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File Name: promega_com---E1911AUS.asp
10/31/2007                                          Kit ingredients
Product: DLRâ„? Special Offer
Product code: E1911
Substance number Description Count Symbols
E624 phRL-TK Vector 1 -
E193 Stop & Glo® Substrate 1 Xn
E194 Passive Lysis 5X Buffer 1 -
E195 Luciferase Assay Buffer II 1 -
E197 Stop & Glo® Buffer 1 -
E463 Stop & Glo® Substrate Solvent 1 Xi F
E151 Luciferase Assay Substrate 1 -
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Not classified as hazardous according to criteria of Worksafe Australia


1 Identification of substance
Trade name: phRL-TK Vector
Article number: E624
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
37 Nelson Street
Annandale NSW 2038
AUSTRALIA
Sydney
Tel: (61) 02 9565 1100
Fax: (61) 02 9550 4454
Toll free Tel: 1800 225 123
Toll free Fax: 1800 626 017
Melbourne
Tel: (61) 03 9818 1466
Fax: (61) 03 9818 1244
Toll free Tel: 1800 335 435
E-mail Address: aus_custserv@au.promega.com
Web Address: www.promega.com/au/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.
AUS
(Contd. on page 2)




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: phRL-TK Vector

(Contd. of page 1)


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components: Void
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: No special measures required.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
(Contd. on page 3)
AUS




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: phRL-TK Vector

(Contd. of page 2)
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 0°C (32°F)
Boiling point/Boiling range: 100°C (212°F)
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
1 g/cm³
Density at 20°C (68°F):
Solubility in / Miscibility with
Not miscible or difficult to mix
Water:
Solvent content:
0.0 %
Organic solvents:
98.7 %
Water:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
(Contd. on page 4)
AUS




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: phRL-TK Vector

(Contd. of page 3)
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes: Generally not hazardous for water.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.


14 Transport information
Land transport ADR/RID (cross-border)
ADR/RID class: -
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -



15 Regulations
Australian Inventory of Chemical Substances
7732-18-5 water, distilled, conductivity or of similar purity
1185-53-1 Tris(hydroxymethyl)aminomethane hydrochloride
6381-92-6 Ethylenediaminetetra-acetic acid, disodium salt
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
National regulations
Waterhazard class: Generally not hazardous for water.


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
(Contd. on page 5)
AUS




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: phRL-TK Vector

(Contd. of page 4)

Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
AUS




DR
Page 1/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Hazardous according to criteria of Worksafe Australia


* 1 Identification of substance
Trade name: Stop & Glo® Substrate
Article number: E193
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
37 Nelson Street
Annandale NSW 2038
AUSTRALIA
Sydney
Tel: (61) 02 9565 1100
Fax: (61) 02 9550 4454
Toll free Tel: 1800 225 123
Toll free Fax: 1800 626 017
Melbourne
Tel: (61) 03 9818 1466
Fax: (61) 03 9818 1244
Toll free Tel: 1800 335 435
E-mail Address: aus_custserv@au.promega.com
Web Address: www.promega.com/au/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


* 2 Hazards identification
Hazard description:


Xn Harmful


Information concerning to particular hazards to man and environment
The product has to be labelled due to the calculation procedure of the "General Classification guideline for
preparations of the EU" in the latest valid version.
Harmful by inhalation, in contact with skin and if swallowed.
(Contd. on page 2)
AUS




DR
Page 2/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Substrate

(Contd. of page 1)
Harmful: possible risk of irreversible effects through inhalation, in contact with skin and if swallowed.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components:
CAS: 67-56-1 methanol 5-10%
RTECS: PC 1400000
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information:
Symptoms of poisoning may even occur after several hours; therefore medical observation for at least 48 hours
after the accident.
After inhalation:
Supply fresh air. If required, provide artificial respiration. Keep patient warm. Consult doctor if symptoms
persist.
In case of unconsciousness place patient stably in side position for transportation.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: Call for a doctor immediately.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: Mount respiratory protective device.


