Material Safety Data Sheet
Revision date: 01/17/2005
Print Date: 02/09/2005
Version: 1
1. IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND THE COMPANY/UNDERTAKING
Product name: X-tremeGENE siRNA Dicer Kit
Product number: 04579020001
Business area: Roche Applied Sciences.
Product line: Not applicable
Supplier: Emergency telephone number:
Roche Diagnostics Corporation CHEMTREC:
9115 Hague Road 1-800-424-9300 (U.S. or Canada)
Indianapolis, IN 46250 1-703-527-3887 (International)
Site Phone Number: 1-800-428-5074
2. COMPOSITION/INFORMATION ON INGREDIENTS
Component 1: Bottle 1
Components CAS Number Weight % OSHA PEL: OSHA STEL: ACGIH TLV: ACGIH STEL:
Glycerol 56-81-5 50 - 60 15 mg/m鲁 NA 10 mg/m鲁 NA
5 mg/m鲁
Potassium chloride 7447-40-7 1-5 NA NA NA NA
Component 2: Bottle 2
Components CAS Number Weight % OSHA PEL: OSHA STEL: ACGIH TLV: ACGIH STEL:
Tris hydrochloride 1185-53-1 1-5 NA NA NA NA
Component 3: Bottle 3
Components CAS Number Weight % OSHA PEL: OSHA STEL: ACGIH TLV: ACGIH STEL:
Glycerol 56-81-5 50 - 60 15 mg/m鲁 NA 10 mg/m鲁 NA
5 mg/m鲁
Component 4: Bottle 4
Components CAS Number Weight % OSHA PEL: OSHA STEL: ACGIH TLV: ACGIH STEL:
Tris hydrochloride 1185-53-1 1-5 NA NA NA NA
HEPES 7365-45-9 1-5 NA NA NA NA
Component 5: Bottle 5
Components CAS Number Weight % OSHA PEL: OSHA STEL: ACGIH TLV: ACGIH STEL:
Proteinase K 39450-01-6 1-5 NA NA NA NA
Component 6: All other bottles
Components CAS Number Weight % OSHA PEL: OSHA STEL: ACGIH TLV: ACGIH STEL:
No hazardous components >1%, NONE N/A NA NA NA NA
>0.1% carcinogen
3. HAZARDS IDENTIFICATION
Emergency Overview
NFPA Ratings: Health= 1. Flammability= 0 Reactivity= 0 Special= I.
Special Definitions: A=Allergen CA=Carcinogen CO=Corrosive F=Flammable H=Harmful I=Irritant Ox=Oxidizer PB=Potential Biohazard
R=Reproductive S=Sensitizer T=Toxic T+=Highly Toxic W=Water Reactive
Principle routes of exposure: Ingestion, inhalation, skin and/or eye contact.
Inhalation: Avoid breathing vapors or mists. May cause irritation of respiratory tract.
Ingestion: Ingestion may cause gastrointestinal irritation, nausea, vomiting and
diarrhea
Skin contact: Substance may cause slight skin irritation.
Eye contact: Contact with eyes may cause irritation
Sensitization: The following chemical(s) may cause sensitization:
Medical conditions aggravated by exposure: None known
Additional information: None
Target organ effects: The following chemical(s) have target organ effects:
Components NIOSH - Pocket Guide - Target Organs:
Glycerol respiratory system, skin, eyes, kidneys
Components NIOSH - Pocket Guide - Target Organs:
Glycerol respiratory system, skin, eyes, kidneys
4. FIRST AID MEASURES
Inhalation: Consult a physician. Move to fresh air. If not breathing, give artificial respiration. If breathing is difficult,
give oxygen
Skin contact: Rinse with plenty of water. If skin irritation persists, call a physician. Remove and wash contaminated
clothing before re-use
Ingestion: Consult a physician. Do not induce vomiting without medical advice
Eye contact: In the case of contact with eyes, rinse immediately with plenty of water and seek medical advice
Notes to physician: None determined
5. FIRE-FIGHTING MEASURES
Suitable extinguishing media: Use dry chemical, co2, water spray or `alcohol` foam
Unusual hazards: None known
Special protective equipment for firefighters: Wear self contained breathing apparatus for fire fighting if necessary
6. ACCIDENTAL RELEASE MEASURES
Personal precautions: Use personal protective equipment. Evacuate personnel to safe areas
Environmental precautions: Prevent further leakage or spillage if safe to do so. Prevent product from entering drains.
