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File Name: reactolab_ch---VPR151.asp
 
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               Respiratory syncytial virus                                                                                         VP-R151

Antibody to                                                                                                            Cat. No.



                                                         Product Specifications



Description:                                                                     Applications:

Respiratory syncytial virus mouse monoclonal anti-

                                                                                 Immunohistochemistry

bodies

                                                                                 Paraffin sections:       Antigen unmasking recommended.

Immunoglobulin class:                   5H5N - IgG1                              Frozen sections:         Yes

                                        2G122 - IgG1

                                                                                 Working Dilution*:       1:200 - 1:400 for 1 hr at 25掳C

                                        5A6 - IgG2a

                                                                                 Positive Control:        Formalin-fixed, paraffin-embedded

                                        1C3 - IgG2a

                                                                                                          RSV infected lung.

Clone:              5H5N, 2G122, 5A6 and 1C3                                     Indirect immunofluorescence

Immunogen:           Respiratory syncytial virus strain A2.                      Working Dilution*:       1:10

                                                                                                          See attached for protocol. Read

Epitope: Not determined.                                                                                  using immersion oil eg Cargille type

                                                                                                          FF (Product No. 12612).

Presentation: Lyophilized tissue culture supernatant                             Positive Control:        Acetone-fixed HeLa cells infected

containing 15mM sodium azide. Reconstitute with 1ml                                                       with RSV-A2. Staining pattern -

of distilled water.                                                                                       RSV-infected epithelial cell mem-

                                                                                                          branes and cytoplasm.

Storage Conditions:

                                                                                 Western blotting: Not recommended.

Keep unopened vial at 2 - 8 掳C for 1 year. Once opened

it is recommended that reconstituted stock solution be                           * Recommended dilutions using VECTASTAIN庐 Elite庐

aliquoted and quick frozen and stored at -20 掳C. Do not                          ABC Kits.

repeatedly freeze/thaw.

                                                                                 Functional Aspects:

                                                                                 Respiratory syncytial virus (RSV) is the most significant

                                                                                 respiratory pathogen of early development and causes

                                                                                 about half of all cases of bronchiolitis and a quarter of

                                                                                 all cases of pneumonia during the first few months of life.

                                                                                 There are multiple types and subtypes of RSV. VP-R151

                                                                                 is a mixture of four antibodies to optimize sensitivity and

                                                                                 detection of the various RSV forms. It does not cross-

                                                                                 react with tissue culture isolates of influenza virus types

                                                                                 A and B, parainfluenza virus types 1, 2, 3 and 4b, adeno-

                                                                                 virus, herpes simplex virus types 1 and 2, varicella-zoster

                                                                                 virus, cytomegalovirus, mumps virus, measles virus,

                                                                                 ECHOvirus 19, coxsackie B4 virus, poliovirus types 1, 2

                                                                                 and 3 or negative tissue culture cells used in routine

                                                                                 virus isolation.

Selected References

Wright C, Oliver K C, Fenwick F, et al.. A monoclonal antibody pool for routine immunohistochemical detection of human respiratory syncytial virus anti-

gens in formalin-fixed, paraffin-embedded tissue. Journal of Pathology. 182: 238-244 (1997).

Routledge E C, McQuillan J, Samson A C R, et al.. The development of monoclonal antibodies to respiratory syncytial virus and their use in diagnosis

by indirect immunofluorescence. Journal of Medical Virology. 15: 305-320 (1985).





VP-R151 is recommended FOR PROFESSIONAL AND RESEARCH USE ONLY.                                                                                    7/04





                 Vector Laboratories, Inc., 30 Ingold Road, Burlingame, CA 94010 U.S.A.

 Tel: (650)697-3600 鈥? Fax: (650)697-0339 鈥? Email: vector@vectorlabs.com 鈥? Web site: www.vectorlabs.com
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