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MSDS  : 1,3-Naphthalenedisulfonic acid, 6,6'-((3,3'-dimethyl-4,4'-biphenylylene)bis(azo))bis (4-amino-5-hydroxy-, tetrasodium salt
CAS  : 314-13-6
SYNONYMS  : * Azovan Blue * 4,4'-Bis(1-amino-8-hydroxy-2,4-disulfo-7-naphthylazo)-3,3'-bitolyl, tetrasodium salt * 4,4'-Bis(7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo)-3,3'-bitolyl, tetrasodium salt * 4,4'-Bis(1-amino-8-hydroxy-2,4-disulpho-7-naphthylazo)-3,3'-bitolyl, tetrasodium salt * 4,4'-Bis(7-(1-amino-8-hydroxy-2,4-disulpho)naphthylazo)-3,3'-bitolyl, tetrasodium salt * Blekit evansa * C.I. 23860 * C.I. Direct Blue 53 * C.I. Direct Blue 53, tetrasodium salt * Diamine Sky Blue FF * Diazobleu * Diazol Pure Blue BF * Direct Blue 53 * Dye Evans Blue * Evablin * Evans Blue * Evans Blue Dye * Evans blue, sodium salt * Geigy-blau 536 * Geigy Blue 536, med * Modr Evansova * Modr Prima 53 * T 1824
*** CHEMICAL IDENTIFICATION ***

RTECS NUMBER : QJ6440000
CHEMICAL NAME : 1,3-Naphthalenedisulfonic acid,
6,6'-((3,3'-dimethyl-4,4'-biphenylylene)bis(azo))bis
(4-amino-5-hydroxy-, tetrasodium salt
CAS REGISTRY NUMBER : 314-13-6
LAST UPDATED : 199710
DATA ITEMS CITED : 26
MOLECULAR FORMULA : C34-H24-N6-O14-S4.4Na
MOLECULAR WEIGHT : 960.84
WISWESSER LINE NOTATION : L66J BZ CSWO ESWO JQ INUNR B1 DR C1 DNUN- IL66J BZ
CSWO ESWO JQ &-NA- 4
COMPOUND DESCRIPTOR : Tumorigen
Drug
Mutagen
Reproductive Effector
SYNONYMS/TRADE NAMES :
* Azovan Blue
* 4,4'-Bis(1-amino-8-hydroxy-2,4-disulfo-7-naphthylazo)-3,3'-bitolyl,
tetrasodium salt
* 4,4'-Bis(7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo)-3,3'-bitolyl,
tetrasodium salt
* 4,4'-Bis(1-amino-8-hydroxy-2,4-disulpho-7-naphthylazo)-3,3'-bitolyl,
tetrasodium salt
* 4,4'-Bis(7-(1-amino-8-hydroxy-2,4-disulpho)naphthylazo)-3,3'-bitolyl,
tetrasodium salt
* Blekit evansa
* C.I. 23860
* C.I. Direct Blue 53
* C.I. Direct Blue 53, tetrasodium salt
* Diamine Sky Blue FF
* Diazobleu
* Diazol Pure Blue BF
* Direct Blue 53
* Dye Evans Blue
* Evablin
* Evans Blue
* Evans Blue Dye
* Evans blue, sodium salt
* Geigy-blau 536
* Geigy Blue 536, med
* Modr Evansova
* Modr Prima 53
* T 1824

*** HEALTH HAZARD DATA ***

** ACUTE TOXICITY DATA **

TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 5 mL/kg
TOXIC EFFECTS :
Cardiac - other changes
Lungs, Thorax, or Respiration - acute pulmonary edema
REFERENCE :
ARSUAX Archives of Surgery (Chicago). (AMA, 535 N. Dearborn St., Chicago,
IL 60610) V.1-61, 1920-50; V.81- 1960- Volume(issue)/page/year:
48,17,1944

TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 340 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
BBRCA9 Biochemical and Biophysical Research Communications. (Academic
Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959-
Volume(issue)/page/year: 136,64,1986

TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 3 mL/kg
TOXIC EFFECTS :
Gastrointestinal - hypermotility, diarrhea
Gastrointestinal - nausea or vomiting
Lungs, Thorax, or Respiration - acute pulmonary edema
REFERENCE :
ARSUAX Archives of Surgery (Chicago). (AMA, 535 N. Dearborn St., Chicago,
IL 60610) V.1-61, 1920-50; V.81- 1960- Volume(issue)/page/year:
48,17,1944

TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 1 mL/kg
TOXIC EFFECTS :
Gastrointestinal - hypermotility, diarrhea
Liver - other changes
Kidney, Ureter, Bladder - changes in tubules (including acute renal failure,
acute tubular necrosis)
REFERENCE :
ARSUAX Archives of Surgery (Chicago). (AMA, 535 N. Dearborn St., Chicago,
IL 60610) V.1-61, 1920-50; V.81- 1960- Volume(issue)/page/year:
48,17,1944

TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 1 mL/kg
TOXIC EFFECTS :
Cardiac - other changes
Lungs, Thorax, or Respiration - acute pulmonary edema
REFERENCE :
ARSUAX Archives of Surgery (Chicago). (AMA, 535 N. Dearborn St., Chicago,
IL 60610) V.1-61, 1920-50; V.81- 1960- Volume(issue)/page/year:
48,17,1944

** OTHER MULTIPLE DOSE TOXICITY DATA **

TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 620 mg/kg/10D-I
TOXIC EFFECTS :
Related to Chronic Data - death
REFERENCE :
BBRCA9 Biochemical and Biophysical Research Communications. (Academic
Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959-
Volume(issue)/page/year: 136,64,1986

** TUMORIGENIC DATA **

TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 850 mg/kg/34W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
Blood - leukemia
REFERENCE :
APHGBP Acta Pathologica (Belgrade). (Belgrade Univerzitet, Institute de
Pathologie, Belgrade, Yugoslavia) V.2/3- 1938- Volume(issue)/page/year:
33,1,1953

** REPRODUCTIVE DATA **

TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 70 mg/kg
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
Reproductive - Specific Developmental Abnormalities - Central Nervous System
Reproductive - Specific Developmental Abnormalities - eye/ear
REFERENCE :
PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
(Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1903/04-
Volume(issue)/page/year: 127,215,1968

TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 70 mg/kg
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
REFERENCE :
PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
(Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1903/04-
Volume(issue)/page/year: 127,215,1968

TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 140 mg/kg
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - other effects to embryo
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 2,85,1969

TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE : 150 mg/kg
SEX/DURATION : female 7-9 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - Central Nervous System
REFERENCE :
ANREAK Anatomical Record. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY
10003) V.1- 1906/08- Volume(issue)/page/year: 118,369,1954

** MUTATION DATA **

TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 33 ug/plate
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 3,21,1982

TYPE OF TEST : Phage inhibition capacity
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 100 mmol/L
REFERENCE :
MDMIAZ Medycyna Doswiadczalna i Mikrobiologia. For English translation, see
EXMMAV. (Ars Polona, POB 1001, 00-068 Warsaw 1, Poland) V.1- 1949-
Volume(issue)/page/year: 31,11,1979

TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 100 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 136,255,1984

TYPE OF TEST : Unscheduled DNA synthesis
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 200 mg/kg
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 7,101,1985

TYPE OF TEST : DNA adduct
ROUTE OF EXPOSURE : Administration onto the skin
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 192 umol/kg
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 5,231,1984

TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 1001 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 189,223,1987

TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - hamster Liver
DOSE/DURATION : 10 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 136,255,1984

*** REVIEWS ***

IARC Cancer Review:Animal Limited Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 8,151,1975

IARC Cancer Review:Human No Adequate Data
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 8,151,1975

IARC Cancer Review:Group 3
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,56,1987

TOXICOLOGY REVIEW
ADTEAS Advances in Teratology. (New York, NY) V.1-5, 1966-72.
Discontinued. Volume(issue)/page/year: 1,131,1966

*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***

NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - T0844
No. of Facilities: 162 (estimated)
No. of Industries: 3
No. of Occupations: 6
No. of Employees: 5350 (estimated)
No. of Female Employees: 2829 (estimated)

