庐
Biomolecules for research MATERIAL SAFETY DATA SHEET
Section 1 : Substance Identification
SK-Mel-28 InstaCell Proliferation Assay with XTT
Name:
Catalog Number: CE-105
Company Identification: BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting PA 19462-1202 USA
(610) 941-0430 (9am-5pm ET)
Section 2 : Components
Component # Name: Contents: Quantity
KI-322 Multiwell plate with SK-Mel-28 human skin carcinoma 4 plates
frozen SK-Mel-28 cells cells frozen in culture media
with 10% fetal calf serum
The SK-Mel-28 cell line has been
well characterized, and has not been
genetically modified.
KI-313 Culture Medium Aqueous nutrient solution 50 ml
KI-329 Dilution Buffer Aqueous buffer containing PMS 50 ml
(0.01 mg/ml)
CAS#: 299-11-6
Formula: C13H11N2 路 CH3SO4
MW= 306.3
Synonyms: 5-Methylphenazinum
methyl sulfate; Phenazine
methosulfate
KI-321 XTT Solution XTT sodium salt 25 ml
1 mg/ml in aqueous buffer
CAS#: 111072-31-2
Formula: C22H16N7O13S2Na
MW= 673.5
Synonyms: 2,3-bis(2-methoxy-4-nitro-
5-sulfophenyl)-5-[(phenylamino)
carbonyl]-2H-tetrazolium sodium
salt
KI-316 5-Fluorouracil 5 mM solution in DMSO 2 x 1.8 ml
(positive control) CAS#: 51-21-8
Formula: C4H3FN2O2
MW= 130.1
Synonyms: 2,4-Dihydroxy-5-fluoro
pyrimidine, 5-FU
CE-105 Instruction Booklet 1 booklet
Section 3 : Health Hazards
Please be advised that the physical, chemical and toxicological properties of many of these components
have not yet been fully investigated. It is therefore advised that all laboratory personnel handling this
product follow standard laboratory safety procedures. It is recommended that personnel wear safety
goggles, gloves, and protective clothing at all times, and avoid direct contact with the reagents supplied in
this kit. PMS and 5鈥?-Fluorouracil can be harmful if ingested or inhaled. Extra care should be taken when
handling these components.
Section 4 : First Aid
Eye Contact: Check for and remove contact lenses. Flush eyes with running water for at least 15
minutes separating eyelids. Seek medical attention immediately
Skin Contact: Wash with soap and water for 15 minutes. Remove contaminated clothing and shoes.
If irritation or discomfort develops, seek medical attention.
Inhalation: Remove from exposure. If breathing is difficult, administer oxygen. If breathing stops,
administer artificial respiration. Seek medical attention if patient feels unwell or develops any
respiratory symptoms. Provide chemical label and MSDS if possible.
Ingestion: Remove dentures and clear mouth. If person is conscious, rinse mouth with water. Call
physician or poison control immediately. Provide chemical label and MSDS information if
possible.
Section 5 : Fire / Flammability Information
None of the components of this kit pose a significant risk in case of fire. Fire fighting media should be
selected to suit other materials involved in the fire. It is recommended that firefighters wear protective gear
and self contained breathing apparatus to limit exposure.
Section 6 : Spill or Leak Procedures.
Cleanup Procedures: Wearing appropriate protective gear as outlined under 鈥淧rotective equipment鈥?
wipe up spill, place in sealed container and hold for appropriate disposal. Wash spill site with soap
and water.
Waste Disposal Method: Dilute waste solutions and reagents with water and dispose of in accordance
with federal, state, and local regulations. Cells can be inactivated by autoclaving for 20 min at
121掳C or by incubating at pH 12 for 30 minutes before disposal.
Section 7 : Storage and Handling Precautions
Protective Equipment: For spill clean up, wear suitable protective clothing, chemical resistant rubber
gloves, rubber boots, and chemical safety goggles.
Storage and Handling: The components of this kit should be stored 鈥?70掳C. Plates containing cells
should not be thawed until immediately before use. Components should be kept in tightly closed
containers. This product should be handled only by qualified experienced professionals. Wash
thoroughly after handling the materials included in this kit.
Section 8 : Stability and Reactivity Data
Stability: This material is stable if stored as directed
Conditions to Avoid: Strong oxidizing agents, strong acids, strong bases
Incompatibles: Not available
Section 10 : Ecological Data
Ecotoxicological Information: Not Available
The above information is believed to be correct but does not purport to be complete and should be used only as a guide. The burden of
safe use of this material rests entirely with the user.
Emergency Contact:
BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting, PA 19462 USA
(610) 941-0430
Last Revised : 9/9/05
|