* 6 Accidental release measures
Person-related safety precautions: Wear protective equipment. Keep unprotected persons away.
Measures for environmental protection: Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting:
Dispose contaminated material as waste according to item 13.
Ensure adequate ventilation.


* 7 Handling and storage
Handling
Information for safe handling:
Thorough dedusting.
Keep receptacles tightly sealed.
Ensure good ventilation/exhaustion at the workplace.
Information about fire - and explosion protection: Keep ignition sources away - Do not smoke.
(Contd. on page 3)
AUS




DR
Page 3/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Substrate

(Contd. of page 2)

Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: Keep receptacle tightly sealed.


* 8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
67-56-1 methanol
NES (Australia) Short-term value: 328 mg/m³, 250 ppm
Long-term value: 262 mg/m³, 200 ppm
Sk
PEL (USA) 260 mg/m³, 200 ppm
REL (USA) Short-term value: 325 mg/m³, 250 ppm
Long-term value: 260 mg/m³, 200 ppm
Skin
TLV (USA) Short-term value: 328 mg/m³, 250 ppm
Long-term value: 262 mg/m³, 200 ppm
Skin; BEI
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
Immediately remove all soiled and contaminated clothing
Wash hands before breaks and at the end of work.
Avoid contact with the eyes and skin.
Respiratory protection:
In case of brief exposure or low pollution use respiratory filter device. In case of intensive or longer exposure use
self-contained respiratory protective device.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Not required.


* 9 Physical and chemical properties
General Information
Solid.
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: undetermined
(Contd. on page 4)
AUS




DR
Page 4/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Substrate

(Contd. of page 3)

Boiling point/Boiling range: 64°C (147°F)
> 55°C (> 131°F)
Flash point:
455°C (851°F)
Ignition temperature:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
0.794 g/cm³
Density at 20°C (68°F):
Solubility in / Miscibility with
Soluble
Water:
Solvent content:
6.2 %
Organic solvents:
93.8 %
Solids content:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: Reacts with acids, alkalis and oxidizing agents
Dangerous decomposition products: Carbon monoxide and carbon dioxide


11 Toxicological information
Acute toxicity:
LD/LC50 values relevant for classification:
67-56-1 methanol
Oral LD50 5628 mg/kg (Rat)
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product shows the following dangers according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
Harmful


12 Ecological information
General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.


13 Disposal considerations
Product:
Recommendation
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
(Contd. on page 5)
AUS




DR
Page 5/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Substrate

(Contd. of page 4)

Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
Recommended cleansing agents: Water, if necessary together with cleansing agents.


* 14 Transport information
Land transport ADR/RID (cross-border)




6.1 (T9) Toxic substances.
ADR/RID class:
Danger code (Kemler): 60
3243
UN-Number:
II
Packaging group:
6.1
Hazard label
Description of goods: 3243 SOLIDS CONTAINING TOXIC LIQUID, N.O.S. (METHANOL)
Maritime transport IMDG:




6.1
IMDG Class:
3243
UN Number:
6.1
Label
II
Packaging group:
F-A,S-A
EMS Number:
No
Marine pollutant:
Proper shipping name: SOLIDS CONTAINING TOXIC LIQUID, N.O.S. (METHANOL)
Air transport ICAO-TI and IATA-DGR:




6.1
ICAO/IATA Class:
3243
UN/ID Number:
6.1
Label
II
Packaging group:
Proper shipping name: SOLIDS CONTAINING TOXIC LIQUID, N.O.S. (METHANOL)


* 15 Regulations
Australian Inventory of Chemical Substances
67-56-1 methanol
7647-01-0 hydrogen chloride
Labelling according to EU guidelines:
The product has been classified and marked in accordance with EU Directives / Ordinance on Hazardous
Materials
(Contd. on page 6)
AUS




DR
Page 6/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Substrate

(Contd. of page 5)

