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File Name: toyobo_co_jp---dia_inurin.asp
                                   AMZ








GFR gold standard









L/min L/min
GFR

GFR





100


(GFR)

(GFR)

GFR
Ccr)
Ccr

Ccr
GFR
C GFR
Ccr



GFR gold standard
Cin)


GFR

GFR Ccr


Cin





GFR






1- -5-
(1-m-PMS) 5- -D-
1-m-PMS 1-m-PMS
N-
-N-(2- -3- )-m- (EHSPT) 4-
AA)


+

D-
+ -m-PMS
5- -D- + 1-m-PMS

1-m-PMS + O2 1-m-PMS + H2O2


2H2O2 + 4-AA + EHSPT + 4H2O




A 20mL 6

A D-
No. KTIN-501)
1- -5- A 20 L 6


4- B 20mL 6

B
No. KTIN-511)
N- -N-(2- -3- B 20 L 6
)-m-

(5mg/dL) 10mL 2

1 (4mg/dL) 10mL 2
No. KTIN-001)
2 (16mg/dL) 10mL 2





1.
(1) A A1 R1
(2) B B1 R2
(3)
(2 10 ) 2

.
(1)

0.3 mL
(0.3 m /L 0.9 mL


(3,000 rpm 10 )




10 40

(2)


10.8 L

- 10.8 L

10.8 L

10.8 L

R1 180 L 180 L 180 L 180 L

37 10

2 180 L 180 L 180 L 180 L

37 5

546nm 10mm


A D S B




.
(1)

( A B)
(mg/dL) 5
( S B)

( D B)
mg/dL) 5
( S B)

5 (mg/dL)
4


(mg/dL)

(2) (Cin)
Cin
U V 1.73
Cin(mL/min/1.73m2)
P A

(mL/min/1.73m2)
Cin
U (mg/dL)
P (mg/dL)
V (mL/min)
1.73
(m2)
A

A( ) Dubois
(m2) (kg)0.425 (cm)0.725 0.007184


. 7180




ASSAY 1 1
15 50 0 15 50 0
SAMPLE VOL. 7.2 7.2
A 120 120
B 120 120
SUB/MAIN) 800 546 800 546





(~30mg/dL) (~30mg/dL)




(mg/dL)
(%)

(%) 100
5.0 7.4 96.0
2.6
10.0 13.0 104.0
20.0 23.2 103.0
5.0 13.0 96.0
8.2
10.0 18.6 104.0
20.0 29.1 104.5




10 g/ L 20 g/ L

10 10

1 9.6 10.2 10.0 1 20.0 19.8 19.5
2 9.6 10.2 9.9 2 20.0 19.8 19.3
3 9.6 10.2 10.0 3 20.0 20.2 19.5
4 9.6 10.2 10.1 4 19.2 19.8 19.5
5 9.6 10.2 9.8 5 19.6 20.2 19.3
6 9.6 9.8 9.9 6 19.6 19.8 19.4
7 9.6 10.2 9.9 7 19.2 19.8 19.3
8 9.2 10.2 9.9 8 19.2 20.2 19.4
9 9.6 10.2 9.8 9 19.6 20.2 19.4
10 9.6 10.2 10.0 10 19.6 20.2 19.1
11 9.6 10.2 9.9 11 19.6 19.8 19.4
12 9.6 10.2 9.9 12 19.2 20.2 19.2
13 9.2 10.2 9.9 13 19.2 19.8 19.5
14 10.0 10.2 9.8 14 19.6 20.2 19.3
15 9.2 9.8 10.0 15 19.2 20.2 19.3
16 10.0 10.2 10.0 16 19.6 20.2 19.2
17 9.6 10.2 9.9 17 18.8 19.8 19.4
18 9.6 10.2 9.9 18 19.6 19.8 19.3
19 9.6 9.8 9.9 19 19.6 19.8 19.3
20 9.2 10.2 10.0 20 19.2 20.2 19.5
9.6 10.1 9.9 19.5 20.0 19.4
R 0.8 0.4 0.3 R 1.2 0.4 0.4
S.D. 0.221 0 0.1465 0.0754 S.D. 0.3205 0.2052 0.1146
CV CV
2.31 1.45 0.76 1.65 1.03 0.59




g/ L

( 10 1 10 2 10 3

1 4.7 10.4 16.8 5.1 10.2 16.2
2 4.8 10.4 16.9 5.1 10.4 16.4
3 5.1 10.6 16.5 5.0 10.0 16.1
4 4.8 10.3 16.2 4.8 9.9 15.6
5 4.9 10.4 16.6 4.9 10.1 15.8
6 4.6 10.5 16.6 4.9 10.0 16.0
7 4.7 10.4 16.4 5.0 10.2 16.2

4.8 10.4 16.6 5.0 10.1 16.0

R 0.5 0.3 0.7 0.3 0.5 0.8

S.D. 0.1633 0.0951 0.2360 0.1113 0.1676 0.2693

CV(%) 3.40 0.91 1.42 2.24 1.66 1.68





C F




10





72 176 mL/min/1.73m2
81 137 mL/min/1.73m2
Schuster,V.L, The Kidney : Physiology and Pathophysiology.
Raven Press, New York, 945(1992)




)
28 (2), 143-149(2005)
)


47 (7),804-812(2005)
43 (1),1-19(2001)

,20-28,57-70(2003)
32 ,1415-1427(2005)
Schuster,V.L, The Kidney : Physiology and Pathophysiology. Raven Press,
New York, 945(1992)
Levey, A. S., Kidney Int., 38 167-184(1990)
Shemesh, O., et.al., Kidney Int., 28 830-838(1985)
Tessitore, No., et. al. Nephron, 24 41-45(1979)
Monteiro, M. C. A., et. al. Braz. J. Med. Biol. Res., 27 2557-2564(1964)
Roy, L., et. al., Clin. Nephrol., 50 342-346(1998)
King, A. J., et. al., J. Am. Soc. Nephrol., 3 1723-1737(1993)
Laville, M., et. al., Nephron, 51 233-236(1989)
Levey, A. S., Am. J. Kidney Dis., 22 207-214(1993)
Chow, S. Y., Endocrinology, 77. 818-824(1965)
Keiding, S., Acta.Physiol. Scand. 133(3),335-342(1988)




2 10 24




A




(




/L

/




530-8230 28
TEL:06-6348-3335 FAX:06-6348-3833



10 24

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