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MSDS Material Safety Data Sheet
CAS

56-81-5
7647-14-5
77-86-1
60-00-4

File Name: neb_com---msdsm0247.asp
                                                       MATERIAL SAFETY DATA SHEET
Telephone: (978)927-5054
Toll free: (800)632-5227
Nuclease
Fax: (978)921-1350
#M0247
New England BioLabs e-mail: info@neb.com
240 County Road
Ipswich, MA 01938 REVISION DATE: 3/07



SECTION 1 鈥揅HEMICAL INFORMATION

Product Name: Micrococcal Nuclease
1. Glycerol 50% Cas. #56-81-5
2. Sodium Chloride > 1% Cas. #7647-14-5
3. Tris-HCl < 1% Cas. #77-86-1
4. EDTA < 1% Cas. #60-00-4




SECTION 2鈥揅OMPOSITION/ INFORMATION ON INGREDIENT

CHEMICAL NAME: GLYCEROL
CAS No.: 56-81-5 SARA 313: No
Formula: C3H803
SYNONYMS: CITIFLOUR AF 2 * GLYCERIN * GLYCERIN, ANHYDROUS * GLYCERINE * GLYCERIN MIST (ACGIH, OSHA) * GLYCERIN,
SYNTHETIC * GLYCERITOL GLYCYL ALCOHOL * CLYZERIN, WASSERFREI (GERMAN) * GROCOLENE * OSMOGLYN * 1,2,3-PROPANE-
TRIOL * STAR * SYNTHETIC GLYCERIN * TECHNICAL GLYCERINE * TRIHYDROXYPROPANE * 1,2,3-TRIHYDROXYPROPANE.



RTECS Number: MA8050000

SECTION 3鈥揌AZARDOUS IDENTIFICATION
HMIS Rating NFPA Rating
Emergency Overview:
Health: 1
Health: 1*
Target Organ (S)
CAUTION
Flammability: 0
Kidney Flammability: 0
Avoid contact by inhalation,
Reactivity: 1
Hygroscopic Reactivity: 1
skin and ingestion.

* additional chronic hazards present

SECTION 4 鈥揊IRST AID MEASURES
ORAL EXPOSURE: If swallowed, wash out mouth with water provided person is conscious. Call a physician.

INHALATION EXPOSURE: If inhaled, remove to fresh air. If breathing is difficult, call a physician.
DERMAL EXPOSURE: In case of contact, immediately wash skin with soap and copious amounts of water.
Remove clothing and call a physician.
EYE EXPOSURE: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes.
Assure adequate flushing by separating the eyelids with fingers. Call a physician.


SECTION 5鈥揊IRE FIGHTING MEASURES
Extinguishing Media: Water Spray Unusual Fire and Explosions Hazard (s):
Carbon Dioxide, Dry Chemical powder or appropriate foam Emits toxic toxic fumes under fire conditions.
Special Firefighting Procedures: Wear self contained breathing Flammability: N/A
apparatus and protective clothing to prevent contact with skin and eyes. Prevent contact with skin and eyes.
SECTION 6 鈥? ACCIDENTAL RELEASE MEASURES
PROCEDURE(S) OF PERSONAL PRECAUTION(S): METHODS FOR CLEANING UP:
Absorb on sand or vermiculite and place in a closed
Exercise appropriate precautions to minimize direct contact with
container for disposal.
skin or eyes and prevent inhalation of vapors
Ventilate area and wash spill site after material pickup is complete.
Wear disposable coveralls and discard them after use.


SECTION 7 鈥? HANDLING AND STORAGE
Handling Special Requirements
Storage
User Exposure: Avoid inhalation.
Hygroscopic
Suitable: Keep tightly closed.
Avoid contact with eyes, skin and clothing.
Avoid prolonged or repeated exposure.


SECTION 8鈥揈XPOSURE CONTROLS /PPE
General Hygiene Measures:
Engineering Controls: Safety shower and eye bath. Mechanical exhaust required.
Wash thoroughly after handling.
Personal Protective Equipment: Wash contaminated clothing before use.
Eye:
Respiratory AVOID INHALATION
Compatible safety goggles.
NIOSH/MSHA-approved respirator.
Keep Tightly Closed
Hand: Store in a cool dry place.
Compatible chemical-resistant gloves.

Exposure Limits, RTECS
Country Source Type Value
USA USA ACGIH ACGIH TWA TWA 10 MG/M3 10 MG/M3
Remarks: inhalable particulate
USA USA MSHA Standard MSHA
Remarks: Nuisance Particulates (mist.) Nuisance
USA USA OSHA OSHA. PEL PEL 8H TWA 15 MG/M3, Total Dust 8H
New Zealand OEL OEL
Remarks: check ACGIH TLV check ACGIH TLV

Exposure Limits
Type
Source Value
Country
Poland NDS 10 mg/m3
Poland NDSCh 鈥?
Poland NDSP
Remarks: (OELS are valid for aerosols) aerozole

SECTION 9鈥? PHYSICAL AND CHEMICAL PROPERTIES
Physical Properties: Explosion Limits in Air:
Vapor Density: 3.1 G/L
Lower: 0.9%
Melting Point: 20掳 C
PH: 5.5鈥?8.0
Specific Gravity: 1.262
Boiling Point: 182掳 C
Solubility: Water -Z26130
Flash Point: 320 F, 160掳 C Method: closed up
Autoignition Temp: 370掳 C Vapor Pressure: < 1 MMHG @ 20掳C

SECTION 10 鈥? STABILITY AND REACTIVITY
Stability: Stable Hazardous Decomposition Products:
Carbon Monoxide, Carbon Dioxide
Materials to Avoid:
Strong oxidizing agents, strong bases.
Hazardous Polymerization:
PROTECT FROM HEAT AND MOISTURE Hazardous Polymerization: Will not occur.

