Kit components
10/30/2003
Product code Description
555214 TMB Substrate Reagent Set
Components:
555214a Substrate Reagent A (51-2606KC)
555214b Subtrate Reagent B (51-2607KC)
Page 1/5
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
1 Identification of substance:
路 Product details:
路 Product name: Substrate Reagent A (51-2606KC)
(TMB Substrate Reagent Set)
路 Catalog number: 555214
路 Manufacturer/Supplier:
BD Biosciences, Pharmingen
10975 Torreyana Road
San Diego, CA 92121-1106
Tel. (877)232-8995
路 Information department: Technical Services
路 Emergency information:
In case of a chemical emergency, spill, fire, exposure, or accident please call ChemTrec at (800)424-
9300 or 911
2 Composition/Data on components:
路 Chemical characterization
路 Description: Mixture consisting of the following components.
路 Dangerous components: Void
3 Hazards identification
路 Hazard description:
This product contains no hazardous constituents, or the concentration of all chemical constituents are
below the regulatory threshold limits described by Occupational Safety Health Administration Hazard
Communication Standard 29 CFR 1910.1200 and the European Directive 91/155/EEC, and 93/112/EC.
路 Classification system
The classification was made according to the latest editions of international substances lists, and
expanded upon from company and literature data.
路 NFPA ratings (scale 0-4)
Health = 0
0 Fire = 0
0 0 Reactivity = 0
4 First aid measures
路 General information No special measures required.
路 After inhalation Seek medical treatment in case of complaints.
路 After skin contact Immediately wash with water and soap and rinse thoroughly.
路 After eye contact
Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor.
路 After swallowing If symptoms persist consult doctor.
路 Information for doctor Show this label.
USA
(Contd. on page 2)
DR
Page 2/5
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
Product name: Substrate Reagent A (51-2606KC)
(TMB Substrate Reagent Set)
(Contd. of page 1)
5 Fire fighting measures
路 Suitable extinguishing agents
CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
路 Protective equipment: No special measures required.
6 Accidental release measures
路 Person-related safety precautions: Not required.
路 Measures for environmental protection: Wipe up with damp sponge or mop.
路 Measures for cleaning/collecting: No special measures required.
路 Additional information: No dangerous substances are released.
7 Handling and storage
路 Handling
路 Information for safe handling: No special measures required.
路 Information about protection against explosions and fires: No special measures required.
路 Storage
路 Requirements to be met by storerooms and receptacles: 4 C
路 Information about storage in one common storage facility: Store away from oxidizing agents.
路 Further information about storage conditions: Store in cool, dry conditions in well sealed containers.
8 Exposure controls and personal protection
路 Additional information about design of technical systems: No further data; see item 7.
路 Components with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be
monitored at the workplace.
路 Additional information: The lists that were valid during the creation were used as basis.
路 Personal Protective Equipment
路 General protective and hygienic measures
The usual precautionary measures for handling chemicals should be followed.
路 Breathing equipment: Not required.
路 Protection of hands:
Chemical resistant gloves (i.e. latex, nitrile, or equivalent).
路 Eye protection: Safety glasses
路 Body protection: Protective work clothing (lab coat).
USA
(Contd. on page 3)
DR
Page 3/5
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
Product name: Substrate Reagent A (51-2606KC)
(TMB Substrate Reagent Set)
(Contd. of page 2)
9 Physical and chemical properties:
路 General Information
Liquid
Form:
Clear
Color:
Characteristic
Odor:
Undetermined
路 Change in condition
路 Melting point/Melting range: Not determined
路 Boiling point/Boiling range: Not determined
Not applicable
路 Flash point:
Product is not self igniting.
路 Auto igniting:
Product does not present an explosion hazard.
