Material Safety Data Sheet
Revision: 01/23/2006
Ethanol Guanidine Guanidine
Hazard information is provided for compliance with both the UK Chemicals
Thiocyanate and Hydrochloride
(Hazard Information and Packaging) (CHIP) Regulations and the US Hazard
Guanidine
Communication Standard (HCS) Hydrochloride
IDENTIFICATION OF THE PRODUCT NAME: PRODUCT CODE: EEC NUMBER:
PrepEaseTM Plant RNA Spin Kit
SUBSTANCE/PREPARATION 78770 / 78771 None
AND COMPANY
SUPPLIER: Emergency Contact:
USB Corporation, 26111 Miles Road, Cleveland, Ohio 44128 Phone: (216) 765-5000 Chemtrec (800) 424-9300
Please visit our website at www.usbweb.com for contact information on USB Outside USA & Canada 703 527 3887
product distributors within your area.
COMPOSITION/
HAZARDOUS COMPONENTS HAZARD CAS NO. %WT TLV CHIP R & S Phrases
Guanidine Thiocyanate 593-84-0 <60% 鈥? R:20/21/22 Harmful by inhalation, in
(RA1, RA2 and MDB contact with skin and if swallowed.
Buffers) R:32 Contact with acids liberates very
toxic gas.
S:13 Keep away from food, drink and
animal feeding stuffs.
Guanidine Hydrochloride 50-01-1 <45% 鈥? R:22 Harmful if swallowed.
(RAP Buffer) R:36/38 Irritating to eyes and skin.
S:23 Do not breathe vapour.
Ethanol 64-17-5 <30% See R:10 Flammable.
(RA2 and MDB Buffers) 鈥淩egulatory S:7 Keep container tightly closed.
Information鈥? S:16 Keep away from sources of
Section ignition - No smoking.
HAZARDS IDENTIFICATION CHIP
Flammable (Ethanol); Harmful (Guanidine Thiocyanate and Guanidine HCl); Irritant (Guanidine HCl)
HCS
Flammable Liquid (Ethanol); Irritant (Guanidine Thiocyanate and Guanidine HCl)
FIRST-AID MEASURES EYES: Flush with water for 15 minutes. Seek medical advice if irritation persists.
SKIN: Flush with water, then wash thoroughly with soap and water. Remove contaminated clothing and wash
before reuse. Seek medical attention if irritation persists.
INHALATION: Remove the victim from exposure and move to fresh air. If breathing is difficult, give oxygen. If
not breathing, give artificial respiration. Keep victim quiet and warm. Seek immediate medical attention.
INGESTION: Drink water and seek immediate medical attention. Avoid alcoholic beverages. Never give
anything by mouth to an unconscious person.
ANTIDOTE: Always have a cyanide antidote kit on hand when working with cyanide compounds. Obtain
medical advice on proper use.
FIRE-FIGHTING INFORMATION Use media suitable to extinguish the supporting or surrounding fire. Wear NIOSH (or equivalent) approved self
contained breathing apparatus. For small fires only: use carbon dioxide, dry powder or foam. Flammable liquid.
Vapor may travel considerable distance to source of ignition and flash back. Emits toxic fumes under fire
conditions. Explosion Limits (Pure Ethanol): Lower = 3.3% Upper = 19%.
Flash Point = ~32掳C (RA2 and MDB Buffers). Autoignition Temperature = 363掳C (Pure Ethanol).
ACCIDENTAL RELEASE MEASURES Wear appropriate personal protective equipment and clothing including lab coat, safety glasses, gloves and NIOSH-
approved respirator. Eliminate all sources of ignition. Cover with dry-lime, sand or soda ash. Using non-sparking
tools, collect in a manner that does not create dust and place in a suitable waste container. Avoid contact of
material with skin or eyes. Use adequate ventilation.
HANDLING AND STORAGE Wear appropriate personal protective equipment and clothing including lab coat, safety glasses, gloves and NIOSH-
approved respirator. Avoid contact of material with skin or eyes. Use adequate ventilation. Avoid heat, sparks and
open flame. Keep away from all sources of ignition. Ground all equipment containing material. Store ambient away
from incompatible materials.
PERSONAL PROTECTION Wear appropriate personal protective equipment and clothing including lab coat, safety glasses, gloves and NIOSH-
approved respirator. A qualified industrial hygienist should evaluate the need for respiratory protection. Use
respiratory protection approved by NIOSH (or equivalent) and appropriate to the hazard. Avoid contact of material
with skin or eyes. Mechanical ventilation or local exhaust as needed to control exposure to dust, vapors or mists.
Access to a safety shower and eye-wash.
MSDS information may be continued on back of page.
PHYSICAL AND CHEMICAL Appearance: Kit containing vials of solution Boiling Point: No data available
PROPERTIES Vapor Pressure: No data available Vapor Density: No data available
Solubility (Water): Soluble Specific Gravity: No data available
Percent Volatile: No data available Evaporation Rate: No data available
Chemical Formula: Kit
STABILITY AND REACTIVITY Product is stable. Avoid high temperatures and sources of ignition. Hazardous decomposition products include
hydrogen cyanide, ammonia, hydrogen chloride gas and oxides of carbon, nitrogen and sulfur. Incompatible with
metals, tin, aluminum, zinc, iron, strong acids, alkali metals, ammonia, peroxides and strong oxidizing agents.
