1
Material Safety Data Sheet
According to 91/155 EEC
This Material Safety Data Sheet applies to all the Diaclone Dual Color Eli-spot PVDF Enzymatic kits
1/ Data on components
Regarding components comprised in the kit, the following products are defined as hazardous chemical according the
Directive 67/548/EEC
Accordingly, Material Safety Data Sheet(s) for the following chemical(s) are enclosed:
Fluorescein Isothiocyanate Isomer I: is used to label FITC conjugated detection antibody.
BCIP / NBT is used as liquid alkaline phosphatase substrate system.
AEC: is used as HRP substrate.
Sodium Acetate : is used in AEC buffer.
Other chemical contained within these products do not meet definition of a hazardous chemical according EC
Directives.
Note: BSA Bovine Serum Albumine from a certified BSE-free nothamerican source is comprised in the kit.
2/ General information
The above information is believed to be correct but does not purport to be al inclusive and shall be used only as a
guide. DIACLONE SAS shall not be liable for any damages resulting from handling or from contact with the above
products.
Section 1 - Identification of substance
Product Identification: Dual Color Eli-spot PVDF Enzymatic kits
FITC conjugated detection antibody
Manufacturer: DIACLONE SAS
1, Bd Fleming
F-25000 Besançon
France
Tel: +33 3 81 41 38 38
Fax: +33 3 81 41 36 36
e-mail: info@diaclone.com
www.diaclone.com
Section 2 - Data on components
Chemical characterization: Preparation
Hazardous ingredients: % CAS Number EC Number
Fluorescent Isothiocyanate Isomer I < 5% 3326-32-7 222-042-0
R 20-21-22/36-37-38/42-43; S 26
Additional information:
The data and advice relate to the concentrations not of the lyophilized powder, but of the prepared solution
Section 3 - Hazard Identification
Hazard Description: not applicable
Information concerning to particular hazards to man and environment: not applicable
Section 4 - First Aid Measures
After inhalation Supply fresh air; consult doctor in case of complaints
After skin contact Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated
cothing and shoes.
After eye contact Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper
and lower lid.
After ingestion Allow the victim to rinse his mouth and then drink 2-4 cupfuls of water, and seek medical advice.
Approved by / Approuvé par
Issue N°1 July,23 2002 S.Thomas / J. Wijdenes
2
Material Safety Data Sheet
According to 91/155 EEC
Section 5 - Fire Fighting Measures
Suitable extinguishing agents
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipement No special measures required
Section 6 - Accidental Release Measures
Person-related safety precautions: Wear protective equipment. Keep unprotected persons away.
Measures for environmental protections
Prevent seepage into sewage system, workpits and cellars.
Dilute with plenty of water.
Do not allow to enter sewers/ surface or ground water.
Measures for cleaning / collecting
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Use neutralizing agent.
Dispose contaminated material as waste according to section 13.
Ensure adequate ventilation.
Section 7- Handling and storage
Handling:
Information for safe handling: Avoid contact with eyes, skin and clothing. Keep container tightly closed. Use with
Information about fire - and explosion protection:
The product listed in this MSDS contains sodium azide, a chemical highly toxic in pure form. At product
Storage:
Requirement to be met by storerooms and receptables: Store in a cool, dry, well-ventilated area.
Information about storage in one common storage facility: Keep in tightly closed container.
Further information about storage conditions: None
Storage class:
Class according to regulation on flammable liquids: void
Section 8 - Exposure Controls / Personal Protection
Additional information about design of technical facilities: No further data; see section 7
Ingredients with limit values that require monitoring at the workplace
The product does not contain any relevant quantities of material with critical values that have to be
monitored at the workplace.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals
Respiratory protection: Not required
Protection of hands: Protective gloves
Eye Protection: Goggles recommended during refilling
Section 9 - Physical and Chemical Properties
Form: Liquid
Colour: pale yellow
Odour: Odourless
Melting point/Melting range: N/A
Boiling point/Boiling range: N/A
Flash point: Not applicable
Autoignition temperature: N/A
Danger of explosion: Product does not present an explosion hazard
Densitiy: N/A
Solubility in / Miscibility with
water : fully miscible
Approved by / Approuvé par
Issue N°1 July,23 2002 S.Thomas / J. Wijdenes
3
Material Safety Data Sheet
According to 91/155 EEC
Section 10 - Stability and Reactivity
Thermal decomposition: No decomposition if used according to specifications
Reactivity and under what conditions: Incompatible with alcohols, bases, amines, acides.
Dangerous decomposition products:
Toxic fumes of carbon monoxide, carbon dioxide and nitrous oxides.