路 Danger of explosion:
Not applicable
路 Vapor pressure:
1 g/cm鲁
路 Density at 20掳C (68掳F):
路 Solubility in / Miscibility with
Soluble
Water:
Not applicable
路 pH-value:
路 Solvent content:
98.8 %
Water:
10 Stability and reactivity
路 Thermal decomposition / conditions to be avoided:
No decomposition if used according to specifications.
路 Materials to be avoided: Incompatible material: strong oxidizers.
路 Dangerous reactions No dangerous reactions known
路 Dangerous products of decomposition: No dangerous decomposition products known.
11 Toxicological information
路 Acute toxicity:
路 Primary irritant effect:
路 on the skin: No irritating effect.
路 on the eye: No irritating effect.
路 Sensitization: No sensitizing effects known.
路 Additional toxicological information:
The product is not subject to OSHA classification according to internally approved calculation methods
for preparations.
When used and handled according to specifications, the product does not have any harmful effects
according to our experience and the information provided to us.
USA
(Contd. on page 4)
DR
Page 4/5
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
Product name: Substrate Reagent A (51-2606KC)
(TMB Substrate Reagent Set)
(Contd. of page 3)
12 Ecological information:
路 Ecotoxical effects:
路 Other information:
The ecological effects have not been thoroughly investigated, but currently none have been identified.
路 General notes: Generally not hazardous for water.
13 Disposal considerations
路 Product:
路 Recommendation
Smaller quantities can be disposed of with solid waste.
This product is not considered a RCRA hazardous waste.
Dispose of material in accordance with federal (40 CFR 261), state and local requirements.
路 Uncleaned packagings:
路 Recommendation: Disposal must be made according to state and federal regulations.
路 Recommended cleansing agent: Water, if necessary with cleansing agents.
14 Transport information
路 DOT regulations:
-
路 Hazard class:
路 Land transport ADR/RID (cross-border)
路 ADR/RID class: -
路 Maritime transport IMDG:
-
路 IMDG Class:
路 Marine pollutant: No
路 Air transport ICAO-TI and IATA-DGR:
路 ICAO/IATA Class: -
路 Transport/Additional information:
*** Note if a dashed line appears the Hazard Class section for each mode of transportion, this indicates
the product is non-regulated for transportation. If a Hazard Class number is listed the product is
regulated for transportation.
15 Regulations
路 SARA Section 355 (extremely hazardous substances)
None of the ingredients is listed.
路 SARA Section 313 (specific toxic chemical listings)
None of the ingredients is listed.
路 TSCA (Toxic Substances Control Act)
6131-90-4 sodium acetate trihydrate
77-92-9 citric acid
7772-99-8 stannous chloride
(Contd. on page 5)
USA
DR
Page 5/5
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
Product name: Substrate Reagent A (51-2606KC)
(TMB Substrate Reagent Set)
(Contd. of page 4)
7722-84-1 hydrogen peroxide
路 California Proposition 65 - Chemicals known to cause cancer
None of the ingredients is listed.
路 California Proposition 65 - Chemicals known to cause reproductive toxicity
None of the ingredients is listed.
路 Carcinogenicity categories
路 IARC (International Agency for Research on Cancer)
7722-84-1 hydrogen peroxide 3
路 NTP (National Toxicology Program)
None of the ingredients is listed.
路 TLV (Threshold Limit Value established by ACGIH)
7722-84-1 hydrogen peroxide A3
路 MAK (German Maximum Workplace Concentration)
None of the ingredients is listed.
路 Product related hazard informations:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations pertaining to regulations on hazardous materials.
路 National regulations
路 Water hazard class: Generally not hazardous for water.
16 Other information:
To the best of our knowledge, the information contained herein is accurate. However, neither
Becton,Dickinson and Company or any of its subsidiaries assumes any liabilities whatsoever for the
accuracy or completeness of the information contained herein. Final determination of suitability of any
material is the sole responsibility of the user. All materials may present unknown hazards and should be
used with caution. Although certain hazards are described herein, we cannot guarantee that these are
the only hazards that exist.
