MATERIAL SAFETY DATA SHEET



1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION



                 TRI Reagent®RT, TRI Reagent®RT Blood, TRI Reagent®RT Liquid Samples,

Product:

Cat. Nos:        RT 111, RB 211, RL 311



Molecular Research Center, Inc.

5645 Montgomery Rd.

Cincinnati, Ohio 45212

USA 1-888-841-0900



Product Name: Tri Reagent®RT

Synonym: Phenol solution

Chemical Formula: A formulation

Molecular Weight: A formulation



CERCLA RATINGS: (scale 0-3) Health=3                 Fire=1    Reactivity=0    Persistence=1

NFPA RATINGS: (scale 0-4)        Health=3            Fire=1    Reactivity=0

HMIS RATINGS: (scale 0-4)        Health=3            Fire=1    Reactivity=0    PPE=D*

* Use chemical fume hood or adequate ventilation.



CHEMTREC EMERGENCY NUMBER: Only in the event of an emergency involving a spill, leak, fire exposure or accident.

1-800-424-9300





2. COMPOSITION/INFORMATION ON INGREDIENTS



Component                          CAS No            Percent         RTECS #            DOT UN:

Phenol                             108-95-2          <50             SJ3325000          282155

Nonhazardous Ingredients           NA 0004           <20

Thiocyanate compounds              NA 0022           <30





3. HEALTH HAZARD IDENTIFICATION



EFFECTS OF OVEREXPOSURE: Prolonged exposure to phenol fumes or contact with skin can be hazardous and

emergency medical attention should be initiated.



ROUTES OF ENTRY:

Inhalation: Yes

Skin:       Yes

Ingestion:  Yes



INHALATION: (phenol) Corrosive, neurotoxin, highly toxic. May cause respiratory tract irritation, injury or arrest.



ACUTE EFFECTS: Inhalation may cause severe irritation of the mucous membranes, profuse sweating, headache, intense

thirst, nausea and vomiting, abdominal pain, diarrhea, salivation, cyanosis, and convulsions. Methemoglobinemia and

hemolysis have been reported occasionally. Death may occur from respiratory, circulatory or cardiac failure.



CHRONIC EFFECTS: Symptoms of chronic phenol poisoning may include vomiting, difficulty swallowing, diarrhea,

anorexia, headache, vertigo, muscle weakness and pain, mental disturbances, dark or “smokey� urine and possible skin

eruptions. Extensive damage to the liver and kidneys may be fatal.

SKIN CONTACT: (phenol) Corrosive, neurotoxin.

Dermal contact may irritate/inflame the skin, with burning sensation or localized loss of feeling. Skin is a principle route of

entry and toxic quantities may be rapidly absorbed. Skin exposure can cause severe chemical burns. On skin, there is an

initial local anesthesia followed with a white discoloration.



ACUTE EFFECTS: Contact may cause local anesthesia, skin necrosis, and burns. Phenol burns may be severe, but painless

due to damage to nerve endings. Vapors and liquids may be readily absorbed through the skin to cause systemic effects as

detailed in acute inhalation exposure.



CHRONIC EFFECTS: Prolonged exposure may cause dermatitis, and skin sensitization. Coma and death have been

reported following extensive skin exposure. Pathologic findings include congestion of the lungs, liver, spleen, and kidneys.

Symptoms of chronic phenol poisoning may occur as detailed in chronic inhalation exposure. Animal studies indicate phenol

may have tumor promoting and/or mutagenic capabilities.



EYE CONTACT: (phenol) Corrosive to tissue, may cause blindness.



ACUTE EFFECTS: May cause tearing, conjunctival swelling, loss of sensation and blurred vision.



CHRONIC EXPOSURE: Repeated or prolonged exposure to phenol vapors may cause corneal ulceration, permanent

damage or blindness.



INGESTION: (phenol) May cause severe burns to the mouth or throat. May cause severe abdominal burning sensation.





4. FIRST AID



INHALATION: Immediately remove individual from exposure site to fresh air. If breathing has stopped, give artificial

respiration. Maintain airway and blood pressure and administer oxygen if available. Treat symptomatically and supportively.

 Oxygen should be administration by qualified personnel. Get medical attention immediately.



SKIN CONTACT: Remove contaminated clothing and shoes immediately. Wash affected area with soap or mild detergent

and large amounts of water until no evidence of chemical remains. Use a deluge shower for at least 15 minutes. In case of

chemical burns, cover area with sterile, dry dressing, bandage securely, but not too tightly. Get medical attention

immediately.



EYE CONTACT: Wash eyes immediately with large amounts of water, holding upper and lower lids open. Get medical

attention immediately.



INGESTION: Wash mouth out if vomiting occurs. Seek immediate medical attention.

