MATERIAL SAFETY DATA SHEET-V4
Product NBT/BCIP substrate
Cat. no. R008R.0000
1. IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND OF THE COMPANY/UNDERTAKING
Identification of the product Produced and Marketed by
Name : NBT/BCIP substrate PanPath B.V.
Product code : R008R.0000 Science Park Amsterdam
Kruislaan 406, 1098 SM Amsterdam, the Netherlands
Tel +31 20 751 2030 - Fax +31 20 751 2031
2. COMPOSITION AND INFORMATION ON INGREDIENTS
- NBT (Molecular formula C40H30Cl2N10O6 in CsH7NO; Molecular weight 817.65)
- BCIP (Molecular formula C8H6NO4BrClP.C7H9N; Molecular weight 433.6)
Both ingredients are classified according to EEC Directive 88/379, the chemicals act and hazardous goods regulations as
hazardous.
- TRIS
- Dimethylformamide
- Sodium Azide (CAS-No. 26628-22-8; EINECS-No. 247-852-1; Molecular weight 65.01; Molecular formula N3Na; S: T+)
3. IDENTIFICATION OF HAZARDS AND EFFECTS
Conditions to avoid
Excess heat, flame or pressure.
Substances to avoid
N3Na liberates very toxic gas in contact with acids.
Hazardous combustion or decomposition products
Hydrochloric acid and nitric oxides.
Fire and explosion hazards
Inflammable. Emits toxic fumes under fire conditions. Keep away from inflammable sources.
4. FIRST AID MEASURES
EXPOSURE FIRST AID MEASURES
Inhalation Fresh air. Consult doctor if feeling unwell.
Eye contact Flush with copious amounts of water with eyelid held wide open. Summon eye specialist if necessary.
Skin contact Wash with copious amounts of water. Remove contaminated clothing.
Ingestion Summon doctor immediately! Watch out for vomiting: danger of breathing difficulties.
Keep bronchial tubes free.
5. FIRE FIGHTING MEASURES
Suitable extinguishing media : water spray, carbon dioxide, dry chemical powder or appropriate foam.
Special fire fighting procedure : not required.
PanPath B.V. - Tel.: +31 20 751 2030 - Fax: +31 20 751 2031 - info@panpath.nl - www.panpath.nl
PAGE 1 OF 4
MATERIAL SAFETY DATA SHEET-V4
Product NBT/BCIP substrate
Cat. no. R008R.0000
6. ACCIDENTAL RELEASE MEASURES
Take up with liquid-absorbent material, or absorb with fluid binders and dispose off suitably. Forward for disposal and clean up
affected area. Replace contaminated clothing immediately. There is no restriction of spilled substance. Consult a specialist for
disposal of the spilled substance.
7. HANDLING & STORAGE INFORMATION
Handling information
No special requirements.
Storage information
Store at 2-8 掳C.
8. PERSONAL PRECAUTIONS & PROTECTION
Personal precautions
The usual precautions taken when handling chemicals should be observed. Refer to product data sheet for recommended specific use.
Do not inhale vapors, do not get in eyes, on skin or clothing. Wash hands thoroughly after handling.
Personal protection
Respiratory protection : None required if ventilation is adequate.
Skin protection : Required. Wear suitable protective clothing and gloves.
Eye protection : Required. Wear safety glasses or goggles.
9. PHYSICAL AND CHEMICAL PROPERTIES
Appearance: liquid Flashing point: no data available
Color: reddish-brown Explosion limits
Odor: odorless 2.2 Vol%
lower limit:
pH at 200 g/l H2O (20 掳C): 4.5 鈥? 5.5 16.0 Vol%
upper limit:
Viscosity dynamic (20掳C): no data available Vapor pressure (25掳C):4 hpa
Boiling range: no data available Relative density: no data available
Melting point: no data available Solubility in water: completely
Autoflammability: no data available log P(o/w): no data available
10. STABILITY & REACTIVITY
Stability
There are no hazardous reactions when the substance is used appropriately. Hazardous decomposition products arise only in
small concentrations.
Reactivity
No data available
PanPath B.V. - Tel.: +31 20 751 2030 - Fax: +31 20 751 2031 - info@panpath.nl - www.panpath.nl
PAGE 2 OF 4
MATERIAL SAFETY DATA SHEET-V4
Product NBT/BCIP substrate
Cat. no. R008R.0000
11. TOXICOLOGICAL INFORMATION
Toxicological information
LD50 oral: 2800 mg/kg (rat) LD50 skin: 1400 mg/kg (rat)
LD50 oral: 3750 mg/kg (mouse) LD50 inhale: 2880 PPM (mouse)
Health effects (Acute and chronic)
The toxicological properties of this product have not been determined. It contains DMF which is a potent liver and kidney toxin. It may
be fetotoxic. Symptoms of exposure may include: irritation and nausea. Alcohol intolerance is possible and can last up to 4 days
following exposure. It contains NBT salts which can be irritating to the eyes, skin and respiratory tract. It also contains toluidine which
is suspected of being a cancer-causing agent. It may cause methemoglogin (reduced oxygen content in the body). Symptoms may
include grayish skin color, headache and dizziness.
Chronic effects
May cause birth defects.
.
12. ECOLOGICAL INFORMATION
Biologic degradation : no data available.
Behavior in environmental compartments : no data available.
Ecotoxic effects : substance is slighty hazardous to the water supply.
13. DISPOSAL CONSIDERATIONS
Product: there are no uniform EC regulations for the disposal of chemicals or residues. Chemical residues generally count as special
waste. The disposal of the latter is regulated in the EC member countries through corresponding laws and regulations. We recommend
that you contact either the authorities in charge or approved waste disposal companies which will advise you on how to dispose of
special waste.
Packaging: disposal in accordance with local provisions. Contaminated packages should be treated the same as the product itself.
14. TRANSPORT INFORMATION
Classification for transport by air/see: must be treated as hazardous goods according to current regulations for transportation of
Hazardous goods (GGVS/ADR, GGVE/RID, IMDG, IATA/ICAO)
15. REGULATORY INFORMATION
Labeling according to ECC directives.
R S SYMBOL
61-20/21-36 53-45 T
28-32-50/53 28-45-60-61 T+ N
36/38 Xi
34, 37
20/21/22 7/13, 2/13 Xn
Label precautionary statements: harmful by inhalation, in contact with skin and if swallowed. May cause birth defects. Irritating to
PanPath B.V. - Tel.: +31 20 751 2030 - Fax: +31 20 751 2031 - info@panpath.nl - www.panpath.nl
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MATERIAL SAFETY DATA SHEET-V4
Product NBT/BCIP substrate
Cat. no. R008R.0000
eyes, respiratory system and skin.
16. ADDITIONAL INFORMATION (IF APPLICABLE)
The information contained herein is based on the present state of our knowledge. It characterizes the product with regard to the
appropriate safety precautions. It does not represent a guarantee of the properties of the product. We make no warranty express or
implied, with respect to the information and assume no liability. Product users should determine the suitability of this information
for their own uses.
PanPath B.V. - Tel.: +31 20 751 2030 - Fax: +31 20 751 2031 - info@panpath.nl - www.panpath.nl
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