Section 11 - Toxicological Information
Route of entry: Skin Contact, Eye contact, Inhalation, Ingestion
Effects of acute exposure to Product:
Nocif in case of ingestion, inhlation or skin contact.
Irritant effect on the skin, and eyes.
Effects of chronic exposure to Product:
Long term exposure may cause asthma, nausea, dizzi and headache.
Exposure Limits Irritancy Sensitization To Product Carcinogenicity
N/A N/A N/A N/A
Terratogenicity Reproductive Toxicity Mutagenicity Synergistic Products
N/A N/A N/A N/A
Section 12 - Ecological Information
General Notes: Information not yet available
Section 13 - Disposal considerations
Waste disposal: dispose of in accordance with local official environmental regulations
Section 14 - Transport Information
Special shipping information: N/A
Section 15 - Regulations
Labelling according to European labelling guidelines:
Observe the general safety regulations when handling chemicals.
Section 16 - Other information
This information is prepared on our present knowledge. However, this shall not constitute a guarantee for any specific
product features and shall not establish a legally valid contractual relationship.
Prepared by: Marketing Department Phone N°:+33 3 81 41 38 38 Date: July 02
Approved by / Approuvé par
Issue N°1 July,23 2002 S.Thomas / J. Wijdenes
4
Material Safety Data Sheet
According to 91/155 EEC
Section 1 - Identification of substance
Product Identification: Dual Color Eli-spot PVDF Enzymatic kits
Ready-to-use BCIP/NBT substrate buffer
Manufacturer: DIACLONE SAS
1, Bd Fleming
F-25000 Besançon
France
Tel: +33 3 81 41 38 38
Fax: +33 3 81 41 36 36
e-mail: info@diaclone.com
www.diaclone.com
Section 2 - Data on components
Chemical characterization: Preparation
The following data is for the 100% chemical as no data is available on the solution.
Hazardous ingredients: % CAS Number EC Number
5-Bromo-4-chloro-3-indoxyl phosphate nitro 6578-06-9 298-83-9
Section 3 - Hazard Identification
Hazard Description: harmful
Irritating to eyes, repsiratory system and skin
Harmful if swallowed, inhalated or absorbed through skin.
Avoid contact with metals.
Air and light sensitive
Refrigerate for best storage
Section 4 - First Aid Measures
After skin contact: Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and
shoes.
After eye contact: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper
and lower lid.
After ingestion Do not induce vomiting; allow the victim to rinse his mouth and then drink 2-4 cupfuls of water, and
seek medical advice.
Section 5 - Fire Fighting Measures
Suitable extinguishing agents
Use dry chemical, carbon dioxyde or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipement No special measures required
Section 6 - Accidental Release Measures
Person-related safety precautions: Not required.
Measures for environmental protections:
Evacuate area and ventilate area.
Wear self-contained breathing apparatus, rubber boots and heavy rubber gloves. Absorb on sand or vermiculite and
place in closed containers for waste disposal.
Avoid splashing.
Section 7- Handling and storage
Refer to section 8.
Section 8 - Exposure Controls / Personal Protection
The usual precautionary measures are to be adhered to when handling chemicals.
Chemical safety goggles.
Use only in a chemical fume hood.
Compatible resistant gloves.
Avoid any skin or clothing contact.
Wash thoroughly after handling.
Keep tightly closed and refrigerate.
Approved by / Approuvé par
Issue N°1 July,23 2002 S.Thomas / J. Wijdenes
5
Material Safety Data Sheet
According to 91/155 EEC
Section 9 - Physical and Chemical Properties
Form: Liquid
Colour: Clear to very pale yellow solution
Odour: Odourless
Melting point/Melting range: 0°C (water)
Boiling point/Boiling range: 100°C (water)
Flash point: Not applicable
Autoignition temperature: N/A
Danger of explosion: Product does not present an explosion hazard
Densitiy: N/A
Solubility in / Miscibility with
water : fully miscible
Section 10 - Stability and Reactivity
Thermal decomposition: No decomposition if used according to specifications
Reactivity and under what conditions:
Incompatibles with bases, amines, alkali metals, copper, copper alloys
Hazardous decomposition products
Toxic fumes of carbon monoxyde, carbon dioxyde, nitrous oxydes, hydrogen chloride gas, hydrogen bromide gas,
phosphorous oxydes.
Section 11 - Toxicological Information
Route of entry: Skin Contact, Eye contact, Inhalation, Ingestion
Effects of acute exposure to Product:
Harmful if swallowed, inhaled or absorbed through skin. Cause eyes adn skin irritation.
