Material Safety Data Sheet
Revision: 08/30/2007
N
2-Mercaptoethanol Potassium Acetate 2-Mercaptoethanol
Hazard information is provided for compliance with both the UK Chemicals
and Glycerol
(Hazard Information and Packaging) (CHIP) Regulations and the US Hazard
Communication Standard (HCS)
IDENTIFICATION OF THE PRODUCT NAME: PRODUCT CODE: EEC NUMBER:
SUBSTANCE/PREPARATION PrepEase庐 Yeast Plasmid Isolation Kit 79220 None
AND COMPANY
SUPPLIER: Emergency Contact:
USB Corporation, 26111 Miles Road, Cleveland, Ohio 44128 Phone: (216) 765-5000 Chemtrec (800) 424-9300
Please visit our website at www.usbweb.com for contact information on USB Outside USA & Canada 703 527 3887
product distributors within your area.
COMPOSITION/
HAZARDOUS COMPONENTS HAZARD CAS NO. %WT TLV CHIP R & S Phrases
Glycerol 56-81-5 ~50.0% OSHA TWA: R:36/37/38 Irritating to eyes, respiratory
in 79226 15 mg/m3 system and skin.
2-Mercaptoethanol 60-24-2 1.0% R:22 Harmful if swallowed.
in 79227 R:24 Toxic in contact with skin.
R:34 Causes burns.
R:51/53 Toxic to aquatic organisms, may
cause long term adverse effects in the
aquatic environment.
S:61 Avoid release to the environment.
Refer to special instructions/safety data
sheet.
Potassium Acetate 127-08-2 ~78.5% R:36/37/38 Irritating to eyes, respiratory
in 79229 system and skin.
For all kit S:26 In case of contact with eyes, rinse
components: immediately with plenty of water and seek
medical attention.
S:36/37/39 Wear suitable protective
clothing, gloves and eye/face protection.
S:45 In case of accident or if you feel
unwell, seek medical advice immediately
(show the label where possible).
HAZARDS IDENTIFICATION CHIP
2-Mercaptoethanol: Toxic, Dangerous for the Environment; Glycerol and Potassium Acetate: Irritant
HCS
2-Mercaptoethanol: Toxic; Glycerol and Potassium Acetate: Irritant
FIRST-AID MEASURES EYES: Flush with water for 15 minutes. Seek medical advice if irritation persists.
SKIN: Flush with water, then wash thoroughly with soap and water. Remove contaminated clothing and wash
before reuse. Seek medical attention if irritation persists.
INHALATION: Remove the victim from exposure and move to fresh air. If breathing is difficult, give oxygen. If
not breathing, give artificial respiration. Keep victim quiet and warm. Seek immediate medical attention.
INGESTION: Drink water and seek immediate medical attention. Avoid alcoholic beverages. Never give
anything by mouth to an unconscious person.
FIRE-FIGHTING INFORMATION Use media suitable to extinguish the supporting or surrounding fire. Wear NIOSH (or equivalent) approved self
contained breathing apparatus. For small fires only: use carbon dioxide, dry powder or foam. Emits toxic fumes
under fire conditions. For 2-Mercaptoethanol: Combustible Liquid. For Glycerol: Contact with strong oxidizing
agents may produce an explosion. Explosion limits: For 2-Mercaptoethanol - Lower: 1.1 Upper: 2.7;
For Glycerol - Lower: 1.1 Upper: Not available.
Flash Point: For 2-Mercaptoethanol = 74掳C; For Glycerol = 193掳C.
Autoignition Temperature: For 2-Mercaptoethanol =295掳C; For Glycerol = 400掳C
ACCIDENTAL RELEASE MEASURES Wear appropriate personal protective equipment and clothing including lab coat, safety glasses, gloves and NIOSH-
approved respirator. Eliminate all sources of ignition. Collect in a manner that does not create dust and place in a
suitable waste container. Avoid contact of material with skin or eyes. Use adequate ventilation.
MSDS information may be continued on back of page.
HANDLING AND STORAGE Wear appropriate personal protective equipment and clothing including lab coat, safety glasses, gloves and NIOSH-
approved respirator. Avoid contact of material with skin or eyes. Use adequate ventilation. Store in a cool, dry,
well-ventilated area away from incompatible substances. For 2-Mercaptoethanol: Moisture sensitive. Keep away
from heat, sparks and flame. For Potassium Acetate: Hygroscopic.
PERSONAL PROTECTION Wear appropriate personal protective equipment and clothing including lab coat, safety glasses, gloves and NIOSH-
approved respirator. A qualified industrial hygienist should evaluate the need for respiratory protection. Use
respiratory protection approved by NIOSH (or equivalent) and appropriate to the hazard. Avoid contact of material
with skin or eyes. Mechanical ventilation or local exhaust as needed to control exposure to dust, vapors or mists.
