing apparatus in
pressure-demand, MSHA/NIOSH (approved or equivalent), and full
protective gear. During a fire, irritating and highly toxic gases
may be generated by thermal decomposition or combustion.
Extinguishing Media:
Substance is nonflammable; use agent most appropriate to extinguish
surrounding fire.

**** SECTION 6 - ACCIDENTAL RELEASE MEASURES ****

General Information: Use proper personal protective equipment as indicated
in Section 8.
Spills/Leaks:
Absorb spill with inert material (e.g. vermiculite, sand or earth),
then place in suitable container. Avoid runoff into storm sewers and
ditches which lead to waterways. Clean up spills immediately,
observing precautions in the Protective Equipment section. Provide
ventilation.

**** SECTION 7 - HANDLING and STORAGE ****

Handling:
Wash thoroughly after handling. Remove contaminated clothing and
wash before reuse. Avoid contact with eyes, skin, and clothing. Keep
container tightly closed. Avoid ingestion and inhalation. Use only
with adequate ventilation or respiratory protection.
Storage:
Keep container closed when not in use. Store in a cool, dry,
well-ventilated area away from incompatible substances.

**** SECTION 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION ****

Engineering Controls:
Facilities storing or utilizing this material should be equipped
with an eyewash facility and a safety shower. Use adequate
ventilation to keep airborne concentrations low.
Exposure Limits
CAS# 1239-45-8:

CAS# 7732-18-5:


Personal Protective Equipment

Eyes:
Wear appropriate protective eyeglasses or chemical
safety goggles as described by OSHA's eye and face
protection regulations in 29 CFR 1910.133 or European
Standard EN166.
Skin:
Wear appropriate gloves to prevent skin exposure.
Clothing:
Wear appropriate protective clothing to prevent skin
exposure.
Respirators:
Follow the OSHA respirator regulations found in 29
CFR 1910.134 or European Standard EN 149. Use a
NIOSH/MSHA or European Standard EN 149 approved
respirator if exposure limits are exceeded or if
irritation or other symptoms are experienced.

**** SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES ****

Physical State: Liquid
Color: red-orange
Odor: no odor
pH: Not available.
Vapor Pressure: Not available.
Viscosity: Not available.
Boiling Point: Not available.
Freezing/Melting Point: Not available.
Autoignition Temperature: Not available.
Flash Point: Not available.
Explosion Limits, lower: Not available.
Explosion Limits, upper: Not available.
Decomposition Temperature:
Solubility in water:
Specific Gravity/Density:
Molecular Formula: Solution
Molecular Weight: 0

**** SECTION 10 - STABILITY AND REACTIVITY ****

Chemical Stability:
Stable under normal temperatures and pressures.
Conditions to Avoid:
Excess heat.
Incompatibilities with Other Materials:
Strong oxidizing agents.
Hazardous Decomposition Products:
Nitrogen oxides, carbon monoxide, carbon dioxide, hydrogen bromide.
Hazardous Polymerization: Will not occur.

**** SECTION 11 - TOXICOLOGICAL INFORMATION ****

RTECS#:
CAS# 1239-45-8: SF7950000
CAS# 7732-18-5: ZC0110000
LD50/LC50:
Not available.
CAS# 7732-18-5: Oral, rat: LD50 = >90 mL/kg.
Ethidium Bromide:
mg/L/6H.
toxic based on: LC50 (inhalation rat) 0.0472mg/L/1H * 100 (dilution
rate)=approximately 4.72mg/L/1H.
Carcinogenicity:
Ethidium bromide -
Not listed by ACGIH, IARC, or NTP.
Water -
Not listed by ACGIH, IARC, or NTP.
Other:
See actual entry in RTECS for complete information.

**** SECTION 12 - ECOLOGICAL INFORMATION ****



**** SECTION 13 - DISPOSAL CONSIDERATIONS ****

Products which are considered hazardous for supply are classified as Special
Waste and the disposal of such chemicals is covered by regulations which may
vary according to location. Contact a specialist disposal company or the local
waste regulator for advice. Empty containers must be decontaminated before
returning for recycling.

**** SECTION 14 - TRANSPORT INFORMATION ****

IATA
Shipping Name: TOXIC LIQUIDS, ORGANIC, N.O.S.
Hazard Class: 6.1
UN Number: 2810
Packing Group: III
IMO
Shipping Name: TOXIC LIQUIDS, ORGANIC, N.O.S.
Hazard Class: 6.1
UN Number: 2810
Packing Group: III
RID/ADR
Shipping Name: TOXIC LIQUIDS, ORGANIC, N.O.S.
Hazard Class: 6.1
UN Number: 2810
Packing group: III

**** SECTION 15 - REGULATORY INFORMATION ****

European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols: XN
Risk Phrases:
R 20 Harmful by inhalation.
Safety Phrases:
S 36/37/39 Wear suitable protective clothing, gloves
and eye/face protection.
WGK (Water Danger/Protection)
CAS# 1239-45-8: 2
CAS# 7732-18-5: No information available.
Canada
CAS# 7732-18-5 is listed on Canada's DSL List.
CAS# 1239-45-8 is not listed on Canada's Ingredient Disclosure List.
CAS# 7732-18-5 is not listed on Canada's Ingredient Disclosure List.
US FEDERAL
TSCA
CAS# 1239-45-8 is not listed on the TSCA inventory.
It is for research and development use only.
CAS# 7732-18-5 is listed on the TSCA inventory.

**** SECTION 16 - ADDITIONAL INFORMATION ****

Text for R-phrases from Section 2
MSDS Creation Date: 4/08/1999 Revision #9 Date: 6/25/2004

The information above is believed to be accurate and represents the best
information currently available to us. However, we make no warranty of
merchantability or any other warranty, express or implied, with respect to
such information, and we assume no liability resulting from its use. Users
should make their own investigations to determine the suitability of the
information for their particular purposes. In no way shall the company be
liable for any claims, losses, or damages of any third party or for lost
profits or any special, indirect, incidental, consequential or exemplary
damages, howsoever arising, even if the company has been advised of
the possibility of such damages.
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