MATERIAL SAFETY DATA SHEET
EMERGENCY TELEPHONE NO. 1-800-632-5227
OTHER INFORMATION CALLS 1-978-927-5054
FAX: 1-978-921-1350
Polymerase
New England Biolabs
32 Tozer Road info@neb.com
INTERNET e-mail:
#M0267
Beverly MA 01915
9/05
REVISION DATE:
SECTION 1鈥揅HEMICAL INFORMATION
Product Name: Taq DNA Polymerase SARA 313
1. Taq DNA Polymerase Cas. #9012-90-2 NO
2. Glycerol 50% Cas. #56-81-5 NO
3. Tris-HCI < 1% Cas. #1185-53-1 NO
4. KCl < 1% Cas. #7447-40-7 NO
5. Dithiothreitol < 1% Cas. #3483-12-3 NO
6. EDTA < 1% Cas. #60-00-4 NO
7. Tween-20 < 1% Cas. #9005-64-5 NO
6. NP-40 < 1% Cas. #127087-87-0NO
SECTION 2鈥揅OMPOSITION/ INFORMATION ON INGREDIENT
EC No: 200-289-5
MF: C3H803
CHEMICAL NAME: GLYCEROL
CAS No.: 56-81-5
SYNONYMS: CITIFLOUR AF 2 * GLYCERIN * GLYCERIN, ANHYDROUS * GLYCERINE * GLYCERIN MIST (ACGIH, OSHA) * GLYCERIN,
SYNTHETIC * GLYCERITOL GLYCYL ALCOHOL * CLYZERIN, WASSERFREI (GERMAN) * GROCOLENE * OSMOGLYN * 1,2,3-PROPANE-
TRIOL * STAR * SYNTHETIC GLYCERIN * TECHNICAL GLYCERINE * TRIHYDROXYPROPANE * 1,2,3-TRIHYDROXYPROPANE.
SECTION 3鈥揌AZARDOUS IDENTIFICATION
EMERGENCY OVERVIEW
Caution: Avoid contact and inhalation.
HMIS RATING
HEALTH: 2
FLAMMABILITY: 0
REACTIVITY: 0
NFPA RATING
HEALTH: 2
FLAMMABILITY: 0
REACTIVITY: 0
For additional information on toxicity, please refer to Section 11.
SECTION 4 鈥揊IRST AID MEASURES
ORAL EXPOSURE: If swallowed, wash out mouth with water provided person is conscious. Call a physician.
INHALATION EXPOSURE: If inhaled, remove to fresh air.If breathing becomes difficult, call a physician.
DERMAL EXPOSURE: In case of contact, immediately wash skin with soap and copious amounts of water. Remove clothing and call a
physician.
EYE EXPOSURE: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flush-
ing by separating the eyelids with fingers. Call a physician.
SECTION 5鈥揊IRE FIGHTING MEASURES
Extinguishing Media: Water Spray, Carbon Dioxide, Dry Chemical powder or appropriate foam. Suitable: Use extinguishing media appropri-
ate to surrounding fire conditions.
Unusual Fire and Explosions Hazard (s): Emits toxic toxic fumes under fire conditions.
Special Firefighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
Prevent contact with skin and eyes.
Flash Point: N/A
Autoignition Temp: N/A
Flammability: N/A
SECTION 6 鈥? ACCIDENTAL RELEASE MEASURES
PROCEDURE(S) OF PERSONAL PRECAUTION(S): METHODS FOR CLEANING UP:
Absorb on sand or vermiculite and place in a closed
Exercise appropriate precautions to minimize direct contact with
container for disposal.
skin or eyes and prevent inhalation of vapors
Ventilate area and wash spill site after material pickup is complete.
Wear disposable coveralls and discard them after use.
SECTION 7 鈥? HANDLING AND STORAGE
HANDLING: User Exposure: Avoid contact with eyes, skin, and clothing.
Avoid inhalation. Avoid prolonged or repeated exposure.
STORAGE: Suitable: Keep tightly closed. Store at -20掳C
SECTION 8鈥揈XPOSURE CONTROLS /PPE
Engineering Controls: Safety shower and eye bath. Mechanical exhaust required.
Personal Protective Equipment: General Hygiene Measures:
Wash thoroughly after handling.
Respiratory
Wash contaminated clothing before use.
NIOSH/MSHA-approved respirator.
AVOID INHALATION
Hand:
Compatible chemical-resistant gloves. Keep Tightly Closed
Store in a cool dry place.
Eye:
Freeze. Store at 鈥?20掳C
Compatible safety goggles.
