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12065 | Macrophage SFM
PRODUCT DESCRIPTION
A serum free medium designed for the culture of human monocytes and macrophages.
CATALOG NUMBERS
BRAND
LABELING INTENDED USE
STABILITY
STORAGE CONDITIONS
SHIPPING CONDITIONS
QC SPECIFICATION
PROTOCOLS AND APPLICATION NOTES
FORMULATION
PRODUCT DOCUMENTATION
FAQ
REFERENCES
CATALOG NUMBER
12065-074 500mL
BRAND
GIBCO庐
LABELING INTENDED USE
For Research Use Only
STABILITY
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12 months from date of production.
STORAGE CONDITIONS
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This product should be stored at 2-8潞C, in the dark.
SHIPPING CONDITIONS
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This product is shipped at room temperature.
QC SPECIFICATIONS
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Test Result
1
Sterility Testing NEGATIVE
Mycoplasma Testing NEGATIVE
pH 6.8 to 7.3
Osmolality 310 to 340 mOsm/kg
Endotoxin Testing NMT 1 EU/ml
Sp2 Toxicity 80 to 200%
Hepatitis B Surface Antigen Screening Non-reactive*
HIV 1 & 2 Antibody Screening Non-reactive*
HCV Antibody Screening Non-reactive*
*performed on the raw component prior to use in manufacturing
PROTOCOL AND APPLICATION NOTES
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Cell Lines Successfully Cultivated:
Lymphocytes, monocytes, macrophages, large granular lymphocytes, melanoma.
Historical Information:
The ability of isolated human monocytes/macrophages to lyse human targets effectively is used to assess macrophage-SFM
medium performance. Monocytes are isolated by discontinuous density gradient centrifugation followed by adherence to plastic
culture plates. The adherent cells are activated for 72 hours in macrophage-SFM in the presence of human rIFN-gamma and LPS.
Lytic activity is determined by incubating 51-Cr labeled U937 target cells with the activated monocytes/macrophages for 18 hours.
The relative cytotoxicity of the activated effectors is measured by determining the resulting release of 51-Cr from the U937 target
cells. Monocyte performance in each newly manufactured lot of macrophage-SFM must compare in an acceptable manner to a
previously approved reference lot.
FORMULATION
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Contact Gibco Technical Services with specific questions.
PRODUCT DOCUMENTATION
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Brochures Cell lines Citations
COA FAQ鈥檚 Manual
MSDS
REFERENCES
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1. Lacerna, L.V., Sugarbaker, P.H., and Stevenson, H.C. (1989) in Adoptive Cellular Immunotherapy of Cancer. (ed. H.C.
Stevenson) Marcel Dekker, NY, pp127.
2. Andreesen, R., Scheibenbogen, C., Brugger, W., et al., (1990) Cancer Res., 50:7450.
3. Dumont, S., Hartmann, D., Poindron, P., et al., (1988) Eur. J. Cancer Clin. Oncol., 24:1691.
4. Boyum, A. (1974) Tissue Antigens 4:269.
5. Mosmann, T. (1983) J. Immunol. Meth., 65:55.
6. Harmsen, A.G., Muggenburg, B.A., Snipes, M.B., et al., (1985) Science 230:1221.
7. Yanelli, J.R., Jadus, M.R., Beckner, S., et al., (1989) in Adoptive Cellular Immunotherapy of Cancer. (ed. H.C.
Stevenson) Marcel Dekker, NY, p191.
8. Metchnikoff, E. (1905) Immunity in Infective Diseases. Cambridge University Press, Cambridge, England.
9. Bonta, I.L. and Ben-Afraim, S. (1990) Immunol. Letters 25: 295.
10. DeWeger, R.A., Runhaar, B.A., and Den Otter, W. (1986) Methods Enzymol., 132:458.
11. Le, J., Prensky, W., Yip, Y.K., et al., (1983) J. Immunol., 131:2821.
12. Mufson, R.A., Aghajanian, J., Wong, G., et al., (1989) Cell Immunol., 119:182.
13. Keller, R., Keist, R., Wechsler, A., et al., (1990) Int. J. Cancer 46:682.
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14. Onozaki, K., Matsushima, K., Kleinerman, E.S., et al., (1985) J. Immunol., 135:314.
15. Malkovsky, M., Loveland, B., North, M., et al., (1987) Nature 325:262.
16. Meltzer, M.S., Crawford, R.M., Finboom, D.S., et al., (1987) Lymphokine Res., 6:1719.
17. Kirsh, R., Johnson, W.J., Bugelski, P.J., et al., (1988) in Macrophages and Cancer (eds. Heppner, G.H. and A.M. Fulton)
CRC Press, Boca Raton, Florida, pp169.
18. Gartner, S. and Popovic, M. (1990) AIDS Res. Hum. Retrovir., 6:1017.
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