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MSDS 
:
 Denhardt's Reagent 50X Solution
CAS 
:
 7732-18-5
SYNONYMS 
:
 Blocking agent.
**** SECTION 2 - COMPOSITION, INFORMATION ON INGREDIENTS ****

+----------------+--------------------------------------+----------+-----------+
| CAS# | Chemical Name | % | EINECS# |
|----------------|--------------------------------------|----------|-----------|
|----------------|--------------------------------------|----------|-----------|
| 7732-18-5 |Water | 97 | 231-791-2 |
|----------------|--------------------------------------|----------|-----------|
| 9003-39-8 |Poly(vinylpyrrolidone) | 1 | unlisted |
|----------------|--------------------------------------|----------|-----------|
| 9048-46-8 |Bovine albumin | 1 | 232-936-2 |
|----------------|--------------------------------------|----------|-----------|
| 26873-85-8 |Sucrose-epichlorohydrin polymer (Ficol| 1 | unlisted |
+----------------+--------------------------------------+----------+-----------+
Text for R-phrases: see Section 16
Hazard Symbols: None Listed.
Risk Phrases: None Listed.

**** SECTION 3 - HAZARDS IDENTIFICATION ****

EMERGENCY OVERVIEW
Not available.

Potential Health Effects
Eye:
May cause eye irritation.
Skin:
May cause skin irritation.
Ingestion:
May cause irritation of the digestive tract. Low hazard for usual
industrial handling.
Inhalation:
May cause respiratory tract irritation. Low hazard for usual
industrial handling.
Chronic:
No information found.

**** SECTION 4 - FIRST AID MEASURES ****

Eyes:
Flush eyes with plenty of water for at least 15 minutes,
occasionally lifting the upper and lower eyelids. If irritation
develops, get medical aid.
Skin:
Flush skin with plenty of water for at least 15 minutes while
removing contaminated clothing and shoes. Get medical aid if
irritation develops or persists. Wash clothing before reuse.
Ingestion:
If victim is conscious and alert, give 2-4 cupfuls of milk or water.
Wash mouth out with water. Get medical aid if irritation or symptoms
occur.
Inhalation:
Remove from exposure and move to fresh air immediately. If not
breathing, give artificial respiration. If breathing is difficult,
give oxygen. Get medical aid if cough or other symptoms appear.
Notes to Physician:

**** SECTION 5 - FIRE FIGHTING MEASURES ****

General Information:
As in any fire, wear a self-contained breathing apparatus in
pressure-demand, MSHA/NIOSH (approved or equivalent), and full
protective gear. During a fire, irritating and highly toxic gases
may be generated by thermal decomposition or combustion. Use
extinguishing media appropriate to the surrounding fire. Substance is
noncombustible. Vapors may be heavier than air. They can spread
along the ground and collect in low or confined areas.
Extinguishing Media:
Substance is noncombustible; use agent most appropriate to
extinguish surrounding fire.

**** SECTION 6 - ACCIDENTAL RELEASE MEASURES ****

General Information: Use proper personal protective equipment as indicated
in Section 8.
Spills/Leaks:
Absorb spill with inert material (e.g. vermiculite, sand or earth),
then place in suitable container. Avoid runoff into storm sewers and
ditches which lead to waterways. Clean up spills immediately,
observing precautions in the Protective Equipment section. Provide
ventilation.

**** SECTION 7 - HANDLING and STORAGE ****

Handling:
Wash hands before eating. Avoid contact with eyes, skin, and
clothing. Avoid ingestion and inhalation. Use with adequate
ventilation.
Storage:
Store in a cool, dry, well-ventilated area away from incompatible
substances. No special precautions indicated. Store in freezer
(-20C - 0C).

