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Material Safety Data Sheet
DAB-Nickel peroxidase kit
1: Chemical Product and Company Identification
Product Name: DAB-Nickel peroxidase kit Contact information:
Catalog Code: 750090 bioWORLD
4150 Tuller Rd
CAS#: Mixture. Suite 228, Dublin, OH 43017, USA
RTECS: Mixture. For information, call: 614-792-8680
Fax Number: 614-792-8685
TSCA: TSCA 8(b) inventory : Components were listed. Order Online:www.bio-world.com
CI#: Not available. E-mail : save@bio-world.com
In case of a chemical emergency, contact local
Synonym: Not available.
hospital.
Chemical Name: DAB-Nickel peroxidase kit
For non-emergency assistance,call: 1-800-860-9729
Chemical Formula: Mixture.
2: Composition and Information on Ingredients
Composition:
Name CAS# % by Weight
DAB substrate 91-95-2
Peroxidase solution 7722-84-1
-
Nickel chloride 7718-54-9
Cobalt chloride 7791-13-1
Toxicological Data on Ingredients:
3,3 Diaminobenzidine
LD50 (Oral, Mouse): 1834 mg/kg
LD50 (Oral,Rat): 4700 mg/kg
3: Hazards Identification
Potential Acute Health Effects: Harmful if inhaled, absorbed through skin or swallowed. Causes respiratory tract, eye
and skin irritation. May cause allergic respiratory and skin reaction. Suspect cancer hazard Contains material which may
cause cancer Flammable liquid and vapor. Vapor may cause flash fire.
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Potential Chronic Health Effects:
CARCINOGENIC EFFECTS: Suspected carcinogen.
MUTAGENIC EFFECTS: Not available.
TERATOGENIC EFFECTS: Not available.
DEVELOPMENTAL TOXICITY: Not available.
4: First Aid Measures
Eye Contact: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes.
Skin Contact: In case of contact, immediately wash skin with soap and copious amounts of water.
Serious Skin Contact: Wash with a disinfectant soap and cover the contaminated skin with an anti-bacterial cream. Seek
medical attention.
Inhalation: If inhaled, remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give oxygen.
Serious Inhalation: Not available.
Ingestion: If swallowed, wash out mouth with water provided person is conscious. Call a physician.
Serious Ingestion: Not available.
5: Fire and Explosion Data
Flammability of the Product: Flammable.
Auto-Ignition Temperature: Not available.
Flash Points: <30掳C (86掳F). (Closed cup)
Flammable Limits: Not available.
Product of Combustion: Carbon oxides, nitrogen oxides, halogen oxides.
Fire Hazards in Presence of Various Substances: Extremely flammable in presence of open flames, sparks and static
discharge. Highly flammable in presence of heat.
Explosion Hazards in Presence of Various Substances:
Not available.
Fire Fighting Media and Instructions:
SMALL FIRE: Dry chemical powder.
LARGE FIRE: Water spray. Carbon dioxide, dry chemical powder, or appropriate foam.
Special Remarks on Fire Hazards: Not available.
Special Remarks on Explosion Hazards: Not available.
6: Accidental Release Measures
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Small Spill: Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal
container. If necessary: Neutralize the residue with a dilute solution of sodium carbonate.
Large Spill: Use a shovel to put the material into a convenient waste disposal container. Finish cleaning by spreading
water on the contaminated surface and allow to evacuate through the sanitary system.
7: Handling and Storage
Precautions: Do not ingest. Avoid contact with eyes, skin and clothing. Keep container closed. Use only with adequate
ventilation. Do not breathe vapor or mist. Avoid contact of spilled material and runoff with soil and surface waterways. Wash
thoroughly after handling.
Storage: Keep container tightly closed. Keep container in a cool, well-ventilated area.
8: Exposure Controls/Personal Protection
Engineering Controls: Safety shower and eye bath. Mechanical exhaust required.
Personal Protection: Respiratory : Government approved respirator.
Hand: Chemical resistant gloves.
Eye: Safety glasses.
Personal Protection in Case of a Large Spill: Splash goggles. Full suit. Boots. Dust respirator. Gloves.
