MATERIAL SAFETY DATA SHEET



1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION



Product:         DNAzol®, DNAzol ES (Extra Strength), DNAzol BD (Blood)

Cat No:          DN-127, DN-128, DN-129



Molecular Research Center, Inc.

5645 Montgomery Rd.

Cincinnati, Ohio 45212

1-888-841-0900



Product Name: DNAzol Reagent (Genomic DNA Isolation Reagent)

Chemical Formula: A formulation

Molecular Weight: A formulation



NFPA RATINGS: (scale 0-4)           Health=1         Fire=0 Reactivity=0

HMIS RATINGS: (scale 0-4)           Health=1         Fire=0 Reactivity=0        PPE=C



CHEMTREC EMERGENCY NUMBER: 1-703-527-3887



2. COMPOSITION AND INFORMATION ON INGREDIENTS



Component                                   CAS No.           RTECS #           Percent

Guanidine Thiocyanate                       593-84-0          XL 1225000        Proprietary Information

Nonhazardous Ingredients                    N/A               N/A               Proprietary Information





3. HEALTH HAZARD IDENTIFICATION



ROUTES OF ENTRY:

                           Inhalation:      Yes

                           Skin:            Yes

                           Ingestion:       Yes



EFFECTS OF OVEREXPOSURE: A mildly hazardous product, which can irritate skin and eyes and is expected to have

moderate inhalation or ingestion toxicity. May produce hazardous vapors, including nitrous oxide and cyanide, if heated to

decomposition. To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly

investigated.



ACUTE EFFECTS



EYE EXPOSURE: May cause reddening and irritation of the eye.



SKIN OVEREXPOSURE: May cause irritation to skin consisting of reddening, itching, and pain.



INHALATION: May cause irritation of the throat, coughing, shortness of breath and respiratory tract irritation.



INGESTION: May be harmful if swallowed causing psychosis, vomiting, disorientation, weakness, low blood pressure, and

convulsions.





4. FIRST AID



FIRST AID FOR EYES: Check for and remove contact lenses. Flush with water in an eyewash for at least 15 minutes,

holding eyelids open. Do not use eye ointment. Obtain medical attention if discomfort or medical symptoms persist.

FIRST AID FOR SKIN: Remove contaminated clothing and wipe off excess solution from skin. Flush area with water for

10-15 min. Use deluge safety shower to decontaminate large areas of body surface with running water and nonabrasive soap.

Obtain medical attention if discomfort or symptoms persist.



FIRST AID FOR INHALATION: Remove person to fresh air. Monitor for respiratory distress and start artificial

respiration, if needed. Obtain medical attention if discomfort or symptoms persist.



FIRST AID FOR INGESTION: Remove dentures, if any. Have conscious person drink several glasses of water or milk.

Induce vomiting by sticking finger in throat. Lower head so vomit will not reenter the mouth or throat. Never give an

unconscious person anything to ingest. Obtain medical attention.





5. FIRE AND EXPLOSION HAZARD DATA



Combustible: Yes

Flash point: 59 C (D93 Method A)

Autoignition temperature: N/A

Flammable limits in air by volume, percent: lower: N/A; Upper: N/A

Transportation of dangerous goods flammability class: Combustible



EXTINGUISHING MEDIA: Product may contribute to a fire at elevated temperatures or by contact with an ignition

source. Use water spray, CO2, dry chemical powder, alcohol or polymer foam.



FIREFIGHTING PROCEDURES: Wear positive pressure, self-contained breathing apparatus and protective clothing.

Prevent contact with skin and eyes. Unusual fire and explosion hazards: emits toxic fumes under fire conditions, including

carbon and nitrogen oxides, sulfur oxides, hydrogen cyanide and ammonia.





6. ACCIDENTAL RELEASE MEASURES



Note that accidental releases may be subject to special reporting requirements and other regulatory mandates. Check and

comply with applicable laws and regulations.



PERSONAL PROTECTIVE EQUIPMENT FOR SPILL CONDITIONS: Use gloves, boots, and impermeable clothing

to avoid skin contact. Use chemical goggles, face shield, or other appropriate eye protection.



SPILL AND LEAK RESPONSE: Evacuate area, allowing trained personnel to use pre-planned procedures to perform

cleanup. Wear personal protective equipment as required. Remove all sources of ignition. Contain liquid with absorbant

material. Decontaminate and ventilate area. Place spill residue in sealed double bags. Dispose of contaminated material in

accordance with federal, state and local hazardous waste disposal regulations.





7. HANDLING AND STORAGE



IRRITANT. Keep container tightly closed in a cool, dry area. Avoid contact with acids. Avoid skin contact and inhalation.

Wear eye protection and gloves when handling. Store reagent at room temperature (15-30 C), under standard atmospheric

conditions. Keep container tightly sealed to minimize evaporation. The solution is suitable for most laboratory storage areas.

Avoid eating and smoking in work areas. Wash hands thoroughly after using this material.