**** SECTION 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION ****

Engineering Controls:
Facilities storing or utilizing this material should be equipped
with an eyewash facility and a safety shower. Use adequate
ventilation to keep airborne concentrations low.
Exposure Limits
CAS# 7732-18-5:

CAS# 9003-39-8:

CAS# 9048-46-8:

CAS# 26873-85-8:


Personal Protective Equipment

Eyes:
Wear appropriate protective eyeglasses or chemical
safety goggles as described by OSHA's eye and face
protection regulations in 29 CFR 1910.133 or European
Standard EN166.
Skin:
Wear appropriate protective gloves to prevent skin
exposure.
Clothing:
Wear appropriate protective clothing to minimize
contact with skin.
Respirators:
Follow the OSHA respirator regulations found in 29
CFR 1910.134 or European Standard EN 149. Use a
NIOSH/MSHA or European Standard EN 149 approved
respirator if exposure limits are exceeded or if
irritation or other symptoms are experienced.

**** SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES ****

Physical State: Liquid
Color: colorless
Odor: none reported
pH: Not available.
Vapor Pressure: Not available.
Viscosity: Not available.
Boiling Point: > 100 deg C
Freezing/Melting Point: < 0 deg C
Autoignition Temperature: Not applicable.
Flash Point: Not applicable.
Explosion Limits, lower: Not available.
Explosion Limits, upper: Not available.
Decomposition Temperature:
Solubility in water:
Specific Gravity/Density:
Molecular Formula: solution
Molecular Weight: 0

**** SECTION 10 - STABILITY AND REACTIVITY ****

Chemical Stability:
Stable at room temperature in closed containers under normal storage
and handling conditions.
Conditions to Avoid:
Excess heat.
Incompatibilities with Other Materials:
Water Reactive Substances., No significant incompatibilities
identified with common materials and contaminants..
Hazardous Decomposition Products:
Carbon monoxide, irritating and toxic fumes and gases, carbon
dioxide.
Hazardous Polymerization: Will not occur.

**** SECTION 11 - TOXICOLOGICAL INFORMATION ****

RTECS#:
CAS# 7732-18-5: ZC0110000
CAS# 9003-39-8: TR8160000 TR8170000 TR8180000 TR8250000
TR8300000 TR8350000 TR8360000 TR8370000
CAS# 9048-46-8: MT6446000
CAS# 26873-85-8 unlisted.
LD50/LC50:
CAS# 7732-18-5: Oral, rat: LD50 = >90 mL/kg.
CAS# 9003-39-8: Oral, mouse: LD50 = >40 gm/kg; Oral, rabbit: LD50 =
1040 mg/kg; Oral, rat: LD50 = 100 gm/kg.
CAS# 9048-46-8.
CAS# 26873-85-8.
Carcinogenicity:
Water -
Not listed by ACGIH, IARC, or NTP.
Poly(vinylpyrrolidone) -
Not listed by ACGIH, IARC, or NTP.
Bovine albumin -
Not listed by ACGIH, IARC, or NTP.
Sucrose-epichlorohydrin polymer (Ficoll 400) -
Not listed by ACGIH, IARC, or NTP.
Other:
See actual entry in RTECS for complete information.

**** SECTION 12 - ECOLOGICAL INFORMATION ****



**** SECTION 13 - DISPOSAL CONSIDERATIONS ****

Products which are considered hazardous for supply are classified as Special
Waste and the disposal of such chemicals is covered by regulations which may
vary according to location. Contact a specialist disposal company or the local
waste regulator for advice. Empty containers must be decontaminated before
returning for recycling.

**** SECTION 14 - TRANSPORT INFORMATION ****

IATA
Shipping Name: Not regulated.
Hazard Class:
UN Number:
Packing Group:
IMO
Shipping Name: Not regulated.
Hazard Class:
UN Number:
Packing Group:
RID/ADR
Shipping Name: Not regulated.
Hazard Class:
UN Number:
Packing group:

