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MSDS Material Safety Data Sheet
CAS

7647-14-5
77-86-1
497-19-8

File Name: neogen_com---7127_msds.asp
                                                                                                       Product # (s)_7127




MATERIAL SAFETY DATA SHEET
Section 1. Company Identification and Product Information
Product Name or Identity: Columbia Broth
Acumedia Manufacturers, Inc. 517/372-9200
Manufacturer鈥檚 Name: Emergency Phone No.:
740 East Shiawassee 517/372-0108
Fax No.:
Lansing, Michigan 48912 foodsafety@neogen.com
e-mail:
Date Prepared or Revised: January 2008

Section 2. Composition / Information on Hazardous Ingredients
Hazardous Components CAS-No. % EG-Number Hazard
Specific Chemical Identity: Symbol
Sodium Chloride, NaCl 7647-14-5 14.3% 231-598-3 Xi (Irritant)
Tris (hydroxymethyl) aminomethane-HCl 77-86-1 10.5% 201-064-4 Xi (Irritant)
Sodium Carbonate 497-19-8 1.7% N/A Xi (Irritant)

Section 3. Health Hazard Identification
Inhalation? Yes Skin? Yes Ingestion? Yes
Route(s) of Entry:
IRRITANT. Irritating to eyes, respiratory system, and skin. May be harmful if ingested or contact
Health Hazards:
with eyes.
(Acute and Chronic)
IARC Monographs? No OSHA Regulated? No
Carcinogenicity:
Signs and Symptoms of Exposure: Irritant if inhaled, coughing possible and breathing difficulties may be observed. May
be harmful if swallowed or inhaled. Sodium Carbonate can result in eye burns, conjuctival edema and corneal destruction.
Prolonged contact with skin can cause redness, dermatitis, and blisters.
Medical Conditions Generally Aggravated by Exposure: May be harmful if inhaled, causing respiratory tract irritation,
coughing and breathing difficulties. Gastrointestinal disturbances can occur if ingested, causing abdominal pain, vomiting,
and diarrhea.

Section 4. First Aid Measures
Ingestion: If swallowed, wash out mouth with water, provided person is conscious. Never give anything
Emergency /
by mouth to an unconscious person. Seek medical attention.
First Aid
Procedures: Inhalation: If inhaled, supply fresh air or oxygen. Seek medical attention. If not breathing, apply artificial
respiration. If breathing is difficult, give oxygen.
Eye Contact: Rinse opened eye for at least 15 minutes under running water, lifting lower and upper
eyelids occasionally. Seek medical attention.
Skin Contact: Remove contaminated clothing. Immediately wash with plenty of soap and water for at
least 15 minutes. Seek medical attention. Wash clothing before reuse.

Section 5. Fire and Explosion Hazard Data
Flash Point (Method Used): N/A Flammable Limits: LEL 鈥? N/A
UEL 鈥? N/A
Extinguishing Media: Use alcohol foam, dry chemical, or carbon dioxide. Water may be effective for cooling, but may not
effect extinguishment.
Special Fire Fighting Procedures: Firefighters should wear protective equipment and self-contained breathing apparatus.
The product itself does not burn.
Unusual Fire and Explosion Hazards: During heating or in case of fire, poisonous gases are produced. Fine dust
dispersed in air in sufficient concentrations, and in the presence of an ignition source, is a potential dust explosion hazard.




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Product # (s)_7127



Section 6. Accidental Release Measures
Personal Precautions: Shut off all sources of ignition, ventilate spill area. Wear suitable protective clothing, gloves, and
eye protection. Wear self-containing breathing apparatus, rubber boots, and heavy rubber gloves. Place contaminated
material in a chemical waste container. Prevent direct contact with skin, eyes, or clothing.
Environmental Precautions: Prevent dispersion of material. Do not allow to enter drains or water courses. Water runoff
can cause environmental damage.
Clean-up Methods: Contact safety officer and ventilate area. Absorb spill with inert material, including dry-lime, sand, or
soda ash, then place into a chemical waste container using non-sparking tools. Wash spill site.

Section 7. Handling and Storage
Handling: Protect against physical damage. Ensure good ventilation / exhaustion. Avoid contact with eyes, skin, and
clothing. Avoid prolonged or repeated exposure. Do not use if skin is cut or scratched.
Storage: Keep container tightly closed. Keep away from incompatible material. Storage area should be cool, dry and well
ventilated. Containers of this material may be hazardous when empty since they retain product residues.
Other Precautions: Remove contaminated clothing immediately. Ensure good ventilation. Prevent dust formation.

