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Material Safety Data Sheet
Printing date 10/17/2007 Reviewed on 10/08/2007
1 Identification of substance:
路 Product details:
路 Trade name: 50X Tris/Acetic Acid/EDTA Buffer
路 Catalog or product number: 1610743, 1610773, 9704629, 9703685, 9702659, 1660742, 1660742EDU, 1610743EDU, 1610773EDU
路 Application of the substance / the preparation Laboratory chemicals
路 Manufacturer/Supplier:
Bio-Rad Laboratories, Life Science Group
2000 Alfred Nobel Drive
Hercules, California 94547 (510)741-1000
路 Information department: Technical services, customer support.
路 Emergency information:
1(800)424-9300 Use only in the event of a CHEMICAL EMERGENCY involving a SPILL, LEAK, FIRE, EXPLOSION or ACCIDENT.
510-741-1000
2 Composition/Information on components:
路 Chemical characterization
路 Description: Mixture of the substances listed below with non-hazardous additions.
路 Listing of dangerous and non-hazardous components:
CAS: 7732-18-5 water 50-100%
EINECS: 231-791-2
RTECS: ZC 0110000
CAS: 77-86-1 Tris(hydroxymethyl)aminomethane Xn, Xi; R 22-36 20-35%
EINECS: 201-064-4
RTECS: TY 2900000
CAS: 127-09-3 sodium acetate 10-20%
EINECS: 204-823-8
CAS: 6381-92-6 Ethylenediaminetetraacetic acid disodium salt dihydrate Xn, Xi; R 22-36/37/38 1.0-2.5%
RTECS: AH 4375000
路 Additional information For the wording of the listed risk phrases refer to section 15.
3 Hazards identification
路 Emergency overview:
Xn Harmful
路 Routes of exposure: Ingestion
路 Information pertaining to particular dangers for man and environment
The product has to be labelled due to the calculation procedure of the "General Classification guideline for preparations of the EU" in
the latest valid version.
R 22 Harmful if swallowed.
路 Classification system
The classification was made according to the latest editions of the EU-lists, and expanded upon from company and literature data.
4 First aid measures
路 General information
Symptoms of poisoning may even occur after several hours; therefore medical observation for at least 48 hours after the accident.
路 After inhalation Supply fresh air; consult doctor in case of complaints.
(Contd. on page 2)
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Page 2/5
Material Safety Data Sheet
Printing date 10/17/2007 Reviewed on 10/08/2007
Trade name: 50X Tris/Acetic Acid/EDTA Buffer
(Contd. of page 1)
路 After skin contact Generally the product does not irritate the skin.
路 After eye contact Rinse opened eye for several minutes under running water. Then consult a doctor.
路 After swallowing Immediately call a doctor.
5 Fire fighting measures
路 Suitable extinguishing agents CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
路 Protective equipment: No special measures required.
6 Accidental release measures
路 Person-related safety precautions: Not required
路 Measures for environmental protection:
Dilute with plenty of water.
Do not allow to enter sewers/ surface or ground water.
路 Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Dispose contaminated material as waste according to item 13.
7 Handling and storage
路 Handling
路 Information for safe handling: No special precautions are necessary if used correctly.
路 Information about protection against explosions and fires: No special measures required.
路 Storage
路 Requirements to be met by storerooms and receptacles: According to product specification
路 Information about storage in one common storage facility: Not required.
路 Further information about storage conditions: None.
8 Exposure controls and personal protection
路 Additional information about design of technical systems: No further data; see item 7.
路 Components with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at the workplace.
路 Additional information: The lists that were valid during the creation were used as basis.
路 Personal protective equipment
路 General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
Wash hands before breaks and at the end of work.
路 Breathing equipment: Not required.
路 Protection of hands: Protective gloves.
路 Material of gloves
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and varies from
manufacturer to manufacturer. As the product is a preparation of several substances, the resistance of the glove material can not be
calculated in advance and has therefore to be checked prior to the application.
Synthetic gloves
(Contd. on page 3)
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Material Safety Data Sheet
Printing date 10/17/2007 Reviewed on 10/08/2007
Trade name: 50X Tris/Acetic Acid/EDTA Buffer
(Contd. of page 2)
路 Penetration time of glove material
The exact break through time has to be found out by the manufacturer of the protective gloves and has to be observed.