Note to attending physician: No known specific antidote. Areas of skin contact smaller than 100 cm 2 may cause a minor

health hazard. Systemic doses less than 1 gm may cause a minor health hazard although individual sensitivity may vary. For

ingestion exposure: give castor oil or other vegetable oil. Give charcoal slurry if conscious. Treat symptomatically. Observe

for 24 hrs. Be prepared for emergency cardiovascular intervention.





5. FIRE FIGHTING MEASURES



Moderate fire hazard when exposed to heat or flame. Vapor-air mixtures are explosive above flash point. Vapors are heavier

than air and may travel a considerable distance to a source of ignition and flash back. Fires involving phenol should be fought

upwind from the maximum distance possible. In the absence of a contained breathing device, emergency personnel should

stay away from low areas and ventilate closed spaces before entry.



EXTINGUISHING MEDIA: Use Class B extinguishers (oils, hydrocarbon liquids.) Dry chemical, carbon dioxide, halon,

water spray or standard foam (1987 Emergency Response Guidebook, DOT P 5800.4) for larger fires, use water spray, fog or

standard foam (1987 Emergency Response Guidebook, DOT P 5800.4).

FIREFIGHTING: Evacuate area. Wear positive pressure self-contained breathing apparatus. Extinguish fire using

indicated agents. Phenol is combustible and containers may explode in fire. Avoid breathing toxic fumes produced under fire

conditions.





6. ACCIDENTAL RELEASE MEASURES



Note that accidental releases may be subject to special reporting requirements and other regulatory mandates. Check and

comply with local applicable laws and regulations.



PERSONAL PROTECTIVE EQUIPMENT: Use gloves, boots, Tyvek suit or other impervious covering to avoid skin

contact. Use chemical goggles, face shield, or other appropriate eye protection.



SPILL AND LEAK PROCEDURES: Restrict persons not wearing protective equipment from area. Remove all ignition

sources. Neutralize spill with slaked lime, sodium bicarbonate or crushed limestone. Collect powdered material and deposit

in sealed containers and dispose of phenol as hazardous waste. Isolate area and deny entry.



U.S. DOT EMERGENCY GUIDE # 60

NORTH AMERICAN EMERGENCY RESPONSE GUIDEBOOK # 153





7. HANDLING AND STORAGE



Observe all Federal, state, and local regulations when storing or disposing of this substance. Store in an area appropriate for

flammables; a cool, dry, well-ventilated location, away from direct sunlight, heat or sources of ignition. Avoid contact with

hypochlorite, strong oxidizers such as chlorine and bromine.





8. EXPOSURE CONTROLS AND PERSONAL PROTECTION



The current OSHA permissible exposure limit (PEL) for phenol is 5 ppm (19 milligrams per cubic meter) as an 8-hour time-

weighted average (TWA) concentration.



GENERAL PROTECTION AND PRECAUTIONS





PROTECTIVE MEASURES: Do not touch unprotected skin. Do not wear contact lenses while handling this product. Do

not pipette by mouth. Area ventilation is generally adequate, but use fume hood if available.



AIR PURIFYING RESPIRATOR CANISTERS / CARTRIDGES: Stacked cartridge for organic vapors (black ANSI

color code, NIOSH approved) plus dust, mist (red ANSI color code, NIOSH approved).



GLOVES AND PROTECTIVE CLOTHING: Employee must wear appropriate (impervious) clothing and gloves (rubber

or neoprene rubber) to prevent any possibility of skin contact with this substance.



EYE PROTECTION: Safety glasses should be the minimum eye protection. Wear chemical goggles to reduce exposure to

aerosols or mists.



EMERGENCY WASH FACILITIES: Where there is any possibility that an employee's eyes and /or skin may be exposed

to this substance, the employer should provide an eye wash fountain and quick drench shower within the immediate work area

for emergency use.



ROUTINE OPERATIONS: Lab coats, safety glasses with side shields and gloves should be considered minimum body

protection. Wash hands thoroughly after using the reagent and never eat, drink, use tobacco products, apply cosmetics or take

medications in areas where a phenol solution is handled, processed or stored.

9. PHYSICAL AND CHEMICAL PROPERTIES

Description:        Red to maroon color with a characteristic sweet, medicinal or tar-like odor.

Boiling point:      Not determined

Melting point:      Not Applicable

Vapor pressure:     >0.35 mmHg@25C

Evaporation rate:   Not determined

Solvent solubility: Soluble in water and glycerol; relatively soluble in aqueous alkali hydroxides, and

dimethyl sulfoxide.





10. STABILITY AND REACTIVITY



STABILITY: Stable under normal temperatures and pressures.

INCOMPATIBILITIES: Acetaldehyde: violent reaction.

Aluminum and alloys: may corrode.

Aluminum chloride + nitrobenzene: violent explosion.

1,3-butadiene, boron trifluoride, and diethyletherate: possible explosion

Calcium hypochlorite: exothermic reaction with possible ignition.

Formaldehyde: possible exothermic reaction.

Lead and alloys: may corrode.

Magnesium and alloys: may corrode.

