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Biomolecules for research MATERIAL SAFETY DATA SHEET
Section 1 : Substance Identification
HDAC FluorimetricCellular Activity Assay
Name:
A Fluor de Lys Fluorescent Assay System
Catalog Number: AK-503
Company Identification: BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting PA 19462-1202 USA
(610) 941-0430 (9am-5pm ET)
Section 2 : Components
Vial # Name: Contents: Quantity
KI-345 Nuclear Extract from HeLa Cells 2 mg protein / ml in 0.1 M KCl, 50碌l
(human cervical cancer cell line) 20 mM HEPES/NaOH, pH 7.9, 20%
(v/v) glycerol, 0.2 mM EDTA, 0.5 mM
DTT, 0.5 mM PMSF.
Fluor de Lys鈩? Substrate
KI-104 50 mM in DMSO 50碌l
Fluor de Lys鈩? Developer Concentrate
KI-105 20X Stock Solution 300碌l
(20 X)
GR309-9090 Trichostatin A 0.2 mM in DMSO 100碌l
(HDAC Inhibitor)
*See Attached MSDS for this component
KI-283 Nicotinamide 50 mM in Assay Buffer , 500 碌l
(Sirtuin Inhibitor)
Fluor de Lys鈩? Deacetylated Standard
KI-142 10 mM in DMSO 30碌l
KI-143 HDAC Assay Buffer 25 mM Tris/Cl, ph 8.0, 137 mM 20ml
NaCl, 2.7 mM KCl, 1 mM MgCl2
KI-346 Cell Lysis Buffer 1.0% NP-40 in Assay Buffer 20 ml
AK503 Instruction Booklet 1 booklet
KI-101 陆 Volume Microplate 陆 Volume 96-well plate 1 plate
KI-347 Sterile 陆 Volume Microplate with Lid 1 set
Section 3 : Health Hazards
Please be advised that the physical, chemical and toxicological properties of many of these components
have not yet been fully investigated. It is therefore advised that all laboratory personnel handling this
product follow standard laboratory safety procedures. It is recommended that personnel wear safety
goggles, gloves, and protective clothing at all times, and avoid direct contact with the reagents supplied in
this kit.
Section 4 : First Aid
Eye Contact: Check for and remove contact lenses. Flush eyes with running water for at least 15
minutes separating eyelids. Seek medical attention immediately
Skin Contact: Wash with soap and water for 15 minutes. Remove contaminated clothing and shoes.
If irritation or discomfort develops, seek medical attention.
Inhalation: Remove from exposure. If breathing is difficult, administer oxygen. If breathing stops,
administer artificial respiration. Seek medical attention if patient feels unwell or develops any
respiratory symptoms. Provide chemical label and MSDS if possible.
Ingestion: Remove dentures and clear mouth. If person is conscious, rinse mouth with water. Call
physician or poison control immediately. Provide chemical label and MSDS information if
possible.
Section 5 : Fire / Flammability Information
None of the components of this kit pose a significant risk in case of fire. Fire fighting media should be
selected to suit other materials involved in the fire. It is recommended that firefighters wear protective gear
and self contained breathing apparatus to limit exposure.
Section 6 : Spill or Leak Procedures.
Cleanup Procedures: Wearing appropriate protective gear as outlined under 鈥淧rotective equipment鈥?
wipe up spill area and rinse residue down the drain with plenty of water. Wash spill site with soap
and water.
Waste Disposal Method: Dilute waste solutions and reagents with water and dispose of in accordance
with federal, state, and local regulations.
Section 7 : Storage and Handling Precautions
Protective Equipment: For spill clean up, wear suitable protective clothing, chemical resistant rubber
gloves, rubber boots, and chemical safety goggles.
Storage and Handling: The components of this kit should be stored 鈥?70掳C with the exception of the
96-well plates, and the instruction booklet which can be stored at room temperature. All
components should be kept in tightly closed containers. This product should be handled only by
qualified experienced professionals. Wash thoroughly after handling the materials included in this
kit.
