MATERIAL SAFETY DATA SHEET
Telephone: (978)927-5054
Toll free: (800)632-5227
Kit
Fax: (978)921-1350
New England Biolabs
e-mail: info@neb.com
#E0542
240 Country Road
Ipswich, MA 01938
Msds Revision Date: 1/07
SECTION 1鈥揅HEMICAL INFORMATION
Product Name: Quick Blunting Kit SARA 313 NO
SECTION 2鈥揅OMPOSITION/INFORMATION ON INGREDIENT
NAME OF CHEMICALS:
1. Glycerol 50 % Cas. #56-81-5 NO
SECTION 3鈥揌AZARDOUS IDENTIFICATION
EMERGENCY OVERVIEW
Caution: Avoid contact and inhalation.
HMIS RATING
HEALTH: 2
FLAMMABILITY: 0
REACTIVITY: 0
NFPA RATING
HEALTH: 2
FLAMMABILITY: 0
REACTIVITY: 0
For additional information on toxicity, please refer to Section 11.
SECTION 4鈥揊IRST AID MEASURES
ORAL EXPOSURE: If swallowed, wash out mouth with water provided person is conscious. Call a physician.
INHALATION EXPOSURE: If inhaled, remove to fresh air.If breathing becomes difficult, call a physician.
DERMAL EXPOSURE: In case of contact, immediately wash skin with soap and copious amounts of water. Remove clothing and call a
physician.
EYE EXPOSURE: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate
flushing by separating the eyelids with fingers. Call a physician.
SECTION 5鈥揊IRE FIGHTING MEASURES
Extinguishing Media: Suitable: Water spray.
Carbon Dioxide, dry chemical powder or appropriate foam.
Special Firefighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
Specific Hazard(s): Emits toxic fumes under fire conditions.
Flash point: N/A
Flammability: N/A
Autoignition Temp: N/A
Page 1
MATERIAL SAFETY DATA SHEET
NEW ENGLAND
EMERGENCY TELEPHONE NO. 1-800-632-5227
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KIT
OTHER INFORMATION CALLS 1-978-927-5054
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New England Biolabs
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SECTION 1 鈥揚RODUCT
Product Name: Quick Ligation Kit
SECTION 2 鈥揅HEMICAL INFORMATION
1. Glycerol 50% Cas. #56-81-5
2. PEG 15% Cas. #None
3. Tris-HCl < 1% Cas. #77-86-1
4. Potassium Chloride < 1% Cas. #7447-40-7
5. EDTA < 1% Cas. #60-00-4
6. Dithiothreitol < 1% Cas. #3483-12-3
7. BSA < 1% Cas. #None
8. Magnesium Chloride < 1% Cas. #7786-30-3
9. ATP < 1% Cas. #42170-89-4
SECTION 3鈥揅OMPOSITION/ INFORMATION ON INGREDIENT
CHEMICAL NAME: GLYCEROL
EC No.: 200-289-5
CAS No.: 56-81-5 MF: C3H803
SYNONYMS: CITIFLOUR AF 2 * GLYCERIN * GLYCERIN, ANHYDROUS * GLYCERINE * GLYCERIN MIST (ACGIH, OSHA) * GLYCERIN,
SYNTHETIC * GLYCERITOL GLYCYL ALCOHOL * CLYZERIN, WASSERFREI (GERMAN) * GROCOLENE * OSMOGLYN * 1,2,3-
PROPANETRIOL * STAR * SYNTHETIC GLYCERIN * TECHNICAL GLYCERINE * TRIHYDROXYPROPANE * 1,2,3-
TRIHYDROXYPROPANE.
SECTION 4鈥揌AZARDOUS IDENTIFICATION
LABEL PRECAUTIONARY STATEMENTS:
Target Organ (S)
CAUTION
Kidney
Avoid contact by inhalation, skin and ingestion. Hygroscopic
SECTION 5 鈥揊IRST AID MEASURES
ORAL EXPOSURE: If swallowed, wash out mouth with water provided person is conscious. Call a physician.
INHALATION EXPOSURE: If inhaled, remove to fresh air. If breathing is difficult, call a physician.
DERMAL EXPOSURE: In case of contact, immediately wash skin with soap and copious amounts of water.
