铮?
MATERIAL SAFETY DATA SHEET
MSDS FOR ATCC MICROBIAL CULTURES (Biosafety Level 1)
ATCC cultures are not hazardous as defined by OSHA 1910.1200. However, as living
microorganisms they are potential biohazards.
ATCC Emergency Telephone: (703) 365-2710 (24 hours)
Chemtrec: (800) 424-9300
To be used only in the event of an emergency involving a spill, leak, fire, exposure or accident.
Description
ATCC microbial cultures consist of all bacteria, fungi, plant and animal viruses, and molecular
biology materials such as hosts, vectors, clones and libraries.
Either frozen, freeze-dried or growing cells shipped on solid or liquid culture medium (a mixture of
components that may include, but is not limited to: inorganic salts, vitamins, amino acids,
carbohydrates and other nutrients dissolved in water).
SECTION I
Hazardous Ingredients
Frozen cultures may contain 5 to 10% Dimethyl sulfoxide (DMSO).
SECTION II
Physical data
Liquid or solid suspensions; frozen liquid suspensions; freeze-dried.
SECTION III
Health hazards
This culture is not known to cause disease in healthy human adults or animals.
SECTION IV
Fire and explosion
Not applicable
SECTION V
Reactivity data
Stable. Hazardous polymerization will not occur.
American Type Culture Collection Emergency Telephone: (703) 365-2710 (24 hours)
P.O. Box 1549 Information Telephone: (703) 365-2704
Manassas, VA 20108 1 Chemtrec (800) 424-9300
铮?
MATERIAL SAFETY DATA SHEET
SECTION VI
Method of disposal
Spill: Contain the spill and decontaminate using suitable disinfectants such as chlorine bleach or
70% ethyl or isopropyl alcohol.
Waste disposal: Dispose of cultures and exposed materials by autoclaving at 121掳C for 20 minutes.
Dispose of sealed vials of freeze-dried material by dry heat sterilization at 170掳C
for four hours.
Follow all Federal, State and local regulations.
SECTION VII
Special protection information
For Biosafety Level 1 Microbial Cultures
Handle as a potentially biohazardous material under at least Biosafety Level 1 containment.
SECTION VIII
Special precautions or comments
ATCC recommends that all ATCC microbial cultures be handled by qualified microbiologists
using appropriate safety procedures and precautions. Detailed discussions of laboratory safety
procedures are provided in Laboratory Safety: Principles and Practice (Fleming et al., ASM
Press, Washington, DC, 1995), and in the U.S. Government Publication, Biosafety in
Microbiological and Biomedical Laboratories (CDC, 1999). This publication is available in its
entirety in the Center for Disease Control Office of Health and Safety鈥檚 web site at
http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm.
Information on the classification of human etiologic agents on the basis of hazard can be found as
Appendix B in the NIH Guidelines for Research Involving Recombinant DNA Molecules at
http://grants.nih.gov/grants/policy/recombinentdnaguidelines.htm.
THE ABOVE INFORMATION IS CORRECT TO THE BEST OF OUR KNOWLEDGE. ALL
MATERIALS AND MIXTURES MAY PRESENT UNKNOWN HAZARDS AND SHOULD BE
USED WITH CAUTION. THE USER SHOULD MAKE INDEPENDENT DECISIONS
REGARDING THE COMPLETENESS OF THE INFORMATION BASED ON ALL SOURCES
AVAILABLE. ATCC SHALL NOT BE HELD LIABLE FOR ANY DAMAGE RESULTING
FROM HANDLING OR CONTACT WITH THE ABOVE PRODUCT.
铮? 2002 American Type Culture Collection.
ATCC铮? is a registered trademark of the American Type Culture Collection.
February 2002
American Type Culture Collection Emergency Telephone: (703) 365-2710 (24 hours)
P.O. Box 1549 Information Telephone: (703) 365-2704
Manassas, VA 20108 2 Chemtrec (800) 424-9300
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