Material Safety Data Sheet

   Printing date: 27/04/2004                         Reviewed on: 27/04/2004                                   Page 1/3



1. Identification of the substance/preparation and of the company/undertaking



   �    Product details

   �    Component name:                              2X Reaction Buffer

   �                                                 qPCR MasterMix Plus for SYBR® green I 7,5 ml

        Product name:

                                                                                 ®

                                                     qPCR MasterMix Plus for SYBR green I 15 ml

                                                     qPCR MasterMix Plus for SYBR® green I 50 ml

                                                                                 ®

                                                     qPCR MasterMix Plus for SYBR green I Sample

   �    Catalog number:                              RT-SN2X-03+

                                                     RT-SN2X-06+

                                                     RT-SN2X-20+

                                                     RT-SN2X-005+



   �    Manufacturer/Supplier:                       Eurogentec S.A.

                                                     LIEGE SCIENCE PARK

                                                     B-4102 Seraing

                                                     Belgium

                                                     Tel. +32-4-3727400

                                                     Fax.+32-4-3727500



   �    Further information obtainable from:         Eurogentec Helpdesk

                                                     Tel. +32-4-3727665



   �    Information in case of emergency:            Please contact the regional Eurogentec representation in your

                                                     country or Eurogentec S.A. directly.



   �    MSDS-Nr.:                                    MSDS-2XRBSGHGSR+A-V1



2. Composition/information on ingredients



   �    Chemical characterization

   �    Description:                                 Mixture of substances listed below with non hazardous additions

   �    Dangerous components:



   56-81-5             Glycerol                                                                                <10%

   67-68-5             Dimethyl sulfoxide                                                                      <20%



3. Hazards identification



   �    Hazard description:                          Not applicable

   �    Information concerning particular hazards

        for human and environment:                   Warning � This preparation contains a substance not yet

                                                     tested completely.

   �    Classification system:                       The classification is according to the latest editions of the EU-lists,

                                                     and extended by company and literature data.



4. First-aid measures



   �    After inhalation:                            Fresh air. Consult a doctor in case of complaints.

   �    After skin contact:                          Wash off under running water

   �    After eye contact:                           Rinse out with plenty of water

   �    After swallowing:                            Call for a doctor immediately.



5. Fire-fighting measures



   �    Suitable extinguishing agents:               Water, CO2, powder.

   �    Protective equipment:                        Do not inhale explosion gases or combustion gases.

                                            Material Safety Data Sheet

   Printing date: 27/04/2004                            Reviewed on: 27/04/2004                            Page 2/3



Component name: 2X Reaction Buffer



6. Accidental release measures



   �    Person-related safety precautions:              Ensure adequate ventilation.

   �    Measures for environmental protection:          Do not allow product to reach sewage system or any water

                                                        course.

   �    Measures for cleaning/collection:               Absorb with liquid-binding material.



7. Handling and storage



   �    Handling:

   �    Information for safe handling:                  Not required

   �    Information about fire:                         Not required



   �    Storage:

   �    Requirements to be met by storerooms

        and receptacles:                                No further requirements

   �    Information about storage in one common

        storage facility:                               Not required

   �    Further information about storage conditions:   -20˚C

   �    Class according to regulation on flammable

        Liquids:                                        Void



8. Exposure controls/personal protection



   �    Additional information about design of

        technical facilities:                           No further requirements; see item 7.

   �    Ingredients with limit values that require

        monitoring at the workplace:                    No ingredients need monitoring

   �    Personal protective equipment:

   �    General protective and hygienic measures:       The usual precautionary measures are to be adhered when

                                                        handling chemicals

   �    Respiratory protection:                         Not required

   �    Protection of hands:                            Protective gloves

   �    Eye protection:                                 Protective goggles



9. Physical and chemical properties



   �    Form:                                           Fluid

   �    Color:                                          Colorless - Pink

   �    Odor:                                           Characteristic

   �    Change in condition

             Melting point/range:                       Undetermined

             Boiling point/range:                       Undetermined

   �    Flash point:                                    Not applicable

   �    Self-igniting:                                  Not self-igniting

   �    Danger of explosion:                            No explosion hazard

   �    Density:                                        Not determined

   �    Solubility in water:                            Fully soluble

   �    Miscibility with water:                         Fully miscible



10. Stability and reactivity



   �    Thermal decomposition/conditions to be avoided: No decomposition if used according to specifications.

   �    Dangerous reactions:                            Acids, alkalis, oxides and strong oxidizing agents.

   �    Dangerous decomposition products:               No dangerous decomposition products in hazardous amounts.

                                           Material Safety Data Sheet

   Printing date: 27/04/2004                             Reviewed on: 27/04/2004                                Page 3/3



Component name: 2X Reaction Buffer



11. Toxicological information

   �

   �    Acute toxicity:                                  -

   �    Primary irritant effect:

   �    On the skin:                                     Irritant to skin and mucous membranes

   �    On the eye:                                      Irritating effect

   �    Sensitization:                                   Possible

   �    Additional toxicological information:            The product should be handled with the usual care when dealing

                                                         with chemicals.



12. Ecological information



   �    General notes:                                   Slighhtly hazardous for water.



13. Disposal considerations



   �    Product:

   �    Recommendation:                                  Dispose as special waste in compliance with official regulations.

                                                         Do not allow product to reach sewage system.



   �    Uncleaned packaging:

   �    Recommendation:                                  Dispose in compliance with official regulations

   �    Recommended cleansing agents:                    Water, if necessary together with cleansing agents.



14. Transport information



   �    Land transport ADR/RID (cross-border)

   �    ADR/RID class:                                   -



   �    Maritime transport IMDG:

   �    IMDG Class:                                      -

   �    Marine pollutant:                                No



   �    Air transport ICAO-TI and IATA-DGR:

   �    ICAO/IATA Class:                                 -



15. Regulation information



   �    Labeling according to EU guidelines:

   �    Symbol                                           -

   �    R-phrases                                        -

   �    S-phrases                                        -



16. Other information



   This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific

   product features and shall not establish a legally valid contractual relationship.



   SYBR® is a registered trademark of Molecular Probes Inc.