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Biomolecules for research MATERIAL SAFETY DATA SHEET
Section 1 : Substance Identification
BIOMOL Carboxyfluorescein Multi-Caspase Activity Kit for Apoptosis
Name:
Detection
Catalog Number: AK-117
Company Identification: BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting PA 19462-1202 USA
(610) 941-0430 (9am-5pm ET)
Section 2 : Components
Vial # Name: Contents: Quantity
KI-185 FAM-VAD-FMK Reagent Solid 1 vial
*See Attached MSDS for this reagent
KI-186 10X Wash Buffer Liquid 2 x 30 ml
*See attached MSDS for this reagent
KI-187 Fixative Liquid 2 x 3 ml
*See attached MSDS for this reagent
KI-188 Propidium Iodide Liquid 1 ml
*See attached MSDS for this reagent
KI-189 Hoechst Stain 200碌g/ml 1 vial
AK-117 Instruction Booklet 1 booklet
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Biomolecules for research MATERIAL SAFETY DATA SHEET
Section 1 : Substance Identification
FAM-VAD-FMK Reagent
Name:
Component Number: KI-185
Chemical Name: Carboxyfluorescein derivative of valylalayl aspartic acid fluoromethyl ketone
Chemical Class: Peptide derivative
Company Identification: BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting PA 19462-1202 USA
(610) 941-0430 (9am-5pm ET)
Section 2 : Health Hazard Data
Acute Health Effects: May be harmful if ingested, inhaled, or absorbed through the skin. May cause
irritation of the eyes, skin, and respiratory system. To the best of our knowledge, the toxicological
properties of this compound have not been fully investigated. Exercise appropriate precautions to
prevent opportunities for inhalation, and to prevent direct contact with skin and eyes. All
uncharacterized chemicals should be treated as suspected toxins.
Chronic Health Effects: unknown
Section 3 : First Aid Measures
Eye Contact: Check for and remove contact lenses. Flush eyes with running water for at least 15
minutes separating eyelids. Seek medical attention immediately.
Skin Contact: Wash with soap and water for 15 minutes. Remove contaminated clothing and shoes.
Seek medical attention immediately.
Inhalation: Remove from exposure. If breathing is difficult, administer oxygen. If breathing stops,
administer artificial respiration. Seek medical attention immediately. Provide chemical label
and MSDS if possible.
Ingestion: Remove dentures and clear mouth. If person is conscious, rinse mouth with water. Call
physician or poison control immediately. Provide chemical label and MSDS information if
possible.
Section 4 : Fire and Explosion Data
Flammability: Not Available
Flash Point: Not Available
Combustion Products: Not Available
Extinguishing Media: Carbon Dioxide, Dry chemical powder, polymer foam, water spray
Special Firefighting Procedures: Use self-contained breathing apparatus and protective clothing to
prevent contact with skin and eyes.
Unusual Fire/Explosion Hazards: None known
Section 5 : Spill or Leak Procedures
Cleanup Procedures: Wearing appropriate protective gear as outlined under 鈥淧rotective equipment鈥?
wipe up spill and place in sealed container and hold for disposal. Avoid raising dust. Ventilate
the area and wash spill site after material has been removed.
Waste Disposal Method: Observe all Federal, State and Local regulations concerning the disposal of
this product.
Section 6 : Storage and Handling Precautions
Protective Equipment: For spill clean up, wear suitable protective clothing, chemical resistant rubber
gloves, rubber boots, and chemical safety goggles.
Storage and Handling: This product should be stored at 2-8掳C. It should be kept in a tightly
closed container and protected from light. This product should be handled only by qualified
experienced professionals. Wash thoroughly after handling this material.
Section 7 : Physical Data
Appearance: Brown solid Odor: Not Available
Molecular Weight: Not Available Taste: Not Available
Boiling Point: Not Available Melting Point: Not Available
Solubility: Not Available Vapor Density: Not Available
Specific Gravity: Not Available Vapor Pressure: Not Available
Volatility: Not Available
Section 8 : Stability and Reactivity Data
Stability: This material is stable as supplied for 1 year if stored as directed
Conditions to Avoid: Exposure to Light
Incompatibles: Not Available
Section 9 : Toxicity Data
RTECS#: Not Available
Section 10 : Ecological Data
Ecotoxicological Information: None Available
The above information is believed to be correct but does not purport to be complete and should be used only as a guide. The burden of
safe use of this material rests entirely with the user.
