Material Safety Data Sheet

                                        acc. to OSHA and ANSI

    Printing date 03/06/2001                                           Reviewed on 05/05/2001





�        1 Identification of substance:



     �    Product details:





     �    Trade name: Tris(hydroxymethyl)aminomethane





     �    Part number: 11740





     �    Manufacturer/Supplier:

          ELECTRON MICROSCOPY SCIENCES, 321 MORRIS ROAD, FORT WASHINGTON, PA 19034





     �    CHEMTREC: (800) 424-9300





     �

          Web Site: www.emsdiasum.com



     �    Information department: Health, Safety and Environmental Department



     �    Emergency information:

          During normal hours the Health, Safety and Environmental Department. After normal

          hours call Chemtrec at (800) 424-9300.



�        2 Composition/Data on components:



     �    Chemical characterization:

                     Description: (CAS#)

                      Tris(hydroxymethyl)aminomethane (CAS# 77-86-1); 100%



     �    Identification number(s):



     �    EINECS Number: 201-064-4



�        3 Hazards identification



     �    Hazard description: Xi Irritant



     �    Information pertaining to particular dangers for man and environment

          R 36/37/38 Irritating to eyes, respiratory system and skin.



�        4 First aid measures



     �    After inhalation

          Supply fresh air. If required, provide artificial respiration. Keep patient warm.

          Seek immediate medical advice.



     �    After skin contact

          Immediately wash with water and soap and rinse thoroughly.

          Seek immediate medical advice.

         Seek immediate medical advice.



    �    After eye contact

         Rinse opened eye for several minutes under running water. Then consult a doctor.



    �    After swallowing Seek immediate medical advice.



�       5 Fire fighting measures



    �    Suitable extinguishing agents

         Carbon dioxide, extinguishing powder or water spray. Fight larger fires with water

         spray or alcohol resistant foam.



    �    Protective equipment:

         Wear self-contained respirator.

         Wear fully protective impervious suit.



�       6 Accidental release measures



    �    Person-related safety precautions:

         Wear protective equipment. Keep unprotected persons away.

         Ensure adequate ventilation



    �    Measures for environmental protection:

         Do not allow material to be released to the environment without proper governmental

         permits.



    �    Measures for cleaning/collecting: Ensure adequate ventilation.



    �    Additional information:

         See Section 7 for information on safe handling

         See Section 8 for information on personal protection equipment.

         See Section 13 for disposal information.



�       7 Handling and storage







    �    Handling



    �    Information for safe handling:

         Keep container tightly sealed.

         Store in cool, dry place in tightly closed containers.

         Ensure good ventilation at the workplace.



    �    Information about protection against explosions and fires:

         No special measures required.





    �    Storage



    �    Requirements to be met by storerooms and receptacles:

         No special requirements.



    �    Information about storage in one common storage facility:

         Store away from oxidizing agents.

         Store away from water/moisture.



    �    Further information about storage conditions:

         Keep container tightly sealed.

         Store in cool, dry conditions in well sealed containers.

         Store in cool, dry conditions in well sealed containers.



�       8 Exposure controls and personal protection



    �    Additional information about design of technical systems:

         Properly operating chemical fume hood designed for hazardous chemicals and having

         an average face velocity of at least 100 feet per minute.



         Components with limit values that require monitoring at the workplace:

         Not required.



    �    Additional information: No data





    �    Personal protective equipment



    �    General protective and hygienic measures

         The usual precautionary measures for handling chemicals should be followed.

         Keep away from foodstuffs, beverages and feed.

         Remove all soiled and contaminated clothing immediately.

         Wash hands before breaks and at the end of work.

         Avoid contact with the eyes and skin.



    �    Breathing equipment:

         Use suitable respirator when high concentrations are present.



    �    Protection of hands: Impervious gloves



    �    Eye protection:

         Safety glasses

         Tightly sealed goggles

         Full face protection



    �    Body protection: Protective work clothing.