*** STATUS IN U.S. ***

EPA GENETOX PROGRAM 1988, Inconclusive: Histidine reversion-Ames test

EPA TSCA Section 8(b) CHEMICAL INVENTORY

EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998


*** END OF RECORD ***
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ALL Chemical Property And Toxicity Analysis PAGES IN THIS GROUP
NAMECAS
2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy- 90-20-0
2,7-Naphthalenedisulfonic acid, 4-amino-3-hydroxy-,disodium salt 42579-07-7
2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-,disodium salt 3963-80-2
1,3-Naphthalenedisulfonic acid,4-amino-5-hydroxy-6-((4'-((2-hydroxy-1-naphthalenyl)azo)-3,3'-dimethoxy(1, 1'-biphenyl)-4-yl)azo)-,disodium salt 2586-57-4
2,7-Naphthalenedisulfonic acid,4-amino-5-hydroxy-6-((4'-((4-hydroxyphenyl)azo)(1,1'-biphenyl)-4-yl)azo)-3-((4-nitrophenyl)azo)-,disodium salt 4335-09-5
2,6-Naphthalenedisulfonic acid,4-amino-5-hydroxy-3-((4'-((4-hydroxyphenyl)azo)(1,1'-biphenyl)-4-yl)azo)-6-(phenylazo)-, disodium salt 3626-28-6
2-Naphthaleneacetic acid, 6-methoxy-alpha-methyl-,2-(4-nicotinoyl-1-piperazinyl) ethyl ester,dihydrochloride, (S)- 97699-66-6
2,7-Naphthalenedisulfonic acid,4-amino-5-hydroxy-3-((4-nitrophenyl)azo)-6-(phenylazo)-, disodium salt 1064-48-8
2,7-Naphthalenedisulfonic acid,4-amino-5-hydroxy-6-phenylazo-, disodium salt 3567-66-6
3,6-Naphthalenedisulfonic acid, 8-amino-7-hydroxy-,sodium salt 2007-20-7
2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-,p-toluenesulfonate (ester) 6837-93-0
2,7-Naphthalenedisulfonic acid,3-amino-4-((4'-((4-(((4-methylphenyl)sulfonyl)oxy)phenyl) azo)(1,1'-biphenyl)-4-yl)azo)-, disodium salt 2429-80-3
2,7-Naphthalenedisulfonic acid,4-((4-anilino-5-sulfo-1-naphthyl)azo)-5-hydroxy-,trisodium salt 3861-73-2
2,7-Naphthalenedisulfonic acid,3,3'-((4,4'-biphenylylene)bis(azo))bis(5-amino-4-hydroxy-, tetrasodium salt 2602-46-2
1,5-Naphthalenedisulfonic acid,3-((p-(bis(2-chloroethyl)amino)phenyl)azo)- 101931-20-8
2,7-Naphthalenedisulfonic acid,3,6-(bis(4-((2-hydroxyethyl)sulfonyl)phenyl)bis(azo))- 5-amino-4-hydroxy-, di(hydrogen sulfate) ester,tetrasodium salt 17095-24-8
1,3-Naphthalenedisulfonic acid,7-(3-(p-butoxyphenyl)-2-thioureido)-, dipotassiumsalt 37556-92-6
2-Naphthaleneacetic acid, 6-methoxy-alpha-methyl-,2-(4-(1-oxo-3-phenyl-2-propenyl)-1- piperazinyl)ethylester, monohydrochloride, (S)- 97699-78-0
1,5-Naphthalenedisulfonic acid,3-(3-(p-butoxyphenyl)-2-thioureido)-, disodium salt 37556-91-5
2,7-Naphthalenedisulfonic acid,4-(2-carboxyphenylazo)-3-hydroxy-, trisodium salt 1836-22-2
2,7-Naphthalenedisulfonic acid,4-(4-chloro-6-ethylphenylamino-2-s-triazinylamino)-5-hydroxy-6-(4-methyl-2-sulfophenylazo)-, trisodiumsalt 73816-74-7
2,7-Naphthalenedisulfonic acid,5-((4-chloro-6-(phenylamino)-1,3,5-triazin-2-yl)amino)-4- hydroxy-3-((2-sulfophenyl)azo)-, trisodium salt 23211-47-4
1,5-Naphthalenedisulfonic acid, 3,?-diamino- 39699-08-6
3,6-Naphthalenedisulfonic acid,1,7-diamino-8-hydroxy- 3545-88-8
