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Product Information Sheet for ATCC庐 69-X
-Product Description & Catalog number
HYPOXANTHINE-AMINOPTERIN-
-Batch/lot number
THYMIDINE
-Quantity
500X Concentrate -Date Received
ATCC庐 Catalog No. 69-X NOTE: Returns will not be accepted without a Return
Authorization Number.
NO RETURN, REPLACEMENT, OR CREDIT WILL BE
Product Description: AUTHORIZED AFTER 30 DAYS FROM RECEIPT OF
This reagent is used in hybridoma production to select PRODUCT(S).
hybridomas from normal cell populations. HAT is supplied as
five 2.0 ml vials. Each vial of concentrate can produce one Disclaimer of Warranties
liter of 1X HAT medium. There are no warranties, express or implied, including,
Each vial contains sterile, freeze-dried concentrate of: without limitation, any implied warranty of merchantability or
Hypoxanthine 13.610 mg fitness for a particular purpose. ATCC is not liable for any
Aminopterin 0.176 mg damages or injuries resulting from receipt and/or improper,
Thymidine 3.780 mg inappropriate, unintended wrongful or negligent handling or
BSA fraction V 20.000 mg use of an ATCC product. Buyers' exclusive remedy for any
losses resulting from defective or nonconforming goods,
NOT FOR HUMAN ADMINISTRATION -- NOT breach of warranty or any other cause, including negligence or
FOR DIAGNOSTIC PURPOSES. strict liability, shall be suitable replacement of goods.
FOR LABORATORY RESEARCH ONLY
EXCLUSIVE REMEDY: The exclusive remedy against
To Use: ATCC (including any contributor) for any losses or damage of
Rehydrate with sterile tissue culture medium to 1000 any kind whatsoever, whether in contract, tort or otherwise,
ml. shall be, at ATCC's option, refund of the fee paid to ATCC for
Final concentration: the affected material or other item, replacement of the
Hypoxanthine 0.100 mM material, and/or repeat of the above-specified tests on the
Aminopterin 0.400 碌M material provided herewith.
Thymidine 0.016 mM
漏 ATCC, 1999
Storage:
ATCC庐 is a registered trademark of the American Type
Storage temperature of 2-8掳C is recommended.
Culture Collection
3/99
Quality Control:
The P-3 and SP 2/0 cell lines were seeded in growth
medium. Cell concentration in cultures without HAT
MSDS FOR HYPOXANTHINE-
increased >10 fold in 4 days. Cultures with the addition of 1X
HAT were non-viable after 7 days. AMINOPTERIN-THYMIDINE
Replacement Policy: For Information call: 703-365-2704
Notify ATCC immediately if there are any problems ATCC Emergency Telephone No.: 703-365-2710 (24 hours)
with your order. Please call our Customer Service Department CHEMTREC: 800-424-9300 To be used only in the event
at 1-800-638-6597 or fax at 1-703-365-2750. Unsatisfactory of an Emergency involving a spill, leak, fire, exposure or
products due to errors by ATCC will be replaced free of accident.
charge if reported within 30 days of receipt of the product(s).
1. Product Identification
Return Policy: CAS No.:
Hypoxanthine: 68-94-0
Authorization, including a "Return Authorization
Aminopterin: 54-62-6
Number" and shipping instructions must be obtained from the
Thymidine: 50-89-5
ATCC Sales Department prior to returning any item(s). Some
Molecular Weight: Not applicable to mixtures.
Products may not be returnable. The following information is Chemical Formula:
required: Hypoxanthine: (C5H4 N4O)
-Reason for return Aminopterin: (C19H20 N8 O5)
-Purchase Order Number Thymidine: (C10H14 N2O5)
-Sales Order Number 2. Composition/Information on Ingredients as rehydrated:
American Type Culture Collection Ordering info: 800-638-6597 (US and Canada)
P.O. Box 1549 703-365-2700 (elsewhere); Fax: 703-365-2750
Manassas, VA 20108 E-mail: sales@atcc.org; Web site: www.atcc.org
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Product Information Sheet for ATCC庐 69-X
Ingredient CAS No Percent Hazardous Do not breathe dust.
Hypoxanthine 68-94-0 ~0.136% N/A Do not get in eyes, on skin, on clothing.
Aminopterin 54-62-6 ~0.00176% N/A Avoid prolonged or repeated exposure.
Thymidine 50-89-5 ~0.0378% N/A Wash thoroughly after handling.
Keep tightly closed.
Store in a cool dry place.
3. Hazards Identification
Use only in a chemical fume hood.
Emergency Overview
As part of good industrial and personal hygiene and safety Wash contaminated clothing before reuse.
procedure, avoid all unnecessary exposure to the chemical
substance and ensure prompt removal from skin, eyes and 9. Physical and Chemical Properties
clothing. Appearance: White powder.