路 Eye protection: Safety glasses
* 9 Physical and chemical properties:
路 General Information
liquid
Form:
Colorless
Color:
Odorless
Odor:
路 Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: 100掳C
Not applicable
路 Flash point:
Product is not selfigniting.
路 Auto igniting:
Product does not present an explosion hazard.
路 Danger of explosion:
23 hPa
路 Vapor pressure at 20掳C:
Not determined
路 Density:
路 Solubility in / Miscibility with
Fully miscible
Water:
7.1
路 pH-value at 20掳C:
路 Solvent content:
0.0 %
路 Organic solvents:
60.3 %
路 Water:
39.7 %
路 Solids content:
10 Stability and reactivity
路 Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
路 Dangerous reactions No dangerous reactions known
路 Dangerous products of decomposition: No dangerous decomposition products known
11 Toxicological information
路 Acute toxicity:
路 LD/LC50 values for hazardous components per OSHA criteria:
77-86-1 Tris(hydroxymethyl)aminomethane
Oral LD50 5900 mg/kg (rat)
127-09-3 sodium acetate
Oral LD50 3530 mg/kg (rat)
路 Primary irritant effect:
路 on the skin: No irritant effect.
(Contd. on page 4)
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Material Safety Data Sheet
Printing date 10/17/2007 Reviewed on 10/08/2007
Trade name: 50X Tris/Acetic Acid/EDTA Buffer
(Contd. of page 3)
路 on the eye: No irritant effect.
路 Sensitization: No sensitizing effects known.
路 Additional toxicological information:
The product shows the following dangers according to the calculation method of the General EU Classification Guidelines for
Preparations as issued in the latest version:
Harmful
12 Ecological information:
路 General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.
13 Disposal considerations
路 Product:
路 Recommendation
Hand over to hazardous waste disposers.
Must not be disposed of together with household garbage. Do not allow product to reach sewage system.
路 Uncleaned packagings:
路 Recommendation: Disposal must be made according to official regulations.
路 Recommended cleansing agent: Water, if necessary with cleansing agents.
14 Transport information
路 DOT regulations:
-
路 Hazard class:
路 Land transport ADR/RID (cross-border)
路 ADR/RID class: -
路 Maritime transport IMDG:
-
路 IMDG Class:
路 Marine pollutant: No
路 Air transport ICAO-TI and IATA-DGR:
路 ICAO/IATA Class: -
* 15 Regulations
路 SARA (Superfund Amendents and Reauthorization Act of 1986 - USA)
路 Section 302/304 (40CFR355.30 / 40CFR355.40):
None of the ingredients is listed.
路 Section 313 (40CFR372.65):
None of the ingredients is listed.
路 TSCA (Toxic Substances Control Act):
7732-18-5 water
(Contd. on page 5)
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Material Safety Data Sheet
Printing date 10/17/2007 Reviewed on 10/08/2007
Trade name: 50X Tris/Acetic Acid/EDTA Buffer
(Contd. of page 4)
77-86-1 Tris(hydroxymethyl)aminomethane
127-09-3 sodium acetate
路 Cancerogenity categories
路 EPA (Environmental Protection Agency)
None of the ingredients is listed.
路 IARC (International Agency for Research on Cancer)
None of the ingredients is listed.
路 NTP (National Toxicology Program)
None of the ingredients is listed.
路 TLV (Threshold Limit Value established by ACGIH)
None of the ingredients is listed.
路 MAK (German Maximum Workplace Concentration)
None of the ingredients is listed.
路 NIOSH-Ca (National Institute for Occupational Safety and Health)
None of the ingredients is listed.
路 OSHA-Ca (Occupational Safety & Health Administration)
None of the ingredients is listed.
路 Markings according to EU guidelines:
The product has been classified and marked in accordance with EU Directives / Ordinance on Hazardous Materials
路 Code letter and hazard designation of product:
Xn Harmful
路 Risk phrases:
22 Harmful if swallowed.
路 Safety phrases:
26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
36 Wear suitable protective clothing.
60 This material and its container must be disposed of as hazardous waste.
路 National regulations
路 Water hazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.
16 Other information:
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and
shall not establish a legally valid contractual relationship.
路 Department issuing MSDS: Environmental Health and Safety.
路 Contact:
Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000
Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000
路 * Data compared to the previous version altered.
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