Remove clothing and call a physician.
EYE EXPOSURE: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes.
Assure adequate flushing by separating the eyelids with fingers. Call a physician.
SECTION 6鈥揊IRE FIGHTING MEASURES
Extinguishing Media: Unusual Fire and Explosions Hazard (s):
Emits toxic toxic fumes under fire conditions.
Water Spray
Carbon Dioxide, Dry Chemical powder or appropriate foam
Prevent contact with skin and eyes.
Special Firefighting Procedures: Wear self contained breathing
apparatus and protective clothing to prevent contact with skin and eyes.
SECTION 7 鈥? ACCIDENTAL RELEASE MEASURES
METHODS FOR CLEANING UP:
PROCEDURE(S) OF PERSONAL PRECAUTION(S):
Absorb on sand or vermiculite and place in a closed
Wear self-contained breathing apparatus, chemical safety goggles,
container for disposal.
rubber boots, and chemical resistant gloves.
Ventilate area and wash spill site after material pickup is complete.
Wear disposable coveralls and discard them after use.
SECTION 8 鈥? HANDLING AND STORAGE
Refer to Section 8
SECTION 9鈥揈XPOSURE CONTROLS /PPE
Engineering Controls: Safety shower and eye bath. Mechanical exhaust required.
Personal Protective Equipment: General Hygiene Measures:
Respiratory Wash thoroughly after handling.
NIOSH/MSHA-approved respirator. Wash contaminated clothing before use.
Hand: AVOID INHALATION
Compatible chemical-resistant gloves.
KEEP TIGHTLY CLOSED
Eye: STORE IN A COOL DRY PLACE
Compatible safety goggles.
FREEZE.
STORE AT 鈥?20掳C
SECTION 10鈥? PHYSICAL AND CHEMICAL PROPERTIES
Physical Properties: Explosion Limits in Air: Vapor Density: 3.1 G/L
Melting Point: 20掳 C Lower: 0.9% PH: 5.5鈥?8.0
Specific Gravity: 1.262
Boiling Point: 182掳 C
Solubility: Water -Z26130
Flash Point: > 392 F, > 200掳 C
Vapor Pressure: < 1 MMHG @ 20掳C
SECTION 11 鈥? STABILITY AND REACTIVITY
Stability: Stable Hazardous Decomposition Products:
Carbon Monoxide, Carbon Dioxide
Materials to Avoid:
Hazardous Decomposition Products: Will not occur.
Strong oxidizing agents, strong bases.
PROTECT FROM HEAT
SECTION 12鈥揟OXICOLOGICAL INFORMATION
Route of Exposure:
Eye Contact: Multiple Routes
Skin Contact
May cause eye irritation
May cause skin irritation May be harmful by inhalation, ingestion, or skin absorption
Materials may be irritating to mucous membranes and upper respiratory tract.
RTECS #:MA8050000 Chronic Effects: Target Organs, Kidney
GLYCEROL
To the best of our knowledge, the properties have not yet been thoroughly investigated.
IRRITATION DATA:
SKN-RBT 5OO MG/24H MLD 85JCAE -, 207, 1986
EYE-RBT 126 MG MLD BIOFX* 9-4/970
EYE-RBT 5OO MG/24H MLD 85JCAE -, 207, 1986
TOXICITY DATA:
ORL-RAT LD5O: 12600 MG/KG FEPRA7 4, 142, 1945
IHL-RAT LC5O: > 570 MG/M3/1H BIOFX* 9-4/970
IPR-RAT LD5O: 4420 MG/KG RCOCB8 56, 125,1987
NIIRDN 6, 215, 1982
SCU-RAT LD5O: 100 MG/KG
IVN-RAT LD5O: 5566 MG/KG ARZNAD 26,1581,1976
ORL-MUS LD50: 4090 MG/KG FRZKAP (6), 56, 1977
ARZNAD 28,1579,1978
IPR-MUS LD50: 8700 MG/KG
NIIRDN 6, 215, 1982
SCU-MUS LD5O: 91 MG/KG
IVN-MUS LD5O: 4250 JAPMA8 39, 583, 1950
MG/KG
ORL-RBT LD5O: 27 DMDJAP 31, 276,1959
GM/KG
BIOFX* 9-4/970
SKN-RBT LD5O: >10 GM/KG
IVN-RBT LD5O: 53 GM/KG NIIRDN 6, 215, 1982
JIHTAB 23, 259, 1941
ORL-GPG LD5O: 7750 MG/KG
Page 2 (Glycerol)
TARGET ORGAN DATA:
Behavioral (headache) Paternal effects (testes, epididymis, sperm duct)
Gastrointestinal (nausea or vomiting) Effects on fertility (male ferility index)
Kidney, ureter, bladder (changes in tubules) Effects on fertility (post-implantation mortality)
Kidney, ureter, bladder (changes in urine composition) Only selected registry of toxic effects of chemical substance
Paternal effects (spermatogenesis) (RTECS) data is presented here. See actual entry in RTECS
SECTION 13鈥揈COLOGICAL INFORMATION
Data not yet available
SECTION 14鈥揇ISPOSAL CONSIDERATIONS
Contact a licensed professional waste disposal service to dispose of this material.
