JVL MATERIAL SAFETY DATA SHEET
MSDS FOR REPLICATION-DEFECTIVE
ADENOVIRAL VECTORS (Biosafety Level 2)
Cultures of replication defective adenoviral vectors are non-infectious and are not
hazardous materials as defined by OSHA 1919.1200. However, these materials are
produced in cells where there is the possibility of recombination to form wild type
virus. As such, they should be handled as potentially infectious material.
Description:
Adenoviral vectors consist of recombinant transgene sequences (e.g., marker or human genes) inserted into
an E1A-deleted adenovirus. The E1A deletion renders the vector replication defective and dependent upon
E1A and other viral sequences present in human 293 cells. Adenoviral cultures are normally provided as
purified viral particles in phosphate buffered saline at a concentration of up to 5x1012 particles/ml. The viral
stock consists of particles containing the vector genome (infectious particles) and a variable number of
empty viral capsids in PBS. Other trace components present include, but are not limited to, inorganic salts,
vitamins and other nutrients, and human cellular proteins, carbohydrates, amino acids, and fats. The
material is normally shipped and stored frozen.
SECTION I
Hazardous Ingredients
None
SECTION II
Physical Data
Liquid or frozen particle suspensions
SECTION III
Health Hazards
Adenoviral cultures are not known to cause any diseases in humans or animals
SECTION IV
Fire and Explosion
None
SECTION V
Reactivity
Not chemically reactive. Will enter mammalian cells and interact or react with cellular components
University of North Carolina 7017 Neurosciences Hospital
Gene Therapy Center Chapel Hill, NC 27599-7592
Joint Vector Laboratories Phone: 919-962-1221
JVL MATERIAL SAFETY DATA SHEET
SECTION VI
Method of Disposal
Spill: Contain spill and decontaminate the area using a disinfectant such as chlorine bleach (10%
f.c.), Wescodyne, or detergent-based disinfectant.
Waste Disposal: Dispose of viral stock by autoclaving at 121oC for 30-45 minutes
Dispose of infected liquid cultures by decontamination with chlorine bleach (10%
f.c.) for 10 minutes and then dispose of in sink.
Dispose of infected animal carcasses or tissues by incineration
Follow all Federal, State, and Local regulations.
SECTION VII
Special Protective Information
Handle as biohazardous material under Biosafety Level 2 containment
SECTION VIII
Special Precautions or Comments
The Gene Therapy Center recommends that all Adenoviral vectors and cultures be handled by
qualified microbiologists using appropriate safety procedures and precautions. Detailed discussions
of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice
(Fleming et al., ASM Press, Washington D.C., 1995), and in the U.S. Government Publication,
Biosafety in Microbiological and Biomedical Laboratories (CDC, 1999). This and other
publications are available at the Centers for Disease Control Office of Health and Safety鈥檚 website at
http://www.cdc.gov/od/ohs/biosfty/bmb14/bmb14toc.htm
Information on the classification of human etiologic agents on the basis of hazard can be found as
Appendix B in the NIH Guidelines for Research Involving Recombinant DAN Molecules at
http://www.grants.nih.gov/grants/policy/recombinentdnaguidelines.htm
The above information is accurate to the best of our knowledge. All materials and mixtures may
present unknown hazards and should be used with caution. The user should exercise independent
judgment as to the hazards based on all sources of information available. The Gene Therapy Center
shall not be held liable for any damage resulting from the handling or use of the above product
University of North Carolina 7017 Neurosciences Hospital
Gene Therapy Center Chapel Hill, NC 27599-7592
Joint Vector Laboratories Phone: 919-962-1221
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