2,6-Naphthalenedisulfonic acid, 3,7-di-tert-butyl-,compd. with 6-(dimethylamino)-4,4-diphenyl-3-hexanone 103442-82-6
2,7-Naphthalenedisulfonic acid,3,3'-((3,3'-dichloro-4,4'-biphenylylene)bis(azo))bis(5-amino-4-hydroxy-, tetrasodium salt 47897-65-4
2,7-Naphthalenedisulfonic acid,4-(4,6-dichloro-s-triazin-2-ylamino)-5-hydroxy-6-(2-hydroxy- 5-nitrophenylazo)- 73826-58-1
2,7-Naphthalenedisulfonic acid,5-(3,5-dichloro-s-triazinylamino)-4-hydroxy-3-phenylazo- 6522-86-7
2-Naphthaleneacetic acid, 6-methoxy-alpha-methyl-,2-(4-phenyl-1-piperazinyl)ethyl ester, (S)- 97699-76-8
2,7-Naphthalenedisulfonic acid,5-((4,6-dichloro-s-triazin-2-yl)amino)-4-hydroxy-3-(phenylazo)-, disodium salt 17804-49-8
1,5-Naphthalenedisulfonic acid,2-(6-(4,6-dichloro-s-triazinyl)methylamino-1-hydroxy-3- sulfonaphthylazo)- 73816-75-8
2,7-Naphthalenedisulfonic acid,4,5-dihydroxy-3-(phenylazo)-, disodium salt 4197-07-3
2,7-Naphthalenedisulfonic acid,3,3'-((3,3'-dimethoxy-4,4'-biphenylylene)bis(azo))bis( 5-amino-4-hydroxy-, tetrasodium salt 2429-74-5
6,8-Naphthalenedisulfonic acid,3,3'-((3,3'-dimethoxy-4,4'-biphenylene)bis(azo))bis(5-amino-4-hydroxy-, tetrasodium salt 2610-05-1
1,3-Naphthalenedisulfonic acid,6,6'-((3,3'-dimethyl-4,4'-biphenylylene)bis(azo))bis(4-amino-5-hydroxy-, tetrasodium salt 314-13-6
2,7-Naphthalenedisulfonic acid,3,3'-((3,3'-dimethyl-4,4'-biphenylylene)bis(azo))bis(5- amino-4-hydroxy-, tetrasodium salt 72-57-1
1,3-Naphthalenedisulfonic acid,8-((3,3'-dimethyl-4'-((4-(((4-methylphenyl)sulfonyl)oxy) phenyl)azo)(1,1'-biphenyl)-4-yl)azo)-7-hydroxy-,disodium salt 6459-94-5
1,3-Naphthalenedisulfonic acid,8-((4'-((4-ethoxyphenyl)azo)-3,3'-dimethyl(1,1'-biphenyl)-4- yl)azo)-7-hydroxy-, disodium salt 6358-29-8
1,3-Naphthalenedisulfonic acid, 7-hydroxy-, disodiumsalt 842-19-3
2-Naphthaleneacetic acid, 6-methoxy-alpha-methyl-,(1,4-piperazinediyl)diethylene ester, (S-(R*,R*))- 97699-69-9
2,7-Naphthalenedisulfonic acid, 3-hydroxy-, disodiumsalt 135-51-3
2,7-Naphthalenedisulfonic acid,4-hydroxy-3-((4'-((2-hydroxy-1-naphthalenyl)azo)-2,2'- dimethyl(1,1'-biphenyl)-4-yl)azo)-, disodium salt 3701-40-4
2,6-Naphthalenedisulfonic acid,4-hydroxy-3-((2-methoxy-5-methylphenyl)azo)-,disodium salt 5864-85-7
1,3-Naphthalenedisulfonic acid,7-hydroxy-8-((4'-((4-(((4-methylphenyl)sulfonyl)oxy)phenyl) azo)(1,1'-biphenyl)-4-yl)azo)-, disodium salt 3567-65-5
2,7-Naphthalenedisulfonic acid,3-hydroxy-4-(1-naphthalenylazo)-, disodium salt 5858-33-3
2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-nitroso-,disodium salt 525-05-3
2-Naphthalenesulfonic acid,4-hydroxy-3-((3-nitro-5-sulfophenyl)azo)-6-((3-((4-nitrophenyl) azo)-4,6-dihydroxyphenyl)azo)-, disodiumsalt 525-05-3
1,3-Naphthalenedisulfonic acid,7-hydroxy-8-(phenylazo)-, disodium salt 1936-15-8
2,7-Naphthalenedisulfonic acid,3-hydroxy-4-((4-sulfo-1-naphthalenyl)azo)-, trisodiumsalt 2611-82-7
2,7-Naphthalenedisulfonic acid,3-hydroxy-4-((4-sulfo-1-naphthyl)azo)-, trisodiumsalt 915-67-3

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