LTIMSDS1
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MATERIAL SAFETY DATA SHEET
__________________________ Page 1 of 9
Revised 8/08/04
5X THERMO-X RT BUFFER Replaces (None)
INVITROGEN CORPORATION Printed 8/08/04
MSDS ID: 98701
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1. PRODUCT AND COMPANY INFORMATION
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INVITROGEN CORPORATION GIBCO PRODUCTS
1600 FARADAY AVE. INVITROGEN CORPORATION
CARLSBAD, CA 92008 3175 STALEY ROAD P.O. BOX 68
760/603-7200 GRAND ISLAND, NY 14072
716/774-6700
INVITROGEN CORPORATION INVITROGEN CORPORATION
3 FOUNTAIN DR. P.O. BOX 12-502
INCHINNAN BUSINESS PARK PENROSE
PAISLEY, PA4 9RF AUCKLAND 1135
SCOTLAND NEW ZEALAND
44-141 814-6100 64-9-579-3024
INVITROGEN CORPORATION INVITROGEN AUSTRALIA PTY LIMITED
2270 INDUSTRIAL ST. 2A/14 LIONEL ROAD
BURLINGTON, ONT MOUNT WAVERLY VIC 3149
CANADA L7P 1A1 AUSTRALIA
905/335-2255 1-800-331-627
EMERGENCY NUMBER (SPILLS, EXPOSURES): 301/431-8585 (24 HOUR)
800/451-8346 (24 HOUR)
NON-EMERGENCY INFORMATION: 800/955-6288
Product Name: 5X THERMO-X RT BUFFER
Stock Number: 98701
NOTE: If this product is a kit or is supplied with more than one material,
please refer to the MSDS for each component for hazard information.
Product Use:
These products are for laboratory research use only and are not intended for
human or animal diagnostics, therapeutic, or other clinical uses, unless
otherwise stated.
Synonyms:
Not available.
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2. COMPOSITION, INFORMATION ON INGREDIENTS
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The following list shows components of this product classified as hazardous
based on physical properties and health effects:
Component CAS No. Percent
DIMETHYL SULFOXIDE 67-68-5 3-7
TRIS 77-86-1 7 - 13
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MATERIAL SAFETY DATA SHEET
__________________________ Page 2 of 9
Revised 8/08/04
5X THERMO-X RT BUFFER Replaces (None)
INVITROGEN CORPORATION Printed 8/08/04
MSDS ID: 98701
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3. HAZARDS IDENTIFICATION
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***************************** EMERGENCY OVERVIEW ****************************
Warning!
Irritant.
May be harmful if swallowed.
Harmful if absorbed.
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Potential Health Effects:
Eye:
Contact with the eyes may cause moderate to severe eye injury. Eye
contact may result in tearing and reddening, but not likely to permanently
injure eye tissue. Temporary vision impairment (cloudy or blurred vision)
is possible.
Skin:
Can cause severe irritation, defatting, and dermatitis. Irritation effects
may last for hours or days but will not likely result in permanent damage.
Upon prolonged or repeated exposure, harmful if absorbed through the skin.
May cause minor systemic damage.
Inhalation:
Can cause severe respiratory irritation, dizziness, weakness, fatigue,
nausea, headache and possible unconsciousness.
Other possible symptoms include; wheezing and coughing due to pulmonary edema
(fluid build-up in lungs).
No toxicity expected from inhalation.
Ingestion:
Irritating to mouth, throat, and stomach. Can cause abdominal discomfort,
nausea, vomiting and diarrhea.
Ingestion of this product may result in central nervous system effects
including headache, sleepiness, dizziness, slurred speech and blurred vision.
May cause liver disorder (e.g., edema, proteinuria) and damage. Overexposure
may cause kidney damage.
May be harmful if swallowed. May cause systemic poisoning.
Chronic:
No data on cancer.
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4. FIRST AID MEASURES
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Eye:
Immediately flush eyes with plenty of water for at least 20 minutes
retracting eyelids often. Tilt the head to prevent chemical from
transferring to the uncontaminated eye. Get immediate medical attention
and monitor the eye daily as advised by your physician.
