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Biomolecules for research                      MATERIAL SAFETY DATA SHEET







Section 1 : Substance Identification



              SUMOylation Kit

     Name:



     Catalog Number:    UW8955





     Company Identification:   BIOMOL International L.P.

                               5120 Butler Pike

                               Plymouth Meeting PA 19462-1202 USA

                               (610) 941-0430 (9am-5pm ET)









Section 2 : Components



     Vial #        Name:                                    Contents:   Quantity



     KW9330        20x SUMO Activating Enzyme Solution      1 µM        20 µl

                   (His6-Aos1/Uba2)



     KW9320        20x SUMO Conjugating Enzyme Solution     10 µM       20 µl

                   (UbcH9)



     KW9195        20x SUMO1 Enzyme Solution                5 µM        20 µl

                   (His6-mature-SUMO1)



     KW9205        20x SUMO2 Enzyme Solution                5 µM        20 µl

                   (His6-mature-SUMO2)



     KW9215        20x SUMO3 Enzyme Solution                5 µM        20 µl

                   (His6-mature-SUMO3)



     KW9890        10x SUMOylation Buffer                               40 µl



     KW9755        20x Control RANGAP1 SUMOylation          4 µM        20 µl

                   Target Protein Solution (RG1)

                   (GST-RANGAP1 fragment (418-587))

     KW9805        MgATP Solution                           0.1 M       20 µl

       PW9460          SUMO1 Antibody Solution                            Partially purified antibody       25 µl

                       Anti-SUMO1, (C-terminal)                           Supplied in PBS with

                       (rabbit, polyclonal)                               0.1% Sodium Azide



       PW9465          SUMO2/3 Antibody Solution                          Partially purified antibody       25 µl

                       Anti-SUMO2/3, (N-terminal)                         Supplied in PBS with

                       (rabbit, polyclonal)                               0.1% Sodium Azide







 Section 3 : Health Hazards



       Please be advised that the physical, chemical and toxicological properties of many of these components

       have not yet been fully investigated. It is therefore advised that all laboratory personnel handling this

       product follow standard laboratory safety procedures. It is recommended that personnel wear safety

       goggles, gloves, and protective clothing at all times, and avoid direct contact with the reagents supplied in

       this kit.









Section 4 : First Aid





       Eye Contact: Check for and remove contact lenses. Flush eyes with running water for at least 15

               minutes separating eyelids. Seek medical attention immediately

       Skin Contact: Wash with soap and water for 15 minutes. Remove contaminated clothing and shoes.

               If irritation or discomfort develops, seek medical attention.

       Inhalation: Remove from exposure. If breathing is difficult, administer oxygen. If breathing stops,

               administer artificial respiration. Seek medical attention if patient feels unwell or develops any

               respiratory symptoms. Provide chemical label and MSDS if possible.

       Ingestion: Remove dentures and clear mouth. If person is conscious, rinse mouth with water. Call

               physician or poison control immediately. Provide chemical label and MSDS information if

               possible.









 Section 5 : Fire / Flammability Information



       None of the components of this kit pose a significant risk in case of fire. Fire fighting media should be

       selected to suit other materials involved in the fire. It is recommended that firefighters wear protective gear

       and self contained breathing apparatus to limit exposure.









 Section 6 : Spill or Leak Procedures.





       Cleanup Procedures:       Wearing appropriate protective gear as outlined under “Protective equipment�

               wipe up spill area and rinse residue down the drain with plenty of water. Wash spill site with soap

               and water.

         Waste Disposal Method: Dilute waste solutions and reagents with water and dispose of in accordance

               with federal, state, and local regulations.







Section 7 : Storage and Handling Precautions



         Protective Equipment: For spill clean up, wear suitable protective clothing, chemical resistant rubber

                 gloves, rubber boots, and chemical safety goggles.

         Storage and Handling: The components of this kit should be stored �80°C. All components should be

                 kept in tightly closed containers. This product should be handled only by qualified experienced

                 professionals. Wash thoroughly after handling the materials included in this kit.









Section 8 : Stability and Reactivity Data



         Stability: This material is stable if stored as directed, avoid repeated freeze/thaw of components

         Conditions to Avoid: None known

         Incompatibles: Not available









Section 10 : Ecological Data



         Ecotoxicological Information: None Available







The above information is believed to be correct but does not purport to be complete and should be used only as a guide. The burden of

safe use of this material rests entirely with the user.





Emergency Contact:



BIOMOL International L.P.

5120 Butler Pike

Plymouth Meeting, PA 19462 USA



(610) 941-0430







Last Revised : 11/18/05