New England Biolabs, Inc.
Tel: 800-632-5227 (orders)
Tel: 800-632-7799 (support)
Fax: 978-921-1350
info@neb.com
www.neb.com
Competent Cells from NEB
Convenient Formats
NEB is pleased to offer several strains of optimized
electrocompetent and chemically competent cells for For your convenience, we offer all of these strains in two
cloning and protein expression. formats; 20 single-use transformation tubes or 6 tubes
containing 200 袖l each. Both formats are supplied with SOC
Cloning Strains outgrowth media and a pUC19 plasmid control. The most
popular cloning strain, NEB 5-alpha is offered at a subcloning
NEB 5-alpha is a high efficiency derivative of DH5留?, the
efficiency for substantial value. NEB 5-alpha, NEB 10-beta and
industry standard cloning strain. It is also offered in a lacI q
NEB Turbo are also available in electrocompetent formats.
version for the cloning of toxic genes. NEB Turbo brings
See www.neb.com for protocols and tips on enhancing
unmatchable speed to your transformations with visible
transformation efficiencies.
colonies after just 6.5 hours. Other cloning strains include
NEB 10-beta, a derivative of DH10B?, an excellent strain for
transforming large plasmids and BACs, as well as dam?/dcm?,
a strain for dam and dcm methylation free plasmid growth.
Advantages
? Extremely high efficiencies
? T1 phage resistance (fhuA2)
? Outgrowth media and control plasmid included
? A variety of convenient formats including single-use
transformation tubes and, on request, 96 well formats
? Quality assurance ? NEB scientists have been
using these strains in house for over 20 years
? Bulk sales capabilities with custom packaging formats
? Free of animal products
Protein Expression Strains
Try our protein expression strains for an extra level of
Strain Selection Chart
confidence. NEB Express is an enhanced BL21 derivative
available with or without the added control of IPTG induced
Characteristics Strain
expression of non-T7 plasmids from lacI q. Several NEB
Fastest Growth ? Colonies Visible After 6.5 Hours NEB Turbo Competent E. coli*
strains feature the lysY gene for exceptional control of
expression. LysY is a variant of T7 lysozyme lacking amidase Versatile Cloning NEB 5-alpha Competent E. coli*
activity making the cells less susceptible to lysis during Cloning of Toxic Genes NEB 5-alpha F卒 I q Competent E. coli
induction, while retaining the ability to inhibit T7 RNA Cloning of Large Plasmids and BACs NEB 10-beta Competent E. coli*
polymerase. Basal expression of the target gene is minimized Growth of Unmethylated Plasmids dam?/dcm? Competent E. coli
without inhibiting IPTG-induced expression. LysY is encoded Most Popular Non-T7 Expression Strain NEB Express Competent E. coli
on a single-copy miniF plasmid that does not require Control of IPTG Induced Expression NEB Express I q Competent E. coli
antibiotic selection for propagation. T7 Express (an enhanced Most Popular T7 Expression Strain T7 Express Competent E. coli
derivative of BL21, (DE3)) is available with or without the
Reduced Basal Expression T7 Express I q Competent E. coli
added control of lacI q, and both versions can be purchased
Tight Control of Protein Expression
with or without the lysY feature. T7 Express lysY/I q provides by Inhibition of T7 RNA Polymerase T7 Express lysY Competent E. coli
the highest level of uninduced control. T7 Express Crystal is a Highest Level of Expression Control T7 Express lysY/I q Competent E. coli
metB strain optimized for crystallographic experiments. For Crystallography Experiments/SeMet Labeling T7 Express Crystal Competent E. coli
*Also available as electrocompetent cells.
DH5留? and DH10B? are trademarks of Invitrogen Corporation.
Competent Cells (1/9/08) (See other side)
Competent Cells from NEB (continued)
Strain Properties Table
NEB T7 T7 T7
NEB NEB 5-alpha NEB dam?/ NEB NEB T7 T7 Express Express Express
F卒 l q Express l q Express l q lysY/l q
Turbo 5-alpha 10-beta dcm? Express Express lysY Crystal
Transformation 1-3 x 10 9 1-3 x 10 9
1-3 x 10 9 1-3 x 10 9 1-3 x 10 6 0.6-1 x 10 9 0.6-1 x 10 9 0.6-1 x 10 9 0.6-1 x 10 9 0.6-1 x 10 9 0.6-1 x 10 9 0.6-1 x 10 9
Efficiency (cfu/袖g)*
K12 K12 K12 K12 K12 B B B B B B
Strain B
T1 Phage 鐃? 鐃? 鐃? 鐃? 鐃? 鐃? 鐃? 鐃? 鐃? 鐃? 鐃?
鐃?
Resistant
Blue/White 鐃? 鐃? 鐃? 鐃? ? ? ? ? ? ? ?
?
Screening
lacl q 鐃? 鐃? 鐃? 鐃? 鐃?
? ? ? ? ? ?
?
lysY 鐃? 鐃? ?
? ? ? ? ? ? ? ?
?
Colonies Visible
?
? ? ? ? ? ?
鐃? ?
?
? ?
after 6.5 hours
Endonuclease I 鐃? 鐃? 鐃?
