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                                                    MATERIAL SAFETY DATA SHEET





           MSDS FOR ANIMAL CELL CULTURES (Biosafety Level 1 or 2)



ATCC cultures are not hazardous as defined by OSHA 1910.1200. However, as live cells they

are potential biohazards.



ATCC Emergency Telephone: (703) 365-2710 (24 hours)

Chemtrec: (800) 424-9300

To be used only in the event of an emergency involving a spill, leak, fire, exposure or accident.



Description

   Either frozen or growing cells shipped in liquid cell culture medium (a mixture of components that

   may include, but is not limited to: inorganic salts, vitamins, amino acids, carbohydrates and other

   nutrients dissolved in water).



SECTION I

Hazardous Ingredients

   Frozen cultures may contain 5 to 10% Dimethyl sulfoxide (DMSO)



SECTION II

Physical data

   Pink or red aqueous liquid



SECTION III

Health hazards

   For Biosafety Level 1 Cell Lines

   This cell line is not known to harbor an agent known to cause disease in healthy adult humans. This

   cell line has NOT been screened for Hepatitis B, human immunodeficiency viruses or other

   adventitious agents. Handle as a potentially biohazardous material under at least Biosafety Level 1

   containment.



   For Biosafety Level 2 Cell Lines

   This cell line is known to contain an agent that requires handling at Biosafety Level 2 containment

   [U.S. Government Publication Biosafety in Microbiological and Biomedical Laboratories

   (CDC, 1999)]. These agents have been associated with human disease. This cell line has NOT

   been screened for Hepatitis B, human immunodeficiency viruses or other adventitious agents. Cell

   lines derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030

   Bloodborne Pathogens.



SECTION IV

Fire and explosion

   Not applicable









American Type Culture Collection                              Emergency Telephone: (703) 365-2710 (24 hours)

P.O. Box 1549                                                          Information Telephone: (703) 365-2704

Manassas, VA 20108                              1                                   Chemtrec (800) 424-9300

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                                                          MATERIAL SAFETY DATA SHEET



SECTION V

Reactivity data

   Stable. Hazardous polymerization will not occur.



SECTION VI

Method of disposal

  Spill: Contain the spill and decontaminate using suitable disinfectants such as chlorine bleach or

  70% ethyl or isopropyl alcohol.

  Waste disposal: Dispose of cultures and exposed materials by autoclaving at 121°C for 20 minutes.

  Follow all Federal, State and local regulations.



SECTION VII

Special protection information

   For Biosafety Level 1 Cell Lines

   Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cell

   lines derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030

   Bloodborne Pathogens.



    For Biosafety Level 2 Cell Lines

    Handle as a potentially biohazardous material under at least Biosafety Level 2 containment. Cell

    lines derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030

    Bloodborne Pathogens.



SECTION VIII

Special precautions or comments

   ATCC recommends that appropriate safety procedures be used when handling all cell lines,

   especially those derived from human or other primate material. Detailed discussions of laboratory

   safety procedures are provided in Laboratory Safety: Principles and Practice (Fleming, et al.,

   1995) the ATCC manual on quality control (Hay, et al., 1992), the Journal of Tissue Culture

   Methods (Caputo, 1988), and in the U.S. Government Publication, Biosafety in Microbiological

   and Biomedical Laboratories (CDC, 1999). This publication is available in its entirety in the

   Center for Disease Control Office of Health and Safety’s web site at

   http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm.





THE ABOVE INFORMATION IS CORRECT TO THE BEST OF OUR KNOWLEDGE. ALL

MATERIALS AND MIXTURES MAY PRESENT UNKNOWN HAZARDS AND SHOULD BE

USED WITH CAUTION. THE USER SHOULD MAKE INDEPENDENT DECISIONS

REGARDING THE COMPLETENESS OF THE INFORMATION BASED ON ALL SOURCES

AVAILABLE. ATCC SHALL NOT BE HELD LIABLE FOR ANY DAMAGE RESULTING

FROM HANDLING OR CONTACT WITH THE ABOVE PRODUCT.



� 2002 American Type Culture Collection.

ATCC� is a registered trademark of the American Type Culture Collection.

February 2002





American Type Culture Collection                                     Emergency Telephone: (703) 365-2710 (24 hours)

P.O. Box 1549                                                                 Information Telephone: (703) 365-2704

Manassas, VA 20108                                    2                                    Chemtrec (800) 424-9300