Heating to decomposition or contact with acids or acid vapors can liberate poisonous cyanide vapors. Hazardous
polymerization will not occur.
TOXICOLOGICAL INFORMATION EFFECTS OF OVEREXPOSURE:
FOR ETHANOL:
EYES: Contact may cause irritation. May cause chemical conjunctivitis and corneal damage.
SKIN: May be harmful if absorbed through the skin. Contact may cause irritation. Ethanol may have a drying or
irritating effect on the skin after repeated or prolonged exposure.
INHALATION: May be harmful if inhaled. Material is irritating to mucous membranes and upper respiratory tract.
Inhalation of high concentrations may cause central nervous system effects characterized by nausea, headache,
dizziness, unconsciousness and coma.
INGESTION: May be harmful if ingested. May cause gastrointestinal irritation with nausea, vomiting and diarrhea.
May cause CNS depression, characterized by excitement, followed by headache, dizziness, drowsiness and nausea.
Advanced stages may cause collapse, unconsciousness, coma and possible death.
TARGET ORGAN(S): Kidneys, Heart, CNS and Liver.
FOR GUANIDINE HYDROCHLORIDE:
EYES: Vapors may cause irritation or burning upon contact.
SKIN: May be harmful if absorbed through the skin. Causes severe irritation to skin and hypersensitive individuals
may experience an allergic reaction.
INHALATION: May be harmful if inhaled. May cause irritation to mucous membranes and upper respiratory tract.
INGESTION: Harmful if swallowed. Chronic ingestion or excessive dosage may cause gastrointestinal tract irritation
with nausea, vomiting and diarrhea. May cause central nervous system disorders.
TARGET ORGAN(S): Bone Marrow and Nerves.
FOR GUANIDINE THIOCYANATE:
EYES: Contact may cause irritation and slight corneal injury.
SKIN: Harmful if absorbed through skin. If absorbed, may cause symptoms similar to those for ingestion. Contact
may cause irritation and/or allergic reaction.
INHALATION: Harmful if inhaled. May cause similar effects as those described under ingestion. May cause irritation
to mucous membranes and upper respiratory tract. Exposure to low and even unmeasurable isocyanate
concentration may result in sensitization.
INGESTION: Harmful if swallowed. Chronic ingestion or excessive dosage may cause gastrointestinal tract irritation
with nausea, vomiting and diarrhea. Ingestion of thiocyanates may also cause disorientation, weakness, low blood
pressure, confusion, psychotic behavior, muscular spasm, convulsions and possibly death.
TARGET ORGAN(S): Kidneys, CNS, Liver, Lungs and Cardiovascular System.
ADDITIONAL INFORMATION:
May cause liver and kidney damage. May cause lung damage. May cause central nervous system depression.
Repeated or prolonged exposure may cause allergic reactions in sensitive individuals. Inhalation may aggravate
existing chronic respiratory problems such as asthma, emphysema or bronchitis.
Only select RTECS information is provided here. Please see actual RTECS entries for complete information.
Reproductive, irritation, mutagen, tumorigenic and toxicity data for Ethanol listed in RTECS under KQ6300000.
Irritation data: Skin Rabbit 20 mg/24H = Moderate (1986). Eye Rabbit 500 mg/24H = Mild (1986).
Toxicity data: Oral Rat LD50 = 7060 mg/kg (1970). Inhalation Rat LC50 = 20000 ppm/10H (1974).
Tumorigenic data: equivocal tumorigenic agent by RTECS criteria (1981).
Carcinogenic data: IARC Cancer Review: Animal Inadequate Evidence (1988).
ACGIH TLV-Not classifiable as a human carcinogen (2005).
Toxicity data for Guanidine Thiocyanate listed in RTECS under XL1225000.
Intraperitoneal Mouse LD50 = 300 mg/kg. Details of toxic effects not reported other than lethal dose value.
Irritation, mutation and toxicity data for Guanidine Hydrochloride listed in RTECS under MF4300000.
Irritation data: Skin Rabbit 500 mg/24H = Severe. Eye Rabbit 81400 ug = Moderate.
Toxicity data: Oral Rat LD50 = 475 mg/kg.
Definition(s): RTECS = Registry of Toxic Effects of Chemical Substances.
ACGIH = American Conference of Governmental Industrial Hygienists.
ECOLOGICAL INFORMATION No information available.
DISPOSAL CONSIDERATIONS Dispose of material in accordance with applicable local, state, and federal regulations.
TRANSPORTATION INFORMATION US DOT / IATA: Alcohols, n.o.s. (Ethanol Solution), UN1987, class 3, PG III, Label: Flammable liquid.
REGULATORY INFORMATION RCRA - No applicable information.
SARA 302 - No applicable information.
SARA 313 - No applicable information.
EPA TSCA Section 8(b) - All listed components: Chemical Inventory.
Exposure Limits - For Ethanol: ACGIH TLV-TWA: 1000 ppm. OSHA PEL (Gen Indu): 8H TWA 1000 ppm.
California Proposition 65 - No applicable information.
This data sheet is based upon information believed to be reliable. The Company makes no statement or warranty as to the accuracy or completeness of the
information contained herein which is offered for your consideration, investigation and verification. Any use of the information contained in this data sheet
must be determined by the user to be in accordance with appropriate applicable regulations.
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