铮?
Product Information Sheet for ATCC庐 52-X
NO RETURN, REPLACEMENT, OR CREDIT WILL BE
POLYETHYLENE GLYCOL 4000 (PEG-4000)
AUTHORIZED AFTER 30 DAYS FROM RECEIPT OF
Molecular Weight Range 3000-3700
PRODUCT(S).
CAS# 25322-68-3
Disclaimer of Warranties
Product Description: There are no warranties, express or implied, including,
This reagent is used to assist fusion of myeloma and without limitation, any implied warranty of merchantability or
spleen cells to form hybridomas. PEG-4000 is packaged as 2 fitness for a particular purpose. ATCC is not liable for any
gram aliquots in amber vials that protect the product from damages or injuries resulting from receipt and/or improper,
light. PEG is undiluted and is a white solid. inappropriate, unintended wrongful or negligent handling or
use of an ATCC product. Buyers' exclusive remedy for any
losses resulting from defective or nonconforming goods,
NOT FOR HUMAN ADMINISTRATION -- NOT
breach of warranty or any other cause, including negligence or
FOR DIAGNOSTIC PURPOSES.
strict liability, shall be suitable replacement of goods.
FOR LABORATORY RESEARCH ONLY
EXCLUSIVE REMEDY: The exclusive remedy against
To Use:
ATCC (including any contributor) for any losses or damage of
Melt solid PEG in oven or water bath or on a hot plate.
any kind whatsoever, whether in contract, tort or otherwise,
Cool molten PEG to about 50掳C and mix with 3.0 ml of the
shall be, at ATCC's option, refund of the fee paid to ATCC for
prewarmed sterile medium of your choice (RPMI, DMEM,
the affected material or other item, replacement of the
etc.). This results in a 40% w/v mixture. Cool to about 37掳C.
material, and/or repeat of the above-specified tests on the
PEG is now ready to use. The concentration can be varied by
material provided herewith.
the addition of more or less sterile medium.
漏 ATCC, 1999
Storage:
ATCC庐 is a registered trademark of the American Type
At the ATCC, PEG-4000 is stored at 2 to 8掳C, but the
Culture Collection
reagent may be stored at room temperature.
2/99 JAR
Quality Control:
MSDS FOR POLYETHYLENE
Each lot is tested for bacterial and fungal
GLYCOL
contamination. Cytotoxicity testing is performed on NS-1 cells
at a 40% w/v concentration. Cells must remain greater than
80% viable after exposure to PEG. For Information call: 703-365-2704
ATCC Emergency Telephone No.: 703-365-2710 (24 hours)
Replacement Policy: CHEMTREC: 800-424-9300 To be used only in the event
Notify ATCC immediately if there are any problems of an Emergency involving a spill, leak, fire, exposure or
with your order. Please call our Customer Service Department accident.
at 1-800-638-6597 or fax at 1-703-365-2750. Unsatisfactory
1. Product Identification
products due to errors by ATCC will be replaced free of
CAS No.: 25322-68-3
charge if reported within 30 days of receipt of the product(s).
Molecular Weight: Not applicable to mixtures.
Chemical Formula: (C2H4O) n.H2O
Return Policy:
Authorization, including a "Return Authorization 2. Composition/Information on Ingredients
Number" and shipping instructions must be obtained from the Ingredient CAS No Percent Hazardous
ATCC Sales Department prior to returning any item(s). Some Polyethylene Glycol 25322-68-3 90 - 100% No
Products may not be returnable. The following information is
3. Hazards Identification
required:
Emergency Overview
-Reason for return
As part of good industrial and personal hygiene and safety
-Purchase Order Number
procedure, avoid all unnecessary exposure to the chemical
-Sales Order Number substance and ensure prompt removal from skin, eyes and
-Product Description & Catalog number clothing.
-Batch/lot number Potential Health Effects:
-Quantity Inhalation: No adverse health effects expected from inhalation.
-Date Received (May be a mechanical irritant.)
Ingestion: Large doses of the lower molecular weight products
NOTE: Returns will not be accepted without a Return
may cause gastro-intestinal upset.
Authorization Number.
American Type Culture Collection Ordering info: 800-638-6597 (US and Canada)
P.O. Box 1549 703-365-2700 (elsewhere); Fax: 703-365-2750
Manassas, VA 20108 E-mail: sales@atcc.org; Web site: www.atcc.org
-1-
铮?
