Operating Instructions
863 Valley View Road, Eighty Four PA 15330 USA
Tel: 724-941-9701 Fax: 724-941-1369 e-mail: skctech@skcinc.com
BioStage庐 Impactors
The SKC BioStage single-stage impactors operate on the principle of inertial
impaction and meet NIOSH Method 0800 and 0801 requirements and ACGIH
recommendations for sampling viable microorganisms including bacteria and
fungi. Made of precision-tooled autoclavable aluminum, sampler components
are precision-threaded together for a positive seal. Air is drawn through the
impactor where particles are impacted onto an agar collection medium. Two
models of the BioStage Impactor are available as follows:
Standard BioStage contains a 400-hole jet classification stage
and is operated at 28.3 L/min
BioStage 200 contains a 200-hole jet classification stage and is
operated at 14.15 L/min
Specifications
Standard BioStage: 28.3 L/min
Flow Rate:
14.15 L/min
BioStage 200:
Precision-tooled autoclavable aluminum
Material:
Standard BioStage: 400 holes
Jet Classification Stage:
200 holes
BioStage 200:
90- to 100-mm agar plate*
Sample Media:
Colony culture鈥?
Analysis:
* Consult laboratory for information on appropriate agar choice.
鈥? In situations where spore counts are high, positive hole correction should be used. See Macher,
J., "Positive Hole Correction of Multiple-jet Impactors for Collecting Viable Microorganisms,"
American Industrial Hygiene Journal, 50 (11), 1989, pp. 561-568. Abstract available at http://
taylorandfrancis.metapress.com/. Search on "positive hole correction."
Sample Media
Use appropriate agar in a 90- to 100-mm agar plate with the Standard BioStage
or the BioStage 200. Plastic or glass agar plates can be used. Verify with your
agar supplier that the plates contain the proper volume of agar to achieve the
appropriate agar height to maintain impactor cut-points.
Suggested Media
For bacteria: Tryptic Soy Agar (TSA) or Blood Agar Plates (BAP)
Potato Dextrose Agar (PDA), Malt Extract Agar (MEA),
For Fungi:
Dichloran Glycerol 18 Agar (DG-18), or Corn Meal Agar (CMA).
For information on laboratories that can provide agar plates and analyze
samples, see Laboratories at www.skcinc.com.
Caution: Sanitize hands and impactor any time contamination from
handling is possible. Do not touch holes in jet classification stage.
Assembly
1. Remove the inlet cone by lifting
it up and off.
2. Remove the jet classification
stage by gently unscrewing it
and lifting it up and off.
3. Remove the lid from an agar plate and place it on the
three raised metal pins in the base plate of the impactor.
Caution: Do not operate without an agar plate in
place.
4. Gently screw the jet classification stage back onto the
base plate. Align and press the inlet cone onto the jet
classification stage until a secure seal is established.
5. Connect the BioStage outlet to a sample pump with
tubing.
Calibration
1. Insert the calibration adapter* into the inlet cone. Use
flexible tubing to connect the inlet of the calibration
adapter to a primary flowmeter. See sample pump and
calibrator operating instructions for further calibration
information.
2. Calibrate sample pump to 28.3 L/min when using the
Standard BioStage or 14.15 L/min if
using the BioStage 200. Use the
rotameter* accessory to check flow in
the field.
* Calibration adapter and rotameter are for
use with both models of BioStage (see
Accessories).
Sampling
Caution: When sampling indoors, close all doors and windows that could
affect airflow in the sampling area.
1. Calibrate the flow rate (see Assembly).
2. Remove calibration adapter, tubing, and flowmeter.
3. Clean all surfaces of the impactor (see Cleaning).
4. Place a new, unexposed agar plate into the base plate (see Assembly). Remove
the lid from the agar plate. Reassemble the impactor.
5. Mount impactor on a tripod, if desired, by
screwing the threaded hole on the bottom
of the impactor onto the threaded top of the
tripod accessory (see Accessories).
6. Turn on the vacuum pump and sample for
2 to 5 minutes.
Caution: Sampling too long can cause
overgrowth of sample. Sampling times
that are too short can cause false
negatives.
BioStage mounted on QuickTake 30
7. After sampling is complete, turn off the
pump and disconnect the flexible tubing.
Removing the Agar Plate (Sample)
1. Remove the inlet cone and jet classification stage (see Assembly, Steps 1 and 2).
2. Remove the agar plate containing the sample and replace its cover
immediately.
3. Label the bottom of the agar plate with all pertinent sampling information.
Secure agar plate lid with tape and place in a sealable bag.
4. Place agar plate containing the sample in an ice chest with blue ice.
5. Send the agar plate containing the sample and a blank unexposed agar plate
to a laboratory according to laboratory instructions.
Note: Outdoor samples should be collected for comparison to indoor samples.
An indoor control sample should be taken also for non-complaint areas.
Clearly mark each sample.
Caution: Sanitize hands and the BioStage Impactor in between samples.
Caution: Never use agar that has expired, displays visible cracks, or has been
contaminated.
Cleaning
Disassemble and place parts in an ultrasonic bath with a mild detergent-water
solution. Thoroughly rinse and air dry in a dust-free space.
Sterilizing
Autoclave or immerse in ethyl alcohol and air dry. In the field, parts can be
swabbed with alcohol on a sterile gauze pad and air dried.
Ordering Information
BioStage Cat. No.
Standard BioStage鈥? single-stage bioaerosol impactor
225-9611
(400 holes, 28.3 L/min)
BioStage 200鈥? single-stage bioaerosol impactor
225-9610
(200 holes, 14.15 L/min)
BioStage Pump Kit鈥? - AC includes Standard BioStage
Sampler, vacuum pump (Gast 1532) with rotameter,
225-9535K
tubing, tripod stand, and carry case
BioStage Pump Kit鈥? - DC includes Standard BioStage
Sampler, QuickTake 30 pump with battery, AC charger/
adapter, mounting bracket with inlet adapter, field
228-9530K
rotameter, and deluxe carry case.
BioStage 200 Pump Kit鈥? includes BioStage 200鈥?,
QuickTake 15 pump, rotameter, tripod stand, tubing, and
225-9610K
carry case
Accessories Cat. No.
Calibration Adapter for BioStage, allows tubing to
connect to BioStage inlet
Suitable for both BioStage models 225-9537
Tripod Stand, telescopes to 5 ft. to hold BioStage in
225-9536
breathing area (does not hold pump)
Mounting Bracket for QuickTake 30, holds BioStage in
228-9531
place on pump during sampling
鈥?
Requires microbiological media supplied by analytical laboratories. For lab list, go to
www.skcinc.com.
BioStage on
Tripod Stand with pump
Pump Kit #225-9535K
www.skcinc.com
Notice: This operating instruction may not address all safety concerns (if any) associated
with this product and its use. The user is responsible for determining and following the
appropriate safety and health practices and regulatory limitations (if any) before using the
product. The information contained in this document should not be construed as legal advice,
opinion, or as a final authority on legal or regulatory procedures.
Form #40083 Rev 0510
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