MATERIAL SAFETY DATA SHEET
Telephone: (978)927-5054
Competent
Toll free: (800)632-5227
Cells
Fax: (978)921-1350
New England Biolabs
#C2987
e-mail: info@neb.com
240 Country Road
Revision Date: 10/07
Ipswich, MA 01938
SECTION 1鈥揅HEMICAL INFORMATION
Product Name: NEB 5-alpha Competent E. coli (High Efficiency)
SECTION 2鈥揅OMPOSITION/INFORMATION ON INGREDIENT
Substance Name:
The hazards indentified with this product are those associated with the following component (s):
Dimethyl Sulfoxide 0鈥?10% Cas. #67-68-5 SARA 313: No
Glycerol 0鈥?10% Cas. #56-81-5 SARA 313: No
SECTION 3鈥揌AZARDOUS IDENTIFICATION
HMIS Rating NFPA Rating
Emergency Overview: The hazards identified with this product
Health: 0* Health: 0
are those associated with the following component(s):
Flammability: 0 Flammability: 0
Dimethyl Sulfoxide and Glycerol.
Reactivity: 0 Reactivity: 0
For additional information on toxicity, please refer to Sec. 11.
SECTION 4鈥揊IRST AID MEASURES
Oral Exposure: If swallowed, wash out mouth with water provided person is conscious. Call a physician.
Inhalation Exposure: If inhaled, remove to fresh air. If not breathing give artificial respiration.
If breathing is difficult, give oxygen. Call a physician.
Dermal Exposure: In case of contact, immediately wash skin with soap and copious amounts of water.
Eye Exposure: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes.
Assure adequate flushing by separating the eyelids with fingers. Call a physician.
SECTION 5鈥揊IRE FIGHTING MEASURES
Extinguishing Media: Suitable: Water spray. Carbon Dioxide, dry chemical powder or appropriate foam.
Special Firefighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with
skin and eyes.
Specific Hazard(s): Combustible liquid. Emits toxic fumes under fire conditions.
Flash point: 188.6掳F, 87掳C Method closed cup.
Flammability: N/A
Autoignition Temp: 301掳C
Explosin Limits: Lower 3.5%, Upper: 42%
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SECTION 6鈥揂CCIDENTAL RELEASE MEASURES
Procedure(s) Of Personal Precaution(s): Wear self-contained breathing apparatus, rubber boots and heavy rubber gloves
and Chemical safety googles.
Methods For Cleaning Up: Cover with dry lime, sand, or soda ash. Place in covered containers using non-sparking tools
and transport outdoors. Ventilate area and wash spill site after material pickup is complete.
Environmental Precaution(s): Avoid contaminating water supply. Avoid contaminating sewers and waterways with this
material.
Procedure To Be Followed in Case Of Leak or Spill: Evacuate area.
SECTION 7鈥揌ANDLING AND STORAGE
Handling: Avoid prolonged or repeated exposure.
User Exposure: Avoid Inhalation. Avoid contact with DMSO solutions containing toxic materials or material with unknown
toxicological properties. Dimethyl sulfoxide is readily absorbed through skin and may carry such materials into the body. Avoid
prolonged or repeated exposure.
Storage: Keep tightly closed, away from sparks and open flames. Store in a cool dry place.
Special Requirements: Store under inert gas. Hygroscropic.
SECTION 8鈥揈XPOSURE CONTROLS/PPE
Engineering Controls: Safety shower and eye bath. Mechanical exhaust required.
Personal Protective Equipment: Goverment approved respirator.
Hand: Compatible chemical-resistant gloves.
Eye: Chemical safety goggles.
Skin Specific: Chemical resistant
General Hygiene Measures: Wash hands thoroughly after handling. Wash contaminated clothing before use.