Material is irritating and destructive to tissue of the mucous membranes and upper respiratory tract.
Effects of chronic exposure to Product:
To the best of our knowledge, the chemical, physical and toxicological properties have not been throughly tested.
Section 12 - Ecological Information
General Notes: N/A
Section 13 - Disposal considerations
Waste disposal:
Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equiped with an after burner
and scrubber.
Observe all local official environmental regulations.
Section 14 - Transport Information
Special shipping information:
Does not need to be shipped as hazardous.
Section 15 - Regulations
Labelling according to European labelling guidelines:
Observe the general safety regulations when handling chemicals.
Section 16 - Other information
This information is prepared on our present knowledge. However, this shall not constitute a guarantee for any specific
product features and shall not establish a legally valid contractual relationship.
Prepared by: Marketing Department Phone N°:+33 3 81 41 38 38 Date: July 02
Approved by / Approuvé par
Issue N°1 July,23 2002 S.Thomas / J. Wijdenes
6
Material Safety Data Sheet
According to 91/155 EEC
Section 1 - Identification of substance
Product Identification: Dual Color Eli-spot PVDF Enzymatic kits
AEC Substrate
Manufacturer: DIACLONE SAS
1, Bd Fleming
F-25000 Besançon
France
Tel: +33 3 81 41 38 38
Fax: +33 3 81 41 36 36
e-mail: info@diaclone.com
www.diaclone.com
Section 2 - Data on components
Chemical characterization: Preparation
The following data is for the 100% chemical as no data is available on the solution.
Hazardous ingredients: % CAS Number EC Number
3-Amino-9-ethylcarbazole 132-32-1 N/A
Section 3 - Hazard Identification
Hazard Description: harmful if swallowed, inhaled or absorbed through skin.
May cause skin irritation.
Section 4 - First Aid Measures
After skin contact: Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and
shoes.
After eye contact: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper
and lower lid.
After ingestion Wash out mouth with water provided and then drink 2-4 cupfuls of water, and seek medical advice.
Section 5 - Fire Fighting Measures
Suitable extinguishing agents
Use dry chemical, carbon dioxyde or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipement Use respirator and protective clothing. Toxic fumes could be emitted when water is
evaporated with fire.
Section 6 - Accidental Release Measures
Person-related safety precautions: Not required.
Measures for environmental protections:
Evacuate area and ventilate area.
Wear self-contained breathing apparatus, rubber boots and heavy rubber gloves. Absorb on sand or vermiculite and
place in closed containers for waste disposal.
Avoid splashing.
Section 7- Handling and storage
Refer to section 8.
Section 8 - Exposure Controls / Personal Protection
The usual precautionary measures are to be adhered to when handling chemicals.
Chemical safety goggles.
Compatible resistant gloves.
Avoid any skin or clothing contact.
Wash thoroughly after handling.
Keep tightly closed and refrigerate.
Section 9 - Physical and Chemical Properties
Form: Liquid
Colour: Clear deep reddish solution
Odour: Odourless
Approved by / Approuvé par
Issue N°1 July,23 2002 S.Thomas / J. Wijdenes
7
Material Safety Data Sheet
According to 91/155 EEC
Melting point/Melting range: 0°C (water)
Boiling point/Boiling range: 100°C (water)
Flash point: Not applicable
Autoignition temperature: N/A
Danger of explosion: Product does not present an explosion hazard
Densitiy: N/A
Solubility in / Miscibility with
water : fully miscible
Section 10 - Stability and Reactivity
Thermal decomposition: No decomposition if used according to specifications
Reactivity and under what conditions:
Incompatibles with strong oxidating agents and strong bases.
Hazardous decomposition products
Toxic fumes of carbon monoxyde, carbon dioxyde.
Section 11 - Toxicological Information
Route of entry: Skin Contact, Eye contact, Inhalation, Ingestion
Effects of acute exposure to Product:
Harmful if swallowed, inhaled or absorbed through skin. May cause irritation.
Effects of chronic exposure to Product:
Carcinogen.
Lab experiments have shown mutagenic effects.
To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly
investigated.
Effects of overexposure:
INHALATION: Adverse health effects from vapors and spray mists in poorly ventilated areas may include irritation of
the mucous membranes of the nose, throat, respiratory tract and symptoms of headache and nausea.
SKIN CONTACT: Avoid all contact. May cause cancer.
EYE CONTACT: Avoid all contact.
Section 12 - Ecological Information
General Notes: N/A
Section 13 - Disposal considerations
Waste disposal:
Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equiped with an after burner
and scrubber.
Observe all local official environmental regulations.