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MATERIAL SAFETY DATA SHEET
__________________________ Page 3 of 9
Revised 8/08/04
5X THERMO-X RT BUFFER Replaces (None)
INVITROGEN CORPORATION Printed 8/08/04
MSDS ID: 98701
______________________________________________________________________________
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4. FIRST AID MEASURES (CONT.)
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Skin:
Wash with soap and water. Remove contaminated clothing, launder
immediately, and discard contaminated leather goods. Get medical attention
immediately.
Inhalation:
Remove to fresh air. If breathing is difficult, have a trained individual
administer oxygen. If not breathing, give artificial respiration and have
a trained individual administer oxygen. Get medical attention immediately.
Ingestion:
Severely irritating. Do not induce vomiting. Seek medical attention
immediately. Drink 2 glasses of water or milk to dilute.
Note To Physician:
Treat symptomatically.
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5. FIRE FIGHTING MEASURES
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Flashpoint Deg C:
Not available.
Upper Flammable Limit %:
Not available.
Lower Flammable Limit %:
Not available.
Autoignition Temperature Deg C:
Not available.
Extinguishing Media:
Use alcohol resistant foam, carbon dioxide, dry chemical, or water spray
when fighting fires. Water or foam may cause frothing if liquid is burning
but it still may be a useful extinguishing agent if carefully applied to
the fire. Do not direct a water stream directly into the hot burning liquid.
DMSO undergoes a violent exothermic reaction on mixing with copper wool and
trichloroacetic acid. On mixing with potassium permanganate it will flash
instantaneously. It reacts violently with: acid halides, cyanuric chloride,
silicon tetrachloride, phosphorus trichloride and trioxide, thionyl chloride,
magnesium perchlorate, silver fluoride, methyl bromide, iodine pentafluoride,
nitrogen periodate, diborane, sodium hydride, perchloric and periodic acids.
When heated above its boiling point, DMSO degrades giving off formaldehyde,
methyl mercaptan, and sulfur dioxide.
Firefighting Techniques/Equipment:
Do not enter fire area without proper protection including self-contained
breathing apparatus and full protective equipment. Fight fire from a safe
distance and a protected location due to the potential of hazardous vapors
and decomposition products.
LTIMSDS2
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MATERIAL SAFETY DATA SHEET
__________________________ Page 4 of 9
Revised 8/08/04
5X THERMO-X RT BUFFER Replaces (None)
INVITROGEN CORPORATION Printed 8/08/04
MSDS ID: 98701
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5. FIRE FIGHTING MEASURES (CONT.)
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Hazardous Combustion Products:
Carbon dioxide Carbon monoxide Sulfur containing gases
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6. ACCIDENTAL RELEASE MEASURES
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Accidental releases may be subject to special reporting requirements and other
regulatory mandates. Refer to Section 8 for personal protection equipment
recommendations.
Spill Cleanup:
Exposure to the spilled material may be severely irritating or toxic.
Follow personal protective equipment recommendations found in Section VIII
of this MSDS. Personal protective equipment needs must be evaluated based
on information provided on this sheet and the special circumstances
created by the spill including; the material spilled, the quantity of the
spill, the area in which the spill occurred, and the expertise of
employees in the area responding to the spill. Never exceed any
occupational exposure limits.
Ventilate the contaminated area.
Prevent the spread of any spill to minimize harm to human health and the
environment if safe to do so. Wear complete and proper personal protective
equipment following the recommendation of Section VIII at a minimum. Dike
with suitable absorbent material like granulated clay. Gather and store in
a sealed container pending a waste disposal evaluation.
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7. HANDLING AND STORAGE
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Storage of some materials is regulated by federal, state, and/or local laws.
Storage Pressure:
Ambient
Handling Procedures:
Toxic or severely irritating material. Avoid contacting and avoid
breathing the material. Use only in a well ventilated area.
Keep closed or covered when not in use.
Storage Procedures:
Store in a cool dry ventilated location. Isolate from incompatible
materials and conditions. Keep container(s) closed.
Suitable for most general chemical storage areas.