**** SECTION 15 - REGULATORY INFORMATION ****

European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols: Not available.
Risk Phrases:
Safety Phrases:
S 24/25 Avoid contact with skin and eyes.
WGK (Water Danger/Protection)
CAS# 7732-18-5: No information available.
CAS# 9003-39-8: 1
CAS# 9048-46-8: 0
CAS# 26873-85-8: No information available.
Canada
CAS# 7732-18-5 is listed on Canada's DSL List.
CAS# 9003-39-8 is listed on Canada's DSL List.
CAS# 9048-46-8 is listed on Canada's DSL List.
CAS# 26873-85-8 is listed on Canada's NDSL List.
CAS# 7732-18-5 is not listed on Canada's Ingredient Disclosure List.
CAS# 9003-39-8 is not listed on Canada's Ingredient Disclosure List.
CAS# 9048-46-8 is not listed on Canada's Ingredient Disclosure List.
CAS# 26873-85-8 is not listed on Canada's Ingredient Disclosure List.
US FEDERAL
TSCA
CAS# 7732-18-5 is listed on the TSCA inventory.
CAS# 9003-39-8 is listed on the TSCA inventory.
CAS# 9048-46-8 is listed on the TSCA inventory.
CAS# 26873-85-8 is listed on the TSCA inventory.

**** SECTION 16 - ADDITIONAL INFORMATION ****

Text for R-phrases from Section 2
MSDS Creation Date: 7/14/1999 Revision #4 Date: 5/20/2003

The information above is believed to be accurate and represents the best
information currently available to us. However, we make no warranty of
merchantability or any other warranty, express or implied, with respect to
such information, and we assume no liability resulting from its use. Users
should make their own investigations to determine the suitability of the
information for their particular purposes. In no way shall the company be
liable for any claims, losses, or damages of any third party or for lost
profits or any special, indirect, incidental, consequential or exemplary
damages, howsoever arising, even if the company has been advised of
the possibility of such damages.
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NAMECAS
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Decon 90 0-01-1
Zinc sulfate 0.05M solution 7732-18-5
Benzoylhydrazine, 98% 613-94-5
Iron (III) Chloride Solution 7705-08-0
Diisopropyl phosphite, 98% 1809-20-7
Benzyltriethylammonium Cyanate 0-00-1
Acrylonitrile 107-13-1
Ethanol, Absolute 64-17-5
L(+)Arabinose 87-72-9
Rennet 9001-98-3
Kjeldahl digestion reagent 7446-08-4
Kjeldahl phenol reagent (alkaline) 108-95-2
1,4-Benzodioxan-6-yl methyl ketone, 98% 2879-20-1
Indicator concentrate solution 61-73-4
Sodium Hydroxide/Sodium Thiosulfate Solution 1310-73-2
Oxygen mixture 1313-13-9
AA Flame Calibration Standard 7447-40-7
Chemicon A 139-33-3
Hydrochloric Acid, 28.0% 7647-01-0
Spectroflux R 100 12007-60-2
Chemicon B 7664-93-9
bis(Triphenylphosphine)dicarbonylnickel 13007-90-4
Solmix 64-17-5
Sodium acetate buffer solution 64-19-7
Polyurethane foam (polymer B) 0-00-0
Lanthanum Chloride Solution 7647-01-0
Martius yellow, biological stain and chemical indicator 605-69-6
Trans-Stilbene Oxide, 99% 1439-07-2
Sodium Acetate Buffer Solution with 5.0% Triton X-100 64-19-7
Trimethylamine 25% in methanol 65-55-0
Turpentine Substitute (White Spirit) 64742-88-7
CHAPSO 82473-24-3
Isonipecotamide, 98% 39546-32-2
CHAPS 75621-03-3
Xylene Cyanole FF 2650-17-1
Bovine Serum Albumin 9048-46-8
Lysozyme, Egg White 12650-88-3
BES 10191-18-1
TAPS (Free Acid) 29915-38-6
Ficoll 400 26873-85-8
PBS Phosphate Buffered Saline 7447-40-7
Denhardt's Reagent 50X Solution 7732-18-5
Cesium Sulfate, 98% (Titr.) 10294-54-9
Protein Gel Loading Dye 0-00-0
Denhardt's Reagent 50X Powder 150 mg 9003-39-8
Agarose Gel Loading Dye 0-00-0
Glycerol Gel Loading Dye 5X 0-00-0
Sequencing Gel Loading Dye 0-00-0

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