Observe all federal state and local environmental regulations.
Dissolve or mix the material with a combustable solvent and burn in a chemical incinerator equipped with an afterburner and scrubber.
SECTION 15鈥? TRANSPORT INFORMATION
Contact New England Biolabs, Inc. Company for Transportation Information.
SECTION 16鈥? REGULATORY INFORMATION
Reviews, standards and Regulations:
OEL=MAK
ACGIH TLV-TWA 10 MG/M3 DTLVS* TLV/BEI, 1999
EPA FIFRA 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTER FEREAC 54, 7740, 1989.
MSHA STANDARD: NUISANCE PARTICULATES (MIST)
OEL -Australia: TWA 10 MG/M3, JAN 1993
DTLWS* 3, 20, 1973
OEL -Belgium: TWA 10 MG/M3, JAN 1993
OSHA PEL (GEN INDU): 8H TWA 15 MG/M3, TOTAL DUST
OEL -Finland: TWA 20 MG/M3, JAN 1999
CFRGBR 29, 1910.1000, 1994
OEL -France: VME 10 MG/M3, JAN 1999
OSHA PEL (GEN INDU): 8H TWA 5 MG/M3, RESPIRABLE FRACTION
OEL -The Netherlands: MAC-TGG 10 MG/M3, Jan 1999
CFRGBR 29, 1910.1000, 1994
OEL -United Kindgom: TWA 10 MG/M3, MIST Sept 2000
OSHA PEL (CONSTRUC): 8H TWA 15 MG/M3, TOTAL DUST
OEL in Argentina, Bulgaria, Colombia, Jordan, Korea Check ACC
CFRGBR 29, 1926.55, 1994
NOHS 1974: HZD 35085; NIS 358; TNF 86657; NOS 198;
OSHA PEL (CONSTRUC): 8H TWA 5 MG/M3, RESPIRABLE FRACTION
TNE 1085329
CFRGBR 29, 1926.55, 1994
NOHS 1983: HZD 35085; NIS 310; TNF 67054; NOS 215;
OSHA PEL (SHIPYARD): 8H TWA 15 MG/M3, TOTAL DUST
TNE 2135546; TFE 1346631
CFRGBR 29, 1915.1000, 1993
EPA TSCA SECTION 8 (B) Chemical Inventory
OSHA PEL (SHIPYARD): 8H TWA 5 MG/M3, RESPIRABLE FRACTION
EPA TSCA SECTION 8 (D) Unpublished Health/Safety Studies
CFRGBR 29, 1915.1000, 1993
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE,
Jan. 2001
CHEMICAL: PEG
SECTION 1 - PRODUCT
PRODUCT NAME: Polyethylene glycol
SECTION 2鈥揅OMPOSITION/ INFORMATION ON INGREDIENT
CAS No.: NONE
SECTION 3鈥揌AZARDOUS IDENTIFICATION
DATA NOT AVAILABLE
SECTION 4 鈥揊IRST AID MEASURES
ORAL EXPOSURE: If swallowed, wash out mouth with water provided person is conscious. Call a physician.
INHALATION EXPOSURE: If inhaled, remove to fresh air. If breathing is difficult, give oxygen and call a physician.