Section 8 : Stability and Reactivity Data
Stability: This material is stable if stored as directed
Conditions to Avoid: None known
Incompatibles: Not available
Section 10 : Ecological Data
Ecotoxicological Information: None Available
The above information is believed to be correct but does not purport to be complete and should be used only as a guide. The burden of
safe use of this material rests entirely with the user.
Emergency Contact:
BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting, PA 19462 USA
(610) 941-0430
Last Revised : 7/21/06
庐
Biomolecules for research MATERIAL SAFETY DATA SHEET
Section 1 : Substance Identification
Trichostatin A
Name: CAS Number: 58880-19-6
Component Number: GR309-9090 Chemical Formula: C17H22N2O3
Chemical Name: 7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl]-7-oxo-2,4-heptadienamide
Chemical Class: Hydroxamic acid
Company Identification: BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting PA 19462-1202 USA
(610) 941-0430 (9am-5pm ET)
Section 2 : Health Hazard Data
Acute Health Effects: May be harmful if ingested, inhaled, or absorbed through the skin. May be
irritating to skin, eyes, nose, throat, and lungs. May cause allergic reactions in certain
hypersensitive individuals. Symptoms of overexposure can include: headache, dizziness, anorexia,
stomach ache, diarrhea, nausea, vomiting or fever.
Chronic Health Effects: unknown
Section 3 : First Aid Measures
Eye Contact: Check for and remove contact lenses. Flush eyes with running water for at least 15
minutes separating eyelids. Seek medical attention immediately.
Skin Contact: Wash with soap and water for 15 minutes. Remove contaminated clothing and shoes.
Seek medical attention immediately.
Inhalation: Remove from exposure. If breathing is difficult, administer oxygen. If breathing stops,
administer artificial respiration. Seek medical attention immediately. Provide chemical label
and MSDS if possible.
Ingestion: Remove dentures and clear mouth. If person is completely conscious, give plenty of milk or
water and induce vomiting. Call physician or poison control immediately. Provide chemical
label and MSDS information if possible.
Section 4 : Fire and Explosion Data
Flammability: Not Available
Flash Point: Not Available
Combustion Products: Carbon monoxide, Carbon dioxide, Nitrogen oxides.
Extinguishing Media: Carbon Dioxide, Dry chemical powder, polymer foam, water spray
Special Firefighting Procedures: Use self-contained breathing apparatus and protective clothing to
prevent contact with skin and eyes.
Unusual Fire/Explosion Hazards: None known
Section 5 : Spill or Leak Procedures
Cleanup Procedures: EVACUATE AREA Wearing appropriate protective gear as outlined under
鈥淧rotective equipment鈥? wipe up spill and place in sealed container and hold for disposal. Avoid
raising dust. Ventilate the area and wash spill site after material has been removed.
Waste Disposal Method: Observe all Federal, State and Local regulations concerning the disposal of
this product.
Section 6 : Storage and Handling Precautions
Protective Equipment: For spill clean up, wear suitable protective clothing, chemical resistant rubber
gloves, rubber boots, and chemical safety goggles. Self contained breathing apparatus or
NIOSH/MSHA approved respirator is required.
Storage and Handling: This product should be stored at 鈥?20掳C. It should be kept in a tightly
closed container. This product should be handled only by qualified experienced professionals.
Wash thoroughly after handling this material.
Section 7 : Physical Data
Appearance: White solid Odor: Not Available
Molecular Weight: 302.4 Taste: Not Available
Boiling Point: Not Available Melting Point: 150-151掳C
Solubility: DMSO, Ethanol, DMF Vapor Density: Not Available
Specific Gravity: Not Available Vapor Pressure: Not Available
Volatility: Not Available
Section 8 : Stability and Reactivity Data
Stability: This material is stable if stored as directed
Conditions to Avoid: Excessive exposure to sunlight or heat
Incompatibles: Oxidizing agents
Section 9 : Toxicity Data
RTECS#: Not Listed
Section 10 : Ecological Data
Ecotoxicological Information: None Available
The above information is believed to be correct but does not purport to be complete and should be used only as a guide. The burden of
safe use of this material rests entirely with the user.
Emergency Contact:
BIOMOL International L.P.
5120 Butler Pike
Plymouth Meeting, PA 19462 USA
(610) 941-0430
Last Revised : 7/21/06
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