LTIMSDS2
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MATERIAL SAFETY DATA SHEET
__________________________ Page 5 of 9
Revised 8/08/04
5X THERMO-X RT BUFFER Replaces (None)
INVITROGEN CORPORATION Printed 8/08/04
MSDS ID: 98701
______________________________________________________________________________
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8. EXPOSURE CONTROLS, PERSONAL PROTECTION
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Exposure Limits:
OSHA PEL AGCIH TWA
Component (ppm) (ppm)
DIMETHYL SULFOXIDE Not established. Not established.
TRIS Not established. Not established.
Engineering Controls:
Local exhaust ventilation or other engineering controls are normally
required when handling or using this product to avoid overexposure.
Personal Protective Equipment:
Eye:
An eye wash station must be available where this product is used.
Wear chemically resistant safety glasses with side shields when handling
this product. Wear additional eye protection such as chemical splash
goggles and/or face shield when the possibility exists for eye contact
with splashing or spraying liquid, or airborne material. Do not wear
contact lenses. Have an eye wash station available.
Skin:
Avoid skin contact by wearing chemically resistant gloves, an apron and
other protective equipment depending upon conditions of use. Inspect
gloves for chemical break-through and replace at regular intervals. Clean
protective equipment regularly. Wash hands and other exposed areas with
mild soap and water before eating, drinking, and when leaving work.
Gloves should be used as minimum hand protection.
Respiratory:
Use supplied-air respiratory equipment as required.
A respiratory protection program that meets OSHA's 29 CFR 1910.134 and
ANSI Z88.2 requirements must be followed whenever workplace conditions
warrant a respirator's use.
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9. PHYSICAL AND CHEMICAL PROPERTIES
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Appearance/physical state: Liquid solution / suspension
Odor: No odor.
Boiling Point (C): 100
Melting Point (C): 0
Solubility in water: 100%
pH: 7.0
Vapor Pressure: 0.0212 ATM AT 20掳C
Vapor Density: 17.3 G/M3
Specific Gravity/Density: 1 g/ml
Specific Gravity/Density: 1.00
Octanol/water Partition Coeff: Not established.
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MATERIAL SAFETY DATA SHEET
__________________________ Page 6 of 9
Revised 8/08/04
5X THERMO-X RT BUFFER Replaces (None)
INVITROGEN CORPORATION Printed 8/08/04
MSDS ID: 98701
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9. PHYSICAL AND CHEMICAL PROPERTIES (CONT.)
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Volatiles: Not established.
Evaporation Rate: 0.3 (BUTYL ACETATE = 1.0)
Viscosity: 0.98 cp at 20掳C
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10. STABILITY AND REACTIVITY
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Stability:
Stable under normal conditions.
Conditions to Avoid:
Strong oxidizing agents. Temperatures above the high flash point of this
combustible material in combination with sparks, open flames, or other sources
of ignition. Strong alkalies. DMSO undergoes a violent exothermic reaction
on mixing with copper wool and trichloroacetic acid. On mixing with potassium
permanganate it will flash instantaneously. It reacts violently with: acid
halides, cyanuric chloride, silicon tetrachloride, phosphorus trichloride and
trioxide, thionyl chloride, magnesium perchlorate, silver fluoride, methyl
bromide, iodine pentafluoride, nitrogen periodate, diborane, sodium hydride,
perchloric and periodic acids. When heated above its boiling point, DMSO
degrades giving off formaldehyde, methyl mercaptan, and sulfur dioxide.
Strong acids. Brass. Hypochlorites. Aldehydes.
Hazardous Decomposition Products:
Carbon monoxide. Carbon dioxide. Sulfur containing gases. Nitrogen oxides.
Hazardous Polymerization:
Hazardous polymerization will not occur.
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11. TOXICOLOGICAL INFORMATION
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Acute Toxicity:
Dermal/Skin:
DIMETHYL SULFOXIDE: 40 GM/KG
Inhalation/Respiratory:
Not determined.
Oral/Ingestion:
DIMETHYL SULFOXIDE: 14,500 MG/KG
Tris: 5900 MG/KG
Target Organs: Blood. Eyes. Skin.