Hazard-determining components of labelling:
methanol
Risk phrases:
Harmful by inhalation, in contact with skin and if swallowed.
Harmful: possible risk of irreversible effects through inhalation, in contact with skin and if swallowed.
Safety phrases:
Keep container tightly closed and in a well-ventilated place.
Keep away from sources of ignition - No smoking.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
This material and its container must be disposed of as hazardous waste.
National regulations
Technical instructions (air):
Class Share in %
I 6.2

Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega Corporation
37 Nelson Street
Annandale NSW 2038, Australia
* Data compared to the previous version altered.
AUS




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Not classified as hazardous according to criteria of Worksafe Australia


1 Identification of substance
Trade name: Passive Lysis 5X Buffer
Article number: E194
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
37 Nelson Street
Annandale NSW 2038
AUSTRALIA
Sydney
Tel: (61) 02 9565 1100
Fax: (61) 02 9550 4454
Toll free Tel: 1800 225 123
Toll free Fax: 1800 626 017
Melbourne
Tel: (61) 03 9818 1466
Fax: (61) 03 9818 1244
Toll free Tel: 1800 335 435
E-mail Address: aus_custserv@au.promega.com
Web Address: www.promega.com/au/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.
AUS
(Contd. on page 2)




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Passive Lysis 5X Buffer

(Contd. of page 1)


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components:
CAS: 56-81-5 glycerol 50-75%
RTECS: MA 8050000
CAS: 16052-06-5 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid 1.0-5.0%
CAS: 123333-90-4 Trans-1,2-Diaminocyclohexane-N,N,N',N',-tetraacetic acid, monohydrate 1.0-5.0%
RTECS: AG5000000
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents: Use fire extinguishing methods suitable to surrounding conditions.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
(Contd. on page 3)
AUS




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Passive Lysis 5X Buffer

(Contd. of page 2)
Ingredients with limit values that require monitoring at the workplace:
56-81-5 glycerol
NES (Australia) 10 mg/m³
PEL (USA) 15* 5** mg/m³
*total dust **respirable fraction
TLV (USA) 10 ppm
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: 100°C (212°F)
> 100°C (> 212°F)
Flash point:
400°C (752°F)
Ignition temperature:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
Explosion limits:
0.9 Vol %
Lower:
Vapour pressure at 20°C (68°F): 0.1 hPa (0 mm Hg)
Not determined
Density:
Solubility in / Miscibility with
Not miscible or difficult to mix
Water:
7.9-8.1
pH-value at 20°C (68°F):
Solvent content:
62.6 %
Organic solvents:
(Contd. on page 4)
AUS




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Passive Lysis 5X Buffer

(Contd. of page 3)

30.6 %
Water:
6.8 %
Solids content:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.


14 Transport information
Land transport ADR/RID (cross-border)
ADR/RID class: -
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
(Contd. on page 5)
AUS




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Passive Lysis 5X Buffer

(Contd. of page 4)

Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -



15 Regulations
Australian Inventory of Chemical Substances
56-81-5 glycerol
7732-18-5 water, distilled, conductivity or of similar purity
16052-06-5 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid
1310-73-2 sodium hydroxide
8047-15-2 Saponins
1405-20-5 Polymyxin B Sulfate
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
Special labelling of certain preparations:
Safety data sheet available for professional user on request.
National regulations
Technical instructions (air):
Class Share in %
Water 30.6
NK 62.6

Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
AUS




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Not classified as hazardous according to criteria of Worksafe Australia


1 Identification of substance
Trade name: Luciferase Assay Buffer II
Article number: E195
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
37 Nelson Street
Annandale NSW 2038
AUSTRALIA
Sydney
Tel: (61) 02 9565 1100
Fax: (61) 02 9550 4454
Toll free Tel: 1800 225 123
Toll free Fax: 1800 626 017
Melbourne
Tel: (61) 03 9818 1466
Fax: (61) 03 9818 1244
Toll free Tel: 1800 335 435
E-mail Address: aus_custserv@au.promega.com
Web Address: www.promega.com/au/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.
AUS
(Contd. on page 2)




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Luciferase Assay Buffer II

(Contd. of page 1)


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components: Void
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting: Pick up mechanically.
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
Respiratory protection: Not required.
(Contd. on page 3)
AUS




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Luciferase Assay Buffer II

(Contd. of page 2)
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Not required.