Emergency Contact:
BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting, PA 19462 USA
(610) 941-0430
Last Revised : 1/7/04
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Biomolecules for research MATERIAL SAFETY DATA SHEET
Section 1 : Substance Identification
Name: 10X Apoptosis Wash Buffer
Component Number: KI-186
Company Identification: BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting PA 19462-1202 USA
(610) 941-0430 (9am-5pm ET)
Caution: This component contains 0.5% Sodium Azide
Section 2 : Health Hazard Data
Acute Health Effects: May be harmful if ingested, inhaled, or absorbed through the skin. May cause
irritation of the eyes, skin, and respiratory system. Diluted solutions are less toxic. This
component contains 0.5% sodium azide. Symptoms of overexposure may include : headache,
dizziness, gastrointestinal irritation, blurred vision, nausea, vomiting, low blood pressure, slow
heart rate, reduced body pH and temperature, convulsions or unconsciousness. To the best of our
knowledge, the toxicological properties of this mixture have not been fully investigated. Exercise
appropriate precautions to prevent opportunities for inhalation, and to prevent direct contact with
skin and eyes. Do not ingest.
Chronic Health Effects: Unknown
Section 3 : First Aid Measures
Eye Contact: Check for and remove contact lenses. Flush eyes with running water for at least 15
minutes separating eyelids. Seek medical attention immediately.
Skin Contact: Wash with soap and water for 15 minutes. Remove contaminated clothing and shoes.
Seek medical attention immediately.
Inhalation: Remove from exposure. If breathing is difficult, administer oxygen. If breathing stops,
administer artificial respiration. Seek medical attention immediately. Provide chemical label
and MSDS if possible.
Ingestion: Remove dentures and clear mouth. If person is conscious, give large amounts of water, and
induce vomiting. If person is unconscious, await assistance. Call physician or poison control
immediately. Provide chemical label and MSDS information if possible.
Section 4 : Fire and Explosion Data
Flammability: Not Flammable
Flash Point: Not Available
Combustion Products: Hazardous decomposition products such as hydrozoic fumes, and oxides of
phosphorus, sodium and nitrogen may be present.
Extinguishing Media: Carbon Dioxide, Dry chemical powder, polymer foam, water spray
Special Firefighting Procedures: Use self-contained breathing apparatus and protective clothing to
prevent contact with skin and eyes.
Unusual Fire/Explosion Hazards: None known
Section 5 : Spill or Leak Procedures
Cleanup Procedures: Wearing appropriate protective gear as outlined under 鈥淧rotective equipment鈥?
wipe up spill and place in sealed container and hold for disposal. For large spills, collect liquid in a
appropriate container or absorb with an inert material (such as vermiculite, dry sand, or charcoal)
and place in an appropriate waste container. Ventilate the area and wash spill site after material has
been removed.
Waste Disposal Method: Observe all Federal, State and Local regulations concerning the disposal of
this product.
Section 6 : Storage and Handling Precautions
Protective Equipment: For spill clean up, wear suitable protective clothing, chemical resistant rubber
gloves, rubber boots, and chemical safety goggles. Self contained breathing apparatus or
NIOSH/MSHA approved respirator is recommended to keep employee exposures below airborne
exposure limits.
Storage and Handling: This product should be stored at 2-8掳C. It should be kept in a tightly
closed container . This product should be handled only by qualified experienced professionals.
Wash thoroughly after handling this material.
Section 7 : Physical Data
Appearance: Slightly yellow liquid Odor: Slight
Molecular Weight: Not Available Taste: Not Available
Boiling Point: Not Available Melting Point: Not Available
Solubility: Water Vapor Density: Not Available
Specific Gravity: Not Available Vapor Pressure: Not Available
Volatility: Not Available pH: 6.8-7.0
Section 8 : Stability and Reactivity Data
Stability: This material is stable as supplied for 1 year if stored as directed
Conditions to Avoid: Not Available
Incompatibles: Sodium Azide is incompatible with strong acids and oxidizers.
