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MSDS Material Safety Data Sheet
CAS

64-17-5
108-10-1
110-54-3
108-73-6

File Name: carolina_com---phloroglusol.asp
                    MATERIAL SAFETY DATA SHEET
Revised: 16/07/04
PHLOROGLUCINOL STAINING SOLUTION Replaces: 09/05/00
CAROLINA BIOLOGICAL Printed: 20/05/08


1. PRODUCT DESCRIPTION
Product Name: Phloroglucinol Staining Solution
Product Code(s): 88-1321, 88-1323, 88-1325, B6080
Size: 25 ml, 120 ml, 500 ml
Chemical Name: Does not apply, product is a mixture
CAS Number: See section 2
Formula: See Section 2
Synonyms: None
Distributor: Carolina Biological Supply Company
2700 York Road
Burlington, NC 27215
Chemical Information: 800-227-1150 (8am-5pm (ET) M-F)
Chemtrec (Transportation Spill Response 24 hours): 800-424-9300
2. COMPOSITION/INFORMATION ON INGREDIENTS
Principle Hazardous Components:
Ethyl alcohol (CAS#64-17-5) 87%
Methyl isobutyl ketone (Denaturant) (CAS# 108-10-1) 4%
Hexane (Denaturant) (CAS#110-54-3) 0.8%
Phloroglucinol (CAS#108-73-6) 8%
TLV and PEL units:
Ethyl alcohol: ACGIH-TLV 1000ppm (TWA)
OSHA-PEL 1000ppm (TWA)
Methyl isobutyl ketone: ACGIH-PEL 50 ppm (TWA), STEL 75 ppm;
OSHA-PEL 50 ppm (TWA), STEL 75 ppm
Hexane: ACGIH-TLV 50 ppm (TWA), OSHA-PEL 50 ppm (TWA)
3. HAZARD IDENTIFICATION
Emergency Overview: Concentrations below 1,000 ppm usually produce no
signs
of intoxication. Exposure to concentrations over 1,000 ppm may cause
headache, irritation of the eyes, nose, and throat, and if continued for
an
hour, drowsiness and lassitude, loss of appetite and inability to
concentrate.
Potential Health Effects:
Eyes: May cause irritation.
Skin: May cause irritation.
Ingestion: May cause gastrointestinal discomfort.
Inhalation: May cause irritation to respiratory tract.
4. FIRST AID MEASURES
Emergency and First Aid Procedures:
Eyes - Flush with water for at least 15 minutes, raising and lowering
eyelids occasionally. Get medical attention if irritation persists.
Skin - Thoroughly wash exposed area for at least 15 minutes. Remove
contaminated clothing. Launder contaminated clothing before reuse. Get
medical attention if irritation persists.
Ingestion - If swallowed, if conscious, give plenty of water.
Immediately call a physician or poison control center. Never give
anything by mouth to an unconscious person.
Inhalation - Remove to fresh air. Give oxygen if breathing is
difficult; give artificial respiration if breathing has stopped. Keep
person warm and quiet, and get medical attention.
Page 1 of 4
MATERIAL SAFETY DATA SHEET
Revised: 16/07/04
PHLOROGLUCINOL STAINING SOLUTION Replaces: 09/05/00
CAROLINA BIOLOGICAL Printed: 20/05/08


5. FIREFIGHTING PROCEDURES
Flash Point(Method Used):(cc)55.6F-Ethyl alcohol
NFPA Rating:
Ethanol: Health: 0
Fire: 3
Reactivity: 0
Extinguisher Media:
Use dry chemical, CO2 or appropriate foam.
Flammable Limits in Air % by Volume: Ethyl alcohol: LEL 3.3%, UEL-19%
Autoignition Temperature: Ethyl alcohol-793F
Special Firefighting Procedures:
Firefighters should wear full protective equipment and NIOSH approved
self-contained breathing apparatus.
Unusual Fire and Explosion Hazards: Extremely flammable. Vapors are
heavier
than air and can travel distances to ignition source and flash back.
6. SPILL OR LEAK PROCEDURES
Steps to be Taken in Case Material is Released or Spilled:
Ventilate area of spill. Eliminate all sources of ignition.
Remove all non-essential personnel from area. Clean-up personnel should
wear proper protective equipment and clothing. Absorb material with
suitable absorbent and containerize for disposal.
7. SPECIAL PRECAUTIONS
Precautions to be Taken in Handling or Storing: This material should be
kept
in an area suitable for the storage of flammable liquids. Bond and
ground
containers when transferring liquid.
Other Precautions: Keep oxidizing materials and strong acids away.
Wear
splash-proof chemical safety goggles and rubber gloves at all times
during
cleanup process.
8. SPECIAL PROTECTION INFORMATION
Respiratory Protection(Specify Type):
A NIOSH/MSHA chemical cartridge respirator should be worn if PEL or
TLV is exceeded.
Ventilation:
Local Exhaust: Yes
Mechanical(General):Yes
Special: No
Other: No
Protective Gloves:
Rubber, neoprene, PVC, or equivalent.
Eye Protection:
Splash proof chemical safety goggles should be worn at all times.
Other Protective Clothing or Equipment:
Lab apron, eye wash, and safety shower.
9. PHYSICAL DATA
Molecular Weight: No information available
Melting Point: No information available
Page 2 of 4
MATERIAL SAFETY DATA SHEET
Revised: 16/07/04
PHLOROGLUCINOL STAINING SOLUTION Replaces: 09/05/00
CAROLINA BIOLOGICAL Printed: 20/05/08