SECTION 9鈥揚HYSICAL AND CHEMICAL PROPERTIES
Appearance: Physical State: Clear Liquid Color: Colorless
Property Value At temperature or Pressure
Molecular Weight: 78.13 AMU
pH: N/A
BP/BP Range: 89掳C
MP/MP Range: 18.4掳C
Freezing Point: N/A
Vapor Pressure: 0.42 mmHg 20掳C
Vapor Density: 2.7 g/l
Saturated Vapor: N/A
SG/Density: .1 g/cm3
Bulk Density: N/A
Odor Threshold: N/A
Volatile %: N/A
Voc Content: N/A
Water Content: N/A
Solvent Content: N/A
Evaporation Rate: N/A
Viscosity: 0.002 Pas 20掳C
Surface Tension: N/A
Partition Coefficient: Log Kow: 鈥?2.03
Decomposition Temp: > 190掳C
Flash Point: 88.6掳F, 87掳C Method: Closed cup.
Explosion Limits: Lower: 3.5%, Upper: 42%
Flammability: N/A
Autoignition Temp: 01掳C
Refraction Index: 1.479
Optical Rotation: N/A
Miscellaneous Data: N/A
Sollubility in Water: Soluble
N/A = not available Page 2
SECTION 10鈥揝TABILITY AND REACTIVITY
Stability: Stable
Conditions to Avoid: Mosture.
Materials to Avoid: Acid chlorides, Phosphorus halides, strong oxidizing agents, strong acids, strong reducing agents.
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide, Sulfur dioxides.
Hazardous Polymerization: Will not occur.
Hazardous Exothermic Reactions: Hazardous Exothermic Reactions: Methyl sulfoxide (DMSO) undergoes a violent exo-
thermic reaction on mixing with copper wool and trichloroacetic acid. On mixing with potassium permanganate it will flash in-
stantaneously. It reacts violently with: acid halides, cyanuric chloride, silicon tetrachloride, phorphorus trichloride and trioxide,
thionyl chloride, magnesium perchlorate, silver flioride, methyl bromide, iodine pentafluoride, nitrogen periodate, diborane,
sodium hydride and perchloric and periodic acids. When heated above its boiling point methyl sulfoxide degrades giving off
formaldehyde, methyl mercaptan and sulfur dioxide.
SECTION 11- TOXICOLOGICAL INFORMATION
Route of Exposure:
Skin Absorption: May be harmful if absorbed.
Contact: May cause skin irritation.
Eye Contact:May cause eye irritation.
Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract.
Ingestion: May be harmful if swallowed.
Conditions Aggravated By Exposure: Avoid contact with DMSO solutions containing toxic materials or material with
unknown toxicological properties. Dimethyl sulfoxide is readily absorbed through skin and may carry such materials into the
body. Avoid prolonged or repeated exposure.
Target Organ (s) or System (s): Eyes and Skin
Toxicity Data Subcutaneous
Rat
Inhalation
12 gg/kg
Rat
LD50
40,250 ppm
Remarks: Behavorial: Change in motor activity (specific
LC50
assay), Lungs, Thorax, or Respiration:Dyspnea.
Oral
Intravenous
Rat
Rat
3,300 mg/kg
5,360 mg /kg
LD50
LD50
Skin
Remarks: Behavorial: Tremor, Muscle weakness. Lungs,
Rabbit
Thorax or Respiration:Dyspnea.
> 5,000 mg/kg
Oral
LD50
Mouse
Oral
7,920 mg /kg
Rat
LD50
14,500 mg/kg
Skin
LD50
Mouse
Remarks: Sense Organs and Special Senses (Nose, Eye,
50,000 mg /kg
Ear and Taste): Eye: Hemorrhage. Sense Organs and Special
LD50
Senses (Nose, Eye, Ear and Taste): Eye: Conjunctive irritation.
Intrarperitoneal
Skin
Mouse
Rat
2,500 mg /kg
40,000 mg/kg
LD50
LD50
Subcutaneous
Intraperitoneal
Mouse
Rat
14 gm/kg
8,200 mg/kg
LD50
LD50
Remarks: Behavorial: Change in motor activity (specific
assay), Lungs, Thorax, or Respiration: Other changes.
Kidney, Ureter, Bladder: Hematuria.