SECTION 9鈥? PHYSICAL AND CHEMICAL PROPERTIES
Physical Properties: Explosion Limits in Air:
Vapor Density: 3.1 G/L
Lower: 0.9%
Melting Point: 20掳 C
PH: 5.5鈥?8.0
Specific Gravity: 1.262
Boiling Point: 182掳 C
Solubility: Water -Z26130
Flash Point: > 392 F, > 200掳 C
Vapor Pressure: < 1 MMHG @ 20掳C
SECTION 10 鈥? STABILITY AND REACTIVITY
Stability: Stable Hazardous Decomposition Products:
Materials to Avoid: Carbon Monoxide, Carbon Dioxide
Strong oxidizing agents, strong bases.
Hazardous Polymerization: Will not occur.
PROTECT FROM HEAT AND MOISTURE
SECTION 11鈥揟OXICOLOGICAL INFORMATION
Route of Exposure:
Multiple Routes
Skin Contact Eye Contact:
May be harmful by inhalation, ingestion, or skin absorption
May cause skin irritation May cause eye irritation
Materials may be irritating to mucous membranes and upper respiratory tract. Chronic Effects: Target Organs, Kidney
Signs and Symptoms of Exposure: Prolonged exposure can cause: RTECS #:MA8050000
Nausea, headache and vomiting
To the best of our knowledge, the properties have not yet been thoroughly investigated.
IRRITATION DATA:
SKN-RBT 5OO MG/24H MLD 85JCAE -, 207, 1986
EYE-RBT 126 MG MLD BIOFX* 9-4/970
EYE-RBT 5OO MG/24H MLD 85JCAE -, 207, 1986
TOXICITY DATA:
ORL-RAT LD5O: 12600 MG/KG FEPRA7 4, 142, 1945
IHL-RAT LC5O: > 570 MG/M3/1H BIOFX* 9-4/970
IPR-RAT LD5O: 4420 MG/KG RCOCB8 56, 125,1987
NIIRDN 6, 215, 1982
SCU-RAT LD5O: 100 MG/KG
IVN-RAT LD5O: 5566 MG/KG ARZNAD 26,1581,1976
Page 2
ORL-MUS LD50: 4090 MG/KG FRZKAP (6), 56, 1977
ARZNAD 28,1579,1978
IPR-MUS LD50: 8700 MG/KG
NIIRDN 6, 215, 1982
SCU-MUS LD5O: 91 MG/KG
IVN-MUS LD5O: 4250 MG/KG JAPMA8 39, 583, 1950
DMDJAP 31, 276,1959
ORL-RBT LD5O: 27 GM/KG
BIOFX* 9-4/970
SKN-RBT LD5O: >10 GM/KG
NIIRDN 6, 215, 1982
IVN-RBT LD5O: 53 GM/KG
JIHTAB 23, 259, 1941
ORL-GPG LD5O: 7750 MG/KG
TARGET ORGAN DATA:
Behavioral (headache) Paternal effects (testes, epididymis, sperm duct)
Gastrointestinal (nausea or vomiting) Effects on fertility (male ferility index)
Kidney, ureter, bladder (changes in tubules) Effects on fertility (post-implantation mortality)
Kidney, ureter, bladder (changes in urine composition) Only selected registry of toxic effects of chemical substance
(RTECS) data is presented here. See actual entry in RTECS
Paternal effects (spermatogenesis)
SECTION 12鈥揈COLOGICAL INFORMATION
Data not yet available
SECTION 13鈥揇ISPOSAL CONSIDERATIONS
Contact a licensed professional waste disposal service to dispose of this material.
Observe all federal state and local environmental regulations.
Dissolve or mix the material with a combustIble solvent and burn in a chemical incinerator equipped with an afterburner and scrubber.
SECTION 14鈥? TRANSPORT INFORMATION
DOT
IATA
Proper Shipping Name: None
Non-Hazardous for Air Transport: Non-hazardous for air transport.
Non-hazardous for transport
This substance is considered to be non-hazardous for transport
SECTION 15鈥? REGULATORY INFORMATION
US CLASSIFICATION AND LABEL TEXT
US Statements: Caution: Avoid contact and inhalation.
UNITED STATES REGULATORY INFORMATION
SARA LISTED: No
CANADA REGULATORY INFORMATION
WHMIS Classification: This product has been classified in
accordance with the hazard criteria of the CPR, and the
MSDS contains all the information required by the CPR.
DSL: No
NDSL: No
SECTION 16鈥揜EGULATORY INFORMATION
DISCLAIMER: For R&D use only. Not for drug, household or other uses.
WARRANTY: The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our knowledge and is applicable to the product with regard to appropriate safety
precautions. It does not represent any guarantee of the properties of the product. Sigma-Aldrich Inc., shall not be held liable for any damage
resulting from handling or from contact with the above product. See reverse side of invoice or packing slip for additional terms and conditions
of sale.
SECTION 17鈥? OTHER INFORMATION
The above information is believed to be correct but New England Biolabs shall not be held liable
does not purport to be all inclusive and shall be used for any damage resulting from handling or
only as a guide. from contact with the above product.
Page 3
|