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               Epstein Barr Virus (Latent Membrane Protein)                                                                        VP-E609

Antibody to                                                                                                            Cat. No.



                                                         Product Specifications



Description:                                                                     Applications:

Epstein Barr Virus (LMP) mouse monoclonal antibody

                                                                                 Immunohistochemistry

Immunoglobulin class:                   All clones: IgG1                         Paraffin sections:       Trypsin digestion recommended.

                                                                                 Frozen sections:         Yes

Clone: CS1, CS2, CS3 and CS4.

                                                                                 Working Dilution*:       1:100 - 1:200 for 1hr at 25 掳C.

                                                                                 Positive Control:        Lymph node, Hodgkin鈥檚 disease

Immunogen:        Fusion protein including sequences of

                                                                                                          (about 50% of cases are likely to

the EBV encoded latent membrane protein (LMP-1) and

                                                                                                          be positive). Cytoplasmic staining

bacterial beta-galactosidase.

                                                                                                          pattern may be observed in Reed

                                                                                                          Sternberg cells.

Epitope: Not determined. Each clone binds within the

hydrophilic C-terminus of the cytoplasmic domain of                              Western blotting: Not recommended.

LMP-1.

                                                                                 * Recommended dilutions using VECTASTAIN庐 Elite庐

Presentation: Lyophilized tissue culture supernatant                             ABC Kits.

containing 15mM sodium azide. Reconstitute with 1 ml

of distilled water.                                                              Functional Aspects:

                                                                                 This pool of four monoclonal antibodies detects the

Storage Conditions:                                                              latent membrane protein (LMP-1) derived from the

Keep unopened vial at 2 - 8 掳C for 1 year. Once opened                           BNLF1 gene of Epstein-Barr virus (EBV). This antibody

it is recommended that reconstituted stock solution be                           cocktail will help detect EBV infected cells in normal

aliquoted and quick frozen and stored at -20 掳C. Do not                          and uncharacterized tissues.

repeatedly freeze/thaw.



Selected References

Murray P G, Young L S, Rowe M, et al.. Immunohistochemical demonstration of the Epstein-Barr virus-encoded latent membrane protein in paraffin

sections of Hodgkin鈥檚 disease. Journal of Pathology. 166: 1-5 (1992).

Herbst H, Dallenbach F, Hummel M, et al.. Epstein-Barr virus DNA and latent gene products in Ki-1 (CD30)-positive anaplastic large cell lymphoma.

Blood. 78: 1-10 (1991).

Jarrett R F, Gallagher A, Jones D B, et al.. Detection of Epstein-Barr virus genomes in Hodgkin鈥檚 disease: Relation to age. Journal of Clinical

Pathology. 44: 844-848 (1991).

Pallesen G, Hamilton-Dutoit S J, Rowe M, et al.. Expression of Epstein-Barr (EBV) latent gene products in tumor cells of Hodgkin鈥檚 disease. Lancet.

337: 320-322 (1991).

Haribuchi Y, Yamanaka N, Katura A, et al.. Epstein-Barr virus in nasal T-cell lymphomas in patients with lethal medline granuloma. Lancet. 335: 128-

130 (1990).

Young L, Alfieri C, Hennesy K, et al.. Expression of Epstein-Barr virus transformation-associated genes in tissue of patients with EBV lymphoprolifera-

tive disease. New England Journal of Medicine. 321: 1080-1085 (1989).

Fahraeus R, Li-Fu H, Ernberg I, et al.. Expression of the Epstein-Barr virus genome in nasopharyngeal carcinoma. International Journal of Cancer. 42:

329-338 (1988).

Rowe M, Evans H S, Young L S, et al.. Monoclonal antibodies to the latent membrane protein of Epstein-Barr virus reveal heterogeneity of the protein

and inducible expression in virus-transformed cells. Journal of General Virology. 68: 1575-1586 (1987).









VP-E609 is recommended FOR PROFESSIONAL AND RESEARCH USE ONLY.                                                                                    7/04







                 Vector Laboratories, Inc., 30 Ingold Road, Burlingame, CA 94010 U.S.A.

 Tel: (650)697-3600 鈥? Fax: (650)697-0339 鈥? Email: vector@vectorlabs.com 鈥? Web site: www.vectorlabs.com
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