Section 14 - Transport Information
Special shipping information:
Does not need to be shipped as hazardous.
Section 15 - Regulations
Labelling according to European labelling guidelines:
Observe the general safety regulations when handling chemicals.
Section 16 - Other information
This information is prepared on our present knowledge. However, this shall not constitute a guarantee for any specific
product features and shall not establish a legally valid contractual relationship.
Prepared by: Marketing Department Phone N°:+33 3 81 41 38 38 Date: July 02
Approved by / Approuvé par
Issue N°1 July,23 2002 S.Thomas / J. Wijdenes
8
Material Safety Data Sheet
According to 91/155 EEC
Section 1 - Identification of substance
Product Identification: Dual Color Eli-spot PVDF Enzymatic kits
AEC Buffer
Manufacturer: DIACLONE SAS
1, Bd Fleming
F-25000 Besançon
France
Tel: +33 3 81 41 38 38
Fax: +33 3 81 41 36 36
e-mail: info@diaclone.com
www.diaclone.com
Section 2 - Data on components
Chemical characterization: Preparation
The following data is for the 100% chemical as no data is available on the solution.
Hazardous ingredients: % CAS Number EC Number
Soidum acetate 127-09-3 N/A
Section 3 - Hazard Identification
Hazard Description: harmful if swallowed, inhaled or absorbed through skin.
May cause skin irritation.
Section 4 - First Aid Measures
After skin contact: Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and
shoes.
After eye contact: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper
and lower lid.
After ingestion Wash out mouth with water provided and then drink 2-4 cupfuls of water, and seek medical advice.
Section 5 - Fire Fighting Measures
Suitable extinguishing agents
Use dry chemical, carbon dioxyde or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipement Use respirator and protective clothing. Toxic fumes could be emitted when water is
evaporated with fire.
Section 6 - Accidental Release Measures
Person-related safety precautions: Not required.
Measures for environmental protections:
Evacuate area and ventilate area.
Wear self-contained breathing apparatus, rubber boots and heavy rubber gloves. Absorb on sand or vermiculite and
place in closed containers for waste disposal.
Avoid splashing.
Section 7- Handling and storage
Refer to section 8.
Section 8 - Exposure Controls / Personal Protection
The usual precautionary measures are to be adhered to when handling chemicals.
Chemical safety goggles.
Compatible resistant gloves.
Avoid any skin or clothing contact.
Wash thoroughly after handling.
Keep tightly closed and refrigerate.
Section 9 - Physical and Chemical Properties
Form: Liquid
Colour: Clear solution
Odour: Odourless
Approved by / Approuvé par
Issue N°1 July,23 2002 S.Thomas / J. Wijdenes
9
Material Safety Data Sheet
According to 91/155 EEC
Melting point/Melting range: 0°C (water)
Boiling point/Boiling range: 100°C (water)
Flash point: Not applicable
Autoignition temperature: N/A
Danger of explosion: Product does not present an explosion hazard
Densitiy: N/A
Solubility in / Miscibility with
water : fully miscible
Section 10 - Stability and Reactivity
Thermal decomposition: No decomposition if used according to specifications
Reactivity and under what conditions:
Incompatibles with strong reducing agents and acides.
Hazardous decomposition products
Toxic fumes of carbon monoxyde, carbon dioxyde.
Section 11 - Toxicological Information
Route of entry: Skin Contact, Eye contact, Inhalation, Ingestion
Effects of acute exposure to Product:
Harmful if swallowed, inhaled or absorbed through skin. May cause irritation.
Effects of overexposure:
INHALATION: Adverse health effects from vapors and spray mists in poorly ventilated areas may include irritation of
the mucous membranes of the nose, throat, respiratory tract and symptoms of headache and nausea.
SKIN CONTACT: Avoid all contact. May cause cancer.
EYE CONTACT: Avoid all contact.
Section 12 - Ecological Information
General Notes: N/A
Section 13 - Disposal considerations
Waste disposal:
Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equiped with an after burner
and scrubber.
Observe all local official environmental regulations.
Section 14 - Transport Information
Special shipping information:
Does not need to be shipped as hazardous.
Section 15 - Regulations
Labelling according to European labelling guidelines:
Observe the general safety regulations when handling chemicals.
Section 16 - Other information
This information is prepared on our present knowledge. However, this shall not constitute a guarantee for any specific
product features and shall not establish a legally valid contractual relationship.
Prepared by: Marketing Department Phone N°:+33 3 81 41 38 38 Date: July 02
Approved by / Approuvé par
Issue N°1 July,23 2002 S.Thomas / J. Wijdenes
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