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Biomolecules for research MATERIAL SAFETY DATA SHEET
Section 1 : Substance Identification
Ubiquitinylation Kit
Name:
Catalog Number: UW9920
Company Identification: BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting PA 19462-1202 USA
(610) 941-0430 (9am-5pm ET)
Section 2 : Components
Vial # Name: Contents: Quantity
KW9410 20x Ubiquitin Activating Enzyme Solution 2 碌M 125 碌l
(His6-tagged, human, recombinant E1)
KW9020 10x Ubiquitin Conjugating Enzyme E2 0.5 mg/ml 20 碌l
Solution 鈥? UbcH1 (His6-tagged)
KW9025 10x Ubiquitin Conjugating Enzyme E2 0.5 mg/ml 20 碌l
Solution 鈥? UbcH2 (His6-tagged)
KW9030 10x Ubiquitin Conjugating Enzyme E2 0.5 mg/ml 20 碌l
Solution 鈥? UbcH3 (His6-tagged)
KW9050 10x Ubiquitin Conjugating Enzyme E2 0.5 mg/ml 20 碌l
Solution 鈥? UbcH5a (His6-tagged)
KW9060 10x Ubiquitin Conjugating Enzyme E2 0.5 mg/ml 20 碌l
Solution 鈥? UbcH5b (His6-tagged)
KW9070 10x Ubiquitin Conjugating Enzyme E2 0.5 mg/ml 20 碌l
Solution 鈥? UbcH5c (His6-tagged)
KW8710 10x Ubiquitin Conjugating Enzyme E2 0.5 mg/ml 20 碌l
Solution 鈥? UbcH6 (His6-tagged)
KW9080 10x Ubiquitin Conjugating Enzyme E2 0.5 mg/ml 20 碌l
Solution 鈥? UbcH7 (His6-tagged)
KW9135 10x Ubiquitin Conjugating Enzyme E2 0.5 mg/ml 20 碌l
Solution 鈥? UbcH8 (His6-tagged)
KW8715 10x Ubiquitin Conjugating Enzyme E2 0.5 mg/ml 20 碌l
Solution 鈥? UbcH10 (His6-tagged)
KW9565 10x Ubiquitin Conjugating Enzyme E2 0.5 mg/ml 20 碌l
Solution 鈥? Ubc13/Mms2 (His6-tagged)
KW8705 20x Biotinylated Ubiquitin Solution 50 碌M 125 碌l
(Bt-Ub)
KW9805 20x Mg-ATP Solution 0.1M 125 碌l
KW9880 2x Non-reducing Gel Loading Buffer 2.5 ml
KW9885 10x Ubiquitinylation Buffer 200 mM Tris-Cl, pH 75 250 碌l
Section 3 : Health Hazards
Please be advised that the physical, chemical and toxicological properties of many of these components
have not yet been fully investigated. It is therefore advised that all laboratory personnel handling this
product follow standard laboratory safety procedures. It is recommended that personnel wear safety
goggles, gloves, and protective clothing at all times, and avoid direct contact with the reagents supplied in
this kit.
Section 4 : First Aid
Eye Contact: Check for and remove contact lenses. Flush eyes with running water for at least 15
minutes separating eyelids. Seek medical attention immediately
Skin Contact: Wash with soap and water for 15 minutes. Remove contaminated clothing and shoes.
If irritation or discomfort develops, seek medical attention.
Inhalation: Remove from exposure. If breathing is difficult, administer oxygen. If breathing stops,
administer artificial respiration. Seek medical attention if patient feels unwell or develops any
respiratory symptoms. Provide chemical label and MSDS if possible.
Ingestion: Remove dentures and clear mouth. If person is conscious, rinse mouth with water. Call
physician or poison control immediately. Provide chemical label and MSDS information if
possible.
Section 5 : Fire / Flammability Information
None of the components of this kit pose a significant risk in case of fire. Fire fighting media should be
selected to suit other materials involved in the fire. It is recommended that firefighters wear protective gear
and self contained breathing apparatus to limit exposure.
Section 6 : Spill or Leak Procedures.
Cleanup Procedures: Wearing appropriate protective gear as outlined under 鈥淧rotective equipment鈥?
wipe up spill area and rinse residue down the drain with plenty of water. Wash spill site with soap
and water.
Waste Disposal Method: Dilute waste solutions and reagents with water and dispose of in accordance
with federal, state, and local regulations.
Section 7 : Storage and Handling Precautions
Protective Equipment: For spill clean up, wear suitable protective clothing, chemical resistant rubber
gloves, rubber boots, and chemical safety goggles.
Storage and Handling: The components of this kit should be stored 鈥?80掳C. All components should be
kept in tightly closed containers. This product should be handled only by qualified experienced
professionals. Wash thoroughly after handling the materials included in this kit.
Section 8 : Stability and Reactivity Data
Stability: This material is stable if stored as directed. Avoid repeated freeze/thaw of components
Conditions to Avoid: None known
Incompatibles: Not available
Section 10 : Ecological Data
Ecotoxicological Information: None Available
The above information is believed to be correct but does not purport to be complete and should be used only as a guide. The burden of
safe use of this material rests entirely with the user.
Emergency Contact:
BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting, PA 19462 USA
(610) 941-0430
Last Revised : 11/17/05
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