Melting Point: Hypoxanthine > 300掳C
Label precautionary statements: Aminipterin
Melting Point: Aminopterin 230掳C
Highly toxic (USA)
Melting Point: Thymidine 186掳C
Very toxic (EU)
May cause harm to the unborn child
10. Stability and Reactivity: Hypoxanthine
Very toxic by inhalation, in contact with skin and if
Hazardous Decomposition Products: Toxic fumes of:
swallowed
Carbon monoxide, carbon dioxide, nitrogen oxides.
Possible mutagen
Incompatibilities: Strong oxidizing agents.
Target organ(s):
Stability and Reactivity: Aminopterin
Blood
Hazardous Decomposition Products: Toxic fumes of:
Label precautionary statements: Thymidine
Carbon monoxide, carbon dioxide, nitrogen oxides.
Caution:
Incompatibilities: Strong oxidizing agents.
Avoid contact and inhalation
Hazardous polymerization: Will not occur.
4. First Aid Measures Stability and Reactivity: Thymidine
Inhalation: Remove to fresh air. If not breathing give artificial Stability: Stable
respiration. If breathing is difficult, give oxygen. Incompatibilities: Strong oxidizing agents
Ingestion: Wash out mouth with water provided person is Hazardous combustion or decomposition products: Carbon
conscious. Call a physician. monoxide, carbon dioxide, and nitrogen oxides
Skin Contact: In case of contact, immediately wash skin with Hazardous polymerization: Will not occur.
plenty of soap and water for at least 15 minutes. Remove
contaminated clothing and shoes. Wash clothing before reuse.
Get medical attention if irritation develops or persists. 11. Toxicological Information: Hypoxanthine:
Eye Contact: In case of contact, flush eyes with plenty of water Acute effects
for at least 15 minutes. Get medical advice if irritation develops. May be harmful by inhalation, ingestion, or skin absorption.
May cause irritation.
To the best of our knowledge, the chemical, physical, and
5. Fire Fighting Measures
Special Fire Fighting Procedures: Wear self-contained toxicological properties have not been thoroughly
breathing apparatus and protective clothing to prevent contact investigated.
with skin and eyes. RTECS #: UP0791000
Fire Extinguishing Media: Water spray, carbon dioxide, dry 6H-purin-6-one, 1,7-dihydro-
chemical or appropriate foam. Toxicity data
Unusual Fire and Explosions Hazards: Emits toxic fumes IPR-MUS LD50:750 MG / KG NTIS** AD691-490
under fire conditions. Target organ data
Effects on embryo or fetus (fetotoxicity)
Effects on embryo of fetus (fetal death)
6. Accidental Release Measures
Wear respirator, chemical safety goggles, rubber boots and Specific developmental abnormalities (craniofacial)
heavy rubber gloves. Specific developmental abnormalities (musculoskeletal
Sweep up, place in a bag and hold for waste disposal. system)
Avoid raising dust. Only selected registry of toxic effects of chemical substances
Ventilate area and wash spill site after material pickup is (RTECS) data is presented here. See actual entry in RTECS for
complete. complete information.
Toxicological Information: Aminopterin
Acute effects:
7. Handling and Storage
Refer to section 8. May cause skin irritation.
May cause eye irritation.
Material may be irritating to mucous membranes and upper
8. Exposure Controls/Personal Protection
Chemical safety goggles. respiratory tract.
Compatible chemical-resistant gloves. May be fatal if inhaled, swallowed, or absorbed through the
NIOSH / MSHA 鈥? approved respirator. skin.
Safety shower and eye bath. Blood effects:
Mechanical exhaust required.
American Type Culture Collection Ordering info: 800-638-6597 (US and Canada)
P.O. Box 1549 703-365-2700 (elsewhere); Fax: 703-365-2750
Manassas, VA 20108 E-mail: sales@atcc.org; Web site: www.atcc.org
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Product Information Sheet for ATCC庐 69-X
To the best of our knowledge, the chemical, physical, and RTECS #: XP2071000
toxicological properties have not been thoroughly Thymidine
investigated. Toxicity data
Chronic effects: IPR-MUS LD50:2512 MG / KG JPETAB 207, 504, 1978
May cause congenital malformation in the fetus. Target organ data
Target organ(s): Effects on embryo or fetus (fetotoxicity)
Blood Specific developmental abnormalities (musculoskeletal system)
RTECS #: MA1050000 Only selected registry of toxic effects of chemical substances
Glutamic acid, N-(P-(((2,4-Diamino-6- (RTECS) data is presented here. See actual entry in RTECS for
Pteridinyl)Methyl)Amino)benzoyl)-,L- complete information.