Section 8. Exposure Controls / Personal Protection
OES: N/A ACGIH 鈥? TLV: N/A
Engineering Measures: Respiratory protection is not required. Where protection from nuisance levels of dusts are
desired, use type N95 (US) or type P1 (EN 143) dust masks. Use respirators and components tested and approved under
appropriate government standards such as NIOSH (US) or CEN (EU). Proper ventilation, safety shower, and eye bath
required.
Respiratory Protection (Specify Type): With sufficient ventilation, breathing apparatus is not necessary. In the event of
possible spill / exposure, use dust mask to EN 149 FFP2S.
Local Exhaust: 50 鈥? 100 CFM Special: Safety shower and eye wash.
Ventilation:
Protective Gloves: Compatible chemical- Eye Protection: Safety glasses or chemical goggles to EN 166, 167,
resistant gloves. and 168.
Other Protective Clothing or Equipment: Uniform, lab coat, or disposable lab wear.
Work / Hygienic Practices: Follow the usual precautionary measure for handling chemicals / powder. Keep away from
food and beverages. Immediately remove all soiled and contaminated clothing. Avoid contact with eyes, skin, and clothing.

Section 9. Physical and Chemical Properties
Specific Gravity: 2.16 g/cm3 (NaCl), 2.5 g/cm3
Boiling Point: 1461掳C (NaCl), 220掳C (Tris) (Sodium Carbonate)

Melting Point: 171.2 (Tris)
Vapor Pressure: 1 mm at 865掳C (NaCl)
804 掳C (NaCl)
851 潞C (Sodium Carbonate),
Vapor Density (AIR = 1): N/A Solubility in Water: Partly soluble (NaCl)
Appearance and Odor: Solid, colorless or white, odorless (NaCl), White powder (Sodium Carbonate)
White or colorless, crystals (Tris)

Section 10. Stability and Reactivity
Unstable
Stability:
Stable X Conditions to Avoid: Moisture. Stable under recommended storage conditions.
Incompatibility (Materials to Avoid): Incompatible with strong oxidizing agents and strong acids.

Hazardous Decomposition or Byproducts: Carbon oxides, Nitrogen oxides, Sodium oxides, and Hydrogen chloride gas
May Occur
Hazardous Polymerization:
Will Not Occur X Conditions to Avoid: Heat, moisture, dusting, and incompatible
materials.

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Product # (s)_7127



Section 11. Toxicological Information
LD50: ORL-RAT, 3,000 mg/kg (Sodium Chloride)
LD50: ORL-RAT, 5,900 mg/kg (Tris (hydroxymethyl) aminomethane-HCl)
LD50: ORL-RAT, 4090 mg/kg (Sodium Carbonate)

Section 12. Ecological Information
Ecotoxicity Tests: LC50 1,294.6 mg/L, 96 hours, Lepomis macrochirus (Bluegill) (Sodium Chloride)
LC50 : 300 mg/L, 96 hours, Lepomis macrochirus (Bluegill) (Sodium Carbonate)

Section 13. Disposal Considerations
Waste Disposal Method: Dispose in accordance with all applicable federal, state, and local environmental regulations.
Keep waste separate. Contact a licensed professional waste disposal service to dispose of this material if questions arise.
Do not allow product to reach ground water, water bodies, or sewage system.
Container Information: Do not remove labels from containers until they have been cleaned.

Section 14. Transport Information
Sodium Chloride, Tris (hydroxymethyl) aminomethane-HCl, Sodium Carbonate:*
UN # --
Class:--
Packing Group: --
Hazard Class: --
IATA: Non-Hazardous for air transport
*These chemicals are not regulated for transportation and considered non-hazardous for transport.

Section 15. Regulatory Information
EU Regulations, Hazard Symbol(s):
Sodium Chloride: Xi (Irritant)
Tris (hydroxymethyl) aminomethane-HCl: Xi (Irritant)
Sodium Carbonate: Xi (Irritant)

Risk Phrases:
Sodium Chloride: R 36 / 38, Irritating to eyes and skin.
Tris (hydroxymethyl) aminomethane-HCl: R 36 / 37 / 38, Irritating to eyes, respiratory system, and skin.
Sodium Carbonate: R 36, Irritating to eyes.

Safety Phrases:
Sodium Chloride: S 24 / 25 / 26, Avoid contact with skin and eyes. In case of contact with eyes, rinse immediately with
plenty of water and seek medical advice.
Tris (hydroxymethyl) aminomethane-HCl: S 26 / 36, In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice. Wear suitable protective clothing.
Sodium Carbonate: S 22 / 26, Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.

Section 16. Other Information
This document is believed to be correct, but does not purport to be all inclusive and shall be used only as a guide.
Acumedia Manufacturers, Inc. shall not be held liable for any damage resulting from handling or from contact with the above
product. These suggestions should not be confused with state, municipal or insurance requirements, and constitute NO
WARRANTY.


Page 3 of 3

Rev 04, January 2008

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