EMS CATALOG NO: 20800
EMS PRODUCT: Safranine O
DATE: June 24, 2001
PAGE NUMBER: One of 7
MATERIAL SAFETY DATA SHEET
The information contained herein is based on data considered
accurate. However, no warranty is expressed or implied
regarding the accuracy of these data or the results to be
obtained from the use thereof.
Electron Microscopy Sciences assumes no responsibility for
personal injury or property damage to vendees, users or
third parties caused by the material. Such vendees or users
assume all risks associated with the use of the material.
ELECTRON MICROSCOPY SCIENCES
321 MORRIS ROAD
P.O. BOX 251
FORT WASHINGTON, PA 19034 24 HOUR EMERGENCY PHONE NUMBER
(215) 646-1566 CHEMTREC: (800) 424-9300
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FOR PRODUCT AND SALES INFORMATION
_________________________________________________________________
CONTACT ELECTRON MICROSCOPY SCIENCES OFFICE ABOVE.
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PRODUCT IDENTIFICATION
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COMMON NAME: Safranine O
COMMERCIAL NAME(S): Basic Red 2
SYNONYM: 3,7-diamino-2, 8-dimethyl-5-phenylphenazinium chloride
CHEMICAL NAME:
CHEMICAL FAMILY: Not available.
CHEMICAL FORMULA: C20H19C1N4
CAS#: 477-73-6
RTECS: SG1623000
TSCA: On the TSCA list
CI#: 50240
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COMPOSITION AND INFORMATION ON INGREDIENTS
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NAME: Safranine O
CAS#: 477-73-6
% BY WEIGHT: 100
TOXICOLOGICAL DATA ON INGREDIENTS: Not applicable
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HAZARDS IDENTIFICATION
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POTENTIAL ACUTE HEALTH EFFECTS:
Slightly dangerous to dangerous in case of ingestion. Very
slightly to slightly dangerous in case of eye contact
(irritant), of inhalation.
POTENTIAL CHRONIC HEALTH EFFECTS:
Slightly dangerous to dangerous in case of ingestion.
Very slightly to slightly dangerous in case of eye contact
(irritant), of inhalation.
CARCINOGENIC EFFECTS: Not available.
MUTAGENIC EFFECTS: Not available.
TERATOGENIC EFFECTS: Not available.
Toxicity of the product to the reproductive system: Not
available.
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FIRST AID MEASURES
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EYE CONTACT: NO known EFFECT on eye contact, rinse with water for
a few minutes.
SKIN CONTACT: NO know EFFECT according to our database.
SERIOUS SKIN CONTACT: No additional information.
INHALATION: Allow the victim to rest in a well ventilated area.
Seek immediate medical attention.
SERIOUS INHALATION: No additional information.
INGESTION:
Remove dentures if any. Have conscious person drink several
glasses of water or milk. INDUCE VOMITING by sticking
2
finger in throat. Lower the head so that the vomit will not
reenter the mouth and throat. NEVER give an unconscious
person anything to ingest. Seek medial attention.
SERIOUS INGESTION: No additional information.
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FIRE AND EXPLOSION DATA
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FLAMMABILITY OF THE PRODUCT: Combustible.
AUTO-IGNITION TEMPERATURE: Not available.
FLASH POINTS: Not available.
FLAMMABLE LIMITS: Not available.
PRODUCTS OF COMBUSTION: These products are carbon oxides (CO, CO2),
nitrogen oxides (NO,NO2...), halogenated compounds.
FIRE HAZARDS IN PRESENCE OF VARIOUS SUBSTANCES: No specific
information is available in our database regarding the
flammability of this product in presence of various
materials.
EXPLOSION HAZARDS IN PRESENCE OF VARIOUS SUBSTANCES:
RISKS OF EXPLOSION OF THE PRODUCT IN PRESENCE OF
MECHANICAL
IMPACT: Not available.
RISKS OF EXPLOSION OF THE PRODUCT IN PRESENCE OF STATIC
DISCHARGE: Not available.
No specific information is available in our database
regarding the product's risks of explosion in the presence
of various materials.
FIRE FIGHTING MEDIA AND INSTRUCTIONS:
SMALL FIRE: Use DRY chemicals, CO2, water spray or foam.
LARGE FIRE: Use water spray, fog or foam. DO NOT use water jet.
SPECIAL REMARKS ON FIRE HAZARDS: No additional remark.
SPECIAL REMARKS ON EXPLOSION HAZARDS: No additional remark.
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ACCIDENTAL RELEASE MEASURES
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SMALL SPILL:
Use appropriate tools to put the spilled solid in a
convenient waste disposal container. Finish cleaning by
spreading water on the contaminated surface and dispose of
according to local and regional authority requirements.
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LARGE SPILL:
Our database contains no additional information in case of a
spill and/or a leak of the product. Use a shovel to put the
material into a convenient waste disposal container. Finish
cleaning by spreading water on the contaminated surface and
allow to evacuate through the sanitary system.
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HANDLING AND STORAGE
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PRECAUTIONS:
Keep away from heat. Keep away from sources of ignition.