Carcinogenicity:
LTIMSDS2
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MATERIAL SAFETY DATA SHEET
__________________________ Page 7 of 9
Revised 8/08/04
5X THERMO-X RT BUFFER Replaces (None)
INVITROGEN CORPORATION Printed 8/08/04
MSDS ID: 98701
______________________________________________________________________________
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11. TOXICOLOGICAL INFORMATION (CONT.)
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NTP:
Not tested.
IARC:
Not listed.
OSHA:
Not regulated.
Other Toxicological Information
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12. Ecological Information
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Ecotoxicological Information: No ecological information available.
Environmental Fate (Degradation, Transformation, and Persistence):
Bioconcentration is not expected to occur.
Biodegrades slowly.
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13. DISPOSAL CONSIDERATIONS
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Regulatory Information:
Not applicable.
Disposal Method:
Clean up and dispose of waste in accordance with all federal, state, and
local environmental regulations.
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14. TRANSPORT INFORMATION
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Proper Shipping Name: Not regulated.
Subsidiary Hazards:
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15. REGULATORY INFORMATION
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UNITED STATES:
TSCA:
This product is solely for research and development purposes only and may not
be used, processed or distributed for a commercial purpose. It may only be
handled by technically qualified individuals.
Materials used in manufacturing processes which are subject to compliance with
the Federal Food Drug and Cosmetic Act (FDA) are not subject to the Toxic
LTIMSDS2
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MATERIAL SAFETY DATA SHEET
__________________________ Page 8 of 9
Revised 8/08/04
5X THERMO-X RT BUFFER Replaces (None)
INVITROGEN CORPORATION Printed 8/08/04
MSDS ID: 98701
______________________________________________________________________________
______________________________________________________________________________
15. REGULATORY INFORMATION (CONT.)
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Substance Control Act (TSCA). The TSCA research use only restriction does not
apply to FDA regulated manufacturing processes nor the resulting product
per 15 U.S.C. 2602(2).
Prop 65 Listed Chemicals: PROP 65 PERCENT
No Prop 65 Chemicals.
No 313 Chemicals
CANADA:
DSL/NDSL:
Not determined.
COMPONENT WHMIS Classification
DIMETHYL SULFOXIDE D2B
TRIS Not classified as hazardous.
EUROPEAN UNION:
PRODUCT RISK PHRASES:
None assigned.
PRODUCT SAFETY PHRASES:
Not applicable.
PRODUCT CLASSIFICATION:
Not classified
XI
EINECS
Component Number
DIMETHYL SULFOXIDE 200-664-3
TRIS 201-064-4
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16. OTHER INFORMATION
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HMIS Rating 0-4:
FIRE: Not determined.
HEALTH: Not determined.
REACTIVITY: Not determined.
Abbreviations
N/A - Data is not applicable or not available
SARA - Superfund and Reauthorization Act
HMIS - Hazard Material Information System
WHMIS - Workplace Hazard Materials Information System
NTP - National Toxicology Program
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MATERIAL SAFETY DATA SHEET
__________________________ Page 9 of 9
Revised 8/08/04
5X THERMO-X RT BUFFER Replaces (None)
INVITROGEN CORPORATION Printed 8/08/04
MSDS ID: 98701
______________________________________________________________________________
______________________________________________________________________________
16. OTHER INFORMATION (CONT.)
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OSHA - Occupational Health and Safety Administration
IARC - International Agency for Research on Cancer
PROP 65 - California Safe Drinking Water and
Toxic Enforcement Act of 1986
EINECS - European Inventory of Existing Commercial
Chemical Substances
The above information was acquired by diligent search and/or investigation
and the recommendations are based on prudent application of professional
judgment. The information shall not be taken as being all inclusive and is
to be used only as a guide. All materials and mixtures may present unknown
hazards and should be used with caution. Since Invitrogen Corporation cannot
control the actual methods, volumes, or conditions of use, the Company shall
not be held liable for any damages or losses resulting from the handling or
from contact with the product as described herein. THE INFORMATION IN THIS
MSDS DOES NOT CONSTITUTE A WARRANTY, EXPRESS OR IMPLIED, INCLUDING ANY
IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE.
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