路 Department issuing MSDS: Safety and Environment Department
路 Contact: Technical Service Representative
USA
DR
Page 1/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
1 Identification of substance:
路 Product details:
路 Product name: Subtrate Reagent B (51-2607KC)
(TMB Substrate Reagent Set)
路 Catalog number: 555214
路 Manufacturer/Supplier:
BD Biosciences, Pharmingen
10975 Torreyana Road
San Diego, CA 92121-1106
Tel. (877)232-8995
路 Information department: Technical Services
路 Emergency information:
In case of a chemical emergency, spill, fire, exposure, or accident please call ChemTrec at (800)424-
9300 or 911
2 Composition/Data on components:
路 Chemical characterization
路 Description: Mixture consisting of the following components.
路 Dangerous components:
67-56-1 methanol 39.5%
67-68-5 dimethyl sulfoxide 1.5%
3 Hazards identification
路 Hazard description:
T Toxic
F Highly flammable
路 Information pertaining to particular dangers for man and environment
R 11 Highly flammable.
R 23/24/25 Toxic by inhalation, in contact with skin and if swallowed.
R 39 Danger of very serious irreversible effects.
R 39/23/24/25 Toxic: danger of very serious irreversible effects through inhalation, in contact with skin
and if swallowed.
路 Classification system
The classification was made according to the latest editions of international substances lists, and
expanded upon from company and literature data.
路 NFPA ratings (scale 0-4)
Health = 3
3 Fire = 3
3 0 Reactivity = 0
4 First aid measures
路 General information
Immediately remove contaminated clothing.
(Contd. on page 2)
USA
DR
Page 2/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
Product name: Subtrate Reagent B (51-2607KC)
(TMB Substrate Reagent Set)
(Contd. of page 1)
Remove breathing apparatus only after contaminated clothing have been completely removed.
In case of irregular breathing or respiratory arrest provide artificial respiration.
路 After inhalation
Supply fresh air or oxygen; and immediately call for a doctor.
In case of unconsciousness place patient on side position for transportation.
路 After skin contact Immediately wash with water and soap and rinse thoroughly.
路 After eye contact Rinse opened eye for 15 minutes under running water. Then consult a doctor.
路 After swallowing Do not induce vomiting; immediately call for medical help.
路 Information for doctor Show this label.
5 Fire fighting measures
路 Suitable extinguishing agents
CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
路 Protective equipment: No special measures required.
6 Accidental release measures
路 Person-related safety precautions: Not required.
路 Measures for environmental protection: Wipe up with damp sponge or mop.
路 Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders).
Dispose contaminated material as waste according to item 13.
Ensure adequate ventilation.
路 Additional information:
See Section 7 for information on safe handling.
See Section 8 for information on personal protection equipment.
See Section 13 for disposal information.
7 Handling and storage
路 Handling
路 Information for safe handling:
Ensure good ventilation/exhaustion at the workplace.
Open and handle receptacle with care.
路 Information about protection against explosions and fires:
Keep ignition sources away - Do not smoke.
Protect against electrostatic charges.
Keep respiratory protective device available.
路 Storage
路 Requirements to be met by storerooms and receptacles: 2 - 8 C
路 Information about storage in one common storage facility: Store away from oxidizing agents.
路 Further information about storage conditions: Store in cool, dry conditions in well sealed containers.
8 Exposure controls and personal protection
路 Additional information about design of technical systems: No further data; see item 7.
(Contd. on page 3)
USA
DR
Page 3/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
Product name: Subtrate Reagent B (51-2607KC)
(TMB Substrate Reagent Set)
(Contd. of page 2)
路 Components with limit values that require monitoring at the workplace:
67-56-1 methanol
PEL 260 mg/m鲁, 200 ppm
REL Short-term value: 325 mg/m鲁, 250 ppm
Long-term value: 260 mg/m鲁, 200 ppm
Skin
TLV Short-term value: 328 mg/m鲁, 250 ppm
Long-term value: 262 mg/m鲁, 200 ppm
Skin; BEI
56-81-5 glycerol
PEL 15*; 5** mg/m鲁
*Total dust **Respirable fraction
TWA Short-term value: 10 mg/m鲁
路 Additional information: The lists that were valid during the creation were used as basis.