9 Physical and chemical properties
General Information
Solid.
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 0°C (32°F)
Boiling point/Boiling range: 100°C (212°F)
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
1 g/cm³
Density at 20°C (68°F):
Solubility in / Miscibility with
Insoluble
Water:
0
pH-value at 20°C (68°F):
Solvent content:
0.0 %
Organic solvents:
97.8 %
Water:
3.0 %
Solids content:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
(Contd. on page 4)
AUS




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Luciferase Assay Buffer II

(Contd. of page 3)
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.
Rinse off of bigger amounts into drains or the aquatic environment may lead to decreased pH-values. A low pH-
value harms aquatic organisms. In the dilution of the use-level the pH-value is considerably increased, so that
after the use of the product the aqueous waste, emptied into drains, is only low water-dangerous.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.


14 Transport information
Land transport ADR/RID (cross-border)
ADR/RID class: -
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -



15 Regulations
Australian Inventory of Chemical Substances
All ingredients are listed.
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
National regulations
Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.
AUS
(Contd. on page 5)




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Luciferase Assay Buffer II

(Contd. of page 4)


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
AUS




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Not classified as hazardous according to criteria of Worksafe Australia


1 Identification of substance
Trade name: Stop & Glo® Buffer
Article number: E197
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
37 Nelson Street
Annandale NSW 2038
AUSTRALIA
Sydney
Tel: (61) 02 9565 1100
Fax: (61) 02 9550 4454
Toll free Tel: 1800 225 123
Toll free Fax: 1800 626 017
Melbourne
Tel: (61) 03 9818 1466
Fax: (61) 03 9818 1244
Toll free Tel: 1800 335 435
E-mail Address: aus_custserv@au.promega.com
Web Address: www.promega.com/au/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.
AUS
(Contd. on page 2)




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Buffer

(Contd. of page 1)


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components: Void
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
(Contd. on page 3)
AUS




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Buffer

(Contd. of page 2)
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: 100°C (212°F)
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
Not determined
Density:
Solubility in / Miscibility with
Not miscible or difficult to mix
Water:
Solvent content:
0.0 %
Organic solvents:
94.7 %
Water:
5.3 %
Solids content:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
(Contd. on page 4)
AUS




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Buffer

(Contd. of page 3)
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.


14 Transport information
Land transport ADR/RID (cross-border)
ADR/RID class: -
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -



15 Regulations
Australian Inventory of Chemical Substances
7732-18-5 water, distilled, conductivity or of similar purity
7447-40-7 potassium chloride
acetic acid
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
National regulations
Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.
AUS
(Contd. on page 5)




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Buffer

(Contd. of page 4)


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
AUS




DR
Page 1/7
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Hazardous according to criteria of Worksafe Australia


* 1 Identification of substance
Trade name: Stop & Glo® Substrate Solvent
Article number: E463
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
37 Nelson Street
Annandale NSW 2038
AUSTRALIA
Sydney
Tel: (61) 02 9565 1100
Fax: (61) 02 9550 4454
Toll free Tel: 1800 225 123
Toll free Fax: 1800 626 017
Melbourne
Tel: (61) 03 9818 1466
Fax: (61) 03 9818 1244
Toll free Tel: 1800 335 435
E-mail Address: aus_custserv@au.promega.com
Web Address: www.promega.com/au/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description:

Xi Irritant
F Highly flammable

Information concerning to particular hazards to man and environment
The product has to be labelled due to the calculation procedure of the "General Classification guideline for
preparations of the EU" in the latest valid version.
Highly flammable.
(Contd. on page 2)
AUS




DR
Page 2/7
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Substrate Solvent

(Contd. of page 1)
Irritating to eyes.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components:
CAS: 64-17-5 ethanol 75-100%
RTECS: KQ 6300000
CAS: 56-81-5 glycerol 20-25%
RTECS: MA 8050000
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact:
Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.


