Page 1/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 01/25/2008 Reviewed on 01/25/2008
1 Identification of substance:
路 Product name: Lyse/Fix Buffer 5X
路 Catalog number: 558049
路 Manufacturer/Supplier:
BD Biosciences, Pharmingen
10975 Torreyana Road
San Diego, CA 92121-1106
Tel. (877)232-8995
路 Information department: Technical Services
路 Emergency information:
In case of a chemical emergency, spill, fire, exposure, or accident please call ChemTrec at (800)424-9300 or 911
2 Composition/Data on components:
路 Chemical characterization
路 Description: Mixture of the substances listed below with nonhazardous additions.
路 Dangerous components:
50-00-0 formaldehyde Carc. Cat. 3; T, C; R 23/24/25-34-40-43 37.0%
111-46-6 diethylene glycol Xn; R 22 15.0%
3 Hazards identification
路 Hazard description:
T Toxic
路 Information pertaining to particular dangers for man and environment
R 23/24/25 Toxic by inhalation, in contact with skin and if swallowed.
R 34 Causes burns.
R 37 Irritating to respiratory system.
R 40 Limited evidence of a carcinogenic effect.
R 43 May cause sensitization by skin contact.
路 Classification system
The classification was made according to the latest editions of international substances lists, and expanded upon
from company and literature data.
路 NFPA ratings (scale 0-4)
Health = 3
1 Fire = 1
3 0 Reactivity = 0
4 First aid measures
路 General information
Immediately remove contaminated clothing.
Remove breathing apparatus only after contaminated clothing have been completely removed.
(Contd. on page 2)
USA
DR
Page 2/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 01/25/2008 Reviewed on 01/25/2008
Product name: Lyse/Fix Buffer 5X
(Contd. of page 1)
In case of irregular breathing or respiratory arrest provide artificial respiration.
路 After inhalation
Supply fresh air or oxygen; and immediately call for a doctor.
In case of unconsciousness place patient on side position for transportation.
路 After skin contact Immediately wash with water and soap and rinse thoroughly.
路 After eye contact Rinse opened eye for 15 minutes under running water. Then consult a doctor.
路 After swallowing
If swallowed, rinse mouth with water (only if the person is conscious)
Drink copious amounts of water and provide fresh air. Immediately call a doctor.
Seek immediate medical advice.
路 Information for doctor Show the product label or this MSDS.
5 Fire fighting measures
路 Suitable extinguishing agents Use fire fighting measures that suit the environment.
路 Protective equipment: No special measures required.
6 Accidental release measures
路 Person-related safety precautions: Not required.
路 Measures for environmental protection: Wipe up with damp sponge or mop.
路 Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders).
Use neutralizing agent.
Dispose contaminated material as waste according to item 13.
Ensure adequate ventilation.
路 Additional information:
See Section 7 for information on safe handling.
See Section 8 for information on personal protection equipment.
See Section 13 for disposal information.
7 Handling and storage
路 Handling
路 Information for safe handling:
Ensure good ventilation/exhaustion at the workplace.
Open and handle receptacle with care.
Prevent formation of aerosols.
路 Information about protection against explosions and fires: Keep respiratory protective device available.
路 Storage
路 Requirements to be met by storerooms and receptacles: No special requirements.
路 Information about storage in one common storage facility: Store away from oxidizing agents.
路 Further information about storage conditions: Store in cool, dry conditions in well sealed containers.
USA
(Contd. on page 3)
DR
Page 3/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 01/25/2008 Reviewed on 01/25/2008
Product name: Lyse/Fix Buffer 5X
(Contd. of page 2)
8 Exposure controls and personal protection
路 Additional information about design of technical systems: No further data; see item 7.
路 Components with limit values that require monitoring at the workplace:
50-00-0 formaldehyde
PEL Short-term value: 2 ppm
Long-term value: 0.75 ppm
see 29 CFR 1910.1048
REL Short-term value: C 0.1* ppm
Long-term value: 0.016 ppm
*15-min
TLV Short-term value: C 0.37 mg/m鲁, C 0.3 ppm
SEN
111-46-6 diethylene glycol
WEEL 10 mg/m鲁
路 Additional information: The lists that were valid during the creation were used as basis.
路 Personal Protective Equipment
路 General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
Immediately remove all soiled and contaminated clothing.
Wash hands before breaks and at the end of work.
Store protective clothing separately.
Avoid contact with the eyes and skin.
路 Breathing equipment:
In case of brief exposure, use a chemical fume hood or a NIOSH/MSHA-approved respirator.
路 Protection of hands:
Chemical resistant gloves (i.e. nitrile, or equivalent).
路 Eye protection:
Safety glasses
Tightly sealed goggles.
路 Body protection: Protective work clothing (lab coat).
9 Physical and chemical properties:
路 General Information
Liquid
Form:
Clear
Color:
Characteristic
Odor:
路 Change in condition
Melting point/Melting range: Not determined
Boiling point/Boiling range: -21掳C (-6掳F)
146掳C (295掳F)
路 Flash point:
(Contd. on page 4)
USA
DR
Page 4/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 01/25/2008 Reviewed on 01/25/2008
Product name: Lyse/Fix Buffer 5X
(Contd. of page 3)
225掳C (437掳F)
路 Ignition temperature:
Product is not self igniting.
路 Auto igniting:
Product does not present an explosion hazard.
路 Danger of explosion:
路 Explosion limits:
0.7 Vol %
Lower:
73.0 Vol %
Upper:
路 Vapor pressure at 20掳C (68掳F): 1.7 hPa (1 mm Hg)
Not determined
路 Density:
路 Solubility in / Miscibility with
Soluble
Water:
7.1
路 pH-value at 20掳C (68掳F):
路 Solvent content:
52.0 %
Organic solvents:
10 Stability and reactivity
路 Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
路 Materials to be avoided: Incompatible material: strong oxidizers.