Methods for cleaning up: Never return spills in original containers for re-use. Sweep up or vacuum (if powder) or soak up with inert
absorbent material (if liquid), then place into a suitable clean, dry, closed container, and label for disposal
7. HANDLING AND STORAGE
Handling: Protect from contamination. Wear personal protective equipment
Storage: No special precaustions required
8. EXPOSURE CONTROLS / PERSONAL PROTECTION
Engineering measures: Not applicable
Personal Protective Equipment
Respiratory protection: Respiratory protection is not required under normal use of this product. If respiratory protection is needed,
follow the OSHA regulation, 29CFR1910.134. Always use a NIOSH approved respirator when necessary
Hand protection: Wear appropriate protective gloves to prevent skin contact. Replace torn or punctured gloves promptly
Skin and body protection: Wear appropriate body protection to prevent skin contact
Eye protection: Wear appropriate eye protection to prevent eye contact
Product number: 04579020001 Page 2 of 5
Product name: X-tremeGENE siRNA Dicer Kit
Hygiene measures: Avoid contact with skin, eyes and clothing
Exposure limit(s)
9. PHYSICAL AND CHEMICAL PROPERTIES
Boiling point/range: No information available
Color: Colourless.
Odor: Bottles 1 & 2=modest odor. All other bottles=None.
pH: Bottle 8=4. All other bottles=7-9.
Physical state: Liquid.
10. STABILITY AND REACTIVITY
Stability: Stable under recommended storage conditions
Polymerization: Not applicable
Hazardous decomposition products: Carbon monoxide
Materials to avoid: Strong acids and strong bases
11. TOXICOLOGICAL INFORMATION
Components EPA-IRIS Oral Reference Dose EPA-IRIS Inhalation Reference NIOSH -
(RfD) Information: Concentration (RfC) Information: Selected LD50s and LC50s:
Glycerol = 12600 mg/kg Oral LD50 Rat
= 4090 mg/kg Oral LD50 Mouse
> 10 g/kg Dermal LD50 Rabbit
> 570 mg/m鲁 Inhalation LC50 Rat 1 h
Potassium chloride = 1500 mg/kg Oral LD50 Mouse
= 2600 mg/kg Oral LD50 Rat
Components EPA-IRIS Oral Reference Dose EPA-IRIS Inhalation Reference NIOSH -
(RfD) Information: Concentration (RfC) Information: Selected LD50s and LC50s:
Glycerol = 12600 mg/kg Oral LD50 Rat
= 4090 mg/kg Oral LD50 Mouse
> 10 g/kg Dermal LD50 Rabbit
> 570 mg/m鲁 Inhalation LC50 Rat 1 h
Inhalation: No data available
Skin: No data available
Oral: No data available
Mutagenic effects: No data is available on the product itself
Reproductive toxicity: No data is available on the product itself
Carcinogenic effects:
12. ECOLOGICAL INFORMATION
Bioaccumulation: Not determined
Aquatic toxicity: Not applicable
Ecotoxicity effects:
Components Ecotoxicity - Ecotoxicity - Ecotoxicity - Ecotoxicity -
Fish Species Data Microtox Data Water Flea Data Freshwater Algae Data
Glycerol = 50 mg/L LC50 rainbow
trout 96 h
> 5000 mg/L LC50 goldfish
96 h
Product number: 04579020001 Page 3 of 5
Product name: X-tremeGENE siRNA Dicer Kit
Components Ecotoxicity - Ecotoxicity - Ecotoxicity - Ecotoxicity -
Fish Species Data Microtox Data Water Flea Data Freshwater Algae Data
Glycerol = 50 mg/L LC50 rainbow
trout 96 h
> 5000 mg/L LC50 goldfish
96 h
13. DISPOSAL CONSIDERATIONS
Waste from residues / unused products: Waste disposal must be in accordance with appropriate Federal, State, and local regulations. This product, if
unaltered by use, may be disposed of by treatment at a permitted facility or as advised by your local hazardous waste regulatory authority. Residue from
fires extinguished with this material may be hazardous.