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Irritation Data Chronic Exposure - Teratogen
Skin Species: Mouse
Rabbit Route of Application: Intraperitineal
4 Hours Dose: 210 mg/kg
Remarks: No irritation effect Exposure Time: (6鈥?12D PREG)
Result: Specific development Annormalities: Central nervous
Eyes
system. Specific development Annormalities: Musculoskel-
Rabbit
etal system.
Remarks: Mild irritation effect
Species: Mouse
Skin
Route of Application: Intraperitineal
Rabbit
Dose: 5,500 mg/kg
10 mg
Exposure Time: (10D PREG)
24H
Result: Effects on Embryo or Fetus: Fetotoxicity (except
Remarks: Open irritation test
death, e.g., stunted fetus). Specific Developmental
Skin
Abnormalities: Musculoskeletal system.
Rabbit
Species: Hamster
500 mg
Route of Application: Oral
24H
Dose: 11 gm/kg
Remarks: Mild irritation effect
Exposure Time: (7D PREG)
Eyes
Result: Effects on Embryo or Fetus: Fetotoxicity (except
Rabbit
death, e.g., stunted fetus). Specific Developmental
100 mg
Abnormalities: Musculoskeletal system.
Eyes
Species: Hamster
Rabbit
Route of Application: Intraperitineal
500 mg
Dose: 5,500 mg/kg
24H
Exposure Time: (8D PREG)
Remarks: Mild irritation effect
Result: Specific development Annormalities: Central nervous
Chronic Exposure - Carcinogen system. Specific development Annormalities: Musculoskele-
tal system. Specific development Annormalities: Craniofacial
Species: Rat
(including nose and tongue).
Route of Application: Oral
Dose: 59 gm/kg Species: Mouse
Exposure Time: 81W Route of Application: Intraperitineal
Frequency: I Dose: 4,400 mg/kg
Result: Tumorigenic:Equivocal tumorigenic agent by RTECS Exposure Time: (8D PREG)
criteria, Skin and Appendages: Other: Tumors. Result: Effects on Embryo or Fetus: Fetus: death. Specific
Developmental Abnormalities: Central nervous system.
Species: Rat
Species: Hamster
Route of Application: Subcutaneous
Dose: 220 gm/kg Route of Application: Intervenous
Exposure Time: 82W Dose: 2,500 mg/kg
Frequency I Exposure Time: (8D PREG)
Result: Tumorigenic:Equivocal tumorigenic agent by RTECS Result: Specific development Annormalities: Central nervous
criteria, Skin and Appendages: Other: Tumors. system. Specific development Annormalities: Musculoskele-
tal system. Specific development Annormalities: Craniofacial
Species: Mouse
(including nose and tongue).
Route of Application: Oral
Dose: 65,340 mg/kg Species: Hamster
Exposure Time: 66W Route of Application: Intervenous
Frequency I Dose: 2,500 mg/kg
Result: Tumorigenic:Equivocal tumorigenic agent by RTECS Exposure Time: (8D PREG)
criteria. Blood:Leukemia Skin and Appendages: Other: Result: Specific development Annormalities:Other
Tumors. developmental abnormalities.
Species: Mouse
Route of Application: Subcutaneous
Dose: 66 gm/kg
Exposure Time: 66W
Frequency I
Result: Tumorigenic:Equivocal tumorigenic agent by RTECS
criteria, Lungs Thorax or Appendages: Other: Tumors. Skin
and Appendages: Other: Tumors.
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Chronic Exposure - Mutagen Species: Mouse
Dose: 16 mg/kg
Species: Human
Route of Application: Oral
Dose: 140 MMOL/L
Exposure Time: (5鈥?9D PREG)
Cell Type: lymphocyte
Result: Effects on Fertility: Pre-implantation mortality (e.g.,
Mutation Test: Other mutation test system.
reduction in number of implants per female; total number
Species: Rat
of implants per corpora lutea). Effects on Embryo or Fetus:
Route: Intraperitoneal
Fetotoxicity (except death, e.g., stunted fetus). Specific
Dose: 25 gm/kg
Developmental Abnormalities: Musculoskeletal system.
