MATERIAL SAFETY DATA SHEET
According to 91/155 EEC
This material safety datasheet applies to Bio-X Diagnostics ELISA Kits.
1) Data on components :
The components included in the kit and defined as hazardous chemicals according to the Directive 67/548/EEC
are :
ProClin at a final concentration of 0,01-0,1% used in Positive reference, Dilution buffer, Washing
-
solution and microplates
Phosphoric acid : at a final concentration of 1N in aqueous solution used in stopping solution
-
Tetramethylbenzidine (TMB) used in Chromogen
-
Other components included in the Bio-X Diagnostics ELISA are not considered as hazardous chemical according to
EC Directives.
PROCLIN
SIGMA ALDRICH
Date Printed: 01/JUL/2004
Date Updated: 16/JUN/2004
Version 1.11
According to 91/155/EEC
1 - Product and Company Information
Product Name PROCLIN 300 50ML
Product Number 48126
Emergency Phone # 070 245245
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
PROCLIN(TM) 300 None None None
Ingredient Name Percent CAS # EC no Annex I
Index Number
GLYCOLS >= 91 None None None <= 97
MODIFIED ALKYL >= 1 None None None CARBOXYLATE 3.6
5-CHLORO-2-METHYL-4-ISOTH>= 2.1 26172-55-4 247-500-7 None
IAZOLIN-3-ONE <= 2.9
Symbols: C N
R-Phrases: 34 43 50
Causes burns. May cause sensitization by skin contact. Very toxic to aquatic organisms.
2-METHYL-4-ISOTHIAZOLIN-3>= 0.6 2682-20-4 None None -ONE <= 1.1
Symbols: C
R-Phrases: 34 43
Causes burns. May cause sensitization by skin contact.
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
Causes burns. May cause sensitization by skin contact.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give oxygen.
AFTER SKIN CONTACT
In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing
and shoes. Call a physician.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is conscious. Call a physician. Do not induce vomiting.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Use extinguishing media appropriate to surrounding fire conditions.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions. Combustible liquid.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
6 - Accidental Release Measures
PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy rubber gloves.
ENVIRONMENTAL PRECAUTION(S)
Avoid contaminating sewers and waterways with this material.
METHODS FOR CLEANING UP
Cover with dry-lime, sand, or soda ash. Place in covered containers using non-sparking tools and transport outdoors.
Ventilate area and wash spill site after material pickup is complete.
ADDITIONAL INFORMATION
Decontaminate spill area with a solution of 10% sodium bisulfite in water. Rinse decontaminated solution to a
chemical sewer after standing for 30 minutes.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Do not breathe vapor. Do not get in eyes, on skin, on clothing. Avoid prolonged or
repeated exposure.
STORAGE
Conditions of Storage: The maximum recommended storage temperature for this material is 55'C/131'F. The
minimum recommended storage temperature for this material is 0'C/32'F.
The product as supplied evolves gas (largely carbon dioxide) slowly. To prevent the buildup of pressure the product is
SUPELCO - 48126 www.sigma-aldrich.com Page 2 packaged in specially vented containers. Keep this product in the
original container when not in use. Container must be stored in an upright position to prevent spilling the contents
through the vent. Keep tightly closed. Keep away from heat and open flame.
8 - Exposure Controls / Personal Protection
ENGINEERING CONTROLS
Safety shower and eye bath. Use only in a chemical fume hood.
GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Discard contaminated shoes. Wash thoroughly after handling.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Government approved respirator.
Hand Protection: Compatible chemical-resistant gloves.
Eye Protection: Chemical safety goggles.
Special Protective Measures: Faceshield (8-inch minimum).
9 - Physical and Chemical Properties
Appearance Physical State: Liquid
Property Value At Temperature or Pressure
pH 4.1 Concentration: 100 g/l
BP/BP Range 189 掳 C
MP/MP Range -40 掳 C
Flash Point > 66 掳 Method: closed cup
C
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure 0.06 mmHg
SG/Density 1.03 g/cm3
Partition Coefficient Log Kow: < 3
Viscosity 58.5 Pas 25 掳 C
Vapor Density > 1 g/l
Saturated Vapor Conc. N/A
Evaporation Rate < 1
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content N/A
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility Solubility in Water:Soluble.