路 Personal Protective Equipment
路 General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
Immediately remove all soiled and contaminated clothing.
Wash hands before breaks and at the end of work.
Store protective clothing separately.
Avoid contact with the eyes and skin.
路 Breathing equipment:
In case of brief exposure, use a chemical fume hood or a NIOSH/MSHA-approved respirator.
路 Protection of hands:
Chemical resistant gloves (i.e. latex, nitrile, or equivalent).
路 Eye protection:
Safety glasses
Tightly sealed goggles.
路 Body protection: Protective work clothing (lab coat).
9 Physical and chemical properties:
路 General Information
Liquid
Form:
Clear
Color:
Characteristic
Odor:
路 Change in condition
Melting point/Melting range: Not determined
Boiling point/Boiling range: Not determined
Not applicable
路 Flash point:
400.0掳C (752掳F)
路 Ignition temperature:
(Contd. on page 4)
USA
DR
Page 4/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
Product name: Subtrate Reagent B (51-2607KC)
(TMB Substrate Reagent Set)
(Contd. of page 3)
Product is not self igniting.
路 Auto igniting:
Product does not present an explosion hazard.
路 Danger of explosion:
路 Explosion limits:
0.9 Vol %
Lower:
44.0 Vol %
Upper:
Not applicable
路 Vapor pressure:
Not determined
路 Density:
路 Solubility in / Miscibility with
Soluble
Water:
Not applicable
路 pH-value:
路 Solvent content:
51.0 %
Organic solvents:
49.0 %
Water:
10 Stability and reactivity
路 Thermal decomposition / conditions to be avoided:
No decomposition if used according to specifications.
路 Materials to be avoided: Incompatible material: strong oxidizers.
路 Dangerous reactions No dangerous reactions known
路 Dangerous products of decomposition: No dangerous decomposition products known.
11 Toxicological information
路 Acute toxicity:
路 LD/LC50 values that are relevant for classification:
67-56-1 methanol
Oral LD50 5628 mg/kg (rat)
路 Primary irritant effect:
路 on the skin: No irritating effect.
路 on the eye: No irritating effect.
路 Sensitization: No sensitizing effects known.
路 Subacute to chronic toxicity: Target organs: kidneys
路 Additional toxicological information:
The product shows the following dangers according to internally approved calculation methods for
preparations:
Toxic
12 Ecological information:
路 Ecotoxical effects:
路 Other information:
The ecological effects have not been thoroughly investigated, but currently none have been identified.
(Contd. on page 5)
USA
DR
Page 5/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
Product name: Subtrate Reagent B (51-2607KC)
(TMB Substrate Reagent Set)
(Contd. of page 4)
路 General notes: Water hazard class 1 (Self-assessment): slightly hazardous for water.
13 Disposal considerations
路 Product:
路 Recommendation
Must not be disposed of with solid waste.
Dispose of material in accordance with federal (40 CFR 261), state and local requirements.
This product is not considered a RCRA hazardous waste.
路 Uncleaned packagings:
路 Recommendation: Disposal must be made according to state and federal regulations.
路 Recommended cleansing agent: Water, if necessary with cleansing agents.