14. TRANSPORT INFORMATION
Is product hazardous to ship? No
DOT
Proper shipping name: None
UN Number: Not applicable Hazard class: Not applicable Subsidiary risk: Not applicable Packing group: Not applicable
ICAO/IATA
Proper shipping name: None
UN Number: Not applicable Hazard class: Not applicable Subsidiary risk: Not applicable Packing group: Not applicable
15. REGULATORY INFORMATION
U.S. Regulations:
U.S. TSCA/SARA/CERCLA Regulatory Information: The following chemicals are listed under the following TSCA/SARA/CERCLA lists
Components CERCLA/SARA 302 CERCLA/SARA 304 SARA 313 TSCA Inventory
RQ and TPQ RQ Emission reporting
(40 CFR 355, App.A) (40 CFR Table 302.4)
Glycerol Present
Potassium chloride XU
Components CERCLA/SARA 302 CERCLA/SARA 304 SARA 313 TSCA Inventory
RQ and TPQ RQ Emission reporting
(40 CFR 355, App.A) (40 CFR Table 302.4)
Tris hydrochloride Present
Components CERCLA/SARA 302 CERCLA/SARA 304 SARA 313 TSCA Inventory
RQ and TPQ RQ Emission reporting
(40 CFR 355, App.A) (40 CFR Table 302.4)
Glycerol Present
Components CERCLA/SARA 302 CERCLA/SARA 304 SARA 313 TSCA Inventory
RQ and TPQ RQ Emission reporting
(40 CFR 355, App.A) (40 CFR Table 302.4)
Tris hydrochloride Present
HEPES Present
U.S. Clean Air Act (CAA): The following chemicals are listed under the following CAA lists
Components CAA - CAA - CAA-Volatile Organic Compounds
1990 Hazardous Air Pollutants Class 1 Ozone Depletors (VOCs) in SOCMI
Glycerol Present
Product number: 04579020001 Page 4 of 5
Product name: X-tremeGENE siRNA Dicer Kit
Components CAA - CAA - CAA-Volatile Organic Compounds
1990 Hazardous Air Pollutants Class 1 Ozone Depletors (VOCs) in SOCMI
Glycerol Present
U.S. Clean Water Act (CWA): The following chemicals are listed under the following CWA lists
California Safe Drinking Water and Toxic Enforcement Act (Prop 65): The following chemicals are listed under the California Prop 65 list
Canadian Regulations:
Canada DSL Inventory List - This product complies with DSL
Canada NDSL Inventory List - This product does not comply with NDSL
Components Canada - WHMIS: Classifications of Substances:
Glycerol Uncontrolled product according to WHMIS classification criteria.
Potassium chloride Uncontrolled product according to WHMIS classification criteria.
Components Canada - WHMIS: Classifications of Substances:
Tris hydrochloride D2B
Components Canada - WHMIS: Classifications of Substances:
Glycerol Uncontrolled product according to WHMIS classification criteria.
Components Canada - WHMIS: Classifications of Substances:
Tris hydrochloride D2B
16. OTHER INFORMATION
Reason for revision: Not applicable
References: None
Additional advice: None
Prepared by: Roche Diagnostics Corporation, Safety and Environmental Department, MSDS Contact: 317-521-7425
The information, data and recommendations contained herein are based upon information believed by Roche Diagnostics Corporation ("RDC"), after reasonable investigation
and research, to be accurate; however, RDC does not warrant the accuracy of this information. All materials and mixtures may present unknown hazards and should be used
with caution. When necessary or appropriate, independent opinions regarding the risk of handling or exposure should be obtained from trained professionals. RDC disclaims
any warranty against patent infringement and the implied warranties of merchantability and fitness for a particular purpose. Customer's sole and exclusive remedy shall be
replacement of the product or return of the product and refund of the purchase price, at RDC's option. In no case shall RDC be liable for incidental or consequential
damages, including lost profits.
End of Safety Data Sheet
Product number: 04579020001 Page 5 of 5
Product name: X-tremeGENE siRNA Dicer Kit
|