Exposue Time: 5D
Species : Mouse
Mutation Test: Cytogenetic analysis.
Dose: 82,50 mg/kg
Species: Mouse
Route of Application: Intraperitoneal
Route: Intraperitoneal
Exposure Time: (10D PREG)
Dose: 75 mmol/kg
Result: Effects on Fertility: Post-implantation mortality (e.g.,
Mutation Test: DNA damage.
dead and/or resorbed implants per total number of implants).
Species: Mouse
Species : Mouse
Route: Intraperitoneal
Dose: 240 gm/kg
Dose: 93 gm/l
Route of Application: Intravenous
Cell Type: lymphocyte
Exposure Time: (1鈥?20D PREG)
Mutation Test: Cytogenetic analysis.
Result: Effects on Fertility: Post-implantation mortality (e.g.,
Species: Mouse dead and/or resorbed implants per total number of implants).
Dose: 1 mol/l
Species: Hamster
Cell Type: lymphocyte
Route of Application: Intervenous
Mutation Test: Mutation in mammalian somatic cells.
Dose: 2,500 mg/kg
Species: Hamster Exposure Time: (8D PREG)
Dose: 19 pph Result: Specific development Annormalities: Central nervous
Cell Type: ovary system, Musculoskeletal system and Craniofacial (including
Mutation Test: Cytogenetic analysis. nose and tongue).
Species: Hamster Species: Hamster
Dose: 1 pph Route of Application: Intervenous
Cell Type: lung Dose: 2,500 mg/kg
Mutation Test: Cytogenetic analysis. Exposure Time: (8D PREG)
Result: Specific development Annormalities:Other
Chronic Exposure - Reproductive Hazard
developmental abnormalities.
Species: Rat
Dose: 56 gm/kg
Route of Application: Intraperitoneal
Exposure Time: (6鈥?12D PREG)
Result: Effects on Fertility: Abortion
Species: Rat
Dose: 6,600 mg/kg
Route of Application: Intraperitoneal
Exposure Time: (7鈥?15D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Species : Rat
Dose: 30,750 mg/kg
Route of Application: Subcutaneous
Exposure Time: (8鈥?10D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Effects on Fertility:Litter size (e.g.; # fetuses per litter;
measured before birth).
Page 5
SECTION 12鈥揈COLOGICAL INFORMATION
Acute Ecotoxicity Tests
Test Type: EC50 Alge
Test Type: LC50 Fish
Species: Lepomis macrochirus (Bluegill)
Species : Onchorhynchus mykiss (Rainbow trout)
Time: 96 h
Time: 96h
Value: > 400,000 mg/1
Value: 35,000 mg/1
Test Type: LC50 Fish
Test Type: EC50 Daphnia
Species: Pimephales promelas (Fathead minnow)
Species : Daphnia pulex
Time: 96 h
Value: 27,500 mg/1
Value: 34,000 mg/1
SECTION 13鈥揇ISPOSAL CONSIDERATIONS
Appropriate Method of Disposal of Substance or Preparation
Contact a licensed professional waste disposal service to dispose of this material. This combustible material
may be burned in a chemical incinerator equipped with an afterburner and scrubber. Observe all federal, state
and local environmental regulations.
SECTION 14鈥揟RANSPORT INFORMATION
IATA
DOT
Non-Hazardous for Air Transport: non-hazardous for air
Proper Shipping Name: Combustible Liquid n.o.s.
transport.
UN # NA1993
Class: Combustible Liquid
Packing Group: Packing Group III
Hazard Label: None
PIH: Not PIH
SECTION 15鈥揜EGULATORY INFORMATION
US Classification and Label Text Canada Regulatory Information WHMIS Classification: This
US Statments: Combustible. Readily absorbed product has been classified in accordance with the hazard
through skin.Target Organ (s): Eyes, Skin. criteria of the CPR and the MSDS contains all the information
required by the CPR.
United States Regulatory Information:
DSL: Yes
Sara Listed: No
TSCA Inventory Item: Yes NDSL: No
SECTION 16鈥揙THER INFORMATION
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a
guide.
New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the
above product.
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