�       9 Physical and chemical properties:







    �    Form: Solid



    �    Color: White



    �    Odor: Not determined



    �                                           Value/Range    Unit   Method



    �    Change in condition



    �    Melting point/Melting range:                 171-172 ° C



    �    Boiling point/Boiling range:         Not determined



    �    Sublimation temperature / start:     Not determined





    �    Flash point:                         Not applicable





    �    Ignition temperature:                Not determined

    �   Decomposition temperature:         Not determined





    �   Danger of explosion:

        Product does not present an explosion hazard.



    �   Explosion limits:



    �   Lower:                             Not determined



    �   Upper:                             Not determined





    �   Vapor pressure:                    Not determined





    �   Density:                           Not determined





    �   Solubility in / Miscibility with



    �   Water:                             Soluble



�   10 Stability and reactivity



    �   Thermal decomposition / conditions to be avoided:

        Decomposition will not occur if used and stored according to specifications.



    �   Materials to be avoided:

        Water/moisture

        Oxidizing agents



    �   Dangerous reactions No dangerous reactions known



    �   Dangerous products of decomposition:

        Carbon monoxide and carbon dioxide

        Nitrogen oxides



�   11 Toxicological information



    �   Acute toxicity:

        LD/Lc50 values that are relevant for classification:

        Oral: LD50: 5900 mg/kg (rat)

        LD50: 1000 mg/kg (rbt)



    �   Primary irritant effect:



    �   on the skin: Irritant to skin and mucous membranes.



    �   on the eye: Irritating effect.



    �   Sensitization: No sensitizing effects known.



    �   Subacute to chronic toxicity:

        Other than potential irritation (see above), no information on illness or injury

        from acute or chronic exposure to this product is available.



    �   Additional toxicological information:

        To the best of our knowledge the acute and chronic toxicity of this substance is

        not fully known.

        No classification data on carcinogenic properties of this material is available

        from the EPA, IARC, NTP, OSHA or ACGIH.

        not fully known.

        No classification data on carcinogenic properties of this material is available

        from the EPA, IARC, NTP, OSHA or ACGIH.



�   12 Ecological information:



    �   General notes:

        Do not allow material to be released to the environment without proper governmental

        permits.



�   13 Disposal considerations







    �   Product:



    �   Recommendation

        Consult state, local or national regulations for proper disposal.



    �   Uncleaned packagings:



    �   Recommendation:

        Disposal must be made according to official regulations.



    �   Recommended cleansing agent: Water, if necessary with cleansing agents.



�   14 Transport information



        Not a hazardous material for transportation.



    �   DOT regulations:



    �   Hazard class:                None





    �   Land transport ADR/RID     (cross-border)



    �   ADR/RID class:                      None





    �   Maritime transport IMDG:



    �   IMDG Class:                  None





    �   Air transport ICAO-TI and IATA-DGR:



    �   ICAO/IATA Class:             None





    �   Transport/Additional information:

        Not dangerous according to the above specifications.



�   15 Regulations







    �   Product related hazard informations:

    �   Hazard symbols: Xi Irritant





    �   Risk phrases:

        36/37/38 Irritating to eyes, respiratory system and skin.



    �   Safety phrases:

        26       In case of contact with eyes, rinse immediately with plenty of water and

        seek medical advice.





    �   National regulations

        All components of this product are listed in the U.S. Environmental Protection

        Agency Toxic Substances Control Act Chemical Substance Inventory.





    �   Information about limitation of use:

        For use only by technically qualified individuals.



�   16 Other information:



        Employers should use this information only as a supplement to other information

        gathered by them, and should make independent judgement of suitability of this

        information to ensure proper use and protect the health and safety of employees.

         This information is furnished without warranty, and any use of the product not in

        conformance with this Material Safety Data Sheet, or in combination with any other

        product or process, is the responsibility of the user.