PROMETIC
BIOSCIENCES
Perfluorosorb S 庐
Remove endotoxin
Remove proteins
Unique synthetic adsorbent for
Remove RNA and
the purification of plasmid DNA
genomic DNA
Gene therapy and DNA vaccination are rapidly evolving practices as
Reuse with documented
innovation in biotechnology and an industry鈥檚 dedication to science
CIP procedure continues to seek cures for genetic disorders and infectious disease.
Run at flow rates up ProMetic BioSciences is proud to play a role in the advancement of life
science. With the introduction of Perfluorosorb庐 S, we have applied our
to 700cm/hr with low
core capabilities in the design and manufacture of synthetic, particulate
back-pressures chromatographic media to this important field.
Drug Master File Perfluorosorb S is a unique, patented product for the purification of
plasmid DNA. It has been specifically engineered to preferentially
registered with FDA
adsorb super-coiled DNA from mixtures of RNA, endotoxin, proteins
and other isoforms of DNA and thus provides DNA of the highest
Manufactured in purity and potency for laboratory experimentation, clinical trial, and
GMP facility therapeutic manufacture.
www.prometic.com
Perfluorosorb S 庐
Since 1987 ProMetic has been We collaborate with pharmaceutical
pioneering design, development, and biotech companies to optimize
and manufacture of affinity bioseparation and purification
purification technology for large- processes. Our patented separation
scale bioprocessing as well as technology and expansive Chemical
research and development at Combinatorial Library鈩? of synthetic
lab-scale in the biotechnology ligand media increases production
industry. Our patented Mimetic efficiency, reduces manufacturing
Ligand鈩? technology synthetically costs and enables companies to
鈥渕imics鈥? and enhances the natural strengthen market positions through
molecular affinity of binding ligands continuous improvements in product
towards targeted biomolecules. purity and yield.
The result: Optimized binding and Introducing Perfluorosorb庐 S
yields for all your biomolecular Perfluorosorb S is an engineered,
purification needs.The key to our polymeric adsorbent for the purifi-
We develop and produce a variety of affinity success in biomolecule purification cation of plasmid DNA using
purification media for the concentration
lies in the development of an reverse phase chromatography.
and separation of therapeutic antibodies,
extensive Chemical Combinatorial It鈥檚 highly porous structure, broad
plasma proteins, plasmid DNA, transgenic
Library鈩?. Through years of
therapeutics, enzymes, vaccines and more. pH resistance and high binding
dedication, research and develop- capacity make it ideal for purifica-
ment we鈥檝e systematically screened tion of supercoiled pDNA.
countless ligand arrays and utilized Perfluorosorb S is conveniently
Primary capture our computer modeling processes available as a slurry in 24% Ethanol
and polishing to design new ligand structures. in water for improved results in a
number of purification processes:
Dedicated to ISO9001
High and low
quality and increased 鈥? Primary capture, post-cell lysis
copy plasmids and clarification
production efficiency
鈥? As a polishing agent after
F
Quality and innovation go hand in
Large and small ion exchange to remove:
hand. That鈥檚 why we work hard to
- endotoxin
DNA molecules
F
maintain and even exceed ISO9001
- genomic DNA
standards. By staying close to our
- occ and other
customers and their unique
Useful with endotoxin
F
DNA isoforms
requirements we enhance our
concentrations above - protein
F
ability to provide innovative solutions
- RNA
5000 EU/mg DNA to meet the needs of the bio-
F
pharmaceutical market.
F F
One such innovation is our
Milligram and PuraBead庐, cross-linked, agarose
F
multigram quantities matrix designed for high flow and
of pDNA maximum binding capacity.
F F
F
F
F
F
F
www.prometic.com
F
PROMETIC
BIOSCIENCES
Perfluorosorb S 庐
Perfluorosorb at work Bioavailability
Plasmid DNA binds to Perfluorosorb The transfection efficiency of
in neutral buffers containing ion- plasmid DNA purified using
pairing agents. Endotoxins are Perfluorosorb S is at least as good
mainly removed in the flow-through as pDNA obtained using other
and residual amounts are cleared methods. For example, CCL39
along with RNA in a wash step. fibroblasts that were transiently
transfected with a plasmid encoding
The super-coiled form of plasmid for Green Fluorescent Protein
DNA preferentially binds to showed that transfection efficiencies
Perfluorosorb using reverse phase of more than 60% could be Photomicrograph of CCL 39 Fibroblasts
transiently transfected with a green
buffers. Ion-pairing agents are achieved using plasmids isolated
fluorescent protein-containing plasmid
used to enhance separations and using Perfluorosorb S. purified using Perfluorosorb S.
resolution in reverse phase
chromatography and are totally The proof is in the purity
removed by the wash buffers. As an example of the exceptional
ability of Perfluorosorb S to perform
The addition of electrolytes, which at process or laboratory scale,
are usually present after an ion- following is a typical purity profile
change step, will actually positively of polished DNA that can
affect the binding between the be achieved:
extremely hydrophobic surface
>95% supercoiled DNA
and exposed polar surface of the
1.8
separation target.
<2% genomic DNA
<3 EU endotoxin/mg pDNA
<2% protein
Fluorinated surface
Proper restriction pattern
Co-migrates with reference
鈥?
RNA <2%
+ 鈥?
F Plasmid DNA
鈥?
F Hydrophobic tail
鈥?
F 鈥?
鈥?
鈥? Cation end
F
F +
鈥?
F Electrophoresis of DNA produced for an
F Avian West Nile Virus vaccine when
鈥?
+鈥? Perfluorosorb was used as the polishing
F step to remove endotoxin and to enrich the
supercoiled pVAX-WN. (courtesy of
Aldevron LLC, 2003).
F
F
F Ion pairing agent
鈥?
鈥?
+
F 鈥?
F www.prometic.com
鈥?
鈥?
F +
Perfluorosorb S 庐
Get started on the road PRODUCT SPECIFICATIONS
to better results New synthetic, fluorinated polymeric entity
No special equipment is needed to
Spherical, porous particles
get started on plasmid purification
Mean particle size: 45碌m
with Perfluorosorb. Simply pack
into a standard column using Pure pDNA binding capacity: up to 0.7mg/mL
10碌m frits. Follow the supplied
Flow rates: up to 700 cm/hr at 1 bar (15psi)
packing instructions and special
Stable in pH range 1-14
buffer protocols. Perfluorosorb
columns can be run on any low Resistant to 5M sodium hydroxide
pressure chromatography work-
Suited to repeat-use bioprocess applications
station. In fact, Perfluorosorb is
Store at 2-30掳C in 24% ethanol
strong enough for use in an HPLC
environment, if desired. If you
intend to use Perfluorosorb in pro-
duction of cGMP plasmid DNA,
ask for ProMetic鈥檚 Drug Master File
reference. A Material Safety Data
Sheet (MSDS) is also available.
ORDERING INFORMATION
International Sales and
Technical Support: Perfluorosorb庐 S (suspension for packing into columns)
ProMetic BioSciences (USA) Inc.
25 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5003-00025
155 Willowbrook Boulevard,
100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5003-00100
Suite 460
Wayne, NJ 07470, USA 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5003-00500
Tel: +1.973.812.9880 1000 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5003-01000
Fax: +1.973.812.9881
(Please enquire for quantities greater than 1 liter.)
E-mail: sales@prometic.com
techsupport@prometic.com
www.prometic.com
PROMETIC
BIOSCIENCES 漏 ProMetic BioSciences, Inc. 2004
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