路 Dangerous reactions No dangerous reactions known
路 Dangerous products of decomposition: No dangerous decomposition products known.
11 Toxicological information
路 Acute toxicity:
路 LD/LC50 values that are relevant for classification:
50-00-0 formaldehyde
Oral LD50 42 mg/kg (rat)
路 Primary irritant effect:
路 on the skin: Caustic effect on skin and mucous membranes.
路 on the eye: Strong caustic effect.
路 Sensitization: No sensitizing effects known.
路 Additional toxicological information:
The product shows the following dangers according to internally approved calculation methods for preparations:
Toxic
Corrosive (causes skin burns)
Irritant
Swallowing will lead to a strong caustic effect on mouth and throat and to the danger of perforation of esophagus
and stomach.
USA
(Contd. on page 5)
DR
Page 5/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 01/25/2008 Reviewed on 01/25/2008
Product name: Lyse/Fix Buffer 5X
(Contd. of page 4)
12 Ecological information:
路 Ecotoxical effects:
路 Other information:
The ecological effects have not been thoroughly investigated, but currently none have been identified.
路 General notes: Water hazard class 2 (Self-assessment): hazardous for water.
13 Disposal considerations
路 Product:
路 Recommendation
Must not be disposed of with solid waste.
Dispose of material in accordance with federal (40 CFR 261), state and local requirements.
This product is not considered a RCRA hazardous waste.
路 Uncleaned packagings:
路 Recommendation: Disposal must be made according to state and federal regulations.
路 Recommended cleansing agent: Water, if necessary with cleansing agents.
14 Transport information
路 DOT regulations:
8
路 Hazard class:
路 Identification number: UN2209
III
路 Packing group:
路 Proper shipping name: FORMALDEHYDE SOLUTION, mixture
8
路 Label
路 Land transport ADR/RID (cross-border)
8 (C9) Corrosive substances
路 ADR/RID class:
路 Danger code (Kemler): 80
2209
路 UN-Number:
III
路 Packaging group:
路 Description of goods: 2209 FORMALDEHYDE SOLUTION, mixture
路 Maritime transport IMDG:
8
路 IMDG Class:
(Contd. on page 6)
USA
DR
Page 6/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 01/25/2008 Reviewed on 01/25/2008
Product name: Lyse/Fix Buffer 5X
(Contd. of page 5)
2209
路 UN Number:
8
路 Label
III
路 Packaging group:
F-A,S-B
路 EMS Number:
No
路 Marine pollutant:
路 Proper shipping name: FORMALDEHYDE SOLUTION, mixture
路 Air transport ICAO-TI and IATA-DGR:
8
路 ICAO/IATA Class:
2209
路 UN/ID Number:
8
路 Label
III
路 Packaging group:
路 Proper shipping name: FORMALDEHYDE SOLUTION, mixture
15 Regulations
路 SARA Section 355 (extremely hazardous substances)
50-00-0 formaldehyde
路 SARA Section 313 (specific toxic chemical listings)
50-00-0 formaldehyde
路 TSCA (Toxic Substances Control Act)
50-00-0 formaldehyde
111-46-6 diethylene glycol
路 California Proposition 65 - Chemicals known to cause cancer
Formaldehyde is toxic, allergenic, and is known to the state of California to cause cancer. Note: Formaldehyde
poses a cancer threat through inhalation of the gas or vapor.
50-00-0 formaldehyde
路 Chemicals known to cause reproductive toxicity for females:
None of the ingredients is listed.
路 Chemicals known to cause reproductive toxicity for males:
None of the ingredients is listed.
路 Chemicals known to cause developmental toxicity:
None of the ingredients is listed.
路 Carcinogenicity categories
路 IARC (International Agency for Research on Cancer)
50-00-0 formaldehyde 2A
路 NTP (National Toxicology Program)
50-00-0 formaldehyde R
(Contd. on page 7)
USA
DR
Page 7/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 01/25/2008 Reviewed on 01/25/2008
Product name: Lyse/Fix Buffer 5X
(Contd. of page 6)
路 TLV (Threshold Limit Value established by ACGIH)
None of the ingredients is listed.
路 Product related hazard information:
The product has been classified and marked in accordance with regulations on hazardous materials.
路 Hazard symbols:
T Toxic
路 Hazard-determining components of labeling:
formaldehyde
路 Risk phrases:
23/24/25 Toxic by inhalation, in contact with skin and if swallowed.
34 Causes burns.
37 Irritating to respiratory system.
40 Limited evidence of a carcinogenic effect.
43 May cause sensitization by skin contact.
路 Safety phrases:
3 Keep in a cool place.
4 Keep away from living quarters.
26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
36/37/39 Wear suitable protective clothing, gloves and eye/face protection.
45 In case of accident or if you feel unwell, seek medical advice immediately.
60 This material and its container must be disposed of as hazardous waste.
路 National regulations
路 Technical instructions (air):
Class Share in %
I 37.0
NK 15.0
路 Water hazard class: Water hazard class 2 (Self-assessment): hazardous for water.
16 Other information:
To the best of our knowledge, the information contained herein is accurate. However, neither Becton,Dickinson
and Company or any of its subsidiaries assumes any liabilities whatsoever for the accuracy or completeness of
the information contained herein. Final determination of suitability of any material is the sole responsibility of
the user. All materials may present unknown hazards and should be used with caution. Although certain hazards
are described herein, we cannot guarantee that these are the only hazards that exist.
路 Department issuing MSDS: Safety and Environment Department
路 Contact: Technical Service Representative
USA
DR
|