* 6 Accidental release measures
Person-related safety precautions: Wear protective equipment. Keep unprotected persons away.
Measures for environmental protection:
Prevent seepage into sewage system, workpits and cellars.
Dilute with plenty of water.
Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Ensure adequate ventilation.


* 7 Handling and storage
Handling
Information for safe handling:
Store in cool, dry place in tightly closed receptacles.
Ensure good ventilation/exhaustion at the workplace.
Prevent formation of aerosols.
No special precautions are necessary if used correctly.
(Contd. on page 3)
AUS




DR
Page 3/7
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Substrate Solvent

(Contd. of page 2)
Information about fire - and explosion protection:
Keep ignition sources away - Do not smoke.
Protect against electrostatic charges.
Storage
Requirements to be met by storerooms and receptacles: Store in a cool location.
Information about storage in one common storage facility: Not required.
Further information about storage conditions:
Keep receptacle tightly sealed.
Store in cool, dry conditions in well sealed receptacles.


* 8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
64-17-5 ethanol
NES (Australia) 1880 mg/m³, 1000 ppm
PEL (USA) 1900 mg/m³, 1000 ppm
REL (USA) 1900 mg/m³, 1000 ppm
TLV (USA) 1880 mg/m³, 1000 ppm
56-81-5 glycerol
NES (Australia) 10 mg/m³
PEL (USA) 15* 5** mg/m³
*total dust **respirable fraction
TLV (USA) 10 ppm
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
Immediately remove all soiled and contaminated clothing
Wash hands before breaks and at the end of work.
Avoid contact with the eyes.
Avoid contact with the eyes and skin.
Respiratory protection:
In case of brief exposure or low pollution use respiratory filter device. In case of intensive or longer exposure use
self-contained respiratory protective device.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Tightly sealed goggles.
AUS
(Contd. on page 4)




DR
Page 4/7
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Substrate Solvent

(Contd. of page 3)


* 9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: 78°C (172°F)
� 21°C (� 70°F)
Flash point:
400°C (752°F)
Ignition temperature:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
Product is not explosive. However, formation of explosive air/vapour mixtures
are possible.
Explosion limits:
0.9 Vol %
Lower:
15.0 Vol %
Upper:
Vapour pressure at 20°C (68°F): 59 hPa (44 mm Hg)
0.86 g/cm³
Density at 20°C (68°F):
Solubility in / Miscibility with
Fully miscible
Water:
Solvent content:
99.0 %
Organic solvents:
0.7 %
Water:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions:
Reacts with acids, alkalis and oxidizing agents
Reacts with alkaline metals
Reacts with inorganic acid chlorides
Dangerous decomposition products: Carbon monoxide and carbon dioxide


11 Toxicological information
Acute toxicity:
LD/LC50 values relevant for classification:
Oral LD50 7060 mg/kg (Rat)
64-17-5 ethanol
Oral LD50 7060 mg/kg (Rat)
Inhalative LC50/4 h 20000 mg/l (Rat)
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
(Contd. on page 5)
AUS


DR
Page 5/7
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Substrate Solvent

(Contd. of page 4)
Additional toxicological information:
The product shows the following dangers according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
Irritant


12 Ecological information
General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.


13 Disposal considerations
Product:
Recommendation
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
Recommended cleansing agents: Water, if necessary together with cleansing agents.