8. EXPOSURE CONTROLS / PERSONAL PROTECTION



GENERAL PROTECTION AND PRECAUTIONS

Laboratory exhaust hood or local exhaust should be used when working with large volumes of reagent. Ensure availability of

safety shower and eyewash before using.



ROUTINE OPERATIONS: For routine use, latex gloves, chemical safety goggles, and lab coat are considered the

minimum body protection. Avoid generation of aerosols. Wash hands thoroughly after handling.

9. PHYSICAL AND CHEMICAL PROPERTIES



Appearance and odor:              either a green or amber liquid solution with a medicinal-like odor.

Vapor density:                    N/A

Evaporation rate:                 N.D.A.

Melting point:                    N/A

Boiling point:                    N.D.A.

Specific gravity:                 >1

Vapor press.mmHG@20C              N.D.A.

pH                                ~ 8.5





10. STABILITY AND REACTIVITY



STABILITY: Stable under normal conditions of use, temperature and pressure.



INCOMPATIBILITIES: Strong oxidizing agents, iron, strong acids, and acid chlorides.



HAZARDOUS COMBUSTION OR DECOMPOSITION PRODUCTS: Carbon monoxide, carbon dioxide, nitrogen

oxides, sulfur oxides, and cyanide.



CONDITIONS TO AVOID: Heat, flames, ignition sources and incompatibilities.





11. TOXICOLOGICAL INFORMATION



TOXICITY DATA: Mouse:(guanidine isothiocyanate) IP LD50=300 mg/kg, (thiocyanate) oral LD50=362 mg/kg. Rat:

(thiocyanate) oral LD50=764 mg/kg. Human: No info found. Causes eye and skin irritation. Material is an irritant to

mucous membranes and upper respiratory tract. Only selected data is presented here. To the best of our knowledge, all the

properties of this compound have not been investigated thoroughly. See actual entry in Registry of Toxic Effects of

Chemical Substances (RTECS# X11225000) for complete information.





12. ECOLOGICAL INFORMATION



ENVIRONMENTAL FATE: Structure suggests material is biodegradable in moist soil or water, expected to rapidly

decompose in the environment. Ecotoxicological information: Little or no toxic effects found.





13. DISPOSAL CONSIDERATIONS



EPA Waste Number (RCRA Hazard Class): Not specifically listed in the D, F, K, P, or U waste tables of the U.S. EPA

Resource Conservation and Recovery Act, 40 CFR Part 261. Note that treatment and disposal activities may be subject to

laws and regulations that may include before-the-fact permitting as well as reporting requirements. It is the purchaser’s

responsibility to comply.



DISPOSAL BY INCINERATION: Keep in sealed containers between use and final disposal. May be incinerated in a

hazardous waste incinerator equipped with appropriate controls for oxides of sulfur and nitrogen. All waste disposal

activities are subject to federal, state and local laws and regulations.



DISPOSAL IN WASTE WATER: Low concentrations are expected to degrade in biological wastewater treatment

facilities.





14. TRANSPORT INFORMATON



This material is not a hazardous material as defined by 49 CFR 172.101 by the U.S. DOT. (Guanidine isothiocyanate)

15. REGULATORY INFORMATION



Note that it is the responsibility of the purchaser and of those handling this material to comply with applicable laws and

regulations that are site and activity specific.



OSHA: CONSIDERED HAZARDOUS UNDER 29 CFR 1910.1200: NO PEL ESTABLISHED.

TSCA INVENTORY STATUS: INCLUDED IN THE US EPA TSCA: 40 CFR PART 710.

SARA: NO RQ LISTED.

CANADIAN REGULATIONS: WORKPLACE HAZARDOUS MATERIALS INFORMATION SYSTEM: THIS

PRODUCT HAS BEEN CLASSIFIED ACCORDING TO THE HAZARD CRITERIA OF THE CONTROLLED

PRODUCTS REGULATIONS, AND THE MSDS CONTAINS ALL REQUIRED INFORMATION.

EUROPEAN UNION: NOT FOUND

RISK PHRASE: WARNING!

R20/21/22: HARMFUL BY INHALATION, IN CONTACT WITH SKIN AND IF SWALLOWED.

SAFETY PHRASE: S24/25 AVOID CONTACT WITH SKIN AND EYES.

R32: CONTACT WITH ACIDS LIBERATES VERY TOXIC GAS.

R36/37/38: IRRITATING TO EYES, RESPIRATORY SYSTEM AND SKIN.

SAFETY SYMBOL: HARMFUL





16. OTHER INFORMATION                        Reviewed by          BW, MJ

                                             Revision date        5/01/07



This information is believed to be accurate and represents the information currently available to us. However, we make no

warranty of merchantability or any other warranty, expressed or implied, with respect to such information, and we assume no

liability resulting from its use. Users should make their own investigations to determine the suitability of the information for

their particular purposes.



Abbreviations:

N/A                                                      Not Applicable

N.D.A                                                    No data available