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MSDS Material Safety Data Sheet
CAS

56-81-5
7647-14-5
77-86-1
60-00-4

File Name: neb_com---msdsm0240.asp
                                                       MATERIAL SAFETY DATA SHEET
NEW ENGLAND
EMERGENCY TELEPHONE NO. 1-800-632-5227

BioLabs 庐

Glycosylase
OTHER INFORMATION CALLS 1-978-927-5054
Inc.
FAX 1-978-921-1350
New England Biolabs
#M0240
info@neb.com
INTERNET e-mail:
32 Tozer Road
Beverly MA 01915 JUNE, 2003
DATE



SECTION 1 鈥揅HEMICAL INFORMATION

Product Name: Fpg


SECTION 2 鈥揅HEMICAL INFORMATION

1. Glycerol 50% Cas. #56-81-5
2. Sodium Chloride < 1% Cas. #7647-14-5
3. Tris-HCl < 1% Cas. #77-86-1
4. EDTA < 1% Cas. #60-00-4
5. BSA < 1% Cas. #None


SECTION 3鈥揅OMPOSITION/ INFORMATION ON INGREDIENT

CHEMICAL NAME: GLYCEROL

EC No.: 200-289-5
CAS No.: 56-81-5 MF: C3H803
SYNONYMS: CITIFLOUR AF 2 * GLYCERIN * GLYCERIN, ANHYDROUS * GLYCERINE * GLYCERIN MIST (ACGIH, OSHA) * GLYCERIN,
SYNTHETIC * GLYCERITOL GLYCYL ALCOHOL * CLYZERIN, WASSERFREI (GERMAN) * GROCOLENE * OSMOGLYN * 1,2,3-
PROPANETRIOL * STAR * SYNTHETIC GLYCERIN * TECHNICAL GLYCERINE * TRIHYDROXYPROPANE * 1,2,3-
TRIHYDROXYPROPANE.

SECTION 4鈥揌AZARDOUS IDENTIFICATION
LABEL PRECAUTIONARY STATEMENTS:
Target Organ (S)
CAUTION
Kidney
Avoid contact by inhalation, skin and ingestion. Hygroscopic


SECTION 5 鈥揊IRST AID MEASURES

ORAL EXPOSURE: If swallowed, wash out mouth with water provided person is conscious. Call a physician.

INHALATION EXPOSURE: If inhaled, remove to fresh air. If breathing is difficult, call a physician.

DERMAL EXPOSURE: In case of contact, immediately wash skin with soap and copious amounts of water.
Remove clothing and call a physician.

EYE EXPOSURE: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes.
Assure adequate flushing by separating the eyelids with fingers. Call a physician.

SECTION 6鈥揊IRE FIGHTING MEASURES
Extinguishing Media: Unusual Fire and Explosions Hazard (s):
Emits toxic toxic fumes under fire conditions.
Water Spray
Carbon Dioxide, Dry Chemical powder or appropriate foam
Prevent contact with skin and eyes.
Special Firefighting Procedures: Wear self contained breathing
apparatus and protective clothing to prevent contact with skin and eyes.
SECTION 7 鈥? ACCIDENTAL RELEASE MEASURES
METHODS FOR CLEANING UP:
PROCEDURE(S) OF PERSONAL PRECAUTION(S):
Absorb on sand or vermiculite and place in a closed
Wear self-contained breathing apparatus, chemical safety goggles,
container for disposal.
rubber boots, and chemical resistant gloves.
Ventilate area and wash spill site after material pickup is complete.
Wear disposable coveralls and discard them after use.

SECTION 8 鈥? HANDLING AND STORAGE
Refer to Section 8

SECTION 9鈥揈XPOSURE CONTROLS /PPE
Engineering Controls: Safety shower and eye bath. Mechanical exhaust required.
Personal Protective Equipment: General Hygiene Measures:
Respiratory Wash thoroughly after handling.
NIOSH/MSHA-approved respirator. Wash contaminated clothing before use.
Hand: AVOID INHALATION
Compatible chemical-resistant gloves.
KEEP TIGHTLY CLOSED
Eye: STORE IN A COOL DRY PLACE
Compatible safety goggles.
FREEZE.
STORE AT 鈥?20掳C

SECTION 10鈥? PHYSICAL AND CHEMICAL PROPERTIES
Physical Properties: Explosion Limits in Air: Vapor Density: 3.1 G/L
Melting Point: 20掳 C Lower: 0.9% PH: 5.5鈥?8.0
Specific Gravity: 1.262
Boiling Point: 182掳 C
Solubility: Water -Z26130
Flash Point: > 392 F, > 200掳 C
Vapor Pressure: < 1 MMHG @ 20掳C

SECTION 11 鈥? STABILITY AND REACTIVITY
Stability: Stable Hazardous Decomposition Products:
Carbon Monoxide, Carbon Dioxide
Materials to Avoid:
Hazardous Decomposition Products: Will not occur.
Strong oxidizing agents, strong bases.

PROTECT FROM HEAT

SECTION 12鈥揟OXICOLOGICAL INFORMATION
Route of Exposure:
Eye Contact: Multiple Routes
Skin Contact
May cause eye irritation
May cause skin irritation May be harmful by inhalation, ingestion, or skin absorption
Materials may be irritating to mucous membranes and upper respiratory tract.
RTECS #:MA8050000 Chronic Effects: Target Organs, Kidney
GLYCEROL
To the best of our knowledge, the properties have not yet been thoroughly investigated.