Section 9 : Toxicity Data
RTECS#: Not Available
Section 10 : Ecological Data
Ecotoxicological Information: None Available
The above information is believed to be correct but does not purport to be complete and should be used only as a guide. The burden of
safe use of this material rests entirely with the user.
Emergency Contact:
BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting, PA 19462 USA
(610) 941-0430
Last Revised : 1/7/04
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Biomolecules for research MATERIAL SAFETY DATA SHEET
Section 1 : Substance Identification
Fixative
Name:
Component Number: KI-187
Company Identification: BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting PA 19462-1202 USA
(610) 941-0430 (9am-5pm ET)
Caution: This component contains 10% Formaldehyde
Section 2 : Health Hazard Data
Acute Health Effects: May be harmful if ingested, inhaled, or absorbed through the skin. May cause
irritation of the eyes, skin, and respiratory system. This component contains formaldehyde which
is a potential cancer hazard. Prolonged or repeated exposure increases the risk. Eye contact may
cause permanent eye damage. This component also contains methanol. Methanol is toxic if
ingested, inhaled, or absorbed through the skin. Ingestion of methanol may cause blindness.
Methanol is a strong sensitizer, and may cause allergic reactions. To the best of our knowledge,
the toxicological properties of this mixture have not been fully investigated. Exercise appropriate
precautions to prevent opportunities for inhalation, and to prevent direct contact with skin and eyes.
Do not ingest.
Chronic Health Effects: Formaldehyde exposure has been linked to cancers of the lungs, vasopharynx,
oropharynx and nasal passages.
Section 3 : First Aid Measures
Eye Contact: Check for and remove contact lenses. Flush eyes with running water for at least 15
minutes separating eyelids. Seek medical attention immediately.
Skin Contact: Wash with soap and water for 15 minutes. Remove contaminated clothing and lauder
immediately. Throw away contaminated shoes.
Seek medical attention.
Inhalation: Remove from exposure. If breathing is difficult, administer oxygen. If breathing stops,
administer artificial respiration. Seek medical attention immediately. Provide chemical label
and MSDS if possible.
Ingestion: Remove dentures and clear mouth. If person is conscious, give large amounts of water, milk,
or activated charcoal. If person is unconscious, await assistance. Call physician or poison
control immediately. Provide chemical label and MSDS information if possible.
Section 4 : Fire and Explosion Data
Flammability: Flammable as supplied
Flash Point: Not Available
Combustion Products: Not Available
Extinguishing Media: Carbon Dioxide, Dry chemical powder, 鈥渁lcohol鈥? foam
Special Firefighting Procedures: Use self-contained breathing apparatus and protective clothing to
prevent contact with skin and eyes.
Unusual Fire/Explosion Hazards: This component is a moderate fire hazard, and a moderate explosion
hazard. Avoid heat, sparks, and open flame. Formaldehyde reacts with nitrogen dioxide,
nitromethane, perchloric acid, and aniline, or peroxyformic acid to yield explosive compounds.
Section 5 : Spill or Leak Procedures
Cleanup Procedures: Wearing appropriate protective gear as outlined under 鈥淧rotective equipment鈥?
wipe up spill and place in sealed container and hold for disposal. Ventilate the area and wash spill
site after material has been removed. Eliminate ignition sources from the spill area.
Waste Disposal Method: Observe all Federal, State and Local regulations concerning the disposal of
this product.
Section 6 : Storage and Handling Precautions
Protective Equipment: Use only in a chemical fume hood, or with equivalent ventilation conditions.
For spill clean up, wear suitable protective clothing, chemical resistant rubber
gloves, rubber boots, and chemical safety goggles. Self contained breathing apparatus or
NIOSH/MSHA approved respirator is required.
Storage and Handling: This product should be stored at room temperature. It should be kept in a tightly
closed container and protected from light. This product should be handled only by qualified
experienced professionals. Wash thoroughly after handling this material.
Section 7 : Physical Data
Appearance: Clear Liquid Odor: Moderately pungent odor
Molecular Weight: Not Available Taste: Not Available
Boiling Point: Not Available Melting Point: Not Available
Solubility: Not Available Vapor Density: Not Available
Specific Gravity: Not Available Vapor Pressure: Not Available
Volatility: Not Available pH: 7.3-7.7
Section 8 : Stability and Reactivity Data
Stability: This material is stable as supplied for 1 year if stored as directed
Conditions to Avoid: Protect from heat, sparks, or open flame.