Boiling Point: No information available
Vapor Pressure: No information available
Vapor Density(Air=1): No information available
Specific Gravity(H2O=1): Less than 1
Percent Volatile by Volume: 100%
Evaporation Rate(H2O=1): Greater than 1
Solubility in Water: 100%
Appearance and Odor: Clear liquid, fruity odor
10. REACTIVITY DATA
Stability: Stable
Conditions to Avoid: Heat, sparks, open flame
Incompatibility(Materials to Avoid): Strong oxidizing agents.
Hazardous Decomposition Products: Carbon dioxide. Carbon monoxide
Hazardous Polymerization: Will not occur
11. TOXICITY DATA
Toxicity Data: To the best of our knowledge, the toxicological
properties of
this mixture have not been thoroughly evaluated. Data is listed for
individual components.
Ethyl alcohol: orl-rat LD50: 7.060 mg/kg. ihl-rat LC50: 20,000 ppm/10H
Methyl isobutyl ketone: orl-rat LD50: 5.080 mg/kg. ihl-rat LC50: 8.000
ppm/4H
Hexane: orl-rat LD50: 28.710 mg/kg, ihl-hmn TCLo: 5.000 ppm/10M
Phloroglucinol: sub-rat TDlo: 5mg/kg
Effects of Overexposure:
Acute: See section 3
Chronic: Ethyl alcohol: Mutation data cited. Reproductive
effects
data cited. Turmorigenic data cited. Not listed as causing cancer by
IARC,
NTP, or OSHA.
Methyl isbutyl ketone: No chronic effects data found. Not listed as
causing
cancer by IARC, NTP, or OSHA.
Hexane: Mutation data cited. Reproductive effects data cited. Not
listed as
causing cancer by IARC, NTP, or OSHA.
Phloroglucinol: Mutation and reproductive data cited. Not listed as
causing
cancer by NTP, IARC or OSHA.
Conditions Aggravated by Overexposure: Pre-existing conditions of the
skin,
eyes, throat, liver.
Target Organs: Skin, eyes, nose, throat
Primary Route(s) of Entry: Ingestion, inhalation
12. ECOLOGICAL DATA
EPA Waste Numbers: Methyl isobutyl ketone (U161) is considered a
hazardous
waste if and when it is discarded.
13. DISPOSAL INFORMATION
Waste Disposal Methods: Dispose in accordance with all applicable
Page 3 of 4
MATERIAL SAFETY DATA SHEET
Revised: 16/07/04
PHLOROGLUCINOL STAINING SOLUTION Replaces: 09/05/00
CAROLINA BIOLOGICAL Printed: 20/05/08


Federal,
State and Local regulations.
Always contact a permitted waste disposer (TSD) to assure compliance.
14. TRANSPORT INFORMATION
Description: Flammable liquids, n.o.s.(ethanol, methyl isobutyl ketone,
hexane), 3, UN1993, II
15. REGULATORY INFORMATION
EPA TSCA Status: On TSCA Inventory
Hazard Category for SARA Section 311/312 Reporting: Acute

SARA Sec. 313
SARA EHS Chemicals CERCLA RCRA
Product or Sec. 302 Name Chemical Sec. 103 Sec.
Components TPQ List Category RQ lbs. 261.33
_________________________________________________________________
Ethyl alcohol No No No No No
Methyl isobutyl
ketone No Yes No 5000 Yes
Hexane No No No No No
Phloroglucinol No No No No No
16. ADDITIONAL INFORMATION
The information provided in this Material Safety Data Sheet represents a
compilation of data drawn directly from various sources available to us.
Carolina Biological Supply makes no representation or guarantee as to
the suitability of this information to a particular application of the
substance covered in the Material Safety Data Sheet. Any employer must
carefully assess the applicability of any information contained herein
in regards to the particular use to which the employer puts the
material.
Glossary
ACGIH.......American Conference of Governmental Industrial Hygienists
CAS Number..Chemical Abstracts Service Number
CERCLA......Comprehensive Environmental Response, Compensation, and
Liability Act
DOT.........U.S. Department of Transportation
IARC........International Agency of Research on Cancer
mppcf......million particles per cubic foot
N/A.........Not Available
NTP.........National Toxicology Program
OSHA........Occupational Safety and Health Administration
PEL.........Permissible Exposure Limit
ppm.........parts per million
RCRA........Resource Conservation and Recovery Act
SARA........Superfund Amendments and Reauthorization Act
TLV.........Threshold Limit Value
TSCA........Toxic Substances Control Act




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