Toxicity data
IPR-RAT LD50:3 MG/KG 12. Ecological Information
ORL-MUS LD50:3 MG/KG Data not yet available.
IPR-MUS LD50:1900 UG/KG
Target organ data 13. Disposal Considerations
Behavioral (anorexia, human) Dissolve or mix the material with a combustible solvent and
Gastrointestinal (hypermotility, diarrhea) burn in a chemical incinerator equipped with an afterburner and
Gastrointestinal (other changes) scrubber.
Blood (leukemia) Observe all Federal, State and Local environmental regulations.
Skin and appendages (hair)
Paternal effects (spermatogenesis) 14. Transport Information
Effect on fertility (female fertility index) Not regulated.
Effect on fertility (post-implantation mortality)
Effect on fertility (abortion) 15. Regulatory Information: Hypoxanthine
Effect on embryo or fetus (extra embryonic structures) Reviews, standards, and regulations
Effect on embryo or fetus (fetotoxicity) OEL=MAK
Effect on embryo or fetus (fetal death) NOES 1983: HZD X6051; NIS 2; TNF 64; NOS 5; TNE
Specific developmental abnormalities (central nervous 541; TFE 355
system) EPA TSCA section 8 (B) chemical inventory
Specific developmental abnormalities (eye, ear) Regulatory Information: Aminopterin
Specific developmental abnormalities (craniofacial) European information
Specific developmental abnormalities (body wall) Very toxic
Specific developmental abnormalities (musculoskeletal R 61
system) May cause harm to the unborn child.
Specific developmental abnormalities (homeostasis) R 26/27/28
Specific developmental abnormalities (other developmental Very toxic by inhalation, in contact with skin and if
abnormalities) swallowed.
Effects on newborn (live birth index) S 45
Effects on newborn (APGAR score) In case of accident or if you feel unwell, seek medical advice
Effects on newborn (viability index) immediately (show the label where possible).
Effects on newborn (growth statistics) S 36/37/39
Effects on newborn (physical) Wear suitable protective clothing, gloves and eye/face
Nutritional and gross metabolic (weight loss or decreased protection.
weight gain) S 22
Tumorigenic (equivocal tomorigenic agent by RTECS Do not breathe dust.
criteria) Reviews, standards, and regulations
Only selected registry of toxic effects of chemical substances OEL=MAK
(RTECS) data is presented here. See actual entry in RTECS for EPA genetox program 1988, positive: mammalian
complete information. micronucleus; sperm morphology-mouse
EPA genetox program 1988, positive: S cervisiae-
homozygosis
Toxicological Information: Thymidine
Acute effects: EPA genetox program 1988, negative: N crassa-aneuploidy
May cause skin irritation. EPA TSCA section 8 (B) chemical inventory
May be harmful if absorbed through the skin. U.S. information
This product is subject to SARA section 313 reporting
May cause eye irritation. requirements.
May be harmful if inhaled. California proposition 65:
Material may be irritating to mucous membranes and upper This product is or contains chemical(s) known to the state of
respiratory tract. California to cause female reproductive toxicity
May be harmful if swallowed. Regulatory Information: Thymidine
To the best of our knowledge, the chemical, physical, and Reviews, standards, and regulations
toxicological properties have not been thoroughly investigated. OEL=MAK
American Type Culture Collection Ordering info: 800-638-6597 (US and Canada)
P.O. Box 1549 703-365-2700 (elsewhere); Fax: 703-365-2750
Manassas, VA 20108 E-mail: sales@atcc.org; Web site: www.atcc.org
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Product Information Sheet for ATCC庐 69-X
NOES 1983: HZD X7245; NIS 2; TNF 213; NOS 3; TNE
578; TFE 141
EPA genetox program 1988, positive: V79 cell culture-gene
mutation
EPA genetox program 1988, negative: cell transform. 鈥?
C3H/10T1/2
EPA genetox program 1988, inconclusive; carcinogenicity-
mouse/rat
EPA TSCA section 8(B) chemical inventory
EPA TSCA section 8(D) unpublished health / safety studies
EPA TSCA test submission (TSCATS) data base, Deember
1999
16. Other Information
The above information is believed to be correct but does not
purport to be all-inclusive and shall be used only as a guide.
This document is intended only as a guide to the appropriate
precautionary handling of the material by a person trained in, or
supervised by a person trained in, chemical handling. The user is
responsible for determining the precautions and dangers of this
chemical for his or her particular application. Depending on usage,
protective clothing including eye and face guards and respirators
must be used to avoid contact with material or breathing chemical
vapors/fumes.
American Type Culture Collection Ordering info: 800-638-6597 (US and Canada)
P.O. Box 1549 703-365-2700 (elsewhere); Fax: 703-365-2750
Manassas, VA 20108 E-mail: sales@atcc.org; Web site: www.atcc.org
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