IRRITATION DATA:
SKN-RBT 5OO MG/24H MLD 85JCAE -, 207, 1986
EYE-RBT 126 MG MLD BIOFX* 9-4/970
85JCAE -, 207, 1986
EYE-RBT 5OO MG/24H MLD

TOXICITY DATA:
ORL-RAT LD5O: 12600 MG/KG FEPRA7 4, 142, 1945
IHL-RAT LC5O: > 570 MG/M3/1H BIOFX* 9-4/970
IPR-RAT LD5O: 4420 MG/KG RCOCB8 56, 125,1987
NIIRDN 6, 215, 1982
SCU-RAT LD5O: 100 MG/KG
IVN-RAT LD5O: 5566 MG/KG ARZNAD 26,1581,1976
ORL-MUS LD50: 4090 MG/KG FRZKAP (6), 56, 1977
ARZNAD 28,1579,1978
IPR-MUS LD50: 8700 MG/KG


Page 2 (Glycerol)
NIIRDN 6, 215, 1982
SCU-MUS LD5O: 91 MG/KG
JAPMA8 39, 583, 1950
IVN-MUS LD5O: 4250 MG/KG
DMDJAP 31, 276,1959
ORL-RBT LD5O: 27 GM/KG
BIOFX* 9-4/970
SKN-RBT LD5O: >10 GM/KG
IVN-RBT LD5O: 53 GM/KG NIIRDN 6, 215, 1982
JIHTAB 23, 259, 1941
ORL-GPG LD5O: 7750 MG/KG


TARGET ORGAN DATA:
Behavioral (headache) Paternal effects (testes, epididymis, sperm duct)
Gastrointestinal (nausea or vomiting) Effects on fertility (male ferility index)
Kidney, ureter, bladder (changes in tubules) Effects on fertility (post-implantation mortality)
Kidney, ureter, bladder (changes in urine composition) Only selected registry of toxic effects of chemical substance
Paternal effects (spermatogenesis) (RTECS) data is presented here. See actual entry in RTECS

SECTION 13鈥揈COLOGICAL INFORMATION
Data not yet available


SECTION 14鈥揇ISPOSAL CONSIDERATIONS

Contact a licensed professional waste disposal service to dispose of this material.
Observe all federal state and local environmental regulations.
Dissolve or mix the material with a combustable solvent and burn in a chemical incinerator equipped with an afterburner and scrubber.



SECTION 15鈥? TRANSPORT INFORMATION

This product does not contain hazardous compounds in
quantities that require special handling precautions.

SECTION 16鈥? REGULATORY INFORMATION

Reviews, standards and Regulations:
OEL=MAK
ACGIH TLV-TWA 10 MG/M3 DTLVS* TLV/BEI, 1999
EPA FIFRA 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTER FEREAC 54, 7740, 1989.
MSHA STANDARD: NUISANCE PARTICULATES (MIST)
OEL -Australia: TWA 10 MG/M3, JAN 1993
DTLWS* 3, 20, 1973
OEL -Belgium: TWA 10 MG/M3, JAN 1993
OSHA PEL (GEN INDU): 8H TWA 15 MG/M3, TOTAL DUST
OEL -Finland: TWA 20 MG/M3, JAN 1999
CFRGBR 29, 1910.1000, 1994
OEL -France: VME 10 MG/M3, JAN 1999
OSHA PEL (GEN INDU): 8H TWA 5 MG/M3, RESPIRABLE FRACTION
OEL -The Netherlands: MAC-TGG 10 MG/M3, Jan 1999
CFRGBR 29, 1910.1000, 1994
OEL -United Kindgom: TWA 10 MG/M3, MIST Sept 2000
OSHA PEL (CONSTRUC): 8H TWA 15 MG/M3, TOTAL DUST
OEL in Argentina, Bulgaria, Colombia, Jordan, Korea Check ACC
CFRGBR 29, 1926.55, 1994
NOHS 1974: HZD 35085; NIS 358; TNF 86657; NOS 198;
OSHA PEL (CONSTRUC): 8H TWA 5 MG/M3, RESPIRABLE FRACTION
TNE 1085329
CFRGBR 29, 1926.55, 1994
NOHS 1983: HZD 35085; NIS 310; TNF 67054; NOS 215;
OSHA PEL (SHIPYARD): 8H TWA 15 MG/M3, TOTAL DUST
TNE 2135546; TFE 1346631
CFRGBR 29, 1915.1000, 1993
EPA TSCA SECTION 8 (B) Chemical Inventory
OSHA PEL (SHIPYARD): 8H TWA 5 MG/M3, RESPIRABLE FRACTION
EPA TSCA SECTION 8 (D) Unpublished Health/Safety Studies
CFRGBR 29, 1915.1000, 1993
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE,
Jan. 2001

SECTION 17鈥? OTHER INFORMATION
New England Biolabs shall not be held liable
The above information is believed to be correct but
for any damage resulting from handling or
does not purport to be allinclusive and shall be
from contact with the above product.
used only as a guide.

Page 3 (Glycerol)

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