Empty containers pose a fire risk, evaporate the residue
under a fume hood. Ground all equipment containing
material. DO NOT breathe dust.
STORAGE:
Keep container dry. Keep in a cool place. Ground all
equipment containing material. Keep container tightly
closed. Keep in a cool, well-ventilated place. Combustible
materials should be stored away from extreme heat and away
from strong oxidizing agents.
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EXPOSURE CONTROLS/PERSONAL PROTECTION
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ENGINEERING CONTROLS:
Use process enclosures, local exhaust ventilation, or other
engineering controls to keep airborne levels below
recommended exposure limits. If user operations generate
dust, fume or mist, use ventilation to keep exposure to
airborne contaminants below the exposure limit.
PERSONAL PROTECTION: Safety glasses. Lab coat.
PERSONAL PROTECTION IN CASE OF A LARGE SPILL:
Splash goggles. Full suit. Boots. Gloves. Suggested
protective clothing might not be sufficient; consult a
specialist BEFORE handling this product.
EXPOSURE LIMITS: Not available.
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PHYSICAL AND CHEMICAL PROPERTIES
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PHYSICAL STATE AND APPEARANCE: Solid.
ODOR: Not available.
TASTE: Not available.
COLOR: Not available.
MOLECULAR WEIGHT: 350.85
pH (1% SOLN/WATER): Not available.
BOILING POINT: Not available.
MELTING POINT: Decomposes.
CRITICAL TEMPERATURE: Not available.
SPECIFIC GRAVITY: Not available.
VAPOR PRESSURE: Not available.
VAPOR DENSITY: Not available.
VOLATILITY: Not available.
ODOR THRESHOLD: Not available.
WATER/OIL DIST. COEFF.: Not available..
IONICITY (IN WATER): Not available.
DISPERSION PROPERTIES: Not available.
SOLUBILITY: Not available.
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STABILITY AND REACTIVITY DATA
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STABILITY: The product is stable.
INSTABILITY TEMPERATURE: Not available.
CONDITIONS OF INSTABILITY: No additional remark.
INCOMPATIBILITY WITH VARIOUS SUBSTANCES: No specific information
is available in our database regarding the reactivity of
this material in presence of various other materials.
CORROSIVITY: Non-corrosive in presence of glass.
SPECIAL REMARKS ON REACTIVITY: No additional remark.
SPECIAL REMARKS ON CORROSIVITY: No additional remark.
POLYMERIZATION: No.
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TOXICOLOGICAL INFORMATION
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ROUTES OF ENTRY: Ingestion.
TOXICITY TO ANIMALS:
LD50: Not available.
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LC50: Not available.
CHRONIC EFFECTS ON HUMANS: Toxicity of the reproductive system:
Not available.
OTHER TOXIC EFFECTS ON HUMANS: Slightly dangerous to dangerous in
case of ingestion. Very slightly to slightly dangerous in
case of eye contact (irritant), of inhalation.
SPECIAL REMARKS ON TOXICITY TO ANIMALS: No additional remark.
SPECIAL REMARKS ON CHRONIC EFFECTS ON HUMANS: No additional remark.
SPECIAL REMARKS ON OTHER TOXIC EFFECTS ON HUMANS: No additional
remark.
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ECOLOGICAL INFORMATION
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ECOTOXICITY: Not available.
BOD5 AND COD: Not available.
PRODUCTS OF BIODEGRADATION: These products are carbon oxides (CO,
CO2), nitrogen oxides (NO, NO2...), halogenated compounds.
TOXICITY OF THE PRODUCTS OF BIODEGRADATION: The products of
degradation are more toxic.
SPECIAL REMARKS ON THE PRODUCTS OF BIODEGRADATION: No additional
remark.
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DISPOSAL CONSIDERATIONS
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WASTE DISPOSAL: Recycle to process, if possible. Consult your
local or regional authorities.
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TRANSPORT INFORMATION
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DOT CLASSIFICATION: Not a DOT controlled material (United States).
IDENTIFICATION: Not applicable (PIN and PG).
SPECIAL PROVISIONS FOR TRANSPORT: Not applicable.
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OTHER REGULATORY INFORMATION
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FEDERAL AND STATE REGULATIONS: The following product is listed on
TSCA: Safranine O
OTHER REGULATIONS:
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CANADIAN ENVIRONMENTAL PROTECTION ACT (CEPA): This product
is on the Domestic Substances List (DSL), and is acceptable
for use under the provisions of CEPA.
OTHER CLASSIFICATIONS:
WHMIS (CANADA): Not controlled under WHMIS (Canada).
DSCLA (EEC): Not controlled under DSCL (Europe).
HMIS (U.S.A.):
HEALTH HAZARD: 1
FIRE HAZARD: 1
REACTIVITY: 0
PERSONAL PROTECTION: a
NATIONAL FIRE PROTECTION ASSOCIATION (U.S.A.)
HEALTH:
FLAMMABILITY: 1
REACTIVITY: 0
SPECIFIC HAZARD:
PROTECTIVE EQUIPMENT:
Lab coat.
Safety glasses.
REFERENCES: Not available.
OTHER SPECIAL CONSIDERATIONS: No additional remark.
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