Product Information Sheet for ATCC庐 52-X
Skin Contact: No adverse effects expected. For use with solids (not required for liquids): If the exposure
Eye Contact: No adverse effects expected. limit is exceeded, a half-face dust/mist respirator may be worn
Chronic Exposure: No information found. for up to ten times the exposure limit or the maximum use
Aggravation of Pre-existing Conditions: Damaged skin. concentration specified by the appropriate regulatory agency or
respirator supplier, whichever is lowest. A full-face piece
dust/mist respirator may be worn up to 50 times the exposure
4. First Aid Measures
Inhalation: Not expected to require first aid measures. limit, or the maximum use concentration specified by the
Ingestion: If large amounts were swallowed, give water to drink appropriate regulatory agency, or respirator supplier, whichever
and get medical advice. is lowest. For emergencies or instances where the exposure
Skin Contact: In case of contact, immediately flush skin with levels are not known, use a full-facepiece positive-pressure, air-
plenty of soap and water for at least 15 minutes. Remove supplied respirator. WARNING: Air-purifying respirators do not
contaminated clothing and shoes. Wash clothing before reuse. protect workers in oxygen-deficient atmospheres.
Get medical attention if irritation develops or persists. Skin Protection: Wear protective gloves and clean body-
Eye Contact: In case of contact, flush eyes with plenty of water covering clothing.
for at least 15 minutes. Get medical advice if irritation develops. Eye Protection: Use chemical safety goggles. Maintain eye
wash fountain and quick-drench facilities in work area.
5. Fire Fighting Measures
Fire: As with most organic solids, fire is possible at elevated 9. Physical and Chemical Properties
temperatures or by contact with an ignition source(increases as Appearance: Clear liquid or white solid.
molecular weight increases). Flash point: 182 - 287 掳C. Odor: Mild odor.
Explosion: Fine dust dispersed in air in sufficient Solubility: Soluble in water.
concentrations, and in the presence of an ignition source is a Density: range: 1.1 to 1.2 (increases as molecular weight
potential dust explosion hazard. increases)
Fire Extinguishing Media: Water spray, dry chemical, alcohol pH: No information found.
foam, or carbon dioxide. % Volatiles by volume @ 21掳C (70掳F): No information found.
Special Information: In the event of a fire, wear full protective Boiling Point: No information found.
clothing and NIOSH-approved self-contained breathing Melting Point: Melting point increases as molecular weight
apparatus with full face-piece operated in the pressure demand increases: PEG 400 = 4-8掳C (39-46掳F); PEG 600 = 20-25掳C
or other positive pressure mode. (68-77掳F); PEG1500 = 44-48掳C (111-118掳F); PEG 4000 = 54-
58掳C (129-136掳F); PEG 6000 = 56-63掳C (133-145掳F).
Vapor Density (Air=1): No information found.
6. Accidental Release Measures
Remove all sources of ignition. Ventilate area of leak or spill. Vapor Pressure (mm Hg): Vapor pressure is very low; as
Wear appropriate personal protective equipment as specified in molecular weight increases, vapor pressure decreases.
Section 8. Solid Spills: Clean up spills in a manner that does not Evaporation Rate (BuAc=1): No information found.
disperse dust into the air. Use non-sparking tools and equipment.
Reduce airborne dust and prevent scattering by moistening with 10. Stability and Reactivity
water. Pick up spill for recovery or disposal and place in a Stability: Stable under ordinary conditions of use and storage.
closed container. Liquid Spills: Absorb with vermiculite, dry Hazardous Decomposition Products: Carbon dioxide and
sand, earth or similar material and place in a chemical waste carbon monoxide may form when heated to decomposition.
container. Do not use combustible materials, such as sawdust. Hazardous Polymerization: Will not occur.
Do not flush to sewer. Incompatibilities: Incompatible with polymerization catalysts
(peroxides, persulfates) and accelerators, strong oxidizers,
strong bases and strong acids.