SECTION 11鈥揟OXICOLOGICAL INFORMATION
Route of Exposure:
Multiple Routes
Eye Contact:
Skin Contact
May be harmful by inhalation, ingestion, or skin absorption
May cause eye irritation
May cause skin irritation
Materials may be irritating to mucous membranes and upper respiratory tract. Chronic Effects: Target Organs, Kidney
Signs and Symptoms of Exposure: Prolonged exposure can RTECS #:MA8050000
cause: Nausea, headache and vomiting
To the best of our knowledge, the properties have not yet been thoroughly investigated.
Page 2
IRRITATION DATA:
SKN-RBT 5OO MG/24H MLD 85JCAE -, 207, 1986
EYE-RBT 126 MG MLD BIOFX* 9-4/970
EYE-RBT 5OO MG/24H MLD 85JCAE -, 207, 1986

TOXICITY DATA:
ORL-RAT LD5O: 12600 MG/KG FEPRA7 4, 142, 1945
IHL-RAT LC5O: > 570 MG/M3/1H BIOFX* 9-4/970
IPR-RAT LD5O: 4420 MG/KG RCOCB8 56, 125,1987
NIIRDN 6, 215, 1982
SCU-RAT LD5O: 100 MG/KG
IVN-RAT LD5O: 5566 MG/KG ARZNAD 26,1581,1976
ORL-MUS LD50: 4090 MG/KG FRZKAP (6), 56, 1977
ARZNAD 28,1579,1978
IPR-MUS LD50: 8700 MG/KG
NIIRDN 6, 215, 1982
SCU-MUS LD5O: 91 MG/KG
IVN-MUS LD5O: 4250 MG/KG JAPMA8 39, 583, 1950
DMDJAP 31, 276,1959
ORL-RBT LD5O: 27 GM/KG
BIOFX* 9-4/970
SKN-RBT LD5O: >10 GM/KG
NIIRDN 6, 215, 1982
IVN-RBT LD5O: 53 GM/KG
JIHTAB 23, 259, 1941
ORL-GPG LD5O: 7750 MG/KG
TARGET ORGAN DATA:
Behavioral (headache) Paternal effects (testes, epididymis, sperm duct)
Gastrointestinal (nausea or vomiting) Effects on fertility (male ferility index)
Kidney, ureter, bladder (changes in tubules) Effects on fertility (post-implantation mortality)
Kidney, ureter, bladder (changes in urine composition) Only selected registry of toxic effects of chemical substance
(RTECS) data is presented here. See actual entry in RTECS
Paternal effects (spermatogenesis)

SECTION 12鈥揈COLOGICAL INFORMATION
Data not yet available

SECTION 13鈥揇ISPOSAL CONSIDERATIONS
Contact a licensed professional waste disposal service to dispose of this material.
Observe all federal state and local environmental regulations.
Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber.


SECTION 14鈥? TRANSPORT INFORMATION
DOT
IATA
Proper Shipping Name: None
Non-Hazardous for Air Transport: Non-hazardous for air transport.
Non-hazardous for transport
This substance is considered to be non-hazardous for transport

SECTION 15鈥? REGULATORY INFORMATION
US Classification and Label Text:
EU Additional Classification
US Statements: Caution: Avoid contact and inhalation.
S: 23 24/25 Target Organ(s): Kidneys
Safety Statements: Do not breathe vapor.
Avoid contact with skin and eyes.:
United States Regulatory Information:
Sara Listed: No
Canada Regulatory Information
TSCA Inventory Item: Yes Yes
WHMIS Classification: This product has been classification in accordance with the
hazard criteria of the CPR and the MSDS contains all the
information required by the CPR
DSL: Yes
NDSL: No

SECTION 16鈥? OTHER INFORMATION
DISCLAMER
For R&D use only. Not for drug, household or other uses.

New England Biolabs shall not be held liable
The above information is believed to be correct but
for any damage resulting from handling or
does not purport to be all inclusive and shall be used
from contact with the above product.
only as a guide.
Page 3
MATERIAL SAFETY DATA SHEET
Telephone: (978)927-5054
Toll free: (800)632-5227
Buffer
Fax: (978)921-1350
New England Biolabs
email: info@neb.com
240 County Road
#B0247
Ipswich, MA 01938
REVISION DATE: 3/07


SECTION 1鈥揚RODUCT

Product Name: Micrococcal Nuclase Reaction Buffer

SECTION 2鈥揅HEMICAL INFORMATION

1. Tris-HCl < 1% Cas. #77-86-1
2. Calcium Chloride < 1% Cas. #10035-05-8




SECTION 3鈥揌AZARDOUS INFORMATION

Many NEB prouucts are classiofifed mixtures which cantain hazardous ingredients in concentrations
(less than 1%)> The quantity of hazardous chemical component does not exceed the permissible limit
or "Threshold Limit Value".

MSDS' are offered by NRB since The Hazards Communication Standard set by OSHA does not
exempt these mixtures from disclosure.



SECTION 4鈥揙THER INFORMATION


The above information is believed to be correct New England Biolabs shall not be held liable for any
but does not purport to be all inclusive and shall damage resulting from handling or from contact with
be used only as a guide. the above product.

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