OXIDIZERS: (strong) Fire and explosion hazard.

Peroxodisulfuric acid: possible explosion.

Peroxomonosulfuric acid: explosion.

Plastics and rubber coatings: may corrode.

Sodium nitrate + trifluoroacetic acid: violent exothermic reaction.

Sodium nitrite: may explode.

Zinc and alloys: may corrode.

DECOMPOSITION: Thermal decomposition products may include toxic oxides of carbon.

Polymerization: Hazardous polymerization has not been reported to occur under normal temperatures and pressures.





11. TOXICOLOGICAL INFORMATION



TOXICITY DATA: Human: (phenol) 10 mg/kg oral-human LDLO. Rat: (phenol) 317 mg/ kg LD50 oral; TRI

Reagent®RT oral LD50 1620 mg/kg, dermal LD50; >1000 mg/kg. Mouse: (phenol) 270 mg/kg LD50 oral. DOT Dermal

Toxicity Test, 49CFR 173.137, Class 8, Packing Group II Corrosive.



Consensus Exposure Guidelines



OSHA permissible exposure limit (PEL): (phenol) 5 ppm (19 mg / m3) as an 8-hour time-weighted average. Skin notation.



NIOSH recommended exposure limit (REL): (phenol) 5 ppm (19 mg / m3) as a time-weighted average for up to a 10-hour

workday and a 40-hour workweek. Short-term exposure limit (STEL): 15.6 ppm (60 mg / m3) for periods not to exceed 15

minutes. Skin Notation.



ACGIH TLV: (phenol) 5 ppm (19 mg / m3) as a time-weighted average for up to a 8-hour workday and a 40-hour workweek.

Skin Notation.





12. ECOLOGICAL INFORMATION



ECOTOXICOLOGICAL INFORMATION: Short-term toxic effects are expected to be limited to the immediate area of

environmental release, and will be most pronounced in microorganisms. Environmental fate: Expected to rapidly

decompose in the environment. Environmental Movement and Partitioning: Short-term movement could be due to high

water solubility. Movement expected to be limited by relatively rapid environmental detoxification. Expected to partition

strongly into aqueous environmental components.





13. DISPOSAL CONSIDERATIONS



EPA WASTE NUMBER (RCRA HAZARD CLASS) U188. All waste disposal activities are subject to federal, state and

local laws and regulations. Handle as hazardous waste by keeping in sealed containers until final disposal in a hazardous

waste incinerator.





14. TRANSPORTATION INFORMATION



Department of Transportation Hazard Classification (DOT) 49CFR172.101; CORROSIVE LIQUID, N.O.S.; UN1760

Department of Transportation Labeling requirements 49CFR172.101; CLASS 8 PACKING GROUP II, PACKING

INSTRUCTIONS 808

Department of Transportation Packaging requirements 49CFR173.202; EXCEPTIONS: 49CFR173.154

Shipping designation: (TRI Reagent: guanidine thiocyanate-phenol solution).





15. REGULATORY INFORMATION



OSHA: Classified as A HAZARDOUS CHEMICAL@ under US OSHA HAZCOM REGULATION.

TSCA: Some constituents of this product included in US EPA Toxic Substance Control Act (40 CFR part 710).

SARA SECTION 302 Threshold Planning Quantity: 500/10,000 lbs.

SARA SECTION 304 Reportable quantity: 1000 lbs.

CERCLA SECTION 103 Reportable quantity: 1000 lbs.

SUBJECT TO SARA SECTION 313 Annual toxic chemical release reporting.

EUROPEAN UNION:

                        5 PPM PHENOL IN AIR, 19MG/M3 PHENOL WITH A SKIN WARNING

MAC (GERMANY):

EINECS#:                2036327

RISK PHASE:             WARNING!

R24/25                  TOXIC IN CONTACT WITH SKIN AND IF SWALLOWED.

R34                     CAUSES BURNS

SAFETY PHRASE:

S28                     AFTER CONTACT WITH SKIN, WASH IMMEDIATELY WITH PLENTY OF DETERGENT

AND WATER.

S45                     IN CASE OF ACCIDENT, OR IF YOU FEEL UNWELL, SEEK MEDICAL ADVICE.

SAFETY SYMBOL:          TOXIC





16. OTHER INFORMATION                  Reviewed by      BW, MJ

                                       Revision date    01-11-08

This information is believed to be accurate and represents the information currently available to us. However, we make no

warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no

liability resulting from its use. Users should make their own investigations to determine the suitability of the information for

their particular purposes.



Additional Information is available at (http://www.osha.gov/STLC/healthguidelines/phenol/recognition.html)



Abbreviations:

TLV-STEL                   Threshold Limit Values (Short-Term Exposure Limit)

TLV-TWA                    Threshold Limit Values (Time-Weighted Average)

OSHA PEL-TWA               OSHA Permissible Exposure Level (Time-Weighted Average)