DERMAL EXPOSURE: In case of contact, immediately wash skin with soap and copious amounts of water.
EYE EXPOSURE: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes.
SECTION 5鈥揊IRE FIGHTING MEASURES
Extinguishing Media: Water spray. Unusual Fire and Explosions Hazard (s):
Carbon Dioxide, dry chemical powder or appropriate foam. Emits toxic fumes under fire conditions.
Special Firefighting Procedures: Wear self contained breathing Fire Fighting Protective Procedures:
apparatus and protective clothing to prevent contact with skin and eyes. Wear self-contained breathing apparatus and protective
clothing to prevent contact with skin and eyes.
SECTION 6 - ACCIDENTAL RELEASE MEASURES
EVACUATE AREA
PROCEDURE(S) OF PERSONAL PRECAUTION(S): METHODS FOR CLEANING UP:
Wear self-contained breathing apparatus, chemical safety Absorb on sand or Vermiculite and place in closed containers disposal.
goggles, rubber boots, and heavy rubber gloves.
Ventilate area and wash spill site after material pickup is complete.
SECTION 7 - HANDLING AND STORAGE
Refer To Section 8
SECTION 8- EXPOSURE CONTROLS /PPE
Engineering Controls: Safety shower and eye bath. Mechanical exhaust required.
Personal Protective Equipment: AVOID ALL CONTACT WITH EYE, SKIN, CLOTHING.
Respiratory General Hygiene Measures:
NIOSH/MSHA-approved respirator.
Wash thoroughly after handling.
Hand:
Wash contaminated clothing before use.
Compatible chemical-resistant gloves.
Avoid prolonged or repeated exposure.
Eye: Keep Tightly closed
Chemical safety goggles.
Refrigerate
SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES
Appearance and Odor: Liquid
SECTION 10 - STABILITY AND REACTIVITY
Stability: Stable Hazardous Decomposition Products:
Carbon monoxide, Carbon dioxide.
Materials to Avoid
Strong oxidizing agents Hazardous Polymerization: Will not occur
SECTION 11- TOXICOLOGICAL INFORMATION
Route of Exposure:
Multiple Routes
Eye Contact:
Skin Contact
May cause skin irritation May cause eye irritation May be harmful by inhalation, ingestion, or skin absorption
May be irritating to mucous membranes and upper respiratory tract.
To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated.
SECTION 12- ECOLOGICAL INFORMATION
Data not yet available
SECTION 13- DISPOSAL CONSIDERATIONS
Contact a licensed professional waste disposal service to dispose of this material.
Dissolve or mix the material with a combustable solvent and burn in a chemical incinerator equipped with an afterburner and scrubber.
Observe all federal state and local environmental regulations.
SECTION 14- TRANSPORT INFORMATION
Contact New England Biolab, Inc. Company for Transportation Information.
SECTION 15- REGULATORY INFORMATION
European Information:
CAUTION: SUBSTANCE NOT YET FULLY TESTED
S 24/25
AVOID ALL CONTACT WITH SKIN AND EYES.
SECTION 16鈥? OTHER INFORMATION
New England Biolabs shall not be held liable
The above information is believed to be correct but
for any damage resulting from handling or
does not purport to be all inclusive and shall be
from contact with the above product.
used only as a guide.
SECTION 6鈥揂CCIDENTAL RELEASE MEASURES
PROCEDURE(S) OF PERSONAL PRECAUTION(S): Wear self-contained breathing apparatus, rubber boots and heavy rubber
gloves and Chemical safety googles.
METHODS FOR CLEANING UP: Cover with dry lime, sand, or soda ash. Place in covered containers using non-sparking tools and
transport outdoors. Ventilate area and wash spill site after material pickup is complete.
SECTION 7鈥揌ANDLING AND STORAGE
Handling: User Exposure: Avoid inhalation. Avoid contact with eyes, skin and clothing. Avoid prolonged or repeated exposure.
Storage: Suitable: Keep tightly closed.
SECTION 8鈥揈XPOSURE CONTROLS/PPE
Engineering Controls: Safety shower and eye bath. Mechanical exhaust required.
Personal Protective Equipment: Goverment approved respirator.
Hand: Compatible chemical-resistant gloves.
Eye: Chemical safety goggles.