* 14 Transport information
Land transport ADR/RID (cross-border)




3 (F1) Flammable liquids.
ADR/RID class:
Danger code (Kemler): 33
1170
UN-Number:
II
Packaging group:
3
Hazard label
Description of goods: 1170 ETHANOL SOLUTION (ETHYL ALCOHOL SOLUTION)
Maritime transport IMDG:




3
IMDG Class:
1170
UN Number:
3
Label
II
Packaging group:
F-E,S-D
EMS Number:
No
Marine pollutant:
(Contd. on page 6)
AUS




DR
Page 6/7
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Substrate Solvent

(Contd. of page 5)

Proper shipping name: ETHANOL SOLUTION (ETHYL ALCOHOL SOLUTION)
Air transport ICAO-TI and IATA-DGR:




3
ICAO/IATA Class:
1170
UN/ID Number:
3
Label
II
Packaging group:
Proper shipping name: ETHANOL SOLUTION


* 15 Regulations
Australian Inventory of Chemical Substances
All ingredients are listed.
Labelling according to EU guidelines:
The product has been classified and marked in accordance with EU Directives / Ordinance on Hazardous
Materials
Risk phrases:
Highly flammable.
Irritating to eyes.
Safety phrases:
Keep container tightly closed and in a well-ventilated place.
Keep away from sources of ignition - No smoking.
Do not breathe gas/fumes/vapour/spray (appropriate wording to be specified by the manufacturer).
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
This material and its container must be disposed of as hazardous waste.
National regulations
Technical instructions (air):
Class Share in %
Water 0.7
NK 99.0

Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
(Contd. on page 7)
AUS




DR
Page 7/7
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Stop & Glo® Substrate Solvent

(Contd. of page 6)
Contact:
Promega Corporation
37 Nelson Street
Annandale NSW 2038, Australia
* Data compared to the previous version altered.
AUS




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Not classified as hazardous according to criteria of Worksafe Australia


1 Identification of substance
Trade name: Luciferase Assay Substrate
Article number: E151
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
37 Nelson Street
Annandale NSW 2038
AUSTRALIA
Sydney
Tel: (61) 02 9565 1100
Fax: (61) 02 9550 4454
Toll free Tel: 1800 225 123
Toll free Fax: 1800 626 017
Melbourne
Tel: (61) 03 9818 1466
Fax: (61) 03 9818 1244
Toll free Tel: 1800 335 435
E-mail Address: aus_custserv@au.promega.com
Web Address: www.promega.com/au/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.
AUS
(Contd. on page 2)




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Luciferase Assay Substrate

(Contd. of page 1)


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components: Void
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
(Contd. on page 3)
AUS




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Luciferase Assay Substrate

(Contd. of page 2)
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 0°C (32°F)
Boiling point/Boiling range: 100°C (212°F)
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
1 g/cm³
Density at 20°C (68°F):
Solubility in / Miscibility with
Not miscible or difficult to mix
Water:
0
pH-value at 20°C (68°F):
Solvent content:
0.0 %
Organic solvents:
98.6 %
Water:
1.0 %
Solids content:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
(Contd. on page 4)
AUS




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Luciferase Assay Substrate

(Contd. of page 3)
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.
Rinse off of bigger amounts into drains or the aquatic environment may lead to decreased pH-values. A low pH-
value harms aquatic organisms. In the dilution of the use-level the pH-value is considerably increased, so that
after the use of the product the aqueous waste, emptied into drains, is only low water-dangerous.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.


14 Transport information
Land transport ADR/RID (cross-border)
ADR/RID class: -
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -



15 Regulations
Australian Inventory of Chemical Substances
7732-18-5 water, distilled, conductivity or of similar purity
987-65-5 Adenosine 5'-(tetrahydrogen triphosphate), disodium salt
6381-92-6 Ethylenediaminetetra-acetic acid, disodium salt
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
(Contd. on page 5)
AUS




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 31.10.2007 Revision: 31.10.2007

Trade name: Luciferase Assay Substrate

(Contd. of page 4)

National regulations
Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
AUS




DR

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