SIGMA-ALDRICH
Material Safety Data Sheet
Date Printed: 17/JUN/2005
Date Updated: 21/MAR/2004
Version 1.4
According to 91/155/EEC
Classified as Hazardous according to the criteria of EU Annex 1 and
NOHSC.
1 - Product and Company Information
Product Name NITRO BLUE TETRAZOLIUM GRADE III
CRYSTALLINE
Product Number N6876
Company Sigma-Aldrich Pty. Ltd.
12 Anella Avenue
Castle Hill NSW 2154
Australia
Technical Phone # +61 2 9841 0555 (1800 800 097)
Fax +61 2 9841 0500 (1800 800 096)
Emergency Phone # +44 8701906777 (1800 448 465)
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
NITRO BLUE TETRAZOLIUM None 206-067-4 603-001-00-X
Ingredient Name Percent CAS # EC no Annex I
THE FOLLOWING SUBSTANCES None None None
ARE PRESENT AS RESIDUAL
COMPONENTS OF PRODUCTION.
METHANOL >= 3 67-56-1 200-659-6 603-001-00-X
<= 10
Symbols: F T
R-Phrases: 11 23/24/25 39/23/24/25
Highly flammable. Toxic by inhalation, in contact with skin and if
swallowed. Toxic: danger of very serious irreversible effects
through inhalation, in contact with skin and if swallowed.
Formula C40H30Cl2N10O6
Molecular Weight 817.7 AMU
Synonyms NBT (dye) * Nitro Blue tetrazolium * p-Nitro
Blue tetrazolium * Nitro Blue tetrazolium
chloride * p-Nitro Blue tetrazolium chloride *
Nitro BT * Nitrotetrazolium Blue *
p-Nitrotetrazolium Blue * Nitro tetrazolium BT *
Nitrotetrazolium Chloride Blue * Nitro Blue
tetrazolium salt * Tetrazolium Nitro Blue *
Tetrazolium nitro BT
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
Harmful by inhalation, in contact with skin and if swallowed.
Harmful: possible risk of irreversible effects through inhalation,
in contact with skin, and if swallowed.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If not breathing give
artificial respiration. If breathing is difficult, give oxygen.
AFTER SKIN CONTACT
In case of skin contact, flush with copious amounts of water for
at least 15 minutes. Remove contaminated clothing and shoes.
Call a physician.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is
conscious. Call a physician.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Carbon dioxide, dry chemical powder, or appropriate
foam.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing
to prevent contact with skin and eyes.
6 - Accidental Release Measures
PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid
raising dust. Ventilate area and wash spill site after material
pickup is complete.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Do not breathe vapor. Avoid
contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure. Do not use if skin is cut or scratched. Wash
thoroughly after handling.
STORAGE
Conditions of Storage: Keep tightly closed.
Store at 2-8掳C
8 - Exposure Controls / Personal Protection
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ENGINEERING CONTROLS
Safety shower and eye bath. Mechanical exhaust required.
GENERAL HYGIENE MEASURES
Wash thoroughly after handling.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Government approved respirator.
Hand Protection: Compatible chemical-resistant gloves.
Eye Protection: Chemical safety goggles.
9 - Physical and Chemical Properties
Appearance Physical State: Solid
Color: Light yellow
Property Value At Temperature or Pressure
pH N/A
BP/BP Range N/A
MP/MP Range 189 掳C
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient N/A
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content N/A
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility Solvent: 50 mg/ml MeOH Clear, very strongly
yellow
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Materials to Avoid: Strong oxidizing agents.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide,
Nitrogen oxides, Hydrogen chloride gas.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
11 - Toxicological Information
RTECS NUMBER: XF8045000
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ACUTE TOXICITY
LD50
Oral
Mouse
2000 mg/kg
SIGNS AND SYMPTOMS OF EXPOSURE
Exposure can cause: Gastrointestinal disturbances. May cause
convulsions. To the best of our knowledge, the chemical,
physical, and toxicological properties have not been thoroughly
investigated.
ROUTE OF EXPOSURE
Skin Contact: May cause skin irritation.
Skin Absorption: Harmful if absorbed through skin.
Eye Contact: May cause eye irritation.
Inhalation: Harmful if inhaled. Material may be irritating to
mucous membranes and upper respiratory tract.
Ingestion: Harmful if swallowed.
TARGET ORGAN INFORMATION
Eyes. Kidneys. Liver. Heart.
12 - Ecological Information
No data available.
13 - Disposal Considerations
SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose
of this material. Dissolve or mix the material with a combustible
solvent and burn in a chemical incinerator equipped with an
afterburner and scrubber. Observe all federal, state, and local
environmental regulations.
14 - Transport Information
RID/ADR
Non-hazardous for road transport.
IMDG
Non-hazardous for sea transport.
IATA
Non-hazardous for air transport.
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
ANNEX I INDEX NUMBER: 603-001-00-X
INDICATION OF DANGER: Xn
Harmful.
R-PHRASES: 20/21/22 68/20/21/22
Harmful by inhalation, in contact with skin and if swallowed.
Harmful: possible risk of irreversible effects through
inhalation, in contact with skin, and if swallowed.
S-PHRASES: 36/37 45
Wear suitable protective clothing and gloves. In case of
accident or if you feel unwell, seek medical advice immediately
(show the label where possible).
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16 - Other Information
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2005 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
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