Incompatibles: Strong acids, oxidizers, isocyanates, strong alkalines, phenol, anhydrides, oxides, and
inorganic acids. Contact with HCL may cause the formation of bischloromethyl ether which is a
potent carcinogen.
Section 9 : Toxicity Data
RTECS#: Not Available
Section 10 : Ecological Data
Ecotoxicological Information: None Available
The above information is believed to be correct but does not purport to be complete and should be used only as a guide. The burden of
safe use of this material rests entirely with the user.
Emergency Contact:
BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting, PA 19462 USA
(610) 941-0430
Last Revised : 1/7/04
庐
Biomolecules for research MATERIAL SAFETY DATA SHEET
Section 1 : Substance Identification
Propidium Iodide
Name: CAS Number: 25535-16-4
Component Number: KI-188 Chemical Formula: C27H34I2N4
Chemical Class: Polycyclic aromatic
Company Identification: BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting PA 19462-1202 USA
(610) 941-0430 (9am-5pm ET)
Section 2 : Health Hazard Data
Acute Health Effects: May be harmful if ingested, inhaled, or absorbed through the skin. May cause
irritation of the eyes, skin, mucous membranes, or upper respiratory tract.
Chronic Health Effects: There is limited evidence that Propidium iodide is mutagenic in laboratory
cell cultures. Until further testing has been done, this compound should be treated as a possible
mutagen in humans
Section 3 : First Aid Measures
Eye Contact: Check for and remove contact lenses. Flush eyes with running water for at least 15
minutes separating eyelids. Seek medical attention immediately.
Skin Contact: Wash with soap and water for 15 minutes. Remove contaminated clothing and shoes.
Seek medical attention immediately.
Inhalation: Remove from exposure. If breathing is difficult, administer oxygen. If breathing stops,
administer artificial respiration. Seek medical attention immediately. Provide chemical label
and MSDS if possible.
Ingestion: Remove dentures and clear mouth. If person is conscious, rinse mouth with water. Call
physician or poison control immediately. Provide chemical label and MSDS information if
possible.
Section 4 : Fire and Explosion Data
Flammability: Not Flammable as supplied in solution in de-ionized H2O
Flash Point: Not Available
Combustion Products: Carbon monoxide, carbon dioxide, nitrogen oxides, hydrogen iodide.
Extinguishing Media: Carbon Dioxide, Dry chemical powder, polymer foam, water spray
Special Firefighting Procedures: Use self-contained breathing apparatus and protective clothing to
prevent contact with skin and eyes.
Unusual Fire/Explosion Hazards: None known
Section 5 : Spill or Leak Procedures
Cleanup Procedures: Wearing appropriate protective gear as outlined under 鈥淧rotective equipment鈥?
wipe up spill and place in sealed container and hold for disposal. Ventilate the area and wash spill
site after material has been removed.
Waste Disposal Method: Observe all Federal, State and Local regulations concerning the disposal of
this product.
Section 6 : Storage and Handling Precautions
Protective Equipment: For spill clean up, wear suitable protective clothing, chemical resistant rubber
gloves, rubber boots, and chemical safety goggles. Self contained breathing apparatus or
NIOSH/MSHA approved respirator is required.
Storage and Handling: This product should be stored at 2-8掳C and protected from light. It should be
kept in a tightly closed container. This product should be handled only by qualified experienced
professionals. Wash thoroughly after handling this material.
Section 7 : Physical Data
Appearance: Light pink solution Odor: None
Molecular Weight: 668.4 Taste: Not Available
Boiling Point: Not Available Melting Point (solid): 224-228掳C
Solubility: Water Vapor Density: Not Available
Specific Gravity: Not Available Vapor Pressure: Not Available
Volatility: Not Available pH: 6.7-7.2
Section 8 : Stability and Reactivity Data
Stability: This material is stable if stored as directed
Conditions to Avoid: None known
Incompatibles: Strong oxidizing agents
Section 9 : Toxicity Data
RTECS#: SF7949600
SC LD50: Mouse : 16mg/kg (British J. Pharmacol. Chemother. 1956 11, 334)
Section 10 : Ecological Data
Ecotoxicological Information: None Available
The above information is believed to be correct but does not purport to be complete and should be used only as a guide. The burden of
safe use of this material rests entirely with the user.