鐃? 鐃? 鐃? 鐃? 鐃? 鐃?
鐃? 鐃?
鐃?
Deficient
Protease 鐃? 鐃?
鐃? 鐃? 鐃? 鐃?
鐃?
? ? ? ?
?
Deficient
Eukaryotic 鐃? 鐃?
鐃? 鐃? 鐃? 鐃? 鐃?
鐃?
? ? ?
?
DNA Cloning
M13 Phage 鐃? ? ?
鐃? ? ? ? ? ? ?
?
?
Capable (F +)
RecA 鐃? 鐃? 鐃? ? ? ? ? ?
? ? ?
?
Deficient
鐃?
鐃? 鐃?
鐃?
? ? ? ? ? ? 鐃?
?
T7 RNA Polymerase
*Transformation Efficiencies given are for high efficiency chemically competent strains. TE for electrocompetent strains is 1-4 x 1010 cfu/袖g.
Cloning Strains
NEB Turbo Competent E. coli NEB 5-alpha Competent E. coli dam?/dcm? Competent E. coli
Fastest growth ? colonies visible after 6.5 hours Versatile cloning strain Grow plasmids free of dam and dcm
methylation
NEB Turbo Competent E. coli (High Efficiency) NEB 5-alpha Competent E. coli (High Efficiency)
C2984H 20 tubes x 0.05 ml . . . . . . .$223.00 C2987H 20 tubes x 0.05 ml . . . . . . .$166.00 dam?/dcm? Competent E. coli
C2984I 6 tubes x 0.2 ml . . . . . . .$170.00 C2987I 6 tubes x 0.2 ml . . . . . . .$130.00 C2925H 20 tubes x 0.05 ml . . . . . . .$208.00
C2925I 6 tubes x 0.2 ml . . . . . . .$160.00
NEB Turbo Electrocompetent E. coli NEB 5-alpha Competent E. coli
C2986K 6 tubes x 0.1 ml . . . . . . .$208.00 (Subcloning Efficiency)
Companion Product
C2988J 6 tubes x 0.4 ml . . . . . . . . $58.00
SOC Outgrowth Medium
NEB 10-beta Competent E. coli
NEB 5-alpha Electrocompetent E. coli B9020S 100 ml . . . . . . . . $55.00
Clone large plasmids and BACs
C2989K 6 tubes x 0.1 ml . . . . . . .$155.00
NEB 10-beta Competent E. coli (High Efficiency)
C3019H 20 tubes x 0.05 ml . . . . . . .$176.00 NEB 5-alpha F卒 l q Competent E. coli
C3019I 6 tubes x 0.2 ml . . . . . . .$135.00 Clone toxic genes
NEB 10-beta Electrocompetent E. coli NEB 5-alpha F卒 l q Competent E. coli
C3020K 6 tubes x 0.1 ml . . . . . . .$166.00 (High Efficiency)
C2992H 20 tubes x 0.05 ml . . . . . . .$166.00
C2992I 6 tubes x 0.2 ml . . . . . . .$130.00
Protein Expression Strains
NEB Express Competent E. coli T7 Express l q Competent E. coli T7 Express High Efficiency Sampler
Most popular expression strain Reduced basal expression Try each of our four T7 Express strains
NEB Express Competent E. coli T7 Express l q Competent E. coli T7 Express High Efficiency Sampler
(High Efficiency) (High Efficiency) C3009I 8 tubes x 0.2 ml . . . . . . .$180.00
C2523H 20 tubes x 0.05 ml . . . . . . .$166.00 C3016H 20 tubes x 0.05 ml . . . . . . .$166.00
T7 Express Crystal Competent E. coli
C2523I 6 tubes x 0.2 ml . . . . . . .$130.00 C3016I 6 tubes x 0.2 ml . . . . . . .$130.00
For crystallography experiments
NEB Express l q Competent E. coli T7 Express lysY Competent E. coli T7 Express Crystal Competent E. coli
Control of IPTG-induced protein expression Tight control by inhibition of T7 RNA Pol (High Efficiency)
NEB Express l q Competent E. coli T7 Express lysY Competent E. coli C3022H 20 tubes x 0.05 ml . . . . . . .$206.00
(High Efficiency) (High Efficiency) C3022I 6 tubes x 0.2 ml . . . . . . .$160.00
C3037H 20 tubes x 0.05 ml . . . . . . .$166.00 C3010H 20 tubes x 0.05 ml . . . . . . .$166.00
C3037I 6 tubes x 0.2 ml . . . . . . .$130.00 C3010I 6 tubes x 0.2 ml . . . . . . .$130.00
T7 Express Competent E. coli T7 Express lysY/l q Competent E. coli
Most popular T7 expression strain Highest level of expression control
T7 Express Competent E. coli (High Efficiency) T7 Express lysY/l q Competent E. coli
www.neb.com
C2566H 20 tubes x 0.05 ml . . . . . . .$166.00 (High Efficiency)
800-632-5227
C2566I 6 tubes x 0.2 ml . . . . . . .$130.00 C3013H 20 tubes x 0.05 ml . . . . . . .$166.00
C3013I 6 tubes x 0.2 ml . . . . . . .$130.00
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