Exposure Limits: Cobalt Chloride ACGIH
TWA: 0.02 mg/m3
Nickel Chloride ACGIH
TWA: 0.1 mg/m3
9: Physical and Chemical Properties
Physical State and Appearance: Liquid.
Odor: Not available.
Taste: Not available.
Molecular Weight: Not available.
Color: Not available.
pH(1% soln/water): 1.5 - 2
Boiling Point: Not available.
Melting Point: Not available.
Critical Temperature: Not available.
Specific Gravity: Not available.
Vapour Pressure: Not available.
Vapor Density: Not available.
Volatility: Not available.
Odor Threshold: Not available.
Water/Oil Dist. Coeff: Not available.
Ionicity (in Water): Not available.
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Dispersion Properties: Not available.
Solubility: Easily soluble in cold water, hot water, methanol, diethyl ether. Partially soluble in acetone. Very slightly soluble in
n-octanol.
10: Stability and Reactivity Data
Stability: Stable.
Instability Temperature: Not available.
Conditions of Instability: Not available.
Incompatibility with various substances: Oxidizing agents, reducing agents, metals, acids, alkalis.
Corrosivity: Not available.
Special Remarks on Reactivity: Not available.
Special Remarks on Corrosivity: Not available.
Polymerization: Will not occur.
11:Toxicological Information
Routes of Entry: Skin contact. Eye contact. Ingestion. Inhalation.
Toxicity to Animals:
3,3 Diaminobenzidine
LD50 (Oral, Mouse): 1834 mg/kg
LD50 (Oral,Rat): 4700 mg/kg
Chronic Effects on Humans: Carcinogen: Suspected carcinogen.
Other Toxic Effects on Humans: Skin Contact: May cause skin irritation.
Skin Absorption: May be harmful if absorbed through the skin.
Eye Contact: May cause eye irritation.
Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract.
Ingestion: May be harmful if swallowed.
Special Remarks on Toxicity to Animals: Not available.
Special Remarks on Chronic Effects on Humans: Not available.
Special Remarks on other Toxic Effects on Humans: Not available.
12: Ecological Information
Ecotoxicity: Not available.
BOD5 and COD: Not available.
Product of Biodegradation: Not available.
Toxicity of the Products of Biodegradation: Not available.
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Special Remarks on the Products of Biodegradation: Not available.
13: Disposal Considerations
Waste Disposal: Waste must be disposed of in accordance with federal, state and local environmental control regulations.
14: Transport Information
DOT Classification: Not a DOT controlled material.
Identification: Not available.
Special Provisions for Transport: Not available.
15: Other Regulatory Information
Federal and State Regulations: TSCA 8(b) inventory: 3,3'-Diaminobenzidine; Hydrogen peroxide; Nickel chloride; Cobalt
chloride.
Other Regulations: R49- May cause cancer by inhalation.
R23/24/25- Toxic by inhalation, in contact with skin and if swallowed.
R39/23/24/25- Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowed.
R42/43- May cause sensitization by inhalation and skin contact.
R52/53- Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
Other Classifications:
WHMIS(Canada): Not available.
DSCL(EEC): Not available.
HMIS(U.S.A):
Health Hazard: 2
Fire Hazard: 3
Reactivity: 0
Personal Protection:
National Fire Protection Association (U.S.A):
Health: 2
Flammability: 3
Reactivity: 0
Specific hazard:
Protective Equipment: Gloves. Lab coat. Dust respirator. Be sure to use an approved/certified respirator or equivalent.
Safety goggles.
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16: Other Information
References: Not available.
Other Special Considerations: Not available.
Created: 12/12/2006 11:54:28 AM
Last Updated: 12/12/2006 2:55:06 PM
Disclaimer
This product is offered by bioWORLD for research, laboratory or further manufacturing use. Not for human use or
consumption.
The information above is believed to be accurate and represents the best information currently available to us. However,we
make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we
assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the
information for their particular purposes.In no event shall Bio-World.com be liable for any claims, losses, or damages of any
third party or for lost profits or any special, indirect,incidental,consequential or exemplary damages, howsoever arising,
even if Bio-World.com has been advised of the possibility of such damages.
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