Access to a safety shower and eye-wash.
PHYSICAL AND CHEMICAL Appearance: Kit containing separate vials Evaporation Rate: No data available
PROPERTIES Boiling Point: For Glycerol=288掳C; 2-Mercaptoethanol=157掳C Percent Volatile: No data available
Specific Gravity: For Glycerol=1.26; 2-Mercaptoethanol=1.14 Vapor Density: For 2-Mercaptoethanol=2.69
Vapor Pressure: For Glycerol=.0025 mm Hg@5; 2-Mercaptoethanol=1 mm Hg@ 20掳C
Melting Point: For Glycerol=20掳F; For 2-Mercaptoethanol=-50掳C; For Potassium Acetate=292掳C
STABILITY AND REACTIVITY Product is stable under normal conditions. Hazardous polymerization will not occur. Hazardous decomposition
products include carbon oxides, oxides of sulfur and irritating and toxic fumes and gases. Conditions to Avoid: For
2-Mercaptoethanol avoid high temperatures, ignition sources and excess heat; For Potassium Acetate avoid excess
heat and exposure to moist air or water. Incompatibilities: For 2-Mercaptoethanol avoid strong oxidizers, calcium
hypochlorite, metals , strong acids, caustics, aliphatic amines and isocyanates; For Potassium Acetate avoid
moisture; For Glycerol avoid strong oxidizing agents including mixtures with hydrogen peroxide, potassium
permanganate, calcium hypochlorite, nitric acid, sulfuric acid, perchloric acid and lead oxide. Contact with Sodium
Hypochlorite and Hypochlorous acid may cause an explosion.
TOXICOLOGICAL INFORMATION EFFECTS OF OVEREXPOSURE: The toxicological properties of the PrepEase庐 Yeast Plasmid Isolation Kit have not
been thoroughly investigated. Handle according to the precautions for the most hazardous component.
FOR GLYCEROL: Oral Rat LD50 = 12600 mg/kg.
EYES: Contact may cause irritation and slight corneal injury.
SKIN: Prolonged contact may cause irritation and/or allergic reaction.
INHALATION: No known toxicity, but excessive fumes may cause irritation if inhaled.
INGESTION: May cause irritation of gastrointestinal tract and diarrhea.
Irritation, mutation, reproductive effects and toxicity data for Glycerol is listed in RTECS under MA8050000.
FOR 2-MERCAPTOETHANOL: Oral Rat LD50 = 244 mg/kg. Inhalation Rat LCLo = 250 ppm/8H.
EYES: May cause severe eye irritation. May result in corneal injury.
SKIN: May be fatal if absorbed through the skin. Causes irritation with burning pain, itching and redness.
Prolonged or repeated skin contact may cause defatting and dermatitis.
INHALATION: Causes respiratory tract irritation. Mercaptans may cause nausea and headache. Inhalation of
high concentrations may cause central nervous system effects characterized by nausea, headache,
dizziness, unconsciousness and coma.
INGESTION: Harmful if swallowed. May cause gastrointestinal irritation with nausea, vomiting and diarrhea.
May cause muscle paralysis, respiratory failure and possible death.
Irritation, mutation and toxicity data for 2-Mercaptoethanol listed in RTECS under KL5600000.
For POTASSIUM ACETATE: Oral Rat LD50 = 3250 mg/kg.
EYES: Causes eye irritation. May cause chemical conjunctivitis.
SKIN: Causes skin irritation.
INHALATION: Material is irritating to mucous membranes and upper respiratory tract.
INGESTION: May cause gastrointestinal irritation with nausea, vomiting and diarrhea.
Toxicity data for Potassium Acetate listed in RTECS under AJ3325000.
ECOLOGICAL INFORMATION No information available.
DISPOSAL CONSIDERATIONS Dispose of material in accordance with applicable local, state, and federal regulations.
TRANSPORTATION INFORMATION US DOT / IATA: No applicable information.
REGULATORY INFORMATION RCRA - No applicable information.
SARA 302 - This material does not have an RQ or TPQ.
SARA 313 - This material is not reportable under 313.
EPA TSCA Section 8(b) - For Glycerol, Potassium Acetate and 2-Mercaptoethanol: Chemical Inventory.
8(d) - For Glycerol: unpublished health/safety studies.
Exposure Limits - For Glycerol: ACGIH TLV TWA: 10 mg/m3 (total particulate).
OSHA PEL TWA: 15 mg/m3 (total dust).
California Proposition 65 - No applicable information.
This data sheet is based upon information believed to be reliable. The Company makes no statement or warranty as to the accuracy or completeness of the
information contained herein which is offered for your consideration, investigation and verification. Any use of the information contained in this data sheet
must be determined by the user to be in accordance with appropriate applicable regulations.
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