General Hygiene Measures: Wash hands thoroughly after handling. Wash contaminated clothing before use.
SECTION 9鈥揚HYSICAL AND CHEMICAL PROPERTIES
Physical Properties:
Melting Point: 20掳C
Boiling Point: 182掳C
Flash Point: 320掳F, 160掳C Method: closed up
Autoignition Temp: 370掳C
Explosion Limits in Air:
Lower: 0.9%
Specific Gravity: 1.262
Solubility: Water -Z26130
Vapor Pressure: < 1 MMHG @ 20掳C
Vapor Density: 3.1 G/L
PH: 5.5鈥?8.0
SECTION 10鈥揝TABILITY AND REACTIVITY
Stability: Stable
Materials to Avoid: Strong oxidizing agents.
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide, sulfur dioxides.
Hazardous Polymerization: Hazardous Polymerization: Will not occur
SECTION 11鈥揟OXICOLOGICAL INFORMATION
Route of exposure:
Skin contact: May cause skin irritaiton.
Multiple Routes: May be harmful by inhalation, ingestion, or skin absorption.
Eye contact: May cause eye irritation.
Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract.
Signs and Symptoms of Exposure: Nausea, headache and vomiting. To the best of our knowledge, the properties
have not been thoroughly investigated.
IRRITATION DATA:
SKN-RBT 5OO MG/24H MLD 85JCAE -, 207, 1986
EYE-RBT 126 MG MLD BIOFX* 9-4/970
EYE-RBT 5OO MG/24H MLD 85JCAE -, 207, 1986
Page 2
TOXICITY DATA:
ORL-RAT LD5O: 12600 MG/KG FEPRA7 4, 142, 1945
IHL-RAT LC5O: > 570 MG/M3/1H BIOFX* 9-4/970
IPR-RAT LD5O: 4420 MG/KG RCOCB8 56, 125,1987
SCU-RAT LD5O: 100 MG/KG NIIRDN 6, 215, 1982
IVN-RAT LD5O: 5566 MG/KG ARZNAD 26,1581,1976
ORL-MUS LD50: 4090 MG/KG FRZKAP 6), 56, 1977
IPR-MUS LD50: 8700 MG/KG ARZNAD 28,1579,1978
SCU-MUS LD5O: 91 MG/KG NIIRDN 6, 215, 1982
IVN-MUS LD5O: 4250 MG/KG JAPMA8 39, 583, 1950
ORL-RBT LD5O: 27 GM/KG DMDJAP 31, 276,1959
SKN-RBT LD5O: >10 GM/KG BIOFX* 9-4/970
IVN-RBT LD5O: 53 GM/KG NIIRDN 6, 215, 1982
ORL-GPG LD5O: 7750 MG/KG JIHTAB 23, 259, 1941
TARGET ORGAN DATA:
Behavioral (headache)
Gastrointestinal (nausea or vomiting)
Kidney, ureter, bladder (changes in tubules)
Kidney, ureter, bladder (changes in urine comp)
Paternal effects (spermatogenesis)
Paternal effects (testes, epididymis, sperm duct)
Effects on fertility (male ferility index)
Effects on fertility (post-implantation mortality
Only selected registry of toxic effects of chemical substance
(RTECS) data is presented here. See actual entry in RTECS
SECTION 12鈥揈COLOGICAL INFORMATION
No Data Available.
SECTION 13鈥揇ISPOSAL CONSIDERATIONS
Contact a licensed professional waste disposal service to dispose of this material. This combustible material may be burned in a chemi-
cal incinerator equipped with an afterburner and scrubber. Observe all federal, state and local environmental regulations.
SECTION 14鈥揟RANSPORT INFORMATION
DOT
Proper Shipping Name: None
Non-Hazardous for Air Transport: non-hazardous for air transport.
IATA
Non-Hazardous for Air Transport: non-hazardous for air transport.
SECTION 15鈥揜EGULATORY INFORMATION
US Classification and Label Text
US Statements: Avoid contact and inhalation.
United States Regulatory Information:
Sara Listed: No
Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR
and the MSDS contains all the information required by the CPR.
DSL: Yes
NDSL: No
SECTION 16鈥揙THER INFORMATION
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide.
New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product.
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