14 Transport information
路 DOT regulations:
3
路 Hazard class:
UN1993
路 Identification number:
II
路 Packing group:
FLAMMABLE LIQUID, N.O.S. (METHANOL)
路 Proper shipping name (technical name):
3
路 Label
路 Land transport ADR/RID (cross-border)
3 (F1) Flammable liquids
路 ADR/RID class:
33
路 Danger code (Kemler):
1993
路 UN-Number:
II
路 Packaging group:
1993 FLAMMABLE LIQUID, N.O.S., special provision 640D
路 Description of goods:
(METHANOL)
路 Maritime transport IMDG:
3
路 IMDG Class:
1993
路 UN Number:
3
路 Label
II
路 Packaging group:
F-E,S-E
路 EMS Number:
(Contd. on page 6)
USA
DR
Page 6/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
Product name: Subtrate Reagent B (51-2607KC)
(TMB Substrate Reagent Set)
(Contd. of page 5)
No
路 Marine pollutant:
FLAMMABLE LIQUID, N.O.S. (METHANOL)
路 Proper shipping name:
路 Air transport ICAO-TI and IATA-DGR:
3
路 ICAO/IATA Class:
1993
路 UN/ID Number:
3
路 Label
II
路 Packaging group:
FLAMMABLE LIQUID, N.O.S. (METHANOL)
路 Proper shipping name:
路 Transport/Additional information:
*** Note if a dashed line appears the Hazard Class section for each mode of transportion, this indicates
the product is non-regulated for transportation. If a Hazard Class number is listed the product is
regulated for transportation.
15 Regulations
路 SARA Section 355 (extremely hazardous substances)
None of the ingredients is listed.
路 SARA Section 313 (specific toxic chemical listings)
67-56-1 methanol
路 TSCA (Toxic Substances Control Act)
67-56-1 methanol
56-81-5 glycerol
67-68-5 dimethyl sulfoxide
54827-17-7 3,3',5,5'-tetramethylbenzidine
路 California Proposition 65 - Chemicals known to cause cancer
None of the ingredients is listed.
路 California Proposition 65 - Chemicals known to cause reproductive toxicity
None of the ingredients is listed.
路 Carcinogenicity categories
路 IARC (International Agency for Research on Cancer)
None of the ingredients is listed.
路 NTP (National Toxicology Program)
None of the ingredients is listed.
路 TLV (Threshold Limit Value established by ACGIH)
None of the ingredients is listed.
路 MAK (German Maximum Workplace Concentration)
None of the ingredients is listed.
(Contd. on page 7)
USA
DR
Page 7/7
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/30/2003 Reviewed on 10/30/2003
Product name: Subtrate Reagent B (51-2607KC)
(TMB Substrate Reagent Set)
(Contd. of page 6)
路 Product related hazard informations:
The product has been classified and marked in accordance with regulations on hazardous materials.
路 Hazard symbols:
T Toxic
F Highly flammable
路 Hazard-determining components of labelling:
methanol
dimethyl sulfoxide
路 Risk phrases:
11 Highly flammable.
23/24/25 Toxic by inhalation, in contact with skin and if swallowed.
39 Danger of very serious irreversible effects.
39/23/24/25 Toxic: danger of very serious irreversible effects through inhalation, in contact with skin
and if swallowed.
路 Safety phrases:
7/9 Keep container tightly closed and in a well-ventilated place.
16 Keep away from sources of ignition - No smoking.
25 Avoid contact with eyes.
26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
28 After contact with skin, wash immediately with plenty of water.
36/37/39 Wear suitable protective clothing, gloves and eye/face protection.
45 In case of accident or if you feel unwell, seek medical advice immediately.
60 This material and its container must be disposed of as hazardous waste.
路 National regulations
路 Technical instructions (air):
Class Share in %
Water 49.0
III 39.5
NK 11.5
路 Water hazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.
16 Other information:
To the best of our knowledge, the information contained herein is accurate. However, neither
Becton,Dickinson and Company or any of its subsidiaries assumes any liabilities whatsoever for the
accuracy or completeness of the information contained herein. Final determination of suitability of any
material is the sole responsibility of the user. All materials may present unknown hazards and should be
used with caution. Although certain hazards are described herein, we cannot guarantee that these are
the only hazards that exist.
路 Department issuing MSDS: Safety and Environment Department
路 Contact: Technical Service Representative
USA
DR
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