Emergency Contact:
BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting, PA 19462 USA
(610) 941-0430
Last Revised : 1/7/04
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Biomolecules for research MATERIAL SAFETY DATA SHEET
Section 1 : Substance Identification
Name: Hoechst Stain
Component Number: KI-189
Company Identification: BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting PA 19462-1202 USA
(610) 941-0430 (9am-5pm ET)
Caution: This component contains Hoechst 33342 stain reagent at 200碌g/ml in DI H2O
Section 2 : Health Hazard Data
Acute Health Effects: May be harmful if ingested, inhaled, or absorbed through the skin. May cause
irritation of the eyes, skin, and respiratory system. This component contains Hoechst 33342 ,
which exhibits mutagenic properties as demonstrated by DNA damage to hamster lung cells and
mouse fibroblast cells at 1碌M/l and 5碌M/l respectively. Hoechst 33342 is not currently listed as a
carcinogen by NTP, IARC or OSHA but should be treated as such. Symptoms of exposure are
undefined, but this staining reagent should only be handled using appropriate protective measures.
Exercise appropriate precautions to prevent opportunities for inhalation, and to prevent direct
contact with skin and eyes. Do not ingest.
Chronic Health Effects: Unknown 鈥? Possible Carcinogen
Section 3 : First Aid Measures
Eye Contact: Check for and remove contact lenses. Flush eyes with running water for at least 15
minutes separating eyelids. Seek medical attention immediately.
Skin Contact: Wash with soap and water for 15 minutes. Remove contaminated clothing and shoes.
Seek medical attention immediately.
Inhalation: Remove from exposure.. Seek medical attention immediately. Provide chemical label
and MSDS if possible.
Ingestion: Remove dentures and clear mouth. . Call physician or poison control immediately.
Provide chemical label and MSDS information if possible.
Section 4 : Fire and Explosion Data
Flammability: Not Flammable
Flash Point: Not Available
Combustion Products: Not Available.
Extinguishing Media: Carbon Dioxide, Dry chemical powder, polymer foam, water spray
Special Firefighting Procedures: Use self-contained breathing apparatus and protective clothing to
prevent contact with skin and eyes.
Unusual Fire/Explosion Hazards: None known
Section 5 : Spill or Leak Procedures
Cleanup Procedures: Wearing appropriate protective gear as outlined under 鈥淧rotective equipment鈥?
wipe up spill and place in sealed container and hold for disposal. Ventilate the area and wash spill
site after material has been removed.
Waste Disposal Method: Observe all Federal, State and Local regulations concerning the disposal of
this product.
Section 6 : Storage and Handling Precautions
Protective Equipment: For spill clean up, wear suitable protective clothing, chemical resistant rubber
gloves, rubber boots, and chemical safety goggles. Self contained breathing apparatus or
NIOSH/MSHA approved respirator is recommended.
Storage and Handling: This product should be stored at 2-8掳C and protected from light. It should be
kept in a tightly closed container . This product should be handled only by qualified experienced
professionals. Wash thoroughly after handling this material.
Section 7 : Physical Data
Appearance: Pale green liquid Odor: None
Molecular Weight: Not Available Taste: Not Available
Boiling Point: Not Available Melting Point: Not Available
Solubility: Water Vapor Density: Not Available
Specific Gravity: Not Available Vapor Pressure: Not Available
Volatility: Not Available pH: 3.9-4.4
Section 8 : Stability and Reactivity Data
Stability: This material is stable as supplied for 1 year if stored as directed
Conditions to Avoid: Exposure to light
Incompatibles: None Known.
Section 9 : Toxicity Data
RTECS#: Not Available
Section 10 : Ecological Data
Ecotoxicological Information: None Available
The above information is believed to be correct but does not purport to be complete and should be used only as a guide. The burden of
safe use of this material rests entirely with the user.
Emergency Contact:
BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting, PA 19462 USA
(610) 941-0430
Last Revised : 1/7/04
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