7. Handling and Storage
Keep in a tightly closed container, stored in a cool, dry, Conditions to Avoid: Incompatibles.
ventilated area. Protect against physical damage. Containers of
this material may be hazardous when empty since they retain 11. Toxicological Information
product residues (dust, solids, vapors, liquid); observe all Oral Rat LD50 for: PEG 200 = 28gm/kg; PEG 300 =
warnings and precautions listed for the product. 27.5gm/kg; PEG 400 = 30.2gm/kg; PEG 600 = 30gm/kg; PEG
1000 = 32gm/kg; PEG 1450 = >4gm/kg; PEG 4000 = 50gm/kg;
PEG 6000 = > 50gm/kg; PEG 20000 = 31.6gm/kg Polyethylene
8. Exposure Controls/Personal Protection
Airborne Exposure Limits: AIHA Workplace Environmental glycol has been investigated as a mutagen. PEG 1000 has been
Exposure Level (WEEL): 10 mg/m3, 8-hour, TWA investigated as a tumorigen.
Ventilation System:
A system of local and/or general exhaust is recommended to Cancer Lists
keep employee exposures below the Airborne Exposure Limits. ---NTP Carcinogen---
Local exhaust ventilation is generally preferred because it can Ingredient Known Anticipated IARC Category
control the emissions of the contaminant at its source, PEG (25322-68-3) No No None
preventing dispersion of it into the general work area. Please
refer to the ACGIH document, Industrial Ventilation, A Manual 12. Ecological Information
of Recommended Practices, most recent edition, for details. Environmental Fate: No information found.
Environmental Toxicity: No information found.
Personal Respirators (NIOSH Approved):
American Type Culture Collection Ordering info: 800-638-6597 (US and Canada)
P.O. Box 1549 703-365-2700 (elsewhere); Fax: 703-365-2750
Manassas, VA 20108 E-mail: sales@atcc.org; Web site: www.atcc.org
-2-
铮?
Product Information Sheet for ATCC庐 52-X
must be used to avoid contact with material or breathing chemical
13. Disposal Considerations
Whatever cannot be saved for recovery or recycling should be vapors/fumes.
managed in an appropriate and approved waste disposal facility.
Processing, use or contamination of this product may change the
waste management options. State and local disposal regulations
may differ from federal disposal regulations. Dispose of
container and unused contents in accordance with federal, state
and local requirements.
14. Transport Information
Not regulated.
15. Regulatory Information
Chemical Inventory Status - Part 1:
Ingredient TSCA EC Japan Australia
PEG (25322-68-3) Yes No Yes Yes
Chemical Inventory Status - Part 2:
----Canada----
Ingredient Korea DSL NDSL Phil.
PEG (25322-68-3) Yes Yes No Yes
Federal, State & International Regulations - Part 1:
-SARA 302- ------SARA 313------
Ingredient RQ TPQ List Chemical Catg.
PEG (25322-68-3) No No No No
Federal, State & International Regulations - Part 2:
-RCRA- -TSCA-
Ingredient CERCLA 261.33 8(d)
PEG(25322-68-3) No No No
Chemical Weapons Convention: No TSCA 12(b): No
CDTA: No
SARA 311/312: Acute: No; Chronic: No; Fire: No;
Pressure: No Reactivity: No; (Pure / Solid)
Australian Hazchem Code: No information found.
Poison Schedule: No information found.
WHMIS: This MSDS has been prepared according to the
hazard criteria of the Controlled Products Regulations (CPR)
and the MSDS contains all of the information required by the
CPR.
16. Other Information
NFPA Ratings: Health: 0 Flammability: 1 Reactivity: 0
Label Hazard Warning: As part of good industrial and
personal hygiene and safety procedure, avoid all unnecessary
exposure to the chemical substance and ensure prompt removal
from skin, eyes and clothing.
Label Precautions: None.
Label First Aid: Not applicable.
Product Use: Laboratory Reagent.
This document is intended only as a guide to the appropriate
precautionary handling of the material by a person trained in, or
supervised by a person trained in, chemical handling. The user is
responsible for determining the precautions and dangers of this
chemical for his or her particular application. Depending on usage,
protective clothing including eye and face guards and respirators
American Type Culture Collection Ordering info: 800-638-6597 (US and Canada)
P.O. Box 1549 703-365-2700 (elsewhere); Fax: 703-365-2750
Manassas, VA 20108 E-mail: sales@atcc.org; Web site: www.atcc.org
-3-
|