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Materials to Avoid: Oxidizing agents, Reducing agents, Amines,
Mercaptans.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Nitrogen oxides, Sulfur oxides,
Hydrogen chloride gas.
SUPELCO - 48126 www.sigma-aldrich.com Page 3
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
11 - Toxicological Information
ACUTE TOXICITY
LD50
Oral
rat, female
3,723 mg/kg
LD50
Oral
rat, male
3,600 mg/kg
LD50
Skin
Rabbit
> 3,600 mg/kg
LD50
Skin
Rabbit
3,500 mg/kg
IRRITATION DATA
Skin
Rabbit
18.6 %
Remarks: Severe irritation effect
Skin
Rabbit
9.3 %
Remarks: Moderate irritation effect
Skin
Rabbit
1.8 %
Remarks: No irritation effect
Eyes
Rabbit
Remarks: Severe irritation effect
Eyes
Rabbit
9.3 %
Remarks: Moderate irritation effect
Eyes
Rabbit
1.8 %
Remarks: No irritation effect
Eyes
Rabbit
Remarks: Severe irritation effect
Skin
Rabbit
Remarks: Severe irritation effect
SENSITIZATION
Skin: May cause allergic skin reaction.
SIGNS AND SYMPTOMS OF EXPOSURE
Material is extremely destructive to tissue of the mucous membranes and upper respiratory tract, eyes, and skin.
Inhalation may result in spasm, inflammation and edema of the SUPELCO - 48126 www.sigma-aldrich.com Page 4
larynxand bronchi, chemical pneumonitis, and pulmonary edema.
Symptoms of exposure may include burning sensation, coughing, wheezing, laryngitis, shortness of breath,
headache, nausea, and vomiting. Exposure can cause: Dermatitis. To the best of our
knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated.
ROUTE OF EXPOSURE
Skin Contact: Causes burns.
Skin Absorption: May be harmful if absorbed through the skin.
Eye Contact: Causes burns.
Inhalation: Material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract. May
be harmful if inhaled.
Ingestion: May be harmful if swallowed.
12 - Ecological Information
No data available.
13 - Disposal Considerations
SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose of this material. Observe all federal, state, and local
environmental regulations. This combustible material may be burned in a chemical incinerator equipped with an
afterburner and scrubber.
14 - Transport Information
RID/ADR
UN#: 3265
Class: 8
PG: II
Proper Shipping Name: Corrosive liquid, acidic,
organic, n.o.s.
IMDG
UN#: 3265
Class: 8
PG: II
Proper Shipping Name: CORROSIVE LIQUID, ACIDIC,
ORGANIC, N.O.S.
Marine Pollutant: No
Severe Marine Pollutant: No
Technical Name: Required
IATA
UN#: 3265
Class: 8
PG: II
Proper Shipping Name: Corrosive liquid, acidic,
organic, n.o.s.
Inhalation Packing Group I: No
Technical Name: Required
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
INDICATION OF DANGER: C
Corrosive.
SUPELCO - 48126 www.sigma-aldrich.com Page 5
R-PHRASES: 34 43
Causes burns. May cause sensitization by skin contact.
S-PHRASES: 26 36/37/39 45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective
clothing, gloves, and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately
(show the label where possible).
COUNTRY SPECIFIC INFORMATION
Germany
WGK: 2 (Self classification)
16 - Other Information
WARRANTY
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a
guide. The information in this document is based on the present state of our knowledge and is applicable to the
product with regard to appropriate safety precautions. It does not represent any guarantee of the properties of the
product. Sigma-Aldrich Inc., shall not be held liable for any damage resulting from handling or from contact with the
above product. See reverse side of invoice or packing slip for additional terms and conditions of sale.
Copyright 2004 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
PHOSPHORIC ACID
VWR International
Date of issue: 14/03/02
1. Identification of the substance/preparation and of the company/undertaking
Identification of the product
Catalogue No: P20621 ID No.: 1017300
Product name: Orthophosphoric acid 84%, Rectapur
Use of the substance/preparation: General chemical reagent
Manufacturer/supplier identification
Company: VWR International Ltd.
Emergency telephone No.: + 44 (0) 1202 669700
2. Composition/information on ingredients
Chemical characterization
Acid
Product name: Orthophosphoric acid 25 - 98%
CAS number: Not applicable (Solution in water) EC-No.: 231-633-2
Molecular formula: H3PO4 = 98.00 g/mol
Hazardous ingredients:
Orthophosphoric acid >50%
CAS number: 7664-38-2 EC-No.: 231-633-2
Symbol(s): C
R-phrases: R34
S-phrases: S26-45
3. Hazards identification
Causes burns.
4. First aid measures
- Eye contact: Irrigate thoroughly with water for at least 10 minutes. OBTAIN MEDICAL ATTENTION.
- Inhalation: Remove from exposure, rest and keep warm. In severe cases, or if exposure has been great, OBTAIN
MEDICAL
ATTENTION.
- Skin contact: Drench the skin thoroughly with water. Remove contaminated clothing and wash before re-use. Unless
contact
has been slight, OBTAIN MEDICAL ATTENTION.
- Ingestion: Wash out mouth thoroughly with water and give plenty of water to drink. OBTAIN MEDICAL
ATTENTION.
5. Fire-fighting measures
Special risks:
Not combustible. May evolve toxic fumes in fire. (phosphorus oxides)
Suitable extinguishing media:
To suit environment.
Do not stay in dangerous zone without respiratory protective equipment. Prevent fire fighting water entering
watercourses or ground-water.
6. Accidental release measures
Wear appropriate protective clothing.
Spread soda ash liberally over the spillage. If local regulations permit, mop up cautiously with plenty of water and run
to waste, diluting greatly with running water. Otherwise transfer to container and arrange removal by disposal
company. Wash site of spillage thoroughly with water.
For large spillages liquids should be contained with sand or earth and both liquids and solids transferred to salvage
containers.
Any residues should be treated as for small spillages.
7. Handling and storage
Handling:
Avoid contact with skin and eyes. Change contaminated clothing. Wash hands after working with substance. Keep
away from alkalis.
Unsuitable working materials: various metals.
Storage:
Store at room temperature (15 to 25掳 recommended). Keep well closed and protected from direct sunlight and
C
moisture.
8. Exposure controls/personal protection
UK Exposure Limits:
OES - Orthophosphoric acid:
Long term: 1 mg/m3 Short term: 2 mg/m3 (IOELV)
Personal protective equipment:
As appropriate to the situation and the quantity handled. Engineering methods to control or prevent exposure are
preferred.
Methods could include process enclosure or mechanical ventilation.
- Ventilation: Fume cupboard required when vapours/mists are generated.
- Respirator: Self-contained breathing apparatus required when vapours/mists are generated.
- Gloves: Rubber or plastic. Gloves subject to permeation or any sign of degradation must be removed and replaced
immediately.
- Eye Protection: Goggles or face-shield
- Other Precautions: Plastic apron, sleeves, boots - if handling large quantities
9. Physical and chemical properties
General information:
Form: liquid
Colour: colourless
Odour: odourless
Health, safety and environmental information:
Melting temperature Not available
Boiling temperature Not available
Density(g/ml) ~1.7
Solubility in water Miscible in all proportions
pH value <1 (100g/l H2O)
Flash point Not applicable
Explosion limits: lower: Not applicable
Auto-ignition temperature Not applicable
10. Stability and reactivity
Stable.
Unsuitable working materials: iron/iron-containing compounds, steel, aluminium.
Substances to be avoided
bases, metals (Formation of: hydrogen), metallic oxides, organic nitro compounds.
The possibility of reaction with other substances cannot be excluded.
Hazardous decomposition products: phosphorus oxides.
11. Toxicological information
- After ingestion: burns, severe pain (risk of perforation!), shock, spasms.
- After skin contact: irritant and caustic effects.
- After eye contact: conjunctivitis, burns.
Further data
No data
We have no evidence of carcinogenic effects. We have no evidence of mutagenic or teratogenic effects.
12. Ecological information
Do not allow to enter drinking water supplies, waste water, or soil! Caustic even in diluted form. Harmful effect due to
pH shift.
Depending on the concentration, phosphorus compounds may contribute to the eutrophication of water supplies.
13. Disposal considerations
Chemical residues are generally classified as special waste, and as such are covered by regulations which vary
according to location. Contact your local waste disposal authority for advice, or pass to a chemical disposal company.
Rinse out empty containers thoroughly before returning for recycling.
14. Transport information
UN-No.: 1805 Class: 8 Packaging group: III
Proper shipping name: PHOSPHORIC ACID, LIQUID
15. Regulatory information
Labelling according to EC directives
Symbol(s): C Corrosive.
R-phrases: R34
Causes burns.
S-phrases: S26-36/37/39-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective
clothing, gloves, and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately
(show the label where possible).
EC-No.: 231-633-2
Local Regulations
U.K. Transport Category 3
Within the UK, the use of this material must be assessed under the Control of Substances Hazardous to Health
(COSHH) regulations.
16. Other information
Revision.
Supercedes issue of 10/07/01
Reason for alteration: Changes in Section : 8 (OES)
Date of issue: 14/03/02
Date of print: 08/04/05
Text of R phrases listed in section 2
R34: Causes burns.
TETRAMETHYLBENZIDINE
Date Printed: 18/JUN/2004
Date Updated: 20/MAR/2004
Version 1.3
According to 91/155/EEC
1 - Product and Company Information
Product Name 3,3',5,5'-TETRAMETHYLBENZIDINEDIHYDROCHLORIDE
Product Number T8768
Company Sigma-Aldrich N.V./S.A.
Emergency Phone # 070 245245
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
3,3',5,5'-TETRAMETHYLBENZIDINE 64285-73-0 264-769-6 None
DIHYDROCHLORIDE
Formula C16H20N2*2HCl
Molecular Weight 313.27 AMU
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
Not hazardous according to Directive 67/548/EC.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If breathing becomes difficult, call a physician.
AFTER SKIN CONTACT
In case of contact, immediately wash skin with soap and copious amounts of water.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is conscious. Call a physician.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or appropriate foam.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
6 - Accidental Release Measures
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Exercise appropriate precautions to minimize direct contact with skin or eyes and prevent inhalation of dust.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid raising dust. Ventilate area and wash spill site after
material pickup is complete.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.
STORAGE
Conditions of Storage: Keep tightly closed. Store under nitrogen.
Store at 2-8掳C
SPECIAL REQUIREMENTS: Air, light, and moisture sensitive. Handle under inert gas.
8 - Exposure Controls / Personal Protection
ENGINEERING CONTROLS
Safety shower and eye bath. Mechanical exhaust required.
GENERAL HYGIENE MEASURES
Wash thoroughly after handling.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Wear dust mask.
Hand Protection: Protective gloves.
Eye Protection: Chemical safety goggles.
9 - Physical and Chemical Properties
Appearance Physical State: Solid
Color: Faintly beige
Property Value At Temperature or Pressure
pH N/A
BP/BP Range N/A
MP/MP Range > 300 掳 C
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
SIGMA - T8768 www.sigma-aldrich.com Page 2
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient N/A
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content N/A
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility N/A
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Conditions to Avoid: Air, light, and moisture sensitive.
Materials to Avoid: Strong oxidizing agents.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide,
Nitrogen oxides, Hydrogen chloride gas.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
11 - Toxicological Information
SIGNS AND SYMPTOMS OF EXPOSURE
To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly
investigated.
ROUTE OF EXPOSURE
Skin Contact: May cause skin irritation.
Skin Absorption: May be harmful if absorbed through the skin.
Eye Contact: May cause eye irritation.
Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract.
Ingestion: May be harmful if swallowed.
12 - Ecological Information
13 - Disposal Considerations
SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix the material with a
combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Observe all
federal, state, and local environmental regulations.
14 - Transport Information
RID/ADR
Non-hazardous for road transport.
IMDG
SIGMA - T8768 www.sigma-aldrich.com Page 3
Non-hazardous for sea transport.
IATA
Non-hazardous for air transport.
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
S-PHRASES: 22 24/25
Do not breathe dust. Avoid contact with skin and eyes.
16 - Other Information
WARRANTY
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a
guide. The information in this document is based on the present state of our
knowledge and is applicable to the product with regard to appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc., shall not be held liable for any damage resulting from
handling or from contact with the above product. See reverse side of invoice or packing